BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,193,779	G→T	L143I (CTT→ATT)	yeiB ←	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,193,779	0	G	T	100.0%	602.5 / NA	160	L143I (CTT→ATT)	yeiB	conserved inner membrane protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (61/99); total (61/99)

Only 100 of 189 total aligned reads displayed.

GATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193709‑2193851

gATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGGAAATA‑GCCCCCATAACAGC‑AACAAGCCACAccccc                                                                     >  2:4954040/1‑75 (MQ=255)

gATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGGCGTCGGGAAATAG‑CCCCCAAAAACAGA‑AAACACCCAACcccc                                                                      >  1:3649357/1‑76 (MQ=255)

gATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTC‑GGGAATCA‑GCCCCCAAAACAGC‑AAAAAGCCCAAccccc                                                                     >  1:6244796/1‑75 (MQ=255)

gATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGATGTT‑GGGAACAA‑GCCCCCAAAACAGC‑AAAACGCCCAAccccc                                                                     >  1:2782484/1‑75 (MQ=255)

  tGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCAATCCccacc                                                                   <  2:5258161/76‑1 (MQ=255)

   gCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCAATCCccacca                                                                  <  2:4197906/76‑1 (MQ=255)

    cATCCGGCGTCCAGGCGCGGCTGGTCTTGCTGTC‑TGGAATAA‑GACCCAACAAAACA‑CAAAAGCCAAACCccacccc                                                                 >  2:2953723/1‑74 (MQ=255)

    cATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTC‑GGGAAAAA‑GCCCCCATAAAAGC‑AAAAAGCCAAAACCCACCAg                                                                 >  2:2826328/1‑76 (MQ=255)

     aTCCGGCGTCCAGGCGCGGCTGGTGTGGATGGC‑GGAAATTA‑GTCCCCATAACACG‑AAAAAGCCAATTCccaccaca                                                                >  2:5650699/1‑74 (MQ=255)

     aTCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGa                                                                <  1:2518359/76‑1 (MQ=255)

     aTCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGa                                                                <  2:4345684/76‑1 (MQ=255)

     aTCCGGCGTCCAGGCGCGGCTGGTCTGGCTGGC‑GGAAATAC‑GCCCCCACAACACCAAAAACGCAAACCCccaccga                                                                 >  1:3940161/1‑74 (MQ=255)

     aTCCGGCGTCCAGGCGCGGCTGGGCTGGGTGTC‑GGGAAACA‑GCCCCCAAAACAGC‑AAAACGCCAACCCCCCCCCGa                                                                >  1:5843061/1‑76 (MQ=255)

     aTCCGGCGTCCAGGCGCGGCTGGGCTGGGTGGC‑TGAAAACA‑ACCCCCAAAACAGC‑AAAAAACCAAACcccccccaa                                                                >  1:4290023/1‑73 (MQ=255)

     aTCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTT‑GGGAATCA‑GCCCCCAAAACAAC‑AAAACGCCAATACcccccaca                                                                >  2:2692960/1‑74 (MQ=255)

     aTCCGGCGTCCAGGCGCGGCTGGGCCGGCTGTC‑GGGAAACAGGCCCCATAAACACC‑AAAA‑ACCAAACCcccccaca                                                                >  1:430857/1‑74 (MQ=255)

      tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCCCCAGAt                                                               <  2:2329108/76‑1 (MQ=255)

      tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGAt                                                               <  1:6400466/76‑1 (MQ=255)

       ccGGCGTCCAGGCGCGGCTGGTCTGACTGGC‑GGAAATCA‑GCCCCAAAAACAGC‑AAAACGCCAATCCCCACCAGATa                                                              <  1:4981448/76‑1 (MQ=255)

       ccGGCGTCCAGGCGCGGCTGGGCTGGCTGTC‑GGAAATCA‑GCCCCCATAACAGC‑AAAACGCCAATCCCCCCCAGATa                                                              >  1:2910079/1‑76 (MQ=255)

       ccGGCGTCCAGGCGCGGCTGGGCGGGCCGTC‑GGAAATCA‑GCCCCCATAACAAC‑AAAACGCCAATCCCCACCCGaga                                                              >  1:2727023/1‑74 (MQ=255)

        cGGCGTCCGGGCGCGGCTGGTCTGGCTGGC‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATaa                                                             <  2:2757220/76‑1 (MQ=255)

        cGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATaa                                                             <  1:3026174/76‑1 (MQ=255)

        cGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATaa                                                             <  2:2524392/76‑1 (MQ=255)

        cGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATaa                                                             <  2:4622710/76‑1 (MQ=255)

        cGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCA‑GCCCCAATAACAGC‑AAAAAGCCAATCCCCACCAGATaa                                                             <  1:2699612/76‑1 (MQ=255)

         ggCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATaaa                                                            <  1:4143168/76‑1 (MQ=255)

         ggCGTCCAGGCGCGGCTGGGCTGGCTGTC‑GGGAAACA‑ACCCCCATAACAAC‑AAAACGCCAATCCCCACCCCATaaa                                                            >  2:3598446/1‑76 (MQ=255)

          gCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAg                                                           <  1:2677985/76‑1 (MQ=255)

           cGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCTATCCCCACCAGATAAAGc                                                          <  2:4015509/76‑1 (MQ=255)

              ccAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATg                                                       <  1:4108344/76‑1 (MQ=255)

               cAGGCGCGGCTGGGCTGGCTGGC‑GGGAAACA‑GCCCCCAAAAAAGC‑AAAAAACCAAAACCCACCCGATAAAAAATGa                                                      >  2:3403758/1‑76 (MQ=255)

                 ggCGCGGCTGGTCTGGCTGTC‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACg                                                    <  1:4843951/76‑1 (MQ=255)

                 ggCGCGGCTGGTCTGGCTGTC‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACg                                                    <  1:3216820/76‑1 (MQ=255)

                    gcgGCTGGTCTGGCTGTC‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCt                                                 <  1:6552428/76‑1 (MQ=255)

                     cgGCTGGTCTGGCTGTC‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCCATCCCCACCAGATAAAGCATGACGCCTg                                                >  1:6632843/1‑76 (MQ=255)

                      ggCTGGTCTGGCTGTC‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGt                                               <  2:4391526/76‑1 (MQ=255)

                        cTGGTCTGGCTGTC‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt                                             <  2:1879334/76‑1 (MQ=255)

                        cTGGTCTGGCTGTC‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt                                             <  1:5777938/76‑1 (MQ=255)

                        cTGGTCTGGCTGTC‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt                                             <  1:589992/76‑1 (MQ=255)

                        cTGGTCTGGCTGTC‑GGAAATCA‑GCCCCAATAACAGC‑AAAAAGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt                                             >  2:5156936/1‑76 (MQ=255)

                         tGGTCTGGCTGTC‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            <  2:1017947/76‑1 (MQ=255)

                         tGGTCTGGCTGTC‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            <  1:108678/76‑1 (MQ=255)

                         tGGTCTGGCTGTC‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            >  1:1580005/1‑76 (MQ=255)

                         tGGTCTGGCTGTC‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            <  1:6615172/76‑1 (MQ=255)

                         tGGTCTGGCTGTC‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGCAtt                                            >  1:3158085/1‑76 (MQ=255)

                            tCTGGCTGTC‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa                                         >  1:2541993/1‑76 (MQ=255)

                             cTGGCTGTC‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                        <  1:569313/76‑1 (MQ=255)

                             cTGGCTGTC‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                        <  1:3598446/76‑1 (MQ=255)

                             cTGGCTGTC‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                        <  2:2963802/76‑1 (MQ=255)

                              tGGCTGTC‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa                                       <  1:6458774/76‑1 (MQ=255)

                              tGGCTGTC‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa                                       <  2:3542628/76‑1 (MQ=255)

                                  tGTC‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt                                   >  1:1359234/1‑76 (MQ=255)

                                   gTC‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  >  2:1276139/1‑76 (MQ=255)

                                   gTC‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  <  2:2598244/76‑1 (MQ=255)

                                     c‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                                >  2:5219047/1‑76 (MQ=255)

                                     c‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                                >  1:546156/1‑76 (MQ=255)

                                     c‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                                >  2:2851798/1‑76 (MQ=255)

                                        gAAATCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                              <  1:2312244/76‑1 (MQ=255)

                                        gAAATCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                              <  2:2009483/76‑1 (MQ=255)

                                         aaaTCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                             >  1:3290317/1‑76 (MQ=255)

                                         aaaTCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                             >  1:566430/1‑76 (MQ=255)

                                         aaaTCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                             <  2:6487460/76‑1 (MQ=255)

                                          aaTCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAcc                            <  1:2191973/76‑1 (MQ=255)

                                           aTCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg                           <  2:223725/76‑1 (MQ=255)

                                           aTCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg                           <  2:6137392/76‑1 (MQ=255)

                                           aTCA‑ACCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg                           <  2:548481/76‑1 (MQ=255)

                                            tCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGa                          >  2:870428/1‑76 (MQ=255)

                                            tCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGa                          <  1:124529/76‑1 (MQ=255)

                                            tCA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGa                          >  2:146619/1‑76 (MQ=255)

                                             cA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt                         <  1:1699047/76‑1 (MQ=255)

                                             cA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt                         <  1:2026012/76‑1 (MQ=255)

                                             cA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt                         >  1:4530736/1‑76 (MQ=255)

                                             cA‑GCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt                         >  1:6051458/1‑76 (MQ=255)

                                                 ccccAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc                      <  1:1455076/76‑1 (MQ=255)

                                                     aaTAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCAt                  <  1:1653473/76‑1 (MQ=255)

                                                     aaTAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCAt                  >  2:4126951/1‑76 (MQ=255)

                                                      aTAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc                 >  2:5860926/1‑76 (MQ=255)

                                                      aTAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc                 >  2:4378209/1‑76 (MQ=255)

                                                      aTAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGCCGCATc                 <  2:5531283/76‑1 (MQ=255)

                                                       tAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg                >  2:1693017/1‑76 (MQ=255)

                                                       tAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg                <  2:3345407/76‑1 (MQ=255)

                                                       tAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg                >  2:1452481/1‑76 (MQ=255)

                                                       tAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg                <  2:3691971/76‑1 (MQ=255)

                                                         aCAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgcg              >  2:5417611/1‑76 (MQ=255)

                                                          cAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCgcgc             >  2:6395907/1‑76 (MQ=255)

                                                           aGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCa            >  1:46548/1‑76 (MQ=255)

                                                            gC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAc           >  1:4869133/1‑76 (MQ=255)

                                                             c‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAcc          <  2:1174284/76‑1 (MQ=255)

                                                             c‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAcc          >  2:6556149/1‑76 (MQ=255)

                                                               aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         <  2:1123599/76‑1 (MQ=255)

                                                               aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         >  2:2460328/1‑76 (MQ=255)

                                                                aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg        <  2:6051458/76‑1 (MQ=255)

                                                                aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg        <  2:1681932/76‑1 (MQ=255)

                                                                  aCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgcc      >  1:1902230/1‑76 (MQ=255)

                                                                  aCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgcc      <  1:2867795/76‑1 (MQ=255)

                                                                    gCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgc    >  1:2381990/1‑76 (MQ=255)

                                                                     ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg                           >  2:6624831/1‑52 (MQ=255)

                                                                      cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  >  2:3227219/1‑76 (MQ=255)

                                                                      cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  >  1:4260541/1‑76 (MQ=255)

GATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCA‑GCCCCAATAACAGC‑AAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193709‑2193851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`