| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,163,752 | T→G | I966S (ATC→AGC) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,163,752 | 0 | T | G | 99.3% | 572.9 / NA | 135 | I966S (ATC→AGC) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (93/41); minor base C (0/1); total (93/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 158 total aligned reads displayed.
GACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGA > REL606/4163679‑4163824 | gACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGcc < 2:7574048/76‑1 (MQ=255)gACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGcc > 1:5053577/1‑76 (MQ=255) aCAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCt < 1:790406/76‑1 (MQ=255) aCAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCt < 2:6724697/76‑1 (MQ=255) aCAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCt > 2:4694160/1‑76 (MQ=255) aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCg < 2:648931/76‑1 (MQ=255) cGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAg > 2:7224022/1‑76 (MQ=255) tGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCg < 2:2603875/76‑1 (MQ=255) tGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCg < 1:316943/76‑1 (MQ=255) gcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGt > 1:2098427/1‑76 (MQ=255) gcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGt < 1:2202439/76‑1 (MQ=255) cTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTc > 1:4923706/1‑76 (MQ=255) cTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTc > 2:1796647/1‑76 (MQ=255) cTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTc > 2:3986885/1‑76 (MQ=255) tGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCt < 2:5987436/76‑1 (MQ=255) tGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCt > 2:5589085/1‑76 (MQ=255) tGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCt > 2:1369184/1‑76 (MQ=255) tGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCt < 1:6415919/76‑1 (MQ=255) ggAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTg < 2:7640588/76‑1 (MQ=255) ggAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTg > 1:4467493/1‑76 (MQ=255) ggAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTg > 2:356114/1‑76 (MQ=255) gAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCttt < 2:3989386/76‑3 (MQ=255) gAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGt > 2:7039827/1‑76 (MQ=255) aaTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTc > 2:6667880/1‑76 (MQ=255) aaTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTc < 2:6344531/76‑1 (MQ=255) aTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCa < 1:4132946/76‑1 (MQ=255) aTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCa > 1:4589913/1‑76 (MQ=255) tCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAg < 2:2741157/76‑1 (MQ=255) tCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAg > 2:5853613/1‑76 (MQ=255) tCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAg < 2:6884104/76‑1 (MQ=255) cGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGc > 2:5419575/1‑76 (MQ=255) cGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGc < 1:140096/76‑1 (MQ=255) gaagaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGcc > 1:6168326/1‑76 (MQ=255) agaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGt < 1:1980640/76‑1 (MQ=255) gaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTa < 2:5850690/76‑1 (MQ=255) gaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTa > 2:2508227/1‑76 (MQ=255) gaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTa > 1:728077/1‑76 (MQ=255) aaaTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTAt > 2:1239236/1‑76 (MQ=255) aaaTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTAt > 1:7297903/1‑76 (MQ=255) aaTGCAGCTAAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATc < 2:5548061/76‑1 (MQ=255) aaTGCAGCTAAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGACCCTCGAAGCGGGTCTGTTCAGCCGTATc < 2:1737856/76‑1 (MQ=255) aTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATcc < 1:984127/76‑1 (MQ=255) tGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCg > 1:13064/1‑76 (MQ=255) tGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCg > 1:4765535/1‑76 (MQ=255) gCAGCTAAAACAGGCGAAGAAAGCCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCgt < 1:5899574/76‑1 (MQ=255) aGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgc > 1:1416193/1‑76 (MQ=255) gCTAAAACGGGCGAAAAAAGCCCTGTCTGAAAAACTGCAGAGCCTCAAAGCGGGTCTTTTCAGCCGTATCCgtgct < 2:4830456/76‑1 (MQ=255) cTCAAACGGGCGAAGAAAGCCCTGTCGAAAAAACTGCAGGCCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctg < 2:7313817/76‑1 (MQ=255) tCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgt > 2:3397909/1‑76 (MQ=255) cAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtg > 2:6136270/1‑76 (MQ=255) cAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtg > 2:408915/1‑76 (MQ=255) aaaaCGGGCGAAGAAAGCCCTGTCTGAAGAACGGCAGACCCTCGAAGGGGGTCTGTCCAGCCGTATCCgtgctgtg < 2:6404118/75‑1 (MQ=255) aaCAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtgct > 2:5742357/1‑76 (MQ=255) aaCAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtgct < 1:2401476/76‑1 (MQ=255) aaCAGGCGAAAAAAGCCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtgct < 1:959026/76‑1 (MQ=255) cAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTgg > 1:3245863/1‑76 (MQ=255) aGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGt < 2:1820715/76‑1 (MQ=255) aGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGt > 2:6110185/1‑76 (MQ=255) ggCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTa < 1:1791967/76‑1 (MQ=255) ggCGAAAAAAGACCTGTCAGAAGACCTGCAGGGCCGCAAAGGGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTa < 2:5057954/76‑1 (MQ=255) gCGAAGAAAGCCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAg < 1:2692499/76‑1 (MQ=255) cGAAGAAAGCCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGc < 1:1302441/76‑1 (MQ=255) gaagaaAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGcc > 1:2841647/1‑76 (MQ=255) gaagaaAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGcc > 1:1023504/1‑76 (MQ=255) aagaaagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCg > 2:7118870/1‑76 (MQ=255) aagaaagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCg > 2:5299303/1‑76 (MQ=255) gaaagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGt > 1:2002185/1‑76 (MQ=255) aaagcCCTGTCTAAAGACCTGCGGGCCCTCGAGGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTg < 1:2508227/76‑1 (MQ=255) aagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTgg > 2:3604642/1‑76 (MQ=255) aagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTCTTCAGCCGTATCCGTGCTGTGCTGGCAGCcggcgg > 1:4846805/1‑76 (MQ=255) agcccTGTCGAAAGAACGGCAGACCCGCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGc < 1:6857787/73‑1 (MQ=255) agaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGc > 1:3911934/1‑76 (MQ=255) agaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGc > 1:4545425/1‑76 (MQ=255) gaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCg > 2:5101140/1‑76 (MQ=255) gaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCg > 1:6687910/1‑76 (MQ=255) cTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTg > 1:6543104/1‑76 (MQ=255) cTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTg > 1:1700024/1‑76 (MQ=255) cTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGAAGCCGGGGGCGTTg > 1:1459140/1‑76 (MQ=255) tGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGa > 1:6946347/1‑76 (MQ=255) tGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGCTGa > 2:2382498/1‑76 (MQ=255) gTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGGTGaa > 2:390536/1‑76 (MQ=255) cTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGc > 2:5109185/1‑76 (MQ=255) cTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGGTGAAGc > 1:5873877/1‑76 (MQ=255) tGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCt > 2:3479624/1‑76 (MQ=255) tGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCt > 2:6850014/1‑76 (MQ=255) tGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGCTGAAGCt > 2:6523223/1‑76 (MQ=255) tGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCAGGTGGCGTTGCAGCt > 1:4047637/1‑76 (MQ=255) gaagaaCTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTg > 2:7200464/1‑76 (MQ=255) aaagatCCGCAGAGCCTCGAGGGGGTTCTTTCCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTg < 2:3824902/75‑1 (MQ=255) agaaCTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTgag > 2:1258275/1‑76 (MQ=255) agaaCCGCAGCGCCGCAAAGCGGGTCTTTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTgag < 1:1651976/76‑1 (MQ=255) gaaCTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTgaga > 2:6939272/1‑76 (MQ=255) aCTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAg > 1:110966/1‑76 (MQ=255) cTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGc > 2:2852790/1‑76 (MQ=255) cAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCg > 1:5166123/1‑76 (MQ=255) cAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCg > 1:3789282/1‑76 (MQ=255) cAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCg > 1:2223444/1‑76 (MQ=255) agagCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGa > 2:2006670/1‑76 (MQ=255) agagCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGa > 1:6015983/1‑76 (MQ=255) agagCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGa > 2:962025/1‑76 (MQ=255) | GACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGA > REL606/4163679‑4163824 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |