| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,273,299 | A→T | intergenic (+227/‑115) | nrfG → / → gltP | heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG/glutamate/aspartate:proton symporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,273,299 | 0 | A | T | 98.1% | 1107.5 / 7.1 | 267 | intergenic (+227/‑115) | nrfG/gltP | heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG/glutamate/aspartate:proton symporter |
| Reads supporting (aligned to +/- strand): ref base A (3/2); new base T (142/120); total (145/122) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
Only 100 of 267 total aligned reads displayed.
CGTTATTATGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCG > REL606/4273226‑4273371 | cgTTATTATGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTc < 2:6474698/76‑1 (MQ=255) tattatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATa > 1:2536908/1‑76 (MQ=255) attatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATac > 2:1573460/1‑75 (MQ=255) attatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATaa > 1:3529461/1‑76 (MQ=255) ttatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAg < 2:5442525/76‑1 (MQ=255) ttatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAg < 2:4671368/76‑1 (MQ=255) ttatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAg > 2:3968280/1‑76 (MQ=255) ttatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAg < 1:5394696/76‑1 (MQ=255) ttatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAg < 1:2755129/76‑1 (MQ=255) tatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGc < 2:6316155/76‑1 (MQ=255) gCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGtt > 1:1038385/1‑76 (MQ=255) gCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGtt < 2:5572891/76‑1 (MQ=255) aTTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTaa > 1:2588692/1‑76 (MQ=255) ttAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTaaa < 1:5703778/76‑1 (MQ=255) ttAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTaaa > 1:1673538/1‑76 (MQ=255) ttAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTaaa < 1:1350269/76‑1 (MQ=255) tAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTaaaa < 2:1333649/76‑1 (MQ=255) tAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTaaaa > 1:370379/1‑76 (MQ=255) aaTGCGGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTaaaaa < 1:2027884/76‑1 (MQ=255) aaTGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTaaaaa > 2:5979755/1‑76 (MQ=255) aaTGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTaaaaa > 2:5374520/1‑76 (MQ=255) aaTGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTaaaaa > 2:3598254/1‑76 (MQ=255) aaTGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTaaaaa > 1:3467496/1‑76 (MQ=255) aaTGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTaaaaa < 2:2138788/76‑1 (MQ=255) aTGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTaaaaaa < 2:2349592/76‑1 (MQ=255) tGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTaaaaaaa > 2:5178797/1‑76 (MQ=255) gCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAAt > 2:2879351/1‑76 (MQ=255) cAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATc < 1:3144341/76‑1 (MQ=255) aGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCt < 2:6705442/76‑1 (MQ=255) cGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTAc < 1:7630764/76‑1 (MQ=255) gAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACa > 1:1531840/1‑76 (MQ=255) gAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACa < 1:6131688/76‑1 (MQ=255) aaaaGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACaa > 2:480969/1‑76 (MQ=255) aaaaGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACaa > 2:5073743/1‑76 (MQ=255) aaaaGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACaa < 1:2881829/76‑1 (MQ=255) aaaGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACaaa < 1:5730075/76‑1 (MQ=255) aaaGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACAGTTCATAAGCGTTAAAAAAATCTACaaa > 2:3326035/1‑76 (MQ=255) aGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAAcc > 1:4592499/1‑76 (MQ=255) aGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAAcc < 1:7469232/76‑1 (MQ=255) gCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCa < 2:5815453/76‑1 (MQ=255) tctGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAAc > 1:5877760/1‑76 (MQ=255) tctGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAAc > 2:7071399/1‑76 (MQ=255) ctGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACg > 1:4677070/1‑76 (MQ=255) gttgttAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCa > 2:4242881/1‑76 (MQ=255) gttgttAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCa < 1:4966099/76‑1 (MQ=255) gttgttAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCa > 2:6135822/1‑76 (MQ=255) ttgttAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCaa < 1:7232541/76‑1 (MQ=255) ttgttAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCaa > 1:7667772/1‑76 (MQ=255) gttAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAaca < 1:1545226/76‑1 (MQ=255) ttAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAacac > 1:446874/1‑76 (MQ=255) tAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAAcaca < 1:2169708/76‑1 (MQ=255) tAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAAcaca < 1:3342592/76‑1 (MQ=255) tAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAAAGCAAcaca > 2:6991262/1‑76 (MQ=255) aaaGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACaa > 2:6274097/1‑76 (MQ=255) aaaGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACaa > 1:4146363/1‑76 (MQ=255) aaaGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACaa > 2:1697302/1‑76 (MQ=255) aaaGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACaa > 2:7425203/1‑76 (MQ=255) aaGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAAt > 1:5176210/1‑76 (MQ=255) aaGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAAt > 2:6015103/1‑76 (MQ=255) aaGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAAt < 2:5100118/76‑1 (MQ=255) gggtGCGCAACATACCGCGCACATGATACTGTTGATAAGCGTTAAAAAAATATAGTCACCAACGTACCACACTTCa > 2:708514/4‑76 (MQ=255) ggTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCa > 1:1298779/1‑76 (MQ=255) ggTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCa > 1:3429880/1‑76 (MQ=255) ggTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCa > 1:3874633/1‑76 (MQ=255) ggTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCa < 1:6589134/76‑1 (MQ=255) ggtGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCAt < 2:1934797/74‑1 (MQ=255) gTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCAt < 2:815165/76‑1 (MQ=255) tGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGc > 2:3653862/1‑76 (MQ=255) gcgcAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGcc > 2:3546306/1‑76 (MQ=255) gcAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCt < 1:7178884/76‑1 (MQ=255) gcAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCt > 2:4532714/1‑76 (MQ=255) gcAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCt > 1:4763371/1‑76 (MQ=255) gcAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCt < 1:1903151/76‑1 (MQ=255) cAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTg < 2:1151681/76‑1 (MQ=255) cAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTg < 1:5541117/76‑1 (MQ=255) cAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTg > 1:3218617/1‑76 (MQ=255) aaCATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTgg < 2:4950356/76‑1 (MQ=255) cATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCa > 2:7564113/1‑76 (MQ=255) cATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCa > 2:7288860/1‑76 (MQ=255) cATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCa > 2:864020/1‑76 (MQ=255) cATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCa > 2:4572754/1‑76 (MQ=255) aTACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAg > 1:84770/1‑76 (MQ=255) aCCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTa > 1:1145404/1‑76 (MQ=255) aCCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTa > 1:2816949/1‑76 (MQ=255) ccGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTAt < 2:5617845/76‑1 (MQ=255) gcgcAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGt < 1:1163540/76‑1 (MQ=255) cgcAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTc < 1:693397/76‑1 (MQ=255) gcAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCa > 2:4824448/1‑76 (MQ=255) aaTGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGt > 1:1453278/1‑76 (MQ=255) aTGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGtt < 1:623272/76‑1 (MQ=255) aTGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGtt < 1:3669492/76‑1 (MQ=255) gATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTtct > 2:1222954/1‑76 (MQ=255) gATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTtct > 1:6621163/1‑76 (MQ=255) gATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTtct > 1:4276357/1‑76 (MQ=255) aTACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTtctc > 1:6882804/1‑76 (MQ=255) aTACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTtctc > 2:5505766/1‑76 (MQ=255) tACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTcg < 2:6907588/76‑1 (MQ=255) tACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTcg > 2:2636840/1‑76 (MQ=255) tACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTcg > 2:4196410/1‑76 (MQ=255) aCTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTcgc > 1:6506795/1‑76 (MQ=255) | CGTTATTATGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCG > REL606/4273226‑4273371 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |