| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,854,401 | 0 | A | C | 40.5% | 130.7 / 36.6 | 89 | K88N (AAA→AAC) | yeaO | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (21/32); new base C (0/36); total (21/68) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.17e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 135 total aligned reads displayed.
TTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGCGGACAT‑CGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACG > REL606/1854327‑1854475 | tttGGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGCGGACAT‑CGCCAAAAAAc < 2:6094607/76‑1 (MQ=255)tttCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGCGGACAT‑CGCCAAAAAAc < 1:692835/76‑1 (MQ=255)tttCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGCGGACAT‑CGCCAAAAAAc < 2:505572/76‑1 (MQ=255)tttCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGCGGACAT‑CGCCAAAAAAc < 2:4605923/76‑1 (MQ=255)tttCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGCGGACAT‑CGCCAAAAAAc < 2:2951683/76‑1 (MQ=255) tCGCGAGCAATATCTTGCAGAACTGGCGCATCACGAGCAAGAAGGAAAGCGGCT‑GGCGGACAT‑CGCCAAAAAACAg < 1:1675134/76‑1 (MQ=255) tCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGGGGGGATCCGCCaaaaaaaa > 2:220058/1‑74 (MQ=255) tCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGCGGACAT‑CGCCAAAAAACAg < 1:6442205/76‑1 (MQ=255) tCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGCGGACAT‑CGCCAAAAAACAg < 1:2620418/76‑1 (MQ=255) cgcgAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGGGGGCAT‑CGCCCCAAAACAga > 1:844604/1‑75 (MQ=255) gCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGTT‑GGCGGGCAT‑CGCCAAAAAACAGCCGccg > 2:6819674/1‑74 (MQ=255) cAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGCGGACAT‑CGCCAAAAAACAGCCGCTGa < 1:5411573/76‑1 (MQ=255) cAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGCGGACAT‑CGCCAAAAAACAGCCGCTGa < 1:3162782/76‑1 (MQ=255) ttCTGGCAGAACGGGCGCAACACGAGCAAAAAGGAAAGCGGCT‑GGCGGACAC‑CGCAAAAAACCAGCCGCTGACCCt < 2:3586320/75‑1 (MQ=255) atCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGCGGACAT‑CGCCAAAAAACAGCCGCTGACCCt < 1:1925314/76‑1 (MQ=255) atCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGCGGACAT‑CGCCAAAAAACAGCCGCTGACCCt < 1:3358035/76‑1 (MQ=255) atCTTGCAGAACGGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGCGGACAT‑CGCCAAAAAACAGCCGCTGACCCt < 1:5623005/76‑1 (MQ=255) tCTTGCAGAACTGGCGCAACACGAGCAAGAGGGAAAGCGGCT‑GGCGGACAT‑CGCCAAAAACCAGCCGCTGACCCTg < 2:6333525/76‑1 (MQ=255) tCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGGGGGGAC‑CGCCACAAAAAAACCCCCGAccccc > 1:5797290/1‑74 (MQ=255) gCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGCGGACAT‑CGCCAAAAAACAGCCGCTGACCCTGctct < 1:5874619/76‑1 (MQ=255) cAGACCTGGCGCAACAGGAGCAAGAAGGAAAGCGGCT‑GGCGGACAT‑CGCCAAAAAACAGCCGCTGACCCTGCTCTa < 1:741257/76‑1 (MQ=255) cAGAACGGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGCGGACAT‑CGCAAAAAACCAGCCGCTGACCCTGCTCTa < 2:6957210/76‑1 (MQ=255) aGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGGGGGGCA‑CGCCCAAAAAAAACCGCCGGCCCCGCTCCAc > 2:3039596/1‑76 (MQ=255) aaCGGGCACAACACGAGCAAGAAGGAAAGCGGCT‑GGCGGCCAT‑CGCCAAAAAACAGCCGCTGACCCTGCTCTACgc < 2:3915535/76‑1 (MQ=255) acgggCGCAACACGAGCAAGAAGGAAAGCGGCG‑GGCGGACAT‑CGCCAAAAAACAGCCGCTGACCCTGCTCTACgca < 1:1880322/73‑1 (MQ=255) aCTGGCGAAACAGGAGAAAGAAGGAAGGCGGCT‑GGCGGACAT‑CTCAAAAAACCAGCCGCTGACCCTGCTCTACgca < 2:6384178/76‑1 (MQ=255) cTGGCGAACCACGAGCAAGAAGGAAAGCGGCT‑GGCGGACAT‑CGCCAAAAACCAGCCGCTGACCCTGCTCTACgcag < 2:7369626/76‑1 (MQ=255) tGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGCGGACAT‑CGCCAAAAAACAGCCGCTGACCCTGCTCTACgcagc < 1:5319195/76‑1 (MQ=255) tGGCGCAACACGAGAAAGAAGAAAAGCGGCG‑GGCGGACAT‑CGCCAAAAACCAGCCGCTGACCCTGCTCTACgcagc < 2:1179373/76‑1 (MQ=255) gcgcAACAGGAGCAAGAAGGAAAGCGGCT‑GGTGCCCAT‑CCCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCaa < 2:7943214/76‑1 (MQ=255) gcgcAACACGAGAAAGAAGAAAAGCGGCT‑GGCGGACAT‑CGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCaa < 2:7848344/76‑1 (MQ=255) cgcAACACGAGCAAGAAGGAAAGCGGCT‑GGGGGGGAT‑CCCCCCAAAAAAAACCCTGCCCCCGCTCCTCCCAGCaaa > 1:1491086/1‑76 (MQ=255) gcAACACGAGCAAGAAGGAAAGCGGCT‑GGCGGACAT‑CGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCaaaa < 1:3883697/76‑1 (MQ=255) aaCAGGAGAAAGAAGAAAGGCGGCT‑GGCGGACAT‑CGCCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCaaaaaa < 2:6156407/76‑1 (MQ=255) aaCACGAGCAAGAAGGAAAGCGGCT‑GGCGGACAT‑CGCAAAAAAACAGCCGCTGACCCTGCTCTACGCAGCaaaaaa < 1:6627430/76‑1 (MQ=255) aaCACGAGAAAGAAGGAAAGGGGCG‑GGCGGACAT‑CGCAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCaaaaaa < 2:2905614/76‑1 (MQ=255) caggagaAAGAAGGAAAGCGGCT‑GGCGGACAT‑CGCCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAaca < 2:1688556/73‑1 (MQ=255) gCAAGAAGAAAAGCGGGT‑GCCGAACAT‑CGCCAAAAATCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACg < 2:1541647/76‑1 (MQ=255) ccAAGGAGAAAGGCGGCC‑GGCGGACAC‑CACCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACg < 2:1501556/75‑1 (MQ=255) aaaagaagGAAAGCGTCT‑GGCGCACAT‑CGCCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACg < 2:4352411/74‑1 (MQ=255) cAAGAAGGAAAGCGGCTGGGGGGAGTT‑CCGCAAAAAAAAG‑CGCGGACGCCGCCCTCCTCAGCAAAAAAaaacaccc > 2:5867690/1‑74 (MQ=255) cAAGAAGGAAAGCGGCT‑GGCGGACAT‑CGCCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGc < 1:5595692/76‑1 (MQ=255) aaagaagGAAAGCGGCG‑GGCGGAAAT‑CGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGc < 1:3596851/75‑1 (MQ=255) aaagaagAAAAGCGGCT‑GGCGGACAT‑CGCCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGc < 2:5408655/75‑1 (MQ=255) aagaggAAAAGCGGCT‑GGCGGAAAG‑CGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCa < 2:1349416/76‑1 (MQ=255) aagaagGAAAGCGGCT‑GGCGGACAT‑CGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCa < 1:1416612/76‑1 (MQ=255) aagaagAAAGGCGGCG‑GCCGGAAAC‑CGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCa < 1:3291893/76‑1 (MQ=255) agaagaAAGGGGGCT‑GGCGGACAT‑CGCCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAg < 1:679604/76‑1 (MQ=255) agaagaAAAGCGGCT‑GGCGGACAT‑CGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAg < 1:1834783/76‑1 (MQ=255) gaagaaAGGCGGCT‑GGCGGACAT‑CGCCAAAAACCAGCCCCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGa < 2:1162440/76‑1 (MQ=38) aagGAAAGCGGCT‑GGGGGGGAA‑CGCCCAAAAAAAACCCCCGACCCCGCTCTCCCCCGCAAAAAAAAACACACACaa > 2:2098413/1‑76 (MQ=255) aagGAAAGCGGCT‑GGCGGACAT‑CGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGaa < 1:6807084/76‑1 (MQ=255) aagAAAGGCGGCT‑GGCGAACAT‑CGCAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGaa < 2:2203181/76‑1 (MQ=255) ggAGAGCGGCG‑GGCGGACAT‑CGCCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAAcc < 2:7393980/76‑1 (MQ=255) gaaaaGCGGCT‑GGCGGACAT‑CCCCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAAcc < 1:1812884/74‑1 (MQ=255) gAAAGCGGCG‑GCCGAACAG‑CGCAAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCa < 1:2827085/76‑1 (MQ=255) aaaGCGGCT‑GGGGGACAT‑CCCAAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCAt < 1:7114839/76‑1 (MQ=255) aggCGGCT‑GGCGGACAT‑CGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATg < 1:3699799/74‑1 (MQ=255) aGCGGCT‑GGCGGACAT‑CGCCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATgc < 2:4542856/76‑1 (MQ=255) gCGGCT‑GGCGGACAT‑CGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATgcg < 1:4800764/76‑1 (MQ=255) gCT‑GGCGGACAT‑CGCCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTg < 2:4742446/76‑1 (MQ=255) gCT‑GGCGGACAT‑CGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTg > 1:3632604/1‑76 (MQ=255) cT‑GGCGGACAT‑CGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTgg > 1:150914/1‑76 (MQ=255) t‑GGCGGACAT‑CGCCAAAAAACAGCCGCTGCCCCTGCTCTACGCAGCAAAAAACACCACGCAGCACCATGCGCTGGt > 1:5170553/1‑76 (MQ=255) t‑GGCGGACAT‑CGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGt > 2:3430109/1‑76 (MQ=255) t‑GGCGGACAT‑CGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGt > 1:1660316/1‑76 (MQ=255) ggCGGACAT‑CGCCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTg < 2:5648917/76‑1 (MQ=255) ggCGGACAT‑CGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAACCACCACGCAGAACCATGCGCTGGTg > 2:5177937/1‑76 (MQ=255) ggCGGACAT‑CGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTg > 2:5016115/1‑76 (MQ=255) cGGCCAT‑CACAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCt < 2:2234087/76‑1 (MQ=255) ggACAT‑CGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTg > 2:2291414/1‑76 (MQ=255) gACAT‑CGCAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTgg < 2:171897/76‑1 (MQ=255) gACAT‑CGCAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTgg < 2:3434085/76‑1 (MQ=255) gACAT‑CGAAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTgg < 2:1491086/76‑1 (MQ=255) gACAG‑CGCAAAAACCCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTgg < 2:365184/76‑1 (MQ=255) cAT‑CGCCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGcc < 2:4780217/76‑1 (MQ=255) cAT‑CGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGcc > 2:4598077/1‑76 (MQ=255) ct‑CGCAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCg < 2:2239998/75‑1 (MQ=255) aT‑CGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCg > 2:7070389/1‑76 (MQ=255) t‑CGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGa > 1:5125138/1‑76 (MQ=255) ccGCAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGa < 2:6460971/75‑1 (MQ=255) cGCAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGAc < 1:2163469/76‑1 (MQ=255) ccaaaaaacCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACt < 1:743698/73‑1 (MQ=255) ccAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTg > 1:975495/1‑76 (MQ=255) ccAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTg > 1:7717223/1‑76 (MQ=255) ccAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTg > 1:1161804/1‑76 (MQ=255) ccAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTg > 1:3774445/1‑76 (MQ=255) caaaaaacCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTg < 1:4540839/74‑1 (MQ=255) caaaaaacCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTg < 1:133285/74‑1 (MQ=255) aaaacCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCt < 1:2850362/76‑1 (MQ=255) aaaacCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCt < 1:7829573/76‑1 (MQ=255) aaaacCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCt < 1:4704498/76‑1 (MQ=255) aaaacCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCt < 1:1173476/76‑1 (MQ=255) aaaaaCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCt > 1:2395140/1‑76 (MQ=255) aaacCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTa < 1:6116791/76‑1 (MQ=255) aaacCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTa < 1:3713937/76‑1 (MQ=255) aaaaCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTa > 1:6764751/1‑76 (MQ=255) aaaaCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTa > 2:6124541/1‑76 (MQ=255) aaCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACg > 2:7487065/1‑76 (MQ=255) aaCAGCCGCTAACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTAcc > 1:7291119/1‑75 (MQ=255) | TTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCT‑GGCGGACAT‑CGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACG > REL606/1854327‑1854475 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |