| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,004,243 | A→G | intergenic (‑169/+433) | ompF ← / ← asnS | outer membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,004,243 | 0 | A | G | 99.4% | 786.6 / ‑4.2 | 177 | intergenic (‑169/+433) | ompF/asnS | outer membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (68/108); total (69/108) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 | |||||||||||
Only 100 of 198 total aligned reads displayed.
GGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTT > REL606/1004168‑1004315 | ggTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagataaa < 2:4827714/76‑1 (MQ=255)ggTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagataaa < 2:2290775/76‑1 (MQ=255) cTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGAttt > 1:1033787/1‑76 (MQ=255) cTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGAttt < 1:3740908/76‑1 (MQ=255) cTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGAttt > 1:5848734/1‑76 (MQ=255) cTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGAttt > 2:1338083/1‑76 (MQ=255) cTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGAttt < 1:4917390/76‑1 (MQ=255) cTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGAttt > 1:4085171/1‑76 (MQ=255) ttATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTgg > 1:1768016/1‑76 (MQ=255) ttATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTgg > 2:2821501/1‑76 (MQ=255) tATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGt > 1:4533107/1‑76 (MQ=255) tATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGt < 1:5737396/76‑1 (MQ=255) aTGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGtt > 1:1000964/1‑76 (MQ=255) aTGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGtt > 1:6448842/1‑76 (MQ=255) aTGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGtt < 2:402362/76‑1 (MQ=255) aTGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGtt < 2:4599485/76‑1 (MQ=255) tgtgTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGttt > 2:3550655/1‑76 (MQ=255) gtgtCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTc < 2:1110790/76‑1 (MQ=255) gtgtCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTc < 2:5810091/76‑1 (MQ=255) gtgtCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTc < 2:6403467/76‑1 (MQ=255) tCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCaaa > 1:2903602/1‑76 (MQ=255) tCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCaaa < 1:87298/76‑1 (MQ=255) tCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCaaa < 1:1222463/76‑1 (MQ=255) tCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCaaa < 1:3967267/76‑1 (MQ=255) tCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCaaa < 1:3664317/76‑1 (MQ=255) cTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCaaaa > 1:5795684/1‑76 (MQ=255) tGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCaaaaa < 1:4470739/76‑1 (MQ=255) gCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAg < 1:1295557/76‑1 (MQ=255) cAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGa < 2:621009/76‑1 (MQ=255) cAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGa < 2:2963829/76‑1 (MQ=255) aGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGaa > 1:2429374/1‑76 (MQ=255) aGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGaa < 1:4165696/76‑1 (MQ=255) ggCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGaaa < 1:4369240/76‑1 (MQ=255) gCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGaaaa < 1:3454543/76‑1 (MQ=255) cATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGaaaaa < 1:2525658/76‑1 (MQ=255) cATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGaaaaa > 1:4059063/1‑76 (MQ=255) cATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGaaaaa > 2:3377751/1‑76 (MQ=255) cATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAACATAAGGATTTGGTTTCAAAAAGaaaaa > 1:5963059/1‑76 (MQ=255) aTCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAat > 1:3024940/1‑76 (MQ=255) aTCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAat > 1:1716140/1‑76 (MQ=255) cTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCaaa > 1:3424520/1‑65 (MQ=255) cTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAatat > 1:1477382/1‑76 (MQ=255) cTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAatat < 2:6259745/76‑1 (MQ=255) cTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAatat < 1:2395955/76‑1 (MQ=255) tCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTa < 1:6403258/76‑1 (MQ=255) ccATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTaa < 1:1119461/76‑1 (MQ=255) ccATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTaa < 1:3899888/76‑1 (MQ=255) ccATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTaa < 1:2551951/76‑1 (MQ=255) cATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATACGGATTTGGTTTCAAAAAGAAAAATATGTAAc < 1:1900228/76‑1 (MQ=255) aTTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAAcc < 1:5664165/76‑1 (MQ=255) aTTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAAcc > 1:2791270/1‑76 (MQ=255) ttAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCa > 2:2215706/1‑76 (MQ=255) ttAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCa < 1:6549510/76‑1 (MQ=255) aaaaCTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCaaa > 2:3554961/1‑76 (MQ=255) aaaaCTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCaaa > 1:5172934/1‑76 (MQ=255) aaaaCTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCaaa < 1:4856942/76‑1 (MQ=255) aaCTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAg < 1:3877478/76‑1 (MQ=255) aCTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGt > 1:5508236/1‑76 (MQ=255) cTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTa > 2:4473374/1‑76 (MQ=255) cTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTa < 1:998166/76‑1 (MQ=255) cTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTa > 2:3843578/1‑76 (MQ=255) tAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTaa < 2:3732224/76‑1 (MQ=255) aaCGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTaaa > 2:2121625/1‑76 (MQ=255) gcGTGTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGATTTCAAAAAGAAAAATATGTAACCAAAAGTaaaa < 2:1394092/75‑1 (MQ=255) cGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAAt > 1:5294578/1‑76 (MQ=255) gTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAAtt < 1:6526204/76‑1 (MQ=255) gTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAAtt > 2:2564098/1‑76 (MQ=255) cGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAg < 2:5874782/76‑1 (MQ=255) cTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGa < 2:3296015/76‑1 (MQ=255) cTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGa < 1:2192837/76‑1 (MQ=255) tACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGaa < 2:894587/76‑1 (MQ=255) tACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGaa > 1:4642384/1‑76 (MQ=255) tACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGaa > 1:222772/1‑76 (MQ=255) tGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTtg > 1:104328/1‑76 (MQ=255) tGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTtg < 2:342670/76‑1 (MQ=255) tGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTtg > 1:2853809/1‑76 (MQ=255) gAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTtgt > 1:6258232/1‑76 (MQ=255) aaaGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTtgtg > 1:314685/1‑76 (MQ=255) aaGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGa < 2:3094316/76‑1 (MQ=255) aGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGaa < 1:523799/76‑1 (MQ=255) aGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGaa < 2:5991545/76‑1 (MQ=255) aGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGaa < 2:4110704/76‑1 (MQ=255) gTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAac < 2:747390/76‑1 (MQ=255) tGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAaca < 2:5652479/76‑1 (MQ=255) gCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAacac < 1:3222868/76‑1 (MQ=255) gCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAacac < 1:4240621/76‑1 (MQ=255) gCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAacac > 2:5300389/1‑76 (MQ=255) gCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAacac < 1:727106/76‑1 (MQ=255) cTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACAcc < 1:2949957/76‑1 (MQ=255) tACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCg > 2:4563087/1‑76 (MQ=255) aCAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGt > 1:2930876/1‑76 (MQ=255) aaagataagggtTTGGTTTCAAAAGGAAAAATATGTAACCAAAAGTAAAGTTTAAGGTACTTTGTGAGCACCGTct > 2:5184822/1‑75 (MQ=255) aagataaggatTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCat > 2:152274/1‑76 (MQ=255) aagataaggatTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCat < 2:6142405/76‑1 (MQ=255) agataaggatTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCata > 1:6183494/1‑76 (MQ=255) ataaggatTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATAtt > 1:4829973/1‑76 (MQ=255) ataaggatTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATAtt > 2:6495716/1‑76 (MQ=255) taaggatTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATAttt > 1:5548480/1‑76 (MQ=255) taaggatTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATAttt < 1:3774025/76‑1 (MQ=255) taaggatTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATAttt > 2:2741222/1‑76 (MQ=255) | GGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTT > REL606/1004168‑1004315 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |