| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,489,541 | G→A | S171S (AGC→AGT) | glpG ← | predicted intramembrane serine protease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,489,541 | 0 | G | A | 98.4% | 728.6 / ‑1.0 | 190 | S171S (AGC→AGT) | glpG | predicted intramembrane serine protease |
| Reads supporting (aligned to +/- strand): ref base G (2/1); new base A (99/88); total (101/89) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.41e-01 | |||||||||||
Only 100 of 200 total aligned reads displayed.
ACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCAGC > REL606/3489468‑3489613 | aCGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACt < 1:4210108/76‑1 (MQ=255) cGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTa < 2:2850655/76‑1 (MQ=255) cGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTa < 2:4923180/76‑1 (MQ=255) cGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTa < 1:2034823/76‑1 (MQ=255) ggCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTAc > 2:6609327/1‑76 (MQ=255) ggCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTAc > 2:6095494/1‑76 (MQ=255) cccGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCg < 2:3088182/76‑1 (MQ=255) ccGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGa < 2:1523456/76‑1 (MQ=255) cGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGAg > 1:1746884/1‑76 (MQ=255) gCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGAgg < 2:1297161/76‑1 (MQ=255) gAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGt > 2:2905843/1‑76 (MQ=255) aaTTTTTGCTGCACATAGCCGCTTAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGtt < 2:135814/76‑1 (MQ=255) aaTTTTTGCTGCACATAGCCGCTTAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGtt < 2:1954780/76‑1 (MQ=255) aTTTTTGCTGCACATAGCCGCTTAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGttt > 1:5004038/1‑76 (MQ=255) tttttGCTGCACATAGCCGCTTAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGtttt < 2:3922290/76‑1 (MQ=255) tttGCTGCACATAGCCGCTTAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTc > 2:4432538/1‑76 (MQ=255) ttGCTGCACATAGCCGCTTAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTcc < 1:2275342/76‑1 (MQ=255) tgctgcACATAGCCGCTTAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCa < 1:2442733/76‑1 (MQ=255) tgctgcACATAGCCGCTTAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCa > 2:2216487/1‑76 (MQ=255) tgctgcACATAGCCGCTTAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCa < 2:6512510/76‑1 (MQ=255) gctgcACATAGCCGCTTAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCAc > 1:4837193/1‑76 (MQ=255) ctgcACATAGCCGCTTAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCAcc > 2:2995716/1‑76 (MQ=255) ctgcACATAGCCGCTTAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCAcc > 2:2686243/1‑76 (MQ=255) tgtacaTAGACGCTTAACAGGGGGATAATGACAGTAATAAGAATTAGCTTAAAGCTACCGAGGCGTTTTTCAACcg > 2:1603411/4‑76 (MQ=255) tgcACATAGCCGCTTAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACcg > 1:4061532/1‑76 (MQ=255) tgcACATAGCCGCTTAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACcg < 2:3429237/76‑1 (MQ=255) gcACATAGCCGCTTAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACcgc > 1:4768954/1‑76 (MQ=255) gcACATAGCCGCTTAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACcgc > 2:3394755/1‑76 (MQ=255) acaTAGCCGCTTAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCgcgc > 2:1912735/1‑76 (MQ=255) acaTAGCCGCTTAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCgcgc > 2:562341/1‑76 (MQ=255) aTAGCCGCTTAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGcgccg > 1:4949101/1‑76 (MQ=255) aTAGCCGCTTAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGcgccg > 2:6180853/1‑76 (MQ=255) tAGCCGCTTAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACAGcgccgc > 2:4908798/1‑76 (MQ=255) aGCCGCTTAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCgccgcc > 2:1196615/1‑76 (MQ=255) aGCCGCTTAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCgccgcc < 2:5353865/76‑1 (MQ=255) gCCGCTTAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCCCCGCGccgccg < 2:694458/76‑1 (MQ=255) ccGCTTAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCga < 1:1157943/76‑1 (MQ=255) gCTTAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCgaga < 1:1014872/76‑1 (MQ=255) ttAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATa < 1:984841/76‑1 (MQ=255) ttAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATa > 1:6452828/1‑76 (MQ=255) aaCAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATacc > 1:3950168/1‑76 (MQ=255) aaCAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTACAGAGGCGTTTTTCCACCGCGCCGCCGAGATacc > 2:5414810/1‑76 (MQ=255) agcgggCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATacca < 2:2262360/73‑1 (MQ=255) aCAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATacca < 1:2828987/76‑1 (MQ=255) cAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATaccac > 2:244383/1‑76 (MQ=255) aGGGCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATaccacc > 2:4505082/1‑76 (MQ=255) gggCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATaccacca < 1:4588595/76‑1 (MQ=255) gggCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATaccacca < 2:3946883/76‑1 (MQ=255) ggCGCTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATaccaccac < 1:3689841/76‑1 (MQ=255) cgcTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATAccaccacca > 2:2149381/1‑76 (MQ=255) cgcTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATAccaccacca < 1:1727874/76‑1 (MQ=255) cgcTAATGAGAGAAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATAccaccacca > 1:1495487/1‑76 (MQ=255) gcTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCag < 1:4727638/76‑1 (MQ=255) cTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCaga > 2:6419540/1‑76 (MQ=255) cTAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCAACGCGCCGCCGACATACCACCGCCagc > 2:541777/1‑75 (MQ=255) tAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCagag > 1:839333/1‑76 (MQ=255) tAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCagag > 1:1665458/1‑76 (MQ=255) tAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCagag < 1:4050255/76‑1 (MQ=255) tAATGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCagag < 1:5499305/76‑1 (MQ=255) aaTGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCAGAGc < 1:6583637/76‑1 (MQ=255) aaTGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCAGAGc > 1:1446108/1‑76 (MQ=255) aaTGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCAGAGc < 2:1441138/76‑1 (MQ=255) aaTGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCAGAGc > 1:4273625/1‑76 (MQ=255) aaTGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCAGAGc > 2:2732433/1‑76 (MQ=255) aTGAGATTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCAGAGCa < 1:4133447/76‑1 (MQ=255) aTGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGCTCCCACCACCAGAGCa > 1:5177750/1‑76 (MQ=255) tGAGAGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCAGAGCAg > 2:4221993/1‑76 (MQ=255) gagaGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCAGAGCAgg < 2:6558443/76‑1 (MQ=255) gagaGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCAGAGCAgg > 1:182190/1‑76 (MQ=255) gagaGTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCAGAGCAgg < 1:3839854/76‑1 (MQ=255) agagTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCAGAGCAGGt > 2:5893977/1‑76 (MQ=255) gagTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCAGAGCAGGtt < 2:3659879/76‑1 (MQ=255) agTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCCCCACCAGAGCAGGTTa < 2:4768954/76‑1 (MQ=255) gTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCAGAGCAGGTTaa < 1:4397640/76‑1 (MQ=255) gTAATAACAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCAGAGCAGGTTaa < 2:5709567/76‑1 (MQ=255) ataataaCAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGTCGAGATACCACCACCAGAGCAGGTTaa < 1:3440737/75‑1 (MQ=255) taataaCAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCAGAGCAGGTTaaa > 1:1334702/1‑76 (MQ=255) taataaCAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCAGAGCAGGTTaaa > 1:5607064/1‑76 (MQ=255) aataaCAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCAGAGCAGGTTAAag > 2:772831/1‑76 (MQ=255) aataaCAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCAGAGCAGGTTAAag > 2:2322256/1‑76 (MQ=255) aataaCAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCAGAGCAGGTTAAag > 1:2750192/1‑76 (MQ=255) aataaCAATTAGCTTACCACTACAGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCAGAGCAGGTTAAag < 1:5414810/76‑1 (MQ=255) ataaCAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCAGAGCAGGTTAAaga < 1:2226359/76‑1 (MQ=255) ataaCAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCAGAGCAGGTTAAaga > 1:3108083/1‑76 (MQ=255) taaCAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCAGAGCAGGTTAAagag > 1:3329017/1‑76 (MQ=255) aaCAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCAGAGCAGGTTAAAGAgg > 1:3574890/1‑76 (MQ=255) aaCAATTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCAGAGCAGGTTAAAGAgg < 1:6100585/76‑1 (MQ=255) aaCAATGAGCTTACCCCCACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCAGAGCAGGTTAAAGAgg < 1:5883238/76‑1 (MQ=255) aaTTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGata > 1:5361771/1‑76 (MQ=255) aaTTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGata < 1:4505082/76‑1 (MQ=255) aaTTAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGata < 1:294373/76‑1 (MQ=255) ttAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATg < 1:3723760/76‑1 (MQ=255) tAGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGc > 2:4354636/1‑76 (MQ=255) aGCTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCa > 2:5033309/1‑76 (MQ=255) cTTACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATc > 1:192396/1‑76 (MQ=255) ttACCACTAGCGAGGCGTTTTTCCACCTCGCCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCa < 2:1735152/76‑1 (MQ=255) ttACCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCa < 1:6534467/76‑1 (MQ=255) aCCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCAGc > 2:331482/1‑76 (MQ=255) aCCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCAGc > 1:4426498/1‑76 (MQ=255) aCCACTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCAGc > 1:4981149/1‑76 (MQ=255) | ACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTAATGAGAGTAATAACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCGCCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCAGC > REL606/3489468‑3489613 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |