| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,878,496 | C→T | A46T (GCA→ACA) | atpG ← | F0F1 ATP synthase subunit gamma |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,878,496 | 0 | C | T | 99.0% | 762.2 / ‑2.7 | 200 | A46T (GCA→ACA) | atpG | F0F1 ATP synthase subunit gamma |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (110/88); total (111/89) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01 | |||||||||||
Only 100 of 216 total aligned reads displayed.
TCTTCCAGGTAAGGGTGCTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGCATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTT > REL606/3878423‑3878568 | tCTTCCAGGTAAGGGTGCTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTAt < 1:1206900/76‑1 (MQ=255)tCTTCCAGGTAAGGGTGCTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTAt > 1:3105333/1‑76 (MQ=255) cTTCCAGGTAAGGGTGCTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGtata < 2:3485327/76‑1 (MQ=255) cTTCCAGGTAAGGGTGCTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGtata > 1:755013/1‑76 (MQ=255) cTTCCAGGTAAGGGTGCTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGtata < 1:3160217/76‑1 (MQ=255) ttCCAGGTAAGGGTGCTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATaa > 2:7343267/1‑76 (MQ=255) ttCCAGGTAAGGGTGCTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATaa < 2:3170003/76‑1 (MQ=255) tCCAGGTAAGGGTGCTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAg > 1:5114578/1‑76 (MQ=255) tCCAGGTAAGGGTGCTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAg < 1:5746324/76‑1 (MQ=255) tCCAGGTAAGGGTGCTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAg < 2:1769615/76‑1 (MQ=255) tCCAGGGAAGGGTGCTTATATTCCAGATTACCGTGTGCAAGGTGACAAATCACTTTCCGCATGGTTTCTGTATAAg > 2:4989795/1‑76 (MQ=255) ccAGGTAAGGGTGCTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAgg < 2:7240547/76‑1 (MQ=255) cAGGTAAGGGTGCTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGa > 1:5160837/1‑76 (MQ=255) aGGTAAGGGTGCTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGAc < 1:6092213/76‑1 (MQ=255) aGGTAAGGGTGCTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGAc < 1:6412460/76‑1 (MQ=255) aGGTAAGGGTGCTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGAc < 1:6693394/76‑1 (MQ=255) aGGTAAGGGTGCTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGAc < 2:4747160/76‑1 (MQ=255) aGGTAAGGGTGCTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGAc < 1:4834311/76‑1 (MQ=255) gTAAGGGTGCTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACgg > 2:1718573/1‑76 (MQ=255) tAAGGGTGCTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGc > 1:863415/1‑76 (MQ=255) aaGGGTGCTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCt > 2:4325592/1‑76 (MQ=255) aaGGGTGCTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCt > 1:7110030/1‑76 (MQ=255) aaGGGTGCTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCt > 1:4601569/1‑76 (MQ=255) aGGGTGCTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTg < 1:4018471/76‑1 (MQ=255) aGGGTGCTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTg > 2:2031529/1‑76 (MQ=255) aGGGTGCTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTg > 2:2885369/1‑76 (MQ=255) aGGGTGCTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTg < 2:4007841/76‑1 (MQ=255) aGGGTGCTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTg > 2:5490483/1‑76 (MQ=255) gTGCTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGgcc > 2:478186/1‑76 (MQ=255) cTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGgccgcc > 2:3291299/1‑76 (MQ=255) cTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGgccgcc > 1:2023881/1‑76 (MQ=255) cTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGgccgcc > 1:24683/1‑76 (MQ=255) tataTTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCAt < 2:4825641/76‑1 (MQ=255) atatTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATg < 1:7271923/76‑1 (MQ=255) tatTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGc < 2:5795025/76‑1 (MQ=255) tatTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGc > 2:6335867/1‑76 (MQ=255) atTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCg > 1:4451090/1‑76 (MQ=255) ttCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGa > 1:229710/1‑76 (MQ=255) ttCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGa > 1:1964329/1‑76 (MQ=255) tCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGAt > 1:1007746/1‑76 (MQ=255) ccAGATTACCGTGTACAAGGTGACCAATCACTCTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATc > 1:365839/1‑76 (MQ=255) cAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATcc < 1:7470736/76‑1 (MQ=255) aGATTACCGTGTGCAAGGTGACCAATCACTTTGCTCATGGTTTCTGTATAAGGAGGGCTGGCCGCCATGCGATCCt < 2:6536965/76‑1 (MQ=255) aGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCt < 2:7640759/76‑1 (MQ=255) aGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCt > 1:6912795/1‑76 (MQ=255) aGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCt < 1:5399088/76‑1 (MQ=255) gATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTg < 2:7154697/76‑1 (MQ=255) gATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTg < 1:6444877/76‑1 (MQ=255) aTTCCCGTTTGCAAGGTGACCAATCACTTTGCGGATGTTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGc < 2:2279358/76‑1 (MQ=255) aTTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGGACGCCATGCGATCCTGc > 2:2789083/1‑76 (MQ=255) ttACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCg < 2:212892/76‑1 (MQ=255) tACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGa > 2:5918691/1‑76 (MQ=255) tACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGa < 1:2231818/76‑1 (MQ=255) tACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGa > 2:4392445/1‑76 (MQ=255) aCCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGAt < 1:1526746/76‑1 (MQ=255) aCCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGAt > 1:531861/1‑76 (MQ=255) aCCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGAt > 2:3689945/1‑76 (MQ=255) aCCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCCAt > 2:1138671/1‑76 (MQ=255) ccGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGAGAtt > 1:3559308/1‑76 (MQ=255) cGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGAttt < 1:7014182/76‑1 (MQ=255) gtgtGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTa > 1:4417337/1‑76 (MQ=255) gtgtGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTa > 2:662771/1‑76 (MQ=255) gtgCAAGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACg > 1:3795070/1‑76 (MQ=255) aaGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCACGCGATTTACGCAtt > 2:3598610/1‑76 (MQ=255) aGGTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCAttt > 2:1307320/1‑76 (MQ=255) gTGACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTg < 2:5282666/76‑1 (MQ=255) gACCAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGa < 2:6973133/76‑1 (MQ=255) cAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAgc < 2:2397588/76‑1 (MQ=255) cAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAgc > 1:5559530/1‑76 (MQ=255) cAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAgc > 1:912088/1‑76 (MQ=255) cAATCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCAGCCCTGCGATCCTGCGATTTATGCATTTTGGCAgc > 1:2777362/1‑76 (MQ=255) aaTCACTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAgcg > 1:1697651/1‑76 (MQ=255) cACTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGTCATGCGATCCTGCGATTTACGCATTTTGGAAgcggcg < 1:3968256/76‑1 (MQ=255) cACTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAgcggcg < 2:1073767/76‑1 (MQ=255) aCTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGa > 1:4978834/1‑76 (MQ=255) aCTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCCGCGa > 1:5847069/1‑76 (MQ=255) cTTTGCGCATGTTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGAc < 2:3847185/76‑1 (MQ=255) cTTTGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGAc > 2:1236382/1‑76 (MQ=255) tttGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGAcc < 2:2649738/76‑1 (MQ=255) ttGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCa > 2:7603684/1‑76 (MQ=255) ttGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCa < 2:1558311/76‑1 (MQ=255) ttGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCa > 1:7056146/1‑76 (MQ=255) tGCGCATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCAt > 1:307900/1‑76 (MQ=255) cgcATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCAtct < 2:5708443/76‑1 (MQ=255) cgcATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCAtct < 2:6416922/76‑1 (MQ=255) gcATGGTTTCTGAATAAGGACGGCTGCCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCAtctc < 2:6126531/76‑1 (MQ=255) cATGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTcc > 2:4544857/1‑76 (MQ=255) aTGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTccc > 2:2300210/1‑75 (MQ=255) aTGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTTCa > 2:7475442/1‑76 (MQ=255) aTGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCa < 1:1551401/76‑1 (MQ=255) tgttttCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCAt < 1:254391/73‑1 (MQ=255) tGGTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGGCCATCTCCAt > 2:1591920/1‑76 (MQ=255) ggTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATc < 1:7655198/76‑1 (MQ=255) gTTTCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCg < 1:1414915/76‑1 (MQ=255) tttCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCACCTCCATCGc > 1:2153720/1‑76 (MQ=255) ttCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCCTCGCt > 2:4358599/1‑76 (MQ=255) tCTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCtt < 2:5625015/76‑1 (MQ=255) cTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCttt < 2:6906915/76‑1 (MQ=255) cTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCttt < 2:5945651/76‑1 (MQ=255) cTGTATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCttt < 1:3411063/76‑1 (MQ=255) | TCTTCCAGGTAAGGGTGCTTATATTCCAGATTACCGTGTGCAAGGTGACCAATCACTTTGCGCATGGTTTCTGCATAAGGACGGCTGGCCGCCATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTT > REL606/3878423‑3878568 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |