| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 475,161 | C→T | G49S (GGC→AGC) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 475,161 | 0 | C | T | 99.3% | 498.9 / NA | 137 | G49S (GGC→AGC) | ybaL | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base C (0/1); major base T (82/53); minor base G (0/1); total (82/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.97e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
Only 100 of 182 total aligned reads displayed.
CCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGC > REL606/475088‑475233 | ccGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCg > 1:3527318/1‑76 (MQ=255) cGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCgg < 2:2678737/76‑1 (MQ=255) aCGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAg < 2:709516/76‑1 (MQ=255) aCGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAg < 1:2368774/76‑1 (MQ=255) cGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGt < 2:2978331/76‑1 (MQ=255) cGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGt > 2:3684199/1‑76 (MQ=255) cGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGt > 2:3468954/1‑76 (MQ=255) gCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTg > 1:4823615/1‑76 (MQ=255) ccAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGa > 1:3877982/1‑76 (MQ=255) ccAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGa < 1:3892233/76‑1 (MQ=255) aaaCATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAAt > 1:5550055/1‑76 (MQ=255) aaaCATCAACAGAATAACGCCCAGTCCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAAt < 1:5421926/76‑1 (MQ=255) aCATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATgg < 1:1552190/76‑1 (MQ=255) cATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGt > 1:1576533/1‑76 (MQ=255) cATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGt > 1:4155032/1‑76 (MQ=255) cATCAACAGAACGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGt > 1:498056/1‑76 (MQ=255) aTCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTc < 1:2717508/76‑1 (MQ=255) cAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCt < 1:853726/76‑1 (MQ=255) cAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGGCCt > 2:4037958/1‑76 (MQ=255) aaCAGAATGACGCCCAGTGCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTg < 2:4984163/76‑1 (MQ=255) aCAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGc < 1:894012/76‑1 (MQ=255) cAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGGATGGTCCTGcc > 2:2478155/1‑76 (MQ=255) gAATAACACCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAg < 2:164268/76‑1 (MQ=255) gAAGGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAg < 1:3939110/76‑1 (MQ=255) gACGCCCGGTGCACCCGGTCCCGGGACAAGCTTTGTATCGGCAAAAAAGCTCGGAGTGAATGGTCCTGCCAGcaca < 1:2616601/76‑1 (MQ=255) gACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCCGcaca > 1:3988804/1‑76 (MQ=255) gACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGccca > 2:618457/1‑76 (MQ=255) gACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGGCCTGCCAGcacc > 2:3393859/1‑75 (MQ=255) aCGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCccac > 2:5000732/1‑76 (MQ=255) gCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACAccc > 1:2359311/1‑76 (MQ=255) ccAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGc > 2:3124122/1‑76 (MQ=255) cAGTTCAGCCAGTTCGGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCt < 1:2629029/76‑1 (MQ=255) cAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCt < 1:175556/76‑1 (MQ=255) cAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCt > 1:1068777/1‑76 (MQ=255) cAGTCCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCt < 1:2475925/76‑1 (MQ=255) ttCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTaaa > 2:4197167/1‑75 (MQ=255) ttCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAAc < 1:868920/76‑1 (MQ=255) ttCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAAc > 2:1411318/1‑76 (MQ=255) aGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGa < 1:4818610/76‑1 (MQ=255) aGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGGCCTGCCAGCACACCCGCTAACAGa > 2:1314266/1‑76 (MQ=255) gCCGGTTCCGGGGCAAGCTTGGTAGCGGCAACAAAGCGCGGAGTGAAGGGCCCTGCCAGCACACCCGCTAACAGat < 2:109670/76‑1 (MQ=255) gCCAGTCCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCTAGCACCCCCGCTAACAGat < 2:6527620/76‑1 (MQ=255) gCCAGTCCCGGGCAAAGTTTGGTATCGGCACAAAAGCTCGGAGTGAATGGCCCTGCCAGCACACCCGCTAACAGat < 2:2023204/76‑1 (MQ=255) ccAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGata < 2:1597512/76‑1 (MQ=255) cAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACCCCCGCTGACAGatct > 2:5215539/1‑74 (MQ=255) cAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGctat > 2:6396496/1‑76 (MQ=255) cAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGatat < 1:4384618/76‑1 (MQ=255) aGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATc > 1:5304705/1‑76 (MQ=255) aGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATc < 1:1572454/76‑1 (MQ=255) aGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAAATATc > 1:739413/1‑76 (MQ=255) gTTCCGGGGCAAGTTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATcc < 1:5080213/76‑1 (MQ=255) ttCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATccc > 2:816788/1‑76 (MQ=255) tcccGGGGCAAGCTGTGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATccc < 1:1923648/74‑1 (MQ=255) tCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCcca > 2:4468800/1‑76 (MQ=255) tCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCcca < 1:3400469/76‑1 (MQ=255) cgggggAAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAAGGGCCCTGCCAGCACACCCGCTAACAGATATCccac < 2:1991485/74‑1 (MQ=255) ccGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAAAAGATATCccac > 1:4200697/1‑76 (MQ=255) ccGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGGCCTGCCAGCACACCCCCTAACAGATATCccaa > 1:1178861/1‑75 (MQ=255) ccGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGAGAATGGTCCTGCCAGCACACCCCCTAACAAATATCccac > 1:3034093/1‑76 (MQ=255) ccGGGGCAAGCTAGGTACTGGAAACAAAGCTCGGAGTAAATGGTCCTGCCCGCACACCCGCTAACAGATATCccac < 2:2100824/76‑1 (MQ=255) ggggCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCccacca > 1:6514004/1‑76 (MQ=255) gggCAAGTTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCag < 1:2627943/76‑1 (MQ=255) gggCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCag > 2:2317438/1‑76 (MQ=255) gggCAAGCTGGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCag < 2:4823615/76‑1 (MQ=255) gCAAGTTTGGTACCGGCAAAAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCagag < 1:2545457/76‑1 (MQ=255) cAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAgagg > 1:5873052/1‑76 (MQ=255) aaGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAgagga > 1:2255788/1‑76 (MQ=255) aaGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATAACCCACCAgagga > 1:6295669/1‑76 (MQ=255) gtttGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGagaaga < 1:4325899/74‑1 (MQ=255) gCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGgaga > 2:4352024/1‑76 (MQ=255) agttGTTACCGGCAACAAAGCTCGGATTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGgaga < 2:1379171/74‑1 (MQ=255) tttGGTATCAGCAACAAAGCCCGGAGTGAATGGCCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGaa < 2:5753888/75‑1 (MQ=255) ctgggTATGGGCAACAAGGCTCGGAGTAAATGGCCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGaa < 1:5695803/73‑1 (MQ=255) tttgTATCGGCAACAAATCCGGGAGTGAAGGCTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGaaa < 1:4665791/73‑1 (MQ=255) ttGGTATCGGCAACAAAGCTCGGATTAAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGaaa < 1:1096492/76‑1 (MQ=255) ttGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGaaa > 1:5746915/1‑76 (MQ=255) ttGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGaaa < 2:3225235/76‑1 (MQ=255) tGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAAt < 1:2874022/76‑1 (MQ=255) tGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAAt > 1:5655120/1‑76 (MQ=255) tGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAAt > 2:4587157/1‑76 (MQ=255) tGGTATCGGCAACAAAGCTCGGAGTGAATGGCCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAAt < 2:2145568/76‑1 (MQ=255) ggTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATa > 1:2071253/1‑76 (MQ=255) ggTACCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATa < 2:6054166/76‑1 (MQ=255) tATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACg > 1:3257290/1‑76 (MQ=255) tATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCAACCAGAGGAGAAATACg < 1:5474183/76‑1 (MQ=255) aTCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGt > 2:3324235/1‑76 (MQ=255) aTCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGt > 1:315438/1‑76 (MQ=255) aTCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGt > 2:1223375/1‑76 (MQ=255) tCGTCAACAAGGCTGGGGGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTa < 1:4568005/76‑1 (MQ=255) tCGGCAACAAACCCCGGAGTGAATGGCCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTa < 1:4321667/76‑1 (MQ=255) ggCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGt > 2:1602917/1‑76 (MQ=255) caacaaAGCTGGGAGGGAAGGGCCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGttt < 1:1126673/76‑1 (MQ=255) caaAGCTCGGAGTGAATGTCCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTAtt < 1:5183475/76‑1 (MQ=255) caaAGCTCGCAGTGAATGGCCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTAtt < 2:5896251/76‑1 (MQ=255) aaaGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTg > 2:5670676/1‑76 (MQ=255) aaaGCTCGGAGTGAATGGCCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTg < 1:5910463/76‑1 (MQ=255) aaGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTgg < 1:3237763/76‑1 (MQ=255) aaGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTgg > 2:2889124/1‑76 (MQ=255) aaGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTgg < 2:3475093/76‑1 (MQ=255) aGCTCGGAGGGAAGGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGc < 1:3667824/76‑1 (MQ=255) | CCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGC > REL606/475088‑475233 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |