Predicted mutation
evidence seq id position mutation annotation gene description
RA REL606 2,193,779 G→T L143I (CTT→ATT)  yeiB ← conserved inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6062,193,7790GT100.0% 658.8 / NA 176L143I (CTT→ATT) yeiBconserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (59/117);  total (59/117)

Only 100 of 201 total aligned reads displayed.

CCGATGCATCCGGCGTCCAGGCGCGGCTGG‑TCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193707‑2193851
                                                                          |                                                                        
 cGATGCATCCGGCGTCCAGGCGCGGCTGG‑GCTGGCTGGCGGGAATCAGCCCCCATAACACGCAAAA‑GCCAAAcccc                                                                      >  1:2670682/1‑76 (MQ=255)
  gATGCATCCGGCGTCCAGGCGCGGCTGG‑TCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCcca                                                                     <  2:7704498/76‑1 (MQ=255)
  gATGCATCCGGCGTCCAGGCGCGGCTGG‑TCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCcca                                                                     <  1:3271168/76‑1 (MQ=255)
  gATGCATCCGGCGTCCAGGCGCGGCTGG‑GCTGGCTGTCGGGAAACAGCCCCCATAACA‑GCAAAACGCCAAAccccc                                                                     >  1:2858951/1‑75 (MQ=255)
   aTGCATCCGGCGTCCAGGCGCGGCTGG‑TCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCccac                                                                    <  1:2390715/76‑1 (MQ=255)
     gCATCCGGCGTCCAGGCGCGGCTGG‑TCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCccacca                                                                  <  1:2940265/76‑1 (MQ=255)
      cATCCGGGGTCCAGGCGCGGCTGG‑TCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAg                                                                 <  2:1112332/76‑1 (MQ=255)
      cATCCGGCGTCCAGGCGCGGCTGG‑GCTGGCTGTCGGGAAACAGCCCCCATAAAA‑GCAAAACGCCCATCcccccccg                                                                 >  1:180841/1‑74 (MQ=255)
      cATCCGGCGTCCAGGCGCGGCTGG‑GCTGGCTGGCGGGAAACAGCCCCCATAACA‑GCCAAAAGCCCATACCCCCCAg                                                                 >  2:4488016/1‑76 (MQ=255)
       aTCCGGCGTCCAGGCGCGGCTGG‑TCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGa                                                                <  2:6350049/76‑1 (MQ=255)
        tCCGGCGTCCAGGCGCGGCTGG‑TCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGAt                                                               <  2:2260048/76‑1 (MQ=255)
        tCCGGCGTCCAGGCGCGGCTGG‑TCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCACCACCAGAt                                                               <  1:4590316/76‑1 (MQ=255)
         ccGGCGTCCAGGCGCGGCTGGTTCTGCCGTCCGGAATTCAGCCCC‑CTAAAA‑ACAAAAAGCACAAACCCACCAGaca                                                              >  2:5884897/1‑74 (MQ=255)
         ccGGCGTCCAGGCGCGGCTGG‑TCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATa                                                              <  1:5861048/76‑1 (MQ=255)
          cGGCGTCCAGGCGCGGCTTG‑TCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATaa                                                             <  2:5417492/76‑1 (MQ=255)
          cGGCGTCCAGGCGCGGCTGG‑TCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATaa                                                             <  1:3957955/76‑1 (MQ=255)
          cGGCGTCCAGGCGCGGCTGG‑TCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATaa                                                             <  2:7033114/76‑1 (MQ=255)
           ggCGTCCAGGCGCGGCTGG‑TCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATaaa                                                            <  1:4524554/76‑1 (MQ=255)
           ggCGTCCAGGCGCGGCTGG‑GGTGGCTTTCGGAAAATAACCCCCAAAACA‑GCAAAACGCCCATACCCACCCGAaaaa                                                            >  1:2091740/1‑76 (MQ=255)
            gCGTCCAGGCGCGGCTGG‑TCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAg                                                           <  1:2226/76‑1 (MQ=255)
            gCGTCCAGGCGCGGCTGG‑TCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAg                                                           <  1:5993360/76‑1 (MQ=255)
            gCGTCCAGGCGCGGCTGG‑TCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAg                                                           <  1:6671189/76‑1 (MQ=255)
            gCGTCCAGGCGCGGCTGG‑TCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAg                                                           <  2:4854478/76‑1 (MQ=255)
             cGTCCAGGCGCGGCTGG‑TCTGGCTGTCGTAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGc                                                          <  1:1640016/76‑1 (MQ=255)
             cGTCCAGGCGCGGCTGG‑TCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGc                                                          <  1:7692679/76‑1 (MQ=255)
               tCCAGGCGCGGCTGG‑TCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCAt                                                        <  1:2432967/76‑1 (MQ=255)
               tCCAGGCGCGGCTGG‑TCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCAt                                                        <  2:4956491/76‑1 (MQ=255)
               tCCAGGCGCGGCTGG‑TCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCAt                                                        <  2:444735/76‑1 (MQ=255)
                 cAGGCGCGGCTGG‑TCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGa                                                      <  1:2587348/76‑1 (MQ=255)
                  aGGCGCGGCTGG‑TCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGAc                                                     <  2:7245129/76‑1 (MQ=255)
                  aGGCGCGGCTGG‑GCTGGCTGGCGGGAAACAGCCCCAAAAACA‑GCAAAAACCCAAACCCCACCCGATAAAACATGaa                                                     >  2:3944720/1‑75 (MQ=255)
                    gcgcGGCTGG‑TCTGGCTGGCGGAAATCAGGCCCAATAACA‑GCAAAACGCTAATCCCCACCAGATAACGCATGAAGc                                                   >  2:5264161/1‑76 (MQ=255)
                         gCTGG‑TCTGGCTGTCGGAAATCCGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTa                                              <  1:5060252/76‑1 (MQ=255)
                         gCTGG‑TCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTa                                              >  1:4803324/1‑76 (MQ=255)
                           tGG‑TCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            >  1:4410371/1‑76 (MQ=255)
                           tGG‑TCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            >  1:5184439/1‑76 (MQ=255)
                           tGG‑TCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            >  1:5317288/1‑76 (MQ=255)
                           tGG‑TCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            <  2:5380240/76‑1 (MQ=255)
                           tGG‑TCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            >  1:5921901/1‑76 (MQ=255)
                            gg‑TCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                           >  2:6546130/1‑76 (MQ=255)
                            gg‑TCAGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                           <  1:7280058/76‑1 (MQ=255)
                             g‑TCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa                                          <  1:3733014/76‑1 (MQ=255)
                             g‑TCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa                                          <  2:3177997/76‑1 (MQ=255)
                             g‑TCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa                                          <  1:7071393/76‑1 (MQ=255)
                             g‑TCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa                                          <  1:7403073/76‑1 (MQ=255)
                              gccTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCGCCAGATAAAGCATGACGCCTGTATTaa                                          <  1:1072984/74‑1 (MQ=255)
                               tCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa                                         <  1:1525545/76‑1 (MQ=255)
                                cTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                        <  1:186111/76‑1 (MQ=255)
                                 tGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa                                       >  2:7422068/1‑76 (MQ=255)
                                 tGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa                                       >  1:6810964/1‑76 (MQ=255)
                                     tGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt                                   <  2:734272/76‑1 (MQ=255)
                                      gTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  <  2:5157115/76‑1 (MQ=255)
                                       tCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt                                 <  2:4156649/76‑1 (MQ=255)
                                        cGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                                >  1:5422333/1‑76 (MQ=255)
                                        cGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                                >  2:1635273/1‑76 (MQ=255)
                                         ggAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                               >  1:962561/1‑76 (MQ=255)
                                          gAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                              <  2:2382397/76‑1 (MQ=255)
                                          gAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                              >  1:6516161/1‑76 (MQ=255)
                                          gAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                              <  2:155953/76‑1 (MQ=255)
                                           aaaTCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                             >  2:1602134/1‑76 (MQ=255)
                                            aaTCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAcc                            <  2:2138962/76‑1 (MQ=255)
                                             aTCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg                           >  1:1015178/1‑76 (MQ=255)
                                             aTCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg                           <  1:3903800/76‑1 (MQ=255)
                                             aTCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg                           <  2:6434289/76‑1 (MQ=255)
                                             aTCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg                           >  1:3681062/1‑76 (MQ=255)
                                             aTCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg                           <  1:1476275/76‑1 (MQ=255)
                                             aTCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg                           <  1:1736249/76‑1 (MQ=255)
                                             aTCAGCCACAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg                           <  2:720286/76‑1 (MQ=255)
                                              tCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGa                          <  2:676621/76‑1 (MQ=255)
                                              tCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGa                          <  1:330715/76‑1 (MQ=255)
                                               cAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt                         <  2:2802918/76‑1 (MQ=255)
                                               cAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt                         >  1:5747263/1‑76 (MQ=255)
                                               cAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt                         >  2:5524302/1‑76 (MQ=255)
                                                aGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAACGATg                        >  1:4237585/1‑76 (MQ=255)
                                                  ccccAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc                      <  2:6255428/76‑1 (MQ=255)
                                                  ccccAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc                      <  1:6157792/76‑1 (MQ=255)
                                                  ccccAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc                      >  2:1732338/1‑76 (MQ=255)
                                                    ccAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcgc                    >  2:4307957/1‑76 (MQ=255)
                                                    ccAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcgc                    >  2:4543147/1‑76 (MQ=255)
                                                    ccAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcgc                    >  2:5979339/1‑76 (MQ=255)
                                                      aaTAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCAt                  >  1:361697/1‑76 (MQ=255)
                                                        tAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg                >  2:2707595/1‑76 (MQ=255)
                                                        tAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg                <  1:4288988/76‑1 (MQ=255)
                                                        tAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg                <  2:1208248/76‑1 (MQ=255)
                                                        tAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg                <  1:1300147/76‑1 (MQ=255)
                                                          aCA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgcg              >  2:3386294/1‑76 (MQ=255)
                                                            a‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCa            <  1:4825604/76‑1 (MQ=255)
                                                              gCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAc           >  1:4980599/1‑76 (MQ=255)
                                                               cAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAcc          >  2:2961993/1‑76 (MQ=255)
                                                                aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         >  2:5436629/1‑76 (MQ=255)
                                                                aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         <  1:306369/76‑1 (MQ=255)
                                                                aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         <  1:341690/76‑1 (MQ=255)
                                                                aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         <  1:4666817/76‑1 (MQ=255)
                                                                aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         <  2:1091009/76‑1 (MQ=255)
                                                                   aCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgcc      >  2:4463477/1‑76 (MQ=255)
                                                                      ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc   >  2:6116725/1‑76 (MQ=255)
                                                                      ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc   <  1:6392576/76‑1 (MQ=255)
                                                                       cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  >  2:3761343/1‑76 (MQ=255)
                                                                       cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  >  2:2529083/1‑76 (MQ=255)
                                                                       cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  >  1:549121/1‑76 (MQ=255)
                                                                          |                                                                        
CCGATGCATCCGGCGTCCAGGCGCGGCTGG‑TCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193707‑2193851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑