| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,875,632 | (T)7→8 | intergenic (‑66/+287) | glmU ← / ← atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,875,625 | 1 | . | T | 98.7% | 608.2 / 0.3 | 151 | intergenic (‑59/+294) | glmU/atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base T (62/87); total (63/88) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01 | |||||||||||
Only 100 of 185 total aligned reads displayed.
ATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑TT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGC > REL606/3875550‑3875694 | aTAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACa > 2:1621344/1‑76 (MQ=255) tAGCATTATTCAACATGCGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑t < 1:6795477/76‑1 (MQ=255) tAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑t < 2:3451701/76‑1 (MQ=255) tAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑t < 1:2426197/76‑1 (MQ=255) aGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑tt < 2:2184384/76‑1 (MQ=255) aGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑tt < 2:2703593/76‑1 (MQ=255) aGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑tt > 2:2471786/1‑76 (MQ=255) aGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑tt > 1:2460127/1‑76 (MQ=255) attattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑tt‑ttt > 2:4825441/1‑76 (MQ=255) ttattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑tt‑tttt > 2:6725364/1‑76 (MQ=255) tattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑tt‑ttttt > 1:930608/1‑76 (MQ=255) attCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑tt‑tttttt > 1:2792598/1‑75 (MQ=255) attCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑tt‑tttttt < 1:5212663/76‑2 (MQ=255) tCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑tt‑ttttttca > 2:1327334/1‑73 (MQ=255) aCATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCg > 2:5510577/1‑76 (MQ=255) aTACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTg > 1:7138301/1‑76 (MQ=255) aTACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTg > 1:6629444/1‑76 (MQ=255) aTACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTg < 1:3317465/76‑1 (MQ=255) aTACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTg > 2:3933651/1‑76 (MQ=255) gcgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAAt < 2:6184022/76‑1 (MQ=255) gTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑TT‑TTTTTCATCGTGAAATGGa < 2:262320/76‑1 (MQ=255) tCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGa < 1:394888/76‑1 (MQ=255) ccTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGAc > 2:1537205/1‑76 (MQ=255) cTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACa > 1:1526883/1‑76 (MQ=255) cTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACa > 2:4739650/1‑76 (MQ=255) cTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACa > 1:4562254/1‑76 (MQ=255) cTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACa < 1:5782173/76‑1 (MQ=255) tGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACag < 2:7469712/76‑1 (MQ=255) tGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACag > 2:4889756/1‑76 (MQ=255) tGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACag > 2:5117944/1‑76 (MQ=255) tGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACag < 2:7070801/76‑1 (MQ=255) gACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCGTCGTGAAATGGACaga > 1:5602137/1‑76 (MQ=255) gACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACaga > 1:5815391/1‑76 (MQ=255) aCTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACagag < 2:5653439/76‑1 (MQ=255) aCTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACagag > 1:1379362/1‑76 (MQ=255) tGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGa > 1:5368342/1‑76 (MQ=255) gTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGAt < 2:6327628/76‑1 (MQ=255) gTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGAt < 2:1165062/76‑1 (MQ=255) aaTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATaa < 1:2890629/76‑1 (MQ=255) aaTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATaa < 1:2693452/76‑1 (MQ=255) aaTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATaa < 1:3096527/76‑1 (MQ=255) ttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATgg < 1:1198568/62‑1 (MQ=255) ttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAAtt > 2:1523233/1‑76 (MQ=255) ttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAAtt < 2:1043059/76‑1 (MQ=255) ttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAAtt < 2:1086664/76‑1 (MQ=255) tGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTg > 1:2924609/1‑76 (MQ=255) tGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTg > 2:3606056/1‑76 (MQ=255) gagaACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGt > 2:888823/1‑76 (MQ=255) gaACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTc < 1:6057988/76‑1 (MQ=255) gaACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTc < 2:1639010/76‑1 (MQ=255) gaACGAATTTAAACCGCATCACCTTGTAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTc < 2:3974730/76‑1 (MQ=255) cGAATTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAAt < 2:636663/76‑1 (MQ=255) gAATTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATa > 1:1090651/1‑76 (MQ=255) gAATTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATa > 2:5768871/1‑76 (MQ=255) aaTTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATaa < 2:5965423/76‑1 (MQ=255) aaTTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATaa > 2:4124027/1‑76 (MQ=255) aaTTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATaa < 2:2941829/76‑1 (MQ=255) aaTTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATaa < 2:6037412/76‑1 (MQ=255) aTTTAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAAc > 2:360031/1‑76 (MQ=255) tttAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACg < 2:2070197/76‑1 (MQ=255) ttAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACgg < 2:6633763/76‑1 (MQ=255) ttAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACgg < 1:3191312/76‑1 (MQ=255) tAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCATTAACGGa > 2:2007888/1‑76 (MQ=255) tAAACCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGa > 1:4177046/1‑76 (MQ=255) aaaCCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAt < 1:7074900/76‑1 (MQ=255) aaCCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAtt < 2:2742616/76‑1 (MQ=255) aaCCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAtt < 2:6233983/76‑1 (MQ=255) aaCCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAtt < 2:7738584/76‑1 (MQ=255) aaCCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAtt < 1:1545760/76‑1 (MQ=255) aCCGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTa < 2:613674/76‑1 (MQ=255) ccGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTAt < 2:1127044/76‑1 (MQ=255) ccGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTAt < 1:1393501/76‑1 (MQ=255) ccGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTAt < 2:4564750/76‑1 (MQ=255) ccGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTAt < 1:4805231/76‑1 (MQ=255) ccGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTAt < 2:4492212/76‑1 (MQ=255) ccGCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTAt < 2:2961158/76‑1 (MQ=255) gCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATcc < 1:978593/76‑1 (MQ=255) gCTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATcc < 1:7213616/76‑1 (MQ=255) cTTCACCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATccc > 1:502239/1‑76 (MQ=255) aCCTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCt < 2:5855148/76‑1 (MQ=255) ccTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTg > 2:3369247/1‑76 (MQ=255) ccTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTg < 1:6690623/76‑1 (MQ=255) ccTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTg < 1:4120204/76‑1 (MQ=255) ccTTGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTg < 2:6514736/76‑1 (MQ=255) tGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACg > 1:1985669/1‑76 (MQ=255) tGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACg > 1:1610641/1‑76 (MQ=255) tGAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACg < 2:3908351/76‑1 (MQ=255) gAAAAAACTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGa > 1:7332432/1‑76 (MQ=255) aaaaaaCTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaa > 2:7575369/1‑76 (MQ=255) aaaaaaCTACA‑TTGTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaa < 2:1247049/76‑1 (MQ=255) aaaaaCTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaaa < 2:7429842/76‑1 (MQ=255) aaaaaCTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaaa < 1:7725251/76‑1 (MQ=255) aaaaaCTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaaa < 2:7904282/76‑1 (MQ=255) aagcTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAgc > 1:7934518/4‑76 (MQ=255) aaaCTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAgc > 2:7680743/1‑76 (MQ=255) aaCTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAgcg > 1:2257314/1‑76 (MQ=255) aaCTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAgcg > 1:1433011/1‑76 (MQ=255) aaCTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAgcg > 1:4510187/1‑76 (MQ=255) aCTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAgcgc > 1:1140872/1‑76 (MQ=255) aCTACATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAgcgc < 1:6816789/76‑1 (MQ=255) | ATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑TT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGC > REL606/3875550‑3875694 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |