| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,131,466 | (A)9→8 | intergenic (‑125/+175) | gatY ← / ← fbaB | D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,131,458 | 0 | A | . | 98.0% | 490.7 / 1.7 | 100 | intergenic (‑117/+183) | gatY/fbaB | D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base . (80/18); total (81/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 158 total aligned reads displayed.
AAATGATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACATATG > REL606/2131383‑2131533 | aaatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCa > 1:5546948/1‑76 (MQ=255)aaatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCa > 2:6398698/1‑76 (MQ=255)aaatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCa < 2:1797400/76‑1 (MQ=255) aatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaa < 2:7445370/76‑1 (MQ=255) aatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaa < 2:3734598/76‑1 (MQ=255) aatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaa < 1:7295228/76‑1 (MQ=255) aatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaa < 2:2417869/76‑1 (MQ=255) aatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaa < 1:7746895/76‑1 (MQ=255) atgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaa > 1:6583259/1‑76 (MQ=255) tgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaa > 1:2960012/1‑76 (MQ=255) gaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaa > 1:5493378/1‑76 (MQ=255) aTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaa < 1:1244467/76‑1 (MQ=255) aTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaa < 1:1345228/76‑1 (MQ=255) aTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaa > 1:1954669/1‑76 (MQ=255) ttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaa > 2:7983818/1‑76 (MQ=255) ttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaa > 1:6230495/1‑76 (MQ=255) ttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaa < 2:2374742/76‑1 (MQ=255) ttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaa < 2:3141724/76‑1 (MQ=255) ttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaa > 2:5828629/1‑76 (MQ=255) gtGTTTTTAAATGGCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGTAATGAAAGCCaaaaaaaa < 2:2374741/75‑1 (MQ=255) tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaac < 2:2039798/76‑2 (MQ=255) tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaac > 1:4558293/1‑75 (MQ=255) gTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaacg < 2:1546543/76‑3 (MQ=255) tttttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaacgg < 1:5856132/76‑4 (MQ=255) tttttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaacgg > 2:6889636/1‑73 (MQ=255) tttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGcc > 1:6857563/1‑76 (MQ=255) ttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCt > 1:2807369/1‑76 (MQ=255) ttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCt > 2:1668394/1‑76 (MQ=255) ttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCt > 1:172008/1‑76 (MQ=255) ttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCt > 1:4908882/1‑76 (MQ=255) tAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTc > 2:1449018/1‑76 (MQ=255) aaaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTcc < 2:4994443/76‑1 (MQ=255) aaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGcccccc > 1:7072963/1‑76 (MQ=255) aaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTccc > 2:3025582/1‑76 (MQ=255) aaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTccc > 2:6945092/1‑76 (MQ=255) aaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTccc > 2:5772370/1‑76 (MQ=255) tGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCgg > 2:7433925/1‑76 (MQ=255) gTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGa > 1:4792415/1‑76 (MQ=255) tCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAg > 1:745289/1‑76 (MQ=255) cGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAgg > 1:2506813/1‑76 (MQ=255) cGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAgg > 1:950930/1‑76 (MQ=255) cGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAgg < 2:3759118/76‑1 (MQ=255) agagaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAgg < 1:4642857/75‑1 (MQ=255) gagaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAggg > 1:6975521/1‑76 (MQ=255) gagaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAggg < 2:6075012/76‑1 (MQ=255) gagaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAggg > 2:3542779/1‑76 (MQ=255) gagaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAggg > 1:6272373/1‑76 (MQ=255) aTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAg < 1:5102032/76‑1 (MQ=255) tAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGc > 1:1931989/1‑76 (MQ=255) aCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGc < 2:4733610/74‑1 (MQ=255) aCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGc > 1:4733610/1‑74 (MQ=255) aTCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGcaa > 1:6966912/1‑76 (MQ=255) cacaAAAACAAAAATAAAAAAATAAATAGAGAAATGAAAGGC‑AAAAAAACCGGCCCCCCGGAGGGAAGCCGcaaca < 1:1126697/76‑1 (MQ=255) caAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACaaa > 1:5620350/1‑76 (MQ=255) aaaaaaCAAAAATATAAAAATAAAAAGAGGAAAAAAAGG‑AAAAAAAACCGCCCCCCGGGGGGAAAGCCGCAACaaa < 2:2164016/75‑1 (MQ=255) aaaaaCGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAg > 1:7651717/1‑76 (MQ=255) aaaCGAAAATATGAAAATTAATAGAGGAAAGAAAGG‑AAAAAAAAACGGCCCCCCGGGGGAAAGCCGCAACAAAGTg < 2:5764187/76‑1 (MQ=255) aaaCAAAAATATAAAAATAAAAAGAGAAAT‑AAAAGAAAAAAAAACCGGCCCCCGGGAGGAAAGCCGCAACAAAGTg < 2:62628/76‑1 (MQ=255) aCGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTgcg > 2:2339482/1‑76 (MQ=255) cGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTgcgc > 2:4109728/1‑76 (MQ=255) aaaaaTGTGAAAATAAATAGGGGAAT‑AAAAGAAAAAAAAAACGGCCCCCGGGAGGAAAGCCGCAACAAAGTGcgcg < 2:2684856/73‑1 (MQ=255) aaaaTATAAAAATTAATAGAGGAATAAAAGG‑AAAAAAAAACGCCCCCCGGGAGGAAAGCCGCAACAAAGTGCGCGt < 2:7383493/74‑1 (MQ=255) aCAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGt > 2:7773512/1‑76 (MQ=255) aCAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGt > 1:2384172/1‑76 (MQ=255) aCAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGt > 2:5503203/1‑76 (MQ=255) cAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTg > 2:5255480/1‑76 (MQ=255) cAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTg > 2:2463913/1‑76 (MQ=255) aaTATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTgg > 1:6979660/1‑76 (MQ=255) tatGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGtt > 2:3866495/1‑76 (MQ=255) tatGAAAATTAATAGAGGAATAAAAGG‑AAAAAAACACGGCCCCCCGGGGGGAAGCCGCAACAAAGTGCGCGTGGtt < 2:6169714/76‑1 (MQ=255) tGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAAACGCAACAAAGAGCGCGTGGgttt > 2:6360565/1‑76 (MQ=255) gAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTa > 1:1213529/1‑76 (MQ=255) gAAAATTAATAGAGAAAGAAAAGGC‑AAAAAAACCGGCCCCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTa < 1:3444704/76‑1 (MQ=255) aaaaTAAATAGAGAAATGAAAGG‑AAAAAAAACCGCCCCCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTAt < 1:2071374/76‑1 (MQ=255) aaaTTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATg > 1:6740969/1‑76 (MQ=255) aaaTTAAGAGAGGAATAAAAGG‑AAAAAAAACCGGCCCCCGGGAGGAAAGCCGCAACAAAGTGCGCGTGGTTTTATg < 2:6575499/76‑1 (MQ=255) aaTTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGc > 2:3398938/1‑76 (MQ=255) ataaaTAGGGGAATGAAAGGC‑AAAAAAACCGGCCCCCGGGGGGAAAGCCGCAACAAAGTGCGCGTGGTTTTATGCa < 2:3903795/73‑1 (MQ=255) aTTAAAAGAGAAATGAAA‑GAAAAAAAAACCGGCCCCCGGGAGGAAAGCCGCAACAAAGTGCGCGTGGTTTTATGCa < 2:2870989/76‑1 (MQ=255) ttAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAg > 2:7156159/1‑76 (MQ=255) ttAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAg > 2:3196320/1‑76 (MQ=255) ttAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAg > 2:7698170/1‑76 (MQ=255) aTAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGAt > 1:4654424/1‑76 (MQ=255) tAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATg > 2:2849325/1‑76 (MQ=255) agagGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgc > 1:180323/1‑76 (MQ=255) agagGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgc > 1:438266/1‑76 (MQ=255) agagGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgc > 2:3576006/1‑76 (MQ=255) agaaaTGAAAGG‑AAAAAAAACCGGCCCCCGGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcgg < 1:6396547/73‑1 (MQ=255) agGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcgg > 1:1669277/1‑76 (MQ=255) agGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcgg > 2:7214168/1‑76 (MQ=255) gAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcggcg > 1:5317823/1‑76 (MQ=255) aaaTAAAAGG‑AAAAAAAACCGCCCCCCGGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcggcg < 1:2819835/75‑1 (MQ=25) aaTGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGt > 2:6628453/1‑76 (MQ=255) aTGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGAAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTa > 1:3964169/1‑76 (MQ=39) tGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTaa > 1:136441/1‑76 (MQ=255) aaaGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAAc > 2:3058660/1‑76 (MQ=255) aaaGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAAc > 1:6106256/1‑76 (MQ=255) aaGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAACCa > 1:1964247/1‑76 (MQ=38) agaaaaaaaaagCGGCCCCCGGGGGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACat < 1:1127931/73‑1 (MQ=37) aaaaaaaacCGGCCTCCGGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACATAtg < 1:1566617/76‑1 (MQ=255) | AAATGATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACATATG > REL606/2131383‑2131533 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |