BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	650,854	T→C	N149S (AAC→AGC)	ybeA ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	650,854	0	T	C	100.0%	748.5 / NA	180	N149S (AAC→AGC)	ybeA	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (96/84); total (96/84)

Only 100 of 203 total aligned reads displayed.

AAAAGAGTTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGTTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCACGCGAACCAGCGGATGGGGGAGGGTAA  >  REL606/650781‑650926

 aaaGAGTTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGAtggctgg                                                                       >  2:4352091/1‑76 (MQ=255)

  aaGAGTTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGAtggctggt                                                                      >  1:4688074/1‑76 (MQ=255)

   atagTTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCtggtg                                                                     <  2:6553488/74‑1 (MQ=255)

     agTTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTggtggt                                                                   <  2:3246502/76‑1 (MQ=255)

      gTTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGgtggtg                                                                  <  2:2643887/76‑1 (MQ=255)

      gTTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGgtggtg                                                                  <  1:3735674/76‑1 (MQ=255)

       ttCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGa                                                                 >  2:4360300/1‑76 (MQ=255)

        tCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGAt                                                                <  1:7228344/76‑1 (MQ=255)

        tCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGAt                                                                >  1:476877/1‑76 (MQ=255)

        tCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGAt                                                                <  1:4015382/76‑1 (MQ=255)

        tCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACCCACGGTGATAAGGATGGCTGGTGGTGAt                                                                <  1:1475004/76‑1 (MQ=255)

         cTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATg                                                               <  1:6368138/76‑1 (MQ=255)

         cTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATg                                                               >  2:4703801/1‑76 (MQ=255)

         cTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGGGATg                                                               >  2:743618/1‑76 (MQ=255)

            tAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTc                                                            <  2:3304593/76‑1 (MQ=255)

             aGTTTCATCCGCTGCGTTTTCTAGTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTcc                                                           >  1:7400214/1‑76 (MQ=255)

             aGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTcc                                                           >  1:3416255/1‑76 (MQ=255)

             aGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTcc                                                           <  1:582466/76‑1 (MQ=255)

             aGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGGGGTGCtgc                                                           >  1:7013525/1‑74 (MQ=255)

              gTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCa                                                          >  2:257779/1‑76 (MQ=255)

              gTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGGGGTGATGGTCCa                                                          >  1:5924381/1‑76 (MQ=255)

               tttCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGGTCCAc                                                         >  1:2712926/1‑76 (MQ=255)

               tttCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCAc                                                         <  2:3923315/76‑1 (MQ=255)

                ttCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACg                                                        <  2:3652787/76‑1 (MQ=255)

                ttCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACg                                                        <  2:6239162/76‑1 (MQ=255)

                 tCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGc                                                       <  1:2346924/76‑1 (MQ=255)

                  cATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGcc                                                      <  2:6902519/76‑1 (MQ=255)

                  cATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGcc                                                      >  1:6741335/1‑76 (MQ=255)

                   aTCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGccc                                                     <  1:3848622/76‑1 (MQ=255)

                    tCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCg                                                    <  1:5518336/76‑1 (MQ=255)

                     ccGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCgg                                                   >  1:902111/1‑76 (MQ=255)

                      cGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGt                                                  >  2:4532724/1‑76 (MQ=255)

                      cGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGt                                                  <  1:6492261/76‑1 (MQ=255)

                      cGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGt                                                  <  1:1536970/76‑1 (MQ=255)

                      cGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGt                                                  <  2:5512515/76‑1 (MQ=255)

                      cGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGt                                                  <  1:3148827/76‑1 (MQ=255)

                       gCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTa                                                 <  2:852718/76‑1 (MQ=255)

                       gCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGGGATGCTCCACGCCCggca                                                 >  1:5571756/1‑74 (MQ=255)

                        cTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTAc                                                <  2:4175927/76‑1 (MQ=255)

                        cTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTAc                                                >  1:1280714/1‑76 (MQ=255)

                         tGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACa                                               <  1:1152007/76‑1 (MQ=255)

                          gCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAg                                              <  2:330894/76‑1 (MQ=255)

                            gTTTTCTACTCAAAGCTCCCTTATCACTCACGGGGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGAc                                            <  2:2303111/76‑1 (MQ=255)

                                tCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGActctc                                        <  2:927461/76‑1 (MQ=255)

                                tCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGActctc                                        >  2:2855093/1‑76 (MQ=255)

                                 cTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGActctct                                       <  1:3890534/76‑1 (MQ=255)

                                 cTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGActctct                                       >  2:50206/1‑76 (MQ=255)

                                 cTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGActctct                                       >  2:3054978/1‑76 (MQ=255)

                                  tACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTg                                      <  1:1684450/76‑1 (MQ=255)

                                   aCTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGc                                     >  1:3455937/1‑76 (MQ=255)

                                   aCTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGc                                     >  1:281478/1‑76 (MQ=255)

                                    cTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACCGACTCTCTGCg                                    >  2:2163835/1‑76 (MQ=255)

                                    cTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCg                                    <  2:492957/76‑1 (MQ=255)

                                       aaaGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGAcc                                 >  2:7059986/1‑76 (MQ=255)

                                        aaGCTCCCTTATCACTCACGGTGATAAGGATGGCTGGAGGATATGCTCCACGCCCGGTACAGACCCTCGGCGATCa                                >  2:5591661/1‑76 (MQ=255)

                                        aaGCTCACTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCTCGCCCGGTACAGACTCTCTGCGACCa                                >  2:7479152/1‑76 (MQ=255)

                                          gCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGc                              <  2:2382955/76‑1 (MQ=255)

                                          gCTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGc                              <  2:4313209/76‑1 (MQ=255)

                                           cTCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCa                             >  1:7500812/1‑76 (MQ=255)

                                            tCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCAc                            >  2:5810764/1‑76 (MQ=255)

                                            tCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCAc                            >  1:1881729/1‑76 (MQ=255)

                                            tCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCAc                            <  2:7280856/76‑1 (MQ=255)

                                            tCCCTTATCACTCACGGTGATAAGGATGGCTGGTGGGGATGCTCCACCCCCGGTACAGAATCTCTGCGACCAGCAc                            >  1:321338/1‑76 (MQ=255)

                                             cccTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCAcg                           >  2:4242557/1‑76 (MQ=255)

                                              ccTTATCACTCACGGTGATAAGGATGGCTGGGGGGGATGCTCCACGCCCGGGACAGACTCGCTGCGACCAACAcgc                          >  2:3879112/1‑76 (MQ=255)

                                               cTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGGAcgcg                         >  1:202621/1‑76 (MQ=255)

                                               cTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCAcgcg                         <  2:7191779/76‑1 (MQ=255)

                                               cTTATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCAcgcg                         >  1:877322/1‑76 (MQ=255)

                                               cTTATCACTCACGGTGATAAGGATGGCTGGTGGGGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCAcgcg                         >  2:4435403/1‑76 (MQ=255)

                                                ttATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACCGACTCTCTGCGACCAGCAAGCGa                        >  2:4738457/1‑76 (MQ=255)

                                                 tATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCACGCGaa                       >  1:1090433/1‑76 (MQ=255)

                                                 tATCACTCACGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCACGCGaa                       >  1:5653086/1‑76 (MQ=255)

                                                 tATCACTCACGGTGATAAGGATGGCTGGTGGGGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCACGCGaa                       >  1:967123/1‑76 (MQ=255)

                                                   tcactcacGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCACGCGAAcc                     <  1:7319738/76‑1 (MQ=255)

                                                     actcacGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCACGCGAACCAg                   <  2:156520/76‑1 (MQ=255)

                                                      ctcacGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCACGCGAACCAGc                  <  1:6775068/76‑1 (MQ=255)

                                                      ctcacGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCACGCGAACCAGc                  <  2:6665219/76‑1 (MQ=255)

                                                      ctcacGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCACGCGAACCAGc                  <  2:7309146/76‑1 (MQ=255)

                                                       tcacGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCACGCGAACCAGCg                 <  1:1980157/76‑1 (MQ=255)

                                                        cacGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCACGCGAACCAGCgg                >  1:5009187/1‑76 (MQ=255)

                                                          cGGTGATAGGGATGGCTGGTGGTGAGGCTCCACCCCCGGTACAGACTCTCTGCGACCAGCACGCGAACCAGCGGAt              <  1:5173919/76‑1 (MQ=255)

                                                          cGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCACGCGAACCAGCGGAt              <  2:7727150/76‑1 (MQ=255)

                                                          cGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCACGCGAACCAGCGGAt              <  2:5118398/76‑1 (MQ=255)

                                                          cGGTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCACGCGAACCAGCGGAt              <  1:5280277/76‑1 (MQ=255)

                                                           ggTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCACGCGAACCAGCGGATg             <  1:4846564/76‑1 (MQ=255)

                                                           ggTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCACGCGAACCAGCGGATg             <  2:5965105/76‑1 (MQ=255)

                                                            gTGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCACGCGAACCAGCGGATgg            <  2:1896291/76‑1 (MQ=255)

                                                             tGATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCACGCGAACCAGCGGATggg           >  1:4660815/1‑76 (MQ=255)

                                                              gATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCACGCGAACCAGCGGATgggg          <  2:1049653/76‑1 (MQ=255)

                                                              gATAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCACGCGAACCAGCGGATgggg          >  1:2568226/1‑76 (MQ=255)

                                                               aTAAGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCACGCGAACCAGCGGATggggg         >  1:7049471/1‑76 (MQ=255)

                                                                 aaGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCACGCGAACCAGCGGATggggggg       >  1:5387884/1‑74 (MQ=255)

                                                                 aaGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCACGCGAACCAGCGGATGGGGGAg       >  2:5915802/1‑76 (MQ=255)

                                                                 aaGGATGGCTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCACGCGAACCAGCGGATGGGGGAg       >  1:5422812/1‑76 (MQ=255)

                                                                  agtaTGGCTGGTGGTGATGCCCCCCGCCCGGTACAGACTCTCTGCTACCAGCACGCGAACCAGCGGATGGGGGAgg      <  2:2263062/73‑1 (MQ=255)

                                                                  aGGATGGCTGGGGGGGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCACGCGAACCAGCGGATgggggggg      >  2:1123031/1‑76 (MQ=255)

                                                                    gATGGCTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCACGCGAACCAGCGGATGGGGGAGGGt    <  1:7377339/76‑1 (MQ=255)

                                                                     aTGGCTGGTGGGGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCACGCGGACCAACGGAAGGGGGAGGGTa   >  1:7724349/1‑76 (MQ=255)

                                                                      tggctgttGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCACGCGAACCAGCGGATGGGGGAGGGTaa  <  1:2168961/76‑1 (MQ=255)

                                                                      tggctggtGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCACGCGAACCAGCGGATGGGGGAGGGTaa  <  1:7632992/76‑1 (MQ=255)

AAAAGAGTTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGTTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCACGCGAACCAGCGGATGGGGGAGGGTAA  >  REL606/650781‑650926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`