| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,500,743 | G→A | R21C (CGC→TGC) | glgC ← | glucose‑1‑phosphate adenylyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,500,743 | 0 | G | A | 98.8% | 277.6 / NA | 81 | R21C (CGC→TGC) | glgC | glucose‑1‑phosphate adenylyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (28/52); total (28/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 142 total aligned reads displayed.
TTGGTTAAATCCTTCAGGCGGGTACCACGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACA > REL606/3500670‑3500815 | ttGGTTAAATCCTTCAGGCGGGTACCACGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGgggcg > 1:2525497/1‑76 (MQ=255)gtGGTTAAACCCTGGAGGGGGCACCCCCGTCCGCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGgcacg < 2:3155037/75‑1 (MQ=255) tGGTTAAATCCTTCAGGCGGGTACCACGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGgcacg‑c > 1:3195687/1‑76 (MQ=255) tGGTTAAATCCTTCAGGCGGGTACCACGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCGGgcacg‑c > 1:2369832/1‑76 (MQ=255) ggTTAAATCCTTCAGGCGGGTACCACGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGGTGGgccg‑cc > 1:2662415/1‑76 (MQ=255) ttAAATCCTTCAGGCGGGTACCACGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGGGGGGACG‑CCa‑a > 2:534387/1‑76 (MQ=255) tAAATCCTTCAGGCGGGTACCACGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGGTGGGACG‑CCA‑Ac > 2:1327896/1‑76 (MQ=255) tAAATCCTTCAGGCGGGTACCACGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGGCCG‑CCA‑Ac > 2:1148730/1‑76 (MQ=255) aaaTCCTTCAGGCGGGTACCACGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGGTGGGGCG‑GCa‑aaa > 2:181428/1‑74 (MQ=255) aaaTCCTTCAGGCGGGTACCACGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCACG‑CCc‑aca > 1:670434/1‑76 (MQ=255) aaaTCCTTCAGGCGGGTACCACGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCACG‑CCA‑ACa > 1:1449988/1‑76 (MQ=255) aaaCCCTTCGGGGGGGCACCCCCCCCCCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCACG‑CCA‑ACa < 2:1587293/76‑1 (MQ=255) acccTTCGGGCGCGTCCCCCCCCCCCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCACG‑CCA‑ACAtt < 1:3497297/74‑1 (MQ=255) aTCCTTCAGGCGGGTACCACGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCGGGGACG‑CCC‑ACCtt > 2:2112895/1‑76 (MQ=255) aTCCTTCAGGCGGGTACCACGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCGGGCACG‑CCA‑ACaat > 2:4313090/1‑74 (MQ=255) ccTTCAGGCGGGTACCACGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGGTGGCCCG‑CCA‑ACACTaa > 2:6442144/1‑76 (MQ=255) cTTCAGGCGGGTACCACGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCACG‑CCC‑AAACTAAg > 2:4911043/1‑76 (MQ=255) ttCAGGCGGGTACCACGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGGTGGCACG‑CCC‑ACATTaaag > 2:942857/1‑74 (MQ=255) ttCAGGCGGGTACCACGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCACG‑CCA‑ACAATAAga > 1:4667953/1‑75 (MQ=255) cAGGCGGGTACCACGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCACG‑CCA‑AACTTAAGTGa > 2:3096032/1‑76 (MQ=255) aGGCGGGTACCACGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGGTGGGCCG‑CCC‑ACAATTAGTGAt > 2:573453/1‑76 (MQ=255) ggCGGGTACCACGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGGGGGGGCG‑CCACAAATTAAATGac > 2:5251432/1‑75 (MQ=255) cGGGTACCACGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGt > 2:3242968/1‑76 (MQ=255) gggTCCCGCGCCCCCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGtt < 2:1863059/76‑1 (MQ=255) gggTCCCCCCTCCCCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGtt < 2:5910826/76‑1 (MQ=255) gggTACCACGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGtt > 1:715221/1‑76 (MQ=255) gggTACCACGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGtt > 1:1224526/1‑76 (MQ=255) tACCACGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGttctt > 2:3960192/1‑76 (MQ=255) cACGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCACG‑CCC‑ACATTAAGTGAT‑CGTTCTtctc > 2:5147650/1‑76 (MQ=255) cACGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCACG‑CCC‑AAATTAAAGGGT‑CGTTCTtttc > 2:5935255/1‑76 (MQ=255) cGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTa > 2:567577/1‑76 (MQ=255) cctccCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTTTCTAAAc > 1:2557114/1‑76 (MQ=255) ccccccGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAAc < 2:327984/73‑1 (MQ=255) ccccccGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAAc < 2:701293/73‑1 (MQ=255) ctccCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACt > 1:2168826/1‑76 (MQ=255) cccccGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACt < 1:1533200/74‑1 (MQ=255) cccccGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACt < 1:3620293/74‑1 (MQ=255) tccCGCCAGTATCAGGGCAACAGATTTCAATGGCAGGTGGGACGCCCA‑AAATTAA‑TGATCCGTTTTTTTTTAAAca > 2:4246859/1‑75 (MQ=255) tccCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTa > 2:3949963/1‑76 (MQ=255) tccCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCACG‑CCA‑AAATTAAATGAT‑CGTTCTTCTCTAAACTa > 2:287434/1‑76 (MQ=255) cccGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGGCCG‑CCA‑ACATTAAAGGAT‑CGTTCATCTCTCAACTaa > 2:5654295/1‑76 (MQ=255) cccGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGGACG‑CCA‑ACATTAAGTGAT‑CGTTATTCTCTAAAATaa > 1:5046825/1‑76 (MQ=255) cccGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTaa < 1:5635732/76‑1 (MQ=255) cccGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTaa > 1:3294113/1‑76 (MQ=255) ccGCCAGTATCAGGGCAACAGATTTCAATGGCAGGTGGCACG‑CCC‑AAATTAAATGAT‑CGTTCTTCTCTAAACTAAc > 2:4357578/1‑76 (MQ=255) gCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCa < 1:5926048/76‑1 (MQ=255) ccAGTATCAGGGCAACAGATTTCAATGGCAGGTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTTTTAACTAACCAt > 2:6025612/1‑76 (MQ=255) ccAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCAt > 1:2261281/1‑76 (MQ=255) ccAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCAt < 1:4608642/76‑1 (MQ=255) ccAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCAt < 2:3708251/76‑1 (MQ=255) ccAGTATCAGGGCAACAGAGTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCAt < 2:2912513/76‑1 (MQ=255) cAGTATCAGGGCAACAGATTTCAATGGCAGGTGGCGCG‑CCA‑AAATTCAGTGAT‑TGTTCTTCTCCAAACTAACCATg > 2:2096885/1‑76 (MQ=255) cAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATg < 1:3376315/76‑1 (MQ=255) cAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATg < 1:3102424/76‑1 (MQ=255) cAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATg < 1:6029458/76‑1 (MQ=255) aGTATCAGGGCAACAGATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAAACCTGa > 1:2362221/1‑76 (MQ=255) gTATCAGGGCAACAGATTTCAATGGCAGCGGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGAc < 2:3372630/76‑1 (MQ=255) tATCAGGGCAACAGATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACt < 2:3492998/76‑1 (MQ=255) tATCAGGGCAACAGATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACt < 2:2753982/76‑1 (MQ=255) tATCAGGCCACCAGATTTCAATGGCAGCTGGCACG‑CCA‑CCATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACt < 1:2524134/76‑1 (MQ=255) tcggggCAACAGATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACTaa < 2:3766453/73‑1 (MQ=255) tCAGGGCAACAGATTTCAATGGCGGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACTaa < 1:4047701/76‑1 (MQ=255) tCAGGGCAACAGATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACTaa < 2:5438494/76‑1 (MQ=255) tCAGGGCAACAGATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACTaa < 2:5797027/76‑1 (MQ=255) tCAGGGCAACAGATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACTaa < 1:6474396/76‑1 (MQ=255) gCAACGGATTTAAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACTAACTCCt < 1:575886/76‑1 (MQ=255) gCAACAGATTTCAATGGCAGGTGGCGCG‑CCA‑ACATTAAGTGGT‑CGTTCTTCTCTAAACTAACCATGACTAAATcat > 2:2000506/1‑74 (MQ=255) gCAACAGATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCCTGACTAACTCCt > 2:3540026/1‑76 (MQ=255) cAACAGATTTCAATGGCAGGTGGCACG‑CCC‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCAAGACTAACTCCtt > 2:4193062/1‑76 (MQ=255) cAACAGATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACTCACTCCtt > 1:2030982/1‑76 (MQ=255) cAACAGATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACTAACTCCtt < 1:1992383/76‑1 (MQ=255) cAACAGATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACTAACTCCtt < 1:4475360/76‑1 (MQ=255) aaCAGATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACTAACTCCttt < 1:3556777/76‑1 (MQ=255) aaCAGATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACTAACTCCttt < 1:5032214/76‑1 (MQ=255) aCAGATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACTAACTCCtttt < 1:3093404/76‑1 (MQ=255) cAGATTTCAATGGCAGCTGTCACG‑CCA‑GCATAAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACTAACTCCttttt < 2:1745028/76‑1 (MQ=255) aGATTTCAATGGCAGCTGGCGCG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACTAACTCCtttttt < 1:3639642/76‑1 (MQ=255) aGATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACTAACTCCtttttt < 2:3594422/76‑1 (MQ=255) gATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTa < 2:707438/76‑1 (MQ=255) gATTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTa < 2:3227815/76‑1 (MQ=255) aTTTCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTat < 1:911265/76‑1 (MQ=255) tttCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTatc < 2:5441055/76‑1 (MQ=255) ttCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTatca < 1:5153029/76‑1 (MQ=255) tCAATGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTatcat > 1:5367040/1‑76 (MQ=255) tCAATGGCAGCGGGGAAG‑CCA‑ACATTAAGTGAT‑TGTTCTTCTCTAAACTAACCATCAGTAACTCCTTTTTTattct > 2:1970937/1‑73 (MQ=255) aTGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAAACATGACTAACTCCTTTTTTATCAtctc > 2:4849638/1‑76 (MQ=255) tGGCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATctct > 1:3020934/1‑76 (MQ=255) ggCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTg < 2:6182450/76‑1 (MQ=255) ggCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTg < 2:940132/76‑1 (MQ=255) gCAGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTgg < 1:1880501/76‑1 (MQ=255) aGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTgg < 1:1000859/74‑1 (MQ=255) aGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTgg > 2:1000859/1‑74 (MQ=255) aGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGaa > 1:6225118/1‑76 (MQ=255) aGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGaa < 2:5278838/76‑1 (MQ=255) aGCTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGaa > 1:1313763/1‑76 (MQ=255) cTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAaca > 2:556638/1‑76 (MQ=255) cTGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAaca < 1:3713971/76‑1 (MQ=255) tGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAacac > 1:644438/1‑76 (MQ=255) tGGCACG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAacac > 1:6319048/1‑76 (MQ=255) ggCCCG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAacaca < 2:2471484/76‑1 (MQ=255) | TTGGTTAAATCCTTCAGGCGGGTACCACGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCG‑CCA‑ACATTAAGTGAT‑CGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACA > REL606/3500670‑3500815 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |