BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,327,326	G→A	P182S (CCA→TCA)	mreC ←	cell wall structural complex MreBCD transmembrane component MreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,327,326	0	G	A	100.0%	608.5 / NA	154	P182S (CCA→TCA)	mreC	cell wall structural complex MreBCD transmembrane component MreC
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (78/76); total (78/76)

Only 100 of 186 total aligned reads displayed.

TCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAAC  >  REL606/3327253‑3327398

tCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGACa                                                                        >  1:478208/1‑76 (MQ=255)

  aaGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGACAgc                                                                      <  1:3165207/76‑1 (MQ=255)

  aaGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGACAgc                                                                      >  1:3017058/1‑76 (MQ=255)

    gCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGGTATCGTTGCGCAGCACCTGGATTGACAgcgc                                                                    >  1:6473049/1‑76 (MQ=255)

    gCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGACAgcgc                                                                    >  1:4423217/1‑76 (MQ=255)

     cTGCAAATCATCCGTACAACCGTTACCGGCTGCCATTACGCGGATATCGTTGCGCAGCACCTGGATTGACAGcgcg                                                                   >  2:2978591/1‑76 (MQ=255)

     cTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGACAGcgcg                                                                   >  1:1142524/1‑76 (MQ=255)

     cTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGATGCGCAGCAGCTGGATTGACAGcgcg                                                                   >  1:6177111/1‑76 (MQ=255)

      tGCAAATCATCCGTCCAACCGTTCCCGGCTGCAATTACGCGGATATCGTTGCGCAGCCCCTGGATTGACAGCGCGt                                                                  <  1:4141328/76‑1 (MQ=255)

       gCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTg                                                                 >  1:6495279/1‑76 (MQ=255)

        cAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTgg                                                                >  1:694009/1‑76 (MQ=255)

         aaaTCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTggg                                                               >  1:1147876/1‑76 (MQ=255)

         aaaTCATCCGTACAACCGTTACCGGCTGCAATTAAGCGGGTATCGTTGCGCCGCACCCGGATTGACAGCGCGTggg                                                               >  2:3799209/1‑76 (MQ=255)

           atcatcCGTACAACCGTTACCGGCTGCAATTACGCGGATATTGTTGCGCAGCACCTGGATTGACAGCGCGTggggc                                                             >  2:3023074/1‑74 (MQ=255)

           atcatcCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTc                                                             >  1:3267162/1‑76 (MQ=255)

            tcatcCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGGTTGACAGCGCGGGGGGCg                                                            >  1:4490505/1‑76 (MQ=255)

            acatcCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCg                                                            <  2:1944809/75‑1 (MQ=255)

              atcCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCa                                                          >  1:5672358/1‑76 (MQ=255)

               tcCGTACAACCGTTTCCGGCTGCAATTACGCGGATATCCTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCAt                                                         <  2:4977781/76‑1 (MQ=255)

               tcCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCAt                                                         >  2:3695086/1‑76 (MQ=255)

                ccGTACAACCGTTATCGGCTACAATTACGCGGATATCGTTGCGCAGCCCCTGGATTGACAGCGCGTGGGTCGCATc                                                        <  1:4988412/76‑1 (MQ=255)

                ccGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTTGccta                                                        >  2:5601549/1‑73 (MQ=255)

                ccGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATc                                                        >  1:5897197/1‑76 (MQ=255)

                 cGTACAACCGTTACTGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATca                                                       <  2:4596891/76‑1 (MQ=255)

                 cGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATcc                                                       >  1:5086246/1‑75 (MQ=255)

                 cGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATca                                                       <  1:5813740/76‑1 (MQ=255)

                 cGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATca                                                       <  1:4977949/76‑1 (MQ=255)

                  gTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATcac                                                      >  1:1963792/1‑76 (MQ=255)

                   tACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATcaca                                                     >  1:1821292/1‑76 (MQ=255)

                    aCAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACaa                                                    <  2:4333775/76‑1 (MQ=255)

                    aCAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACaa                                                    <  2:5109147/76‑1 (MQ=255)

                     cAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACaaa                                                   <  1:1954516/76‑1 (MQ=255)

                      aaCCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAAt                                                  <  2:2078449/76‑1 (MQ=255)

                      aaCCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAAt                                                  <  2:4679860/76‑1 (MQ=255)

                       aCCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATc                                                 <  2:6121428/76‑1 (MQ=255)

                       aCCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATc                                                 <  1:3385948/76‑1 (MQ=255)

                        ccGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATca                                                <  2:2371669/76‑1 (MQ=255)

                         cGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATcag                                               <  1:3345341/76‑1 (MQ=255)

                          gTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATcagc                                              >  2:6527051/1‑76 (MQ=255)

                          gTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATcagc                                              <  2:3263735/76‑1 (MQ=255)

                           ttACCGGCTGCAATTACGCGGATATCGTTGCGCCGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATcagca                                             >  1:145998/1‑76 (MQ=255)

                            tACCGGCTGCAATTACGCGGATATAGATGCGCAGCACCCGGATTGTCCACGGGATGGTCGCCTCACAAATtagcag                                            >  2:908025/1‑76 (MQ=255)

                             aCCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCagcagc                                           >  1:3093232/1‑76 (MQ=255)

                              ccGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAgcagca                                          <  1:3240571/76‑1 (MQ=255)

                                ggCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCAcg                                        <  1:5367240/76‑1 (MQ=255)

                                ggCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCAcg                                        <  2:1913541/76‑1 (MQ=255)

                                  cTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCAcgcg                                      >  1:3427289/1‑76 (MQ=255)

                                      aaTTACGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTg                                  <  2:1355626/76‑1 (MQ=255)

                                      aaTTACGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTg                                  <  2:4688631/76‑1 (MQ=255)

                                        ttACGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGt                                >  2:115127/1‑76 (MQ=255)

                                        ttACGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGt                                >  2:5979675/1‑76 (MQ=255)

                                         tACGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTc                               <  1:1884795/76‑1 (MQ=255)

                                         tACGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTc                               <  2:6380000/76‑1 (MQ=255)

                                         tACGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTc                               <  1:2978591/76‑1 (MQ=255)

                                         tACGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTc                               >  2:1571928/1‑76 (MQ=255)

                                          aCGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCa                              >  1:2142312/1‑76 (MQ=255)

                                          aCGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCa                              <  1:3702193/76‑1 (MQ=255)

                                          aCGCGGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCa                              >  1:5600933/1‑76 (MQ=255)

                                           cgcgGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAg                             <  1:3749561/76‑1 (MQ=255)

                                           cgcgGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAg                             >  1:2482883/1‑76 (MQ=255)

                                             cgGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGGATCACAAATCAGCAGCACGCGACTGGTCAGtt                           >  2:3520512/1‑76 (MQ=255)

                                             cgGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCCGtt                           >  1:166435/1‑76 (MQ=255)

                                             cgGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGtt                           >  1:2694909/1‑76 (MQ=255)

                                             cgGATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGtt                           >  1:6353680/1‑76 (MQ=255)

                                              ggATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGttt                          <  1:6546574/76‑1 (MQ=255)

                                              ggATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGttt                          >  2:2464158/1‑76 (MQ=255)

                                              ggATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGttt                          <  2:2924186/76‑1 (MQ=255)

                                              ggATATCGTGGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGttt                          <  2:417482/76‑1 (MQ=255)

                                               gATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTa                         <  1:1167275/76‑1 (MQ=255)

                                               gATATCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTa                         <  2:3771152/76‑1 (MQ=255)

                                                atatCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAg                        <  2:2803975/76‑1 (MQ=255)

                                                atatCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAg                        <  1:5185251/76‑1 (MQ=255)

                                                atatCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAg                        <  1:6059069/76‑1 (MQ=255)

                                                atatCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAg                        <  1:2063395/76‑1 (MQ=255)

                                                 tatCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGc                       <  1:5031944/76‑1 (MQ=255)

                                                 tatCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGc                       >  1:6326090/1‑76 (MQ=255)

                                                 tatCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGc                       >  1:6358234/1‑76 (MQ=255)

                                                 tatCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGc                       >  1:3922069/1‑76 (MQ=255)

                                                  atCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCg                      <  2:1716819/76‑1 (MQ=255)

                                                  atCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCg                      >  2:42496/1‑76 (MQ=255)

                                                  atCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCg                      >  1:958478/1‑76 (MQ=255)

                                                  atCGTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCg                      >  2:1240913/1‑76 (MQ=255)

                                                     gTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACg                   <  1:1603104/76‑1 (MQ=255)

                                                     gTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACg                   >  2:384821/1‑76 (MQ=255)

                                                     gTTGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACg                   >  1:5148859/1‑76 (MQ=255)

                                                      ttGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACgg                  >  2:5324202/1‑76 (MQ=255)

                                                       tGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGc                 >  1:2554554/1‑76 (MQ=255)

                                                       tGCGCAGCACCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGc                 >  1:2950302/1‑76 (MQ=255)

                                                          gcagcaCCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGccac              >  1:4638832/1‑76 (MQ=255)

                                                            agcaCCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGccacca            >  1:5229679/1‑76 (MQ=255)

                                                            agcaCCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGAAGGccacca            >  2:3165239/1‑76 (MQ=255)

                                                              caCCTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCaccacc          >  2:5682786/1‑76 (MQ=255)

                                                                ccgggATTGACAGCGAGTGTGGCGTATCACAAAGCAGCAGCACGCGACGAGTCCGTTTAGCGACGGCCCCCACCTg        <  2:597343/73‑1 (MQ=255)

                                                                ccTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTg        <  2:597572/76‑1 (MQ=255)

                                                                ccTGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTg        <  2:1641895/76‑1 (MQ=255)

                                                                  tGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGAc      >  1:1797034/1‑76 (MQ=255)

                                                                  tGGATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGAc      <  1:2373556/76‑1 (MQ=255)

                                                                   ggATTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGAcc     >  2:6613981/1‑76 (MQ=255)

                                                                     aTTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACcaa   <  2:3572627/76‑1 (MQ=255)

                                                                     aTTGACAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACcaa   <  1:5442658/76‑1 (MQ=255)

TCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAAC  >  REL606/3327253‑3327398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`