mutation predictions | marginal predictions | summary statistics | genome diff | command line log
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breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| Marginal read alignment evidence (highest frequency 20 of 212 shown, sorted by frequency from high to low) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,515,909 | 0 | A | C | 61.9% | 21.4 / 74.0 | 63 | V260G (GTG→GGG) | ypfI | predicted hydrolase |
| * | REL606 | 2,437,406 | 0 | G | T | 54.3% | 11.1 / 56.9 | 35 | D435E (GAC→GAA) | ypdD | fused predicted PTS enzymes: Hpr component/enzyme I component/enzyme IIA component |
| * | REL606 | 433 | 0 | T | G | 50.0% | 72.7 / 58.4 | 88 | V33G (GTG→GGG) | thrA | bifunctional aspartokinase I/homeserine dehydrogenase I |
| * | REL606 | 1,854,401 | 0 | A | C | 49.4% | 52.1 / 52.8 | 77 | K88N (AAA→AAC) | yeaO | hypothetical protein |
| * | REL606 | 720,464 | 0 | C | G | 44.4% | 8.3 / 15.2 | 18 | A22G (GCC→GGC) | phrB | deoxyribodipyrimidine photolyase, FAD‑binding |
| * | REL606 | 4,180,249 | 0 | T | G | 43.0% | 119.7 / 40.4 | 86 | G24G (GGT→GGG) | yjaH | hypothetical protein |
| * | REL606 | 2,441,705 | 0 | T | G | 41.7% | 77.3 / 11.9 | 60 | T139P (ACC→CCC) | ypdG | predicted enzyme IIC component of PTS |
| * | REL606 | 1,747,903 | 0 | G | T | 41.3% | 455.9 / inf | 375 | A8S (GCA→TCA) | ydiL | hypothetical protein |
| * | REL606 | 1,485,450 | 0 | A | G | 40.9% | 193.6 / 117.4 | 149 | G193G (GGA→GGG) | ydcV | predicted spermidine/putrescine transporter subunit |
| * | REL606 | 2,073,523 | 0 | A | C | 40.4% | 165.9 / 47.6 | 104 | G432G (GGT→GGG) | asmA | predicted assembly protein |
| * | REL606 | 1,118,209 | 0 | G | T | 38.5% | 65.1 / 47.9 | 52 | intergenic (‑444/‑311) | csgD/csgB | DNA‑binding transcriptional activator in two‑component regulatory system/curlin nucleator protein, minor subunit in curli complex |
| * | REL606 | 149,097 | 0 | A | C | 38.2% | 108.9 / 14.7 | 68 | N405T (AAC→ACC) | yadE | predicted polysaccharide deacetylase lipoprotein |
| * | REL606 | 2,213,003 | 0 | A | C | 38.2% | 120.4 / 10.5 | 68 | V184G (GTG→GGG) | fruK | 1‑phosphofructokinase |
| * | REL606 | 2,376,348 | 0 | T | G | 36.9% | 221.1 / 29.0 | 130 | T63P (ACC→CCC) | folC | bifunctional folylpolyglutamate synthase/ dihydrofolate synthase |
| * | REL606 | 2,889,584 | 0 | C | A | 36.2% | 148.1 / 68.5 | 80 | T114N (ACC→AAC) | xdhC | xanthine dehydrogenase, Fe‑S binding subunit |
| * | REL606 | 4,392,556 | 0 | C | T | 35.9% | 134.3 / 72.6 | 118 | L43L (CTA→TTA) | aidB | isovaleryl CoA dehydrogenase |
| * | REL606 | 2,044,357 | 0 | A | C | 35.9% | 328.9 / 85.3 | 170 | V284G (GTG→GGG) | rfbD | dTDP‑4‑dehydrorhamnose reductase subunit, NAD(P)‑binding, of dTDP‑L‑rhamnose synthase |
| * | REL606 | 2,356,014 | 0 | T | G | 35.3% | 257.7 / 37.8 | 136 | T58P (ACC→CCC) | yfbU | hypothetical protein |
| * | REL606 | 4,554,830 | 0 | A | G | 35.3% | 149.2 / 37.0 | 102 | intergenic (+30/+27) | yjiV/mcrC | b4486; conserved protein/5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrC |
| * | REL606 | 2,144,295 | 0 | A | C | 35.0% | 309.9 / 72.6 | 160 | G357G (GGT→GGG) | mrp | antiporter inner membrane protein |
| Marginal new junction evidence (lowest skew 10 of 62 shown) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | REL606 | = 69446 | 362 (1.810) | 124 (0.810) +AACCGGCTTGGGCGTTA |
14/116 | 10.1 | 29.0% | intergenic (‑92/+193) | araD/araA | L‑ribulose‑5‑phosphate 4‑epimerase/L‑arabinose isomerase |
| ? | REL606 | = 4275216 | 427 (2.140) | intergenic (+489/+42) | gltP/yjcO | glutamate/aspartate:proton symporter/hypothetical protein | |||||
| * | ? | REL606 | = 2293939 | 273 (1.370) | 122 (0.790) +AACCGGCTTGGGCGTTA |
13/116 | 10.5 | 31.1% | intergenic (+89/‑153) | nrdA/nrdB | ribonucleotide‑diphosphate reductase alpha subunit/ribonucleotide‑diphosphate reductase beta subunit |
| ? | REL606 | = 4275216 | 427 (2.140) | intergenic (+489/+42) | gltP/yjcO | glutamate/aspartate:proton symporter/hypothetical protein | |||||
| * | ? | REL606 | 4005890 = | 346 (1.730) | 124 (0.790) +ACCGGCTTGGGCGTTA |
13/118 | 10.7 | 29.0% | intergenic (+191/+92) | fre/fadA | NAD(P)H‑flavin reductase/acetyl‑CoA acetyltransferase |
| ? | REL606 | = 4275216 | 427 (2.140) | intergenic (+489/+42) | gltP/yjcO | glutamate/aspartate:proton symporter/hypothetical protein | |||||
| * | ? | REL606 | 2928192 = | 377 (1.890) | 124 (0.790) +ACCGGCTTGGGCGTTA |
13/118 | 10.7 | 28.2% | intergenic (+146/+50) | ygfZ/yqfA | putative global regulator/predicted oxidoreductase, inner membrane subunit |
| ? | REL606 | = 4275216 | 427 (2.140) | intergenic (+489/+42) | gltP/yjcO | glutamate/aspartate:proton symporter/hypothetical protein | |||||
| * | ? | REL606 | = 2294036 | 344 (1.720) | 124 (0.790) +ACCGGCTTGGGCGTTA |
13/118 | 10.7 | 29.1% | intergenic (+186/‑56) | nrdA/nrdB | ribonucleotide‑diphosphate reductase alpha subunit/ribonucleotide‑diphosphate reductase beta subunit |
| ? | REL606 | = 4275216 | 427 (2.140) | intergenic (+489/+42) | gltP/yjcO | glutamate/aspartate:proton symporter/hypothetical protein | |||||
| * | ? | REL606 | = 69531 | 317 (1.590) | 118 (0.770) +AACCGGCTTGGGCGTTA |
12/116 | 11.0 | 29.2% | intergenic (‑177/+108) | araD/araA | L‑ribulose‑5‑phosphate 4‑epimerase/L‑arabinose isomerase |
| ? | REL606 | = 4275216 | 427 (2.140) | intergenic (+489/+42) | gltP/yjcO | glutamate/aspartate:proton symporter/hypothetical protein | |||||
| * | ? | REL606 | 5563 = | 323 (1.620) | 118 (0.770) +AACCGGCTTGGGCGTTA |
12/116 | 11.0 | 29.0% | intergenic (+35/+118) | yaaX/yaaA | hypothetical protein/hypothetical protein |
| ? | REL606 | = 4275216 | 427 (2.140) | intergenic (+489/+42) | gltP/yjcO | glutamate/aspartate:proton symporter/hypothetical protein | |||||
| * | ? | REL606 | = 2374606 | 304 (1.520) | 120 (0.750) +CCGGCTTGGGCGTTA |
12/120 | 11.2 | 29.2% | intergenic (‑251/+8) | cvpA/dedD | membrane protein required for colicin V production/hypothetical protein |
| ? | REL606 | = 4275216 | 427 (2.140) | intergenic (+489/+42) | gltP/yjcO | glutamate/aspartate:proton symporter/hypothetical protein | |||||
| * | ? | REL606 | 114280 = | 340 (1.700) | 118 (0.750) +ACCGGCTTGGGCGTTA |
11/118 | 11.5 | 28.2% | intergenic (+43/+173) | mutT/yacG | nucleoside triphosphate pyrophosphohydrolase, marked preference for dGTP/zinc‑binding protein |
| ? | REL606 | = 4275216 | 427 (2.140) | intergenic (+489/+42) | gltP/yjcO | glutamate/aspartate:proton symporter/hypothetical protein | |||||
| * | ? | REL606 | 917509 = | 227 (1.140) | 80 (0.540) +GCAACCGGCTTGGGCGTTA |
7/112 | 13.2 | 24.8% | intergenic (‑227/+64) | artJ/artM | arginine transporter subunit/arginine transporter subunit |
| ? | REL606 | = 4275216 | 427 (2.140) | intergenic (+489/+42) | gltP/yjcO | glutamate/aspartate:proton symporter/hypothetical protein | |||||