| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,437,406 | 0 | G | T | 38.6% | 65.5 / 27.0 | 44 | D435E (GAC→GAA) | ypdD | fused predicted PTS enzymes: Hpr component/enzyme I component/enzyme IIA component |
| Reads supporting (aligned to +/- strand): ref base G (14/13); new base T (17/0); total (31/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.73e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 103 total aligned reads displayed.
TCTTCCTGGGGAATATTCAGATAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTG‑TCGCCCGCCGCCAGTAGCACCTGCTGGTAGGC‑TTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCAT > REL606/2437332‑2437480 | tcttctTGGGGACTATTGAGATGAGTAATTCTTTTACCCCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTG‑t < 2:4426982/76‑1 (MQ=255) ctTCCTGGGGAATATTCAGATAAGGAATGTTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTG‑Tc < 2:6016288/76‑1 (MQ=255) ttCGTGGGGAATATTCAGATAAGGAATGCTTTTACCCCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTG‑Tcg < 2:7826219/76‑1 (MQ=255) ttCCTGGGGAATATTCAGATAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTT‑Tcg > 2:3306506/1‑76 (MQ=255) ttCCGGGGGAATATTCAGATAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTG‑Tcg < 2:493018/76‑1 (MQ=255) tCCTGGGGAATATTCAGATAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTT‑Tcgc > 1:1942296/1‑76 (MQ=255) tCCTGGGGAATATTCAGAGAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTG‑Tcgc < 1:7060600/76‑1 (MQ=255) ccTGGGGAATATTCAGATAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTG‑Tcgcc < 1:7978332/76‑1 (MQ=255) cTGGGGAATATTCAGATAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTG‑Tcgccc < 1:4519747/76‑1 (MQ=255) tGGGGAATATTCAGATAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTT‑Tctcccc > 1:5404576/1‑75 (MQ=255) tGGGGAATATTCAGATAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTT‑Tcgcccc > 2:7166275/1‑75 (MQ=255) tGGGGAATATTCAGATAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTG‑Tcgcccg < 1:1411110/76‑1 (MQ=255) ggggAATATTCAGATAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTT‑Ttgccccc > 1:2775711/1‑74 (MQ=255) ggggAATATTCAGATAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTT‑Tcgcccgc > 2:6627380/1‑76 (MQ=255) gggaAATATTCAGATAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTG‑Tcgcccgc < 1:822389/76‑1 (MQ=255) gggAATATTCAGATAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTT‑Ttgcccgcc > 1:6250713/1‑76 (MQ=255) ggAATATTCAGATAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTT‑Tttcccgccc > 1:2200515/1‑75 (MQ=255) ggAATATTCAGATAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTT‑Ttgccccccc > 1:6199453/1‑75 (MQ=255) aaTATTCAGATAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTT‑TTTCCCcccgcc > 2:1359534/1‑76 (MQ=255) aaTATTCAGATAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTT‑TTGCCCgccgcc > 1:5690698/1‑76 (MQ=255) aaTATTCAGATAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTT‑TCGCcccccccc > 2:578205/1‑76 (MQ=255) aaTATTCAGATAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTG‑TTGCCCcccgcc > 2:6715508/1‑76 (MQ=255) aaTATTCAGATAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTC‑TCGCcccccccc > 2:7483657/1‑76 (MQ=255) aaTATTCAGATAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTA‑TTGCACgccgcc > 1:2340580/1‑76 (MQ=255) aaTATTCAAATAAGGATTCTTTTTCTCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTG‑TCGCCCgccgcc < 2:5656768/76‑1 (MQ=255) atatTCAGATAAGGAATGTTTTTACCCCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTG‑TCGCCCGCCGCCa < 1:3287145/76‑1 (MQ=255) atatTCAGATAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTT‑TTGCccccccccc > 1:541764/1‑75 (MQ=255) atatTCAGATAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTT‑TTGCccccccccc > 2:736640/1‑75 (MQ=255) atatTCAGATAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTG‑TTGCCCgcccccc > 2:1908111/1‑75 (MQ=255) atatTCAGATAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTG‑TTGCCCGCCGCCa > 1:5320803/1‑76 (MQ=255) tatTCAGATAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGCAGATCATCTGCCTT‑TCGCCCgcccccgc > 2:4770501/1‑74 (MQ=255) tatTCAGATAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTT‑TCGCCCgccgcccg > 2:6694960/1‑74 (MQ=255) tatTCAGATAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTG‑TTGCCCGCCCCCAg > 2:5282550/1‑76 (MQ=255) tatTCAGATAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTG‑TCGCcccccccccg > 1:165990/1‑74 (MQ=255) tatTCAGATAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTG‑TCGCCCgccgcccg > 2:2233476/1‑74 (MQ=255) tatTCAGATAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTG‑TCGCCCgccgcccg > 2:4770502/1‑74 (MQ=255) tagtCAGATAAGAAATGTTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTG‑TCGCCCGCCGCCAg < 1:3051501/73‑1 (MQ=255) atTCAGATAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTT‑TCcccccccccccag > 1:3108670/1‑73 (MQ=255) atTCAGATAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTT‑TCGGCCgccccccgc > 1:1939312/1‑73 (MQ=255) tCAGATAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTG‑TCGCCCCCCGCcagcag > 1:5664782/1‑76 (MQ=255) aTAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGCAGATAATCGGCTTT‑TAGCCCGCCCCCCGGTGCAcc > 2:4862867/1‑76 (MQ=255) aTAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTT‑TTGCCCGCCGCCCGTAGCAcc > 1:329154/1‑76 (MQ=255) aTAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTT‑TCGCCCCCCGCCCCGAGCAcc > 2:2060962/1‑76 (MQ=255) aTAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTGTTTGCCCGCC‑CCCGCAGCAcc > 1:289163/1‑76 (MQ=255) aTAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGAGGAAGATAATCGGCTTT‑TCCCCCGCCTCCCGTTGGAcc > 1:4172029/1‑76 (MQ=255) tAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTT‑TCGCCCGCCGCCAGTAGCAccc > 1:3057467/1‑75 (MQ=255) tAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTT‑TCGCCCGCCCCCCCTAGCAccc > 2:4647644/1‑75 (MQ=255) aaGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTT‑TTGCCCGCCCCCCGCAGCAcccc > 2:4035648/1‑74 (MQ=255) aaGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTT‑TTGCCCGCCCCCCCGAGCAcccg > 1:2576427/1‑74 (MQ=255) aaGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTT‑TCGCCCGCCCCCCGCAGCACctg > 1:2044489/1‑76 (MQ=255) aGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTT‑TCGCCCCCCGCCAGCAGCACccgc > 1:2023970/1‑76 (MQ=255) ggAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTT‑TTGCCCGCCGCCAACAGCACccgct > 2:4174405/1‑76 (MQ=255) gAATGTTTTTCCCCCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTG‑TCGCCCGCCGCCAGTAGCACctgctg < 1:3851553/76‑1 (MQ=255) aTGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTT‑TTGCCCCCCGCCCGACGCACccgctcgg > 1:4374682/1‑73 (MQ=255) aTGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTT‑TCGCCCCCCCCCCGGAGCACCCGCTggg > 1:4015503/1‑75 (MQ=255) aTGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTT‑TCGCCCCCCCCCCCGAGCCCCCGCTggg > 1:1445882/1‑75 (MQ=255) tGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTG‑TCGCCCCCCGCCCGGCGCACCCGCTggga > 1:5335214/1‑74 (MQ=255) gCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTT‑TTGCCCCCCCCCCGCAGGACCCGCTggggg > 1:5053482/1‑73 (MQ=255) cTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTT‑TCGCCCCCCGCCAGTAGAGCCTACTGGAAgg > 2:5974415/1‑76 (MQ=255) ttttATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTT‑TCGGCCCCCCCCCGTCGCACCCGCTCGTGGGc > 2:1294984/1‑76 (MQ=255) tttATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTT‑TCGCCCCCCGCCAGTAGCACCTGCTGGGAGGC‑t > 1:3817345/1‑76 (MQ=255) tttATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTG‑TCGCCCCCCCCCACGAGGACCCGCTGGGAgggt > 1:1422496/1‑74 (MQ=255) aTCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTT‑TCGCCCGCCCCCCGGAGCACCTGCTGGGAGGG‑TTCa > 1:261635/1‑76 (MQ=255) aTCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTT‑TCGCCCGCCCCCAGTAGCACCTGCTGGTAGGG‑TTCa > 1:1939770/1‑76 (MQ=255) tCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTT‑TTGCCCCCCGCCCCGAGCACCCGCTCGGAGGG‑TGCaa > 1:1683373/1‑76 (MQ=255) cccccgccgATGCCCATCGGGGGGAAGATAATCGGTTTG‑CCCCCCGCCGCCAGTAGCACCTGCTGGTAGGC‑TTCaa < 1:4582334/73‑1 (MQ=255) ccgccgTTGCCCACCGGGCGAAAGATAAGCGGCTTG‑TCCCCCGCCGCCAGTAGCACCTGCTGGTAGGC‑TTCAAAtt < 1:62407/76‑1 (MQ=255) ccgccgATGTCCATCGTGCGGAAGATAATCGGCTTT‑TTGCCCCCCCCCCGTAGCACCTGCTGGTAGGC‑TTCcaaat > 2:3945451/1‑74 (MQ=255) ccgccgATGTCCATCGTGCGGAAGATAATCGGCTTT‑TCGCCCGCCCCCCGTAGCACCCGCTGGGAGGG‑TTTCAAtt > 1:6304456/1‑76 (MQ=255) ccgccgATGTCCATCGTGCGGAAGATAATCGGCTTT‑TCGCCCCCCGCCCGTAGCACCCGCTGGGAGGCTTTCAAAt > 2:1764322/1‑76 (MQ=255) gccgATGTCCATCGTGCGGAAGATAATCGGCTTT‑TCGCCCGCCCCCAGTAGCACCTGCTGGGAGGG‑CTCCAAtttt > 2:3562658/1‑74 (MQ=255) cgATGTCCATCGTGCGGAAGATAATCGGCTTT‑TTGCCCGCCGCCAGTAGCACCTGCTGGGAGGC‑TTTAAAATtctc > 2:6808945/1‑76 (MQ=255) cgATGTCCATCGTGCGGAAGATAATCGGCTTT‑TCGCCCGCCCCCAGGAGGACCCGCTGGGAGGG‑TTCAAAATtctc > 1:6000970/1‑76 (MQ=255) aTGTCCATCGTGCGGAAGATAATCGGCTTT‑TCTGCCGCCCCCAGAAGGACCTGCGGGGAGGC‑TTCAAATTTCTccc > 2:4295554/1‑75 (MQ=255) aTGTCCATCGTGCGGAAGATAATCGGCTTG‑TCGCCCGCCGCCAGTAGCACCTGCTGGTAGGC‑TTCAAATTGCTCCt < 1:3885817/76‑1 (MQ=255) aTGTCCATCGTGCGGAAGATAATCGGCTTG‑TCGCCCGCCCCCCGTAGCACCTGCTGGGAGGG‑TTCAAAATGCTCCt > 2:2244501/1‑76 (MQ=255) tGTCCATCGTGCGGAAGATAATCGGCTTT‑TCGCCCGCCGCCCGTAGCACCTGCTGGGAGGG‑TTCAAAATTCTCCtc > 2:1412719/1‑75 (MQ=255) tGTCCATCGTGCGGAAGATAATCGGCTTT‑TCGCCCGCCCCCAGTAGCACCTGCTGGGAGGG‑TTCTAAATACTCCtc > 2:6115867/1‑75 (MQ=255) tGTCCATCGTGCGGAAGATAATCGGCTTT‑TCGCCCCCCGCCAGTAGCACCTGCTGGGAGGCTTTAAAATTGCCCCt > 1:2040930/1‑76 (MQ=255) gTCCATCGTGCGGAAGATAATCGGCTTT‑TCGCCCGCCCCCAGTAGCACCCCCTGGTGGGG‑CTCAAATTTCTCCCGc > 1:239632/1‑76 (MQ=255) ccttCGGGCGAAAGATATTCGGTTGG‑CCCCCCGCCGCCAGTAGCACCTGCTGGTAGGC‑TTCAAATTGCTCCTGCTc < 2:5559235/73‑1 (MQ=255) ccATCGTGCGGAAGATAATCGGCTTT‑TCGCCCGCCCCCAGTAGCACCCGCCGGTAGGG‑TTCCAATTTCTCCTgtct > 1:5291404/1‑73 (MQ=255) cATCGTGCGGAAGATAATCGGCTTT‑TTGCCCGCCGGCAGTTGCACCTGCTGGGAGGC‑TTCTAAATGCTGctcctcc > 2:1759859/1‑75 (MQ=255) aTCGTGCGGAAGATAATCGGCTTG‑TCGCCCCCCGCCCCTAGCACCCGCTGGTGGGG‑TTCCAATTTCTCCTGCTCgc > 1:2517705/1‑75 (MQ=255) ggagCGGAAGTTAATGGTCTGC‑GCCCCCGCCGCCAGTAGCACCTGCTGGTAGGC‑TTCAAATTGCTCCTGCTCGTcc < 2:5708903/73‑1 (MQ=255) gggCGAAAAATATTCGTCTTG‑CCGCCCGCCGCCAGTAGCACCTGCTGGTAGGC‑TTCAAATTGCTCCTGCTCGTCCg < 1:2286743/74‑1 (MQ=255) ggCGAAAGATAAGCGGCTTG‑TCCCCCGCCGCCAGTAGCACCTGCTGGTAGGC‑TTCAAATTGCTCCTGCTCGTCCgg < 2:1606945/75‑1 (MQ=255) ggagAAGATAATGGGGTTG‑GCCCCCGCCGCCAGTAGCACCTGCTGGTAGGC‑TTCAAATTGCTCCTGCTCGTCCGgc < 2:1430370/73‑1 (MQ=255) cGGAAGATAATCGGCTTT‑TCGCCCCCCCCCAGTCACACCTTCGGGGTCGC‑GTCAAAATGGTCCCGCTCGTCCcgcg > 2:3281665/1‑76 (MQ=255) aaGATAATCGGCTTT‑TTGCCCCCCCCCCGGAGGACCCGCTGGGAGGG‑TTCCAAATTCTCCCGCTCCTCccgcccgc > 2:6464072/1‑76 (MQ=255) cTTG‑TCGCCCGCCGCCAGTAGCACCTGCTGGTAGGC‑TTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATcc > 1:5190283/1‑76 (MQ=255) cTTG‑TCGCCCGCCGCCAGTAGCACCTGCTGGTAGGC‑TTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATcc > 1:936227/1‑76 (MQ=255) cTTG‑TCGCCCGCCGCCAGTAGCACCTGCTGGTAGGC‑TTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATcc > 1:3672771/1‑76 (MQ=255) ttG‑TCGCCCGCCGCCAGTAGCACCTGCTGGTAGGC‑TTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCa > 2:1152967/1‑76 (MQ=255) ttG‑TCGCCCGCCGCCAGTAGCACCTGCTGGTAGGC‑TTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCa > 2:5340635/1‑76 (MQ=255) ttG‑CCGCCCGCCGCCAGTAGCACCTGCTGGTAGGC‑TTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCa < 1:5633848/76‑1 (MQ=255) tG‑TCGCCCGCCGCCAGTAGCACCTGCTGGTAGGC‑TTCAAATTGCTCCTGCTCGTCCGTCGCGCTGTCGCGATCCat > 1:1751558/1‑76 (MQ=255) tG‑TCGCCCGCCGCCAGTAGCACCTGCTGGTAGGC‑TTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATccct > 1:250638/1‑74 (MQ=255) tG‑TCGCCCGCCGCCAGTAGCACCTGCTGGTAGGC‑TTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCat > 2:3757030/1‑76 (MQ=255) tG‑TCGCCCGCCGCCAGTAGCACCTGCTGGTAGGC‑TTCAAATTGCTCCTGCTCGTCCGGCGCCCTGTCGCGATccct > 2:6561830/1‑74 (MQ=255) | TCTTCCTGGGGAATATTCAGATAAGGAATGCTTTTATCGCCGCCGATGTCCATCGTGCGGAAGATAATCGGCTTG‑TCGCCCGCCGCCAGTAGCACCTGCTGGTAGGC‑TTCAAATTGCTCCTGCTCGTCCGGCGCGCTGTCGCGATCCAT > REL606/2437332‑2437480 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |