| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,164,822 | T→G | F1323V (TTC→GTC) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,164,822 | 0 | T | G | 97.9% | 850.4 / 3.1 | 194 | F1323V (TTC→GTC) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base T (2/2); new base G (96/94); total (98/96) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
Only 100 of 197 total aligned reads displayed.
AACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCA > REL606/4164750‑4164894 | aaCGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCa < 2:3261376/76‑1 (MQ=255)aaCGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCa > 1:1148141/1‑76 (MQ=255)aaCGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCa < 1:712842/76‑1 (MQ=255)aaCGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCa > 2:2364732/1‑76 (MQ=255) aCGGTCTCACCAAGATGTATAAAAACATCTTGGCCGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCaa < 2:602247/76‑1 (MQ=255) ggTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACg < 2:5370995/76‑1 (MQ=255) tCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTa < 1:219689/76‑1 (MQ=255) tCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTa < 1:2413580/76‑1 (MQ=255) cGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTAt > 2:2419373/1‑76 (MQ=255) cGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTAt > 1:2657762/1‑76 (MQ=255) gTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTAtt > 2:6129255/1‑76 (MQ=255) gTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTAtt < 1:5725733/76‑1 (MQ=255) gTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTAtt > 1:2296930/1‑76 (MQ=255) tACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTAttg < 2:6396248/76‑1 (MQ=255) tACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTAttg > 1:6575114/1‑76 (MQ=255) tACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTAttg > 2:5724668/1‑76 (MQ=255) aCCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTAttgt > 1:5778921/1‑76 (MQ=255) aCCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTAttgt > 1:50350/1‑76 (MQ=255) aCCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTAttgt > 2:4814553/1‑76 (MQ=255) ccAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTAttgtt > 1:4461904/1‑76 (MQ=255) cAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTAttgttg < 2:5256077/76‑1 (MQ=255) aaGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGa > 2:4466498/1‑76 (MQ=255) aGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGaa > 2:211795/1‑76 (MQ=255) aGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGaa < 1:1479953/76‑1 (MQ=255) gATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGaaa < 2:6370030/76‑1 (MQ=255) aTGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAag < 2:36777/76‑1 (MQ=255) aTGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAag > 1:2347945/1‑76 (MQ=255) tGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAaga > 1:3244598/1‑76 (MQ=255) tataAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAgaga < 1:5256079/76‑1 (MQ=255) tataAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAgaga > 2:3788542/1‑76 (MQ=255) ataAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCTAGAATCCGTCAACGTATTGTTGAAAGAGAt > 1:6524973/1‑76 (MQ=255) ataAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGAt > 1:6314980/1‑76 (MQ=255) taAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGAtt > 2:2326752/1‑76 (MQ=255) taAAAACATCGGGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGAtt < 1:582524/76‑1 (MQ=255) aaaaaCAGCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGAttc < 2:2177112/76‑1 (MQ=255) aaaaCATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGAttcg > 1:477823/1‑76 (MQ=255) aaaaCATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGAttcg > 1:5225096/1‑76 (MQ=255) aaCATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGAttcgtt < 2:2509921/76‑1 (MQ=255) aCATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGAttcgttc < 2:1100057/76‑1 (MQ=255) aCATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGAttcgttc < 2:566885/76‑1 (MQ=255) cATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGAttcgttcg > 2:1765619/1‑76 (MQ=255) cATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGAttcgttcg < 1:2525168/76‑1 (MQ=255) tCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCt < 2:5106016/76‑1 (MQ=255) tCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCt < 2:4146335/76‑1 (MQ=255) gTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTgg > 1:3301531/1‑76 (MQ=255) tGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTggg > 2:3545609/1‑76 (MQ=255) gACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTa < 1:1021835/76‑1 (MQ=255) acagCAACAATCAGATGCAGCCGGGCATGCCAGAATCAGCAAACATATTGATGAAAAAGATTCATTCGCTGGGTAt < 2:314503/73‑1 (MQ=255) aCGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTAt < 1:3266738/76‑1 (MQ=255) ggCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCa < 1:1904374/76‑1 (MQ=255) ggCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCa < 2:3928393/76‑1 (MQ=255) gCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCaa < 2:350257/76‑1 (MQ=255) gCAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCaa > 2:1907947/1‑76 (MQ=255) cAACCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAAc < 1:2792173/76‑1 (MQ=255) aaCCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACTTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACa > 1:2163546/1‑76 (MQ=255) aaCCATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACa < 2:3381212/76‑1 (MQ=255) ccATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATc < 1:4813672/76‑1 (MQ=255) cATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCg > 1:3390219/1‑76 (MQ=255) cATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCg < 1:3939217/76‑1 (MQ=255) cATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCg < 1:1755805/76‑1 (MQ=255) cATCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCg < 1:3985660/76‑1 (MQ=255) aTCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGa > 1:3562688/1‑76 (MQ=255) aTCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGa < 2:4635381/76‑1 (MQ=255) tCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGaa < 2:3637092/76‑1 (MQ=255) tCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGaa < 2:1004226/76‑1 (MQ=255) tCAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGaa > 1:5600268/1‑76 (MQ=255) cAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGTTTCGCTCGCTGGGTATCAACATCGAAc > 1:4678712/1‑76 (MQ=255) cAGATGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAAc < 2:4245977/76‑1 (MQ=255) tGGAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGa < 2:857838/76‑1 (MQ=255) ggAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGaa < 2:6533183/76‑1 (MQ=255) ggAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGaa < 2:6599627/76‑1 (MQ=255) ggAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGaa > 1:1074388/1‑76 (MQ=255) ggAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGaa < 1:1584455/76‑1 (MQ=255) ggAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGaa < 2:1924711/76‑1 (MQ=255) ggAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGaa > 1:4181189/1‑76 (MQ=255) gAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAg > 1:1642767/1‑76 (MQ=255) gAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAg > 1:1850246/1‑76 (MQ=255) gAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAg < 1:2597655/76‑1 (MQ=255) gAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAg < 1:4576814/76‑1 (MQ=255) gAGCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAg < 2:514331/76‑1 (MQ=255) ggCCGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGa < 1:5455150/75‑1 (MQ=255) ccGGGCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACg < 1:5325187/76‑1 (MQ=255) ggCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGt < 2:4027670/76‑1 (MQ=255) gCATGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTa < 1:345557/76‑1 (MQ=255) cATGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTaa > 2:4581795/1‑76 (MQ=255) aTGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAAt > 2:2934604/1‑76 (MQ=255) aTGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAAt > 2:1527304/1‑76 (MQ=255) aTGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAAt > 2:2314002/1‑76 (MQ=255) tGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAAtt > 1:5157873/1‑76 (MQ=255) tGCCAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAAtt < 1:4310065/76‑1 (MQ=255) ccAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATtct < 2:2226660/76‑1 (MQ=255) ccAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATtct < 2:5937934/76‑1 (MQ=255) ccAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATtct > 2:1255708/1‑76 (MQ=255) cAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATtctc > 2:1880123/1‑76 (MQ=255) cAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATtctc > 2:1619473/1‑76 (MQ=255) cAGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATtctc > 2:3095776/1‑76 (MQ=255) aGAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCg > 2:4944092/1‑76 (MQ=255) gAATCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGc > 2:6287731/1‑76 (MQ=255) tCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCa < 1:2902649/76‑1 (MQ=255) tCCGTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCa > 2:75107/1‑76 (MQ=255) | AACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCA > REL606/4164750‑4164894 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |