| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,217,434 | 0 | G | C | 35.6% | 48.1 / 33.0 | 59 | A76P (GCC→CCC) | agaS | tagatose‑6‑phosphate ketose/aldose isomerase |
| Reads supporting (aligned to +/- strand): ref base G (36/2); new base C (13/8); total (49/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.46e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 114 total aligned reads displayed.
AGAGAATCTGCGGATCATCCTGACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCGCCAGCCATA‑CCGGTAAAAACTTCAGCGCCGTACCGACCACCGATCTGGTCACCAATCCGATGGACTACCTGAACC > REL606/3217361‑3217509 | agagAATCTGCGGATCATCCTGACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCgcc < 2:1388285/76‑1 (MQ=255) agaATCTGCGGATCATCCTGACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTcccccc > 2:2346907/1‑74 (MQ=255) agaATCTGCGGATCATCCTGACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCgccag < 2:1409314/76‑1 (MQ=255) gtttCTGCGGATCATCCTGACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCgccagc > 2:3920887/4‑76 (MQ=255) gtttCTGCGGATCATCCTGACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCccccgc > 2:2041638/4‑76 (MQ=255) gaATCTGCGGATCATCCTGACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCgccagc < 1:5960541/76‑1 (MQ=255) aaTCTGCGGATCATCCTGACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCgccagcc < 2:7536346/76‑1 (MQ=255) aaTCTGCGGATCATCCTGACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCgccagcc < 2:6628867/76‑1 (MQ=255) aTCTGCGGATCATCCTGACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCgccagcca < 2:7812596/76‑1 (MQ=255) aTCTGCGGATCATCCTGACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCgccagcca < 1:3897480/76‑1 (MQ=255) aTCTGCGGATCATCCTGACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCccccgccc > 1:4861839/1‑75 (MQ=255) aTCTGCGGATCATCCTGACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCccccgccc > 1:5275562/1‑75 (MQ=255) tCTGCGGTTCATCCTGCCCGGAGCCGGAACGTCGGCTTTTATGGGTGACATCATCGCGCCGGGGCTCGCCAGCCAt < 2:7279873/76‑1 (MQ=255) cTGCGGATCATCCTGACCGGAGCCGGAACGTCGGCATTTATCGGTGACCTCATCGCGCCGGGGCTCgccagccctt > 2:6885614/1‑73 (MQ=255) tGCGGATCATCCTGACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCGCCACGCATA‑c > 2:2018827/1‑76 (MQ=255) gcgcATCCTCCGGCCGGGGGCGGGAACGTCGGCATTTTTCGGTGCCATCGGCGCGCCGGGCCCCCCCAGCCATA‑cc < 2:111679/76‑1 (MQ=255) gCGGATCATCCTGACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCCCCCGCCATA‑cc > 2:111255/1‑76 (MQ=255) gCGGATCATCCTGACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCCCCAGCCCTA‑cc > 1:5729765/1‑76 (MQ=255) cGGTTCATCCGGCCGGGCGCCGAGCCGTCGGCTTTAATCGTGGACAACGCCGCGCCGTGGCTCCCCAGCCATA‑CCg < 2:1157282/76‑1 (MQ=255) cGGATCATCCTGACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCGCCCGCCACA‑CCg > 2:3258196/1‑76 (MQ=255) cGGATCATCCTGACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCCCCCGCCCTA‑CCg > 2:2451660/1‑76 (MQ=255) gATCATCCTGACCGGAGCCGGAACTTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCCCCCCCCATA‑CCgcg > 2:2195434/1‑74 (MQ=255) atcatcCTGACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCGCCCACCCTA‑CCCGTa > 1:5685662/1‑76 (MQ=255) atcatcCTGACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCGCCAGCCACA‑CCggga > 2:3085320/1‑74 (MQ=255) tcatcCTGACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCGCCACCCCTA‑CCgggca > 1:7420051/1‑73 (MQ=255) catcCTGACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCGCCCGCCCTA‑CCCGTaaa > 2:671661/1‑76 (MQ=255) catcCTGACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCGCCCGCCCCA‑CCGGTcaa > 2:4493353/1‑76 (MQ=255) catcCTGACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCGCCAGCCAAA‑CCGGGaaa > 2:4878404/1‑76 (MQ=255) catcCTGACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCCCCCGCCCTC‑CCGGCaaa > 2:7854311/1‑76 (MQ=255) catcCTGACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCCCCCGCCCAA‑CCGGGaaa > 2:7607668/1‑76 (MQ=255) catcCTGACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCCCCCGCCACA‑CCCGGaaa > 2:4393484/1‑76 (MQ=255) catcCTGACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCCCCACCCACA‑CCGGTaaa > 2:5647066/1‑76 (MQ=255) atcCTGACCGGAGCCGGAACGTCGGCCGTTATCGGTGACATCCTCGCGCCGTGGCGCCCCCGCCCAA‑CCCGGaaaa > 1:4128881/1‑76 (MQ=255) atcCTGACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCGCCCGCCCTA‑CCCGCaaaa > 1:1553061/1‑76 (MQ=255) atcCTGACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCCCCCGCCCTA‑CCGGTaaaa > 1:3797948/1‑76 (MQ=255) atcCTGACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCCCCCGCCCTA‑CCCGTaaaa > 1:3263775/1‑76 (MQ=255) atcCTGACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCCCCCGCCCTA‑CCCGGaaaa > 1:3620577/1‑76 (MQ=255) ccTGCCCGGACCCGAAGCGTGGTCTTTAATGGGTGACATCTCCGCCCGGTCCCCCCCCAGCCATA‑CCGGTAAAAAc < 2:4265784/76‑1 (MQ=255) ccTGACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGGGCCGGGGCTCGCCCCCCACC‑CCGGTCAAAAc > 1:2909170/1‑76 (MQ=255) ccTGACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCCCCCGCCACA‑ACGGTaaaaaa > 2:4715554/1‑75 (MQ=255) ccTGACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCCCCAGCCACA‑CCCGGaaaaaa > 1:7657410/1‑75 (MQ=255) cTGACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCGCCCGCCATA‑CCGGGaaaaaat > 1:4895232/1‑74 (MQ=255) tGACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCCCCAGCCATA‑CCGGTAAAAAAtt > 1:7361805/1‑76 (MQ=255) tGACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCCCCAGCCATA‑CCCGGCAAAAcct > 2:4096276/1‑74 (MQ=255) gcccGGCGCCGGACCGCCGGCTTTTAGCGGTCACTTCACCCCCCCGGGGCCCCCCAGCCATA‑CCGGTAAAAACTTc < 1:5915759/74‑1 (MQ=255) gcccGGAGCGGGACCGGCGGCTTTTATGGGTGACACCACCGCGCCGGGGCCCCCCAGCCATA‑CCGGTAAAAACTTc < 1:249702/74‑1 (MQ=255) gACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCGCCCGCCATA‑CCGGCAAAAACATc > 2:5393931/1‑76 (MQ=255) cccGGGGCGGGACCGTGGGCTTTTATGGGTGACACCCCCGCCCGGTGCCCCCCCAGCCATA‑CCGGTAAAAACTTCa < 2:4952547/75‑1 (MQ=255) cgggAGCGGGAACGTCGTCATTTATGGGTAACATCCCCGCCCGGGGCCCCCCCAGCCATA‑CCGGTAAAAACTTCAg < 2:2873294/74‑1 (MQ=255) ggAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCGCCAGCCCTA‑CCGGTAAAAAATTCAgcg > 2:3759218/1‑76 (MQ=255) ggAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCGCCAGCCCTA‑CCCGTAAAAACTTCatcc > 2:4726718/1‑73 (MQ=255) gAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCGCCAGCCCTA‑CCGGTAAAAACTTCAgcgc > 2:5519489/1‑76 (MQ=255) gAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCGCCAGCCATA‑CCGGGAAAAAATTTCgcgc > 1:7234684/1‑76 (MQ=255) ccGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCCCCCGCCCTACCCGGAAAAAATTTCA‑CGCCgc > 1:4873021/1‑75 (MQ=255) cGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCGCCATCCATA‑CCGGCAAAAACTTTCGCGCCGTa > 2:3704589/1‑76 (MQ=255) cGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCCCCCGCCAAA‑CCCGTAAAAACTTCCGCGCCgga > 2:4862475/1‑74 (MQ=255) accGTGGGCTTTTACCGGTCACATCACCGCGCCGGGGCCCCCCAGCCATA‑CCGGTAAAAACTTCAGCGCCGTACCg < 1:4557880/74‑1 (MQ=255) aaCGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCCCCCGCCATA‑CCCGGAAAAAATTCAGCGCCCCACCg > 2:4495949/1‑76 (MQ=255) tCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCCCCAGCCATA‑CCGGTAAAAACTTCAGCGCCGTACCGacca > 1:7868743/1‑76 (MQ=255) cGGCATTTATCGGTGACATCATCGCGCCGTGGCTCCCCAGCCATA‑CCGGTAAAAACTTCAGCGACGTACCGaccac > 2:7600183/1‑76 (MQ=255) cGGCATTTATCGGTGACATCATCGCGCCGTGGCTCCCCAGCCATA‑CCGGTAAAAACCTCAGCGCCGTACCGaccac > 2:4538544/1‑76 (MQ=255) gCATTTATCGGTGACATCATCGCGCCGTGGCTCCCCAGCCCCA‑CCCGGAAAAAACTCACCGCCCTACCCaccaccc > 1:403785/1‑75 (MQ=255) cATTTATCGGTGACATCATCGCGCCGTGGCTCGCCAGCCATA‑CCGGTAAAAACTTCAGCGCCGTACCGaccaccag > 1:6486927/1‑74 (MQ=255) aTTTATCGGTGACATCATCGCGCCGTGGCTCGCCAGCCCTA‑CCGGCAAAAACTTCAGCGCCGTACCGcccacccac > 1:204381/1‑73 (MQ=255) aTTTATCGGTGACATCATCGCGCCGTGGCTCGCCAGCCATA‑CCGGGAAAAACTTCAGCGCCGTACCGACCACCgct > 1:1747602/1‑74 (MQ=255) tttATCGGTGACATCATCGCGCCGTGGCTCGCCCGCCATA‑CCGGTAAAAACTTCAGCGCCGTACCGCCCCCCGATc > 1:6245238/1‑76 (MQ=255) tttATCGGTGACATCATCGCGCCGTGGCTCGCCAGCCATA‑CCGGTAAAAAAATCAGCGCCGTACCGACCACCGATc > 1:7873038/1‑76 (MQ=255) tttATCGGTGACATCATCGCGCCGTGGCTCGCCAGCCATA‑ACGGGAAAAACTTCAGCGCCGTAACGACCACCCATc > 2:7234635/1‑76 (MQ=255) tttATCGGTGACATCATCGCGCCGTGGCTCCCCAGCCCTA‑CCGGTAAAAACTTCAGCGCCGGACCGACCACCGATc > 2:7131780/1‑76 (MQ=255) aTCGGTGACATCATCGCGCCGTGGCTCGCCAGCCCAA‑CCCGCAAAAACATCCACGCCGTACACACCACCGAACtcg > 2:285504/1‑74 (MQ=255) aTCGGTGACATCATCGCGCCGTGGCTCGCCAGCCATA‑CCGGTAAAAACTTCAGCGCCGTACCGACCCCCCATCTgg > 2:1487552/1‑76 (MQ=255) aTCGGTGACATCATCGCGCCGTGGCTCGCCAGCCATA‑CCGGTAAAAACTTCAGCGCCGTACCGACCCACGCTCTgg > 2:4051735/1‑76 (MQ=255) aTCGGTGACATCATCGCGCCGTGGCTCGCCAGCCATA‑CCGGTAAAAACTTCAGCGCCGGACCCACCACCCATCTgg > 2:4681937/1‑76 (MQ=255) aTCGGTGACATCATCGCGCCGTGGCTCCCCAGCCATA‑CCGGGCAAAAATTCAGCGCCGTACCGGCCACCGCTCTgg > 2:1738415/1‑76 (MQ=255) tCGGTGACATCATCGCGCCGTGGCTCCCCAGCCCTA‑CCGGGAAAAACTTCAGCGCCGTACCGACCACCGATCTGGt > 1:3527198/1‑76 (MQ=255) tCGGTGACATCATCGCGCCGTGGCTCCCCAGCCATA‑CCCGGAAAAACTTCAACGCCGTAACGACCACCCAACTgct > 1:2451180/1‑74 (MQ=255) ggTGCCATCCCCGCCCCGTGCCCCCCCAGCCATA‑CCGGTAAAAACTTCAGCGCCGTACCGACCACCGATCTGGTCa < 1:2732685/76‑1 (MQ=25) ggTGACATCATCGCGCCGTGGCTCGCCAGCCATA‑CCGGGAAAAACTTCAGCGCCGTACCGACCACCGATCTGGTcc > 1:7476533/1‑75 (MQ=255) gTGACATCATCGCGCCGTGGCTCGCCAGCCCTA‑CCGGTAAAAACTTCAGCGCCGTACCGACCACCGATCTGGTCAc > 2:6728911/1‑76 (MQ=255) gTGACATCATCGCGCCGTGGCTCGCCAGCCATA‑CCGGTAAAAAATTCAGCACCGTACCGACCACCGATCTGGTGAc > 2:4383232/1‑76 (MQ=255) gTGACATCATCGCGCCGTGGCTCCCCAGCCATA‑CCGGTAAAAAATTCAGCGCCGTACCGACCACCGATCTGGGCAc > 2:3853018/1‑76 (MQ=255) tGACATCATCGCGCCGTGGCTCGCCAGCCATA‑CCGGTAAAAACTTCCGCGCCGTACCGAACACCGATCTGGTCAcc > 2:4559352/1‑76 (MQ=255) gACATCATCGCGCCGTGGCTCGCCAGCCATA‑CCGGTAAAAACTTCAGCGCCGTACCGACCACCGATCTGGGCACCa > 2:417869/1‑76 (MQ=255) tcatcGCGCCGTGGCTCGCCCGCCATA‑CCGGTAAAAACTTCAGCGCCGTACCGACCACCGATCTGGTCACCAATcc > 2:2593295/1‑76 (MQ=255) catcGCGCCGTGGCTCGCCAGCCATA‑CCGGTAAAAACTTCAGCGCCGTACCGACCACCGATCTGGTCACCAATCCg > 1:975253/1‑76 (MQ=255) catcGCGCCGTGGCTCGCCAGCCATA‑ACGGTAAAAACTTCTGCGCCGCACCGACCACCCATCTGGTCACCAAACCg > 2:4445582/1‑76 (MQ=255) cgcgcgGTGCCCCCCCAGCCATA‑CCGGTAAAAACTTCAGCGCCGTACCGACCACCGATCTGGTCACCAATCCGATg < 2:615453/76‑1 (MQ=38) cgcgCCGTGGCTCGCCCGCCCTA‑CCGGTAAAAAATTCAGCGCCGTACCGACCACCGATCTGGTCACCCATCCGATg > 2:7122177/1‑76 (MQ=38) cgcgCCGTGGCTCGCCCGCCATA‑CCGGGAAAAACTTCAGCGCCGTACCGACCACCGATCTGGTCACCCATCCGATg > 1:1008217/1‑76 (MQ=39) ccGTGGCTCGCCAGCCCTA‑CCGGTAAAAACTTCAGCGCCGTACCGACCACCGATCTGGTCACCAATCCGATGGACt > 2:4951326/1‑76 (MQ=255) cGTGGCTCCCCCGCCCTA‑CCGGTAAAAAACTCAGCGCCGTACCGACCACCCATCTGGTCACCAATCCCATGGACTa > 2:2775851/1‑76 (MQ=255) tGGCTCGCCAGCCATA‑CCGGTAAAAACTTCAGCGCCGTACCGACCACCGATCTGGTCACCAATCCGATGGACTAcc > 1:272689/1‑76 (MQ=255) ggCTCGCCAGCCATA‑CCGGTAAAAACTTCAGCGCCGTACCGACCACCGATCTGGTCACCAATCCGATGGACTAccc > 2:7211005/1‑75 (MQ=255) ggCTCGCCAGCCATA‑CCGGTAAAAACTTCAGCGCCGTACCGACCACCGATCTGGTCACCAATCCGATGGACTACCt > 2:5852994/1‑76 (MQ=255) ggCTCGCCAGCCATA‑CCGGTAAAAACTTCAGCGCCGTACCGACCACCGATCTGGTCACCAATCCGATGGACTACCt > 2:5760731/1‑76 (MQ=255) gCTCGCCAGCCATA‑CCGGTAAAAACTTCAGCGCCGTACCGACCACCGATCTGGTCACCAATCCGATGGACTACCTg > 1:4769471/1‑76 (MQ=255) cTCGCCAGCCATA‑CCGGTAAAAACTTCAGCGCCGTACCGACCACCGATCTGGTCACCAATCCGATGGACTACCTGa > 2:1864537/1‑76 (MQ=255) gccagccaTA‑CCGGTAAAAACTTCAGCGCCGTACCGACCACCGATCTGGTCACCAATCCGATGGACTACCTGAAcc > 2:5193273/1‑76 (MQ=255) gccagccaTA‑CCGGTAAAAACTTCAGCGCCGTACCGACCACCGATCTGGTCACCAATCCGATGGACTACCTGAAcc > 2:4234770/1‑76 (MQ=255) gccagccaTA‑CCGGTAAAAACTTCAGCGCCGTACCGACCACCGATCTGGTCACCAATCCGATGGACTACCTGAAcc > 1:6559873/1‑76 (MQ=255) | AGAGAATCTGCGGATCATCCTGACCGGAGCCGGAACGTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCGCCAGCCATA‑CCGGTAAAAACTTCAGCGCCGTACCGACCACCGATCTGGTCACCAATCCGATGGACTACCTGAACC > REL606/3217361‑3217509 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |