| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 433 | 0 | T | G | 40.5% | 129.1 / 32.6 | 84 | V33G (GTG→GGG) | thrA | bifunctional aspartokinase I/homeserine dehydrogenase I |
| Reads supporting (aligned to +/- strand): ref base T (5/45); new base G (34/0); total (39/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.56e-18 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 171 total aligned reads displayed.
GTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGTGGCCACCGTCCT‑CTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATG‑A‑TTGAAAAAACCATTAGCGGCCA > REL606/358‑508 | gTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGt < 2:6615188/76‑1 (MQ=255) tACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGTg < 2:5506749/76‑1 (MQ=255) aCATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGTgg < 1:1373802/76‑1 (MQ=255) cATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGTGGc < 1:6592368/76‑1 (MQ=255) tCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGCTATTCTGGAAAGCAATGCCAGGCA‑GAGGCAGggggggc > 1:5882230/1‑73 (MQ=255) aGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGTGGCCAcc < 2:7198126/76‑1 (MQ=255) gTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGTGGCCACCg < 2:4899985/76‑1 (MQ=255) tGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGGGGGCCCCgc > 2:5105897/1‑75 (MQ=255) tgCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGGGGGGACCgcc > 1:2035926/2‑74 (MQ=255) ggCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGTGGCCACCGTc < 2:1330641/76‑1 (MQ=255) ggCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGTGGCCACCGTc < 2:143173/76‑1 (MQ=255) ggCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGGGGGCCCCggc > 1:5273640/1‑74 (MQ=255) gCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGTGGCCACCGTcc < 1:1168566/76‑1 (MQ=255) gCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGGGGGCACCGTcc > 1:504336/1‑76 (MQ=255) cAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGGGGGCACCCTccc > 2:2380607/1‑75 (MQ=255) aaaTGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGGGGGCACCGTCct‑c > 2:7011812/1‑76 (MQ=255) aaTGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGTGGCCACCGTCct‑ct < 1:3631341/76‑1 (MQ=255) aTGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGTGGCCACCGTCct‑ctc < 2:72111/76‑1 (MQ=255) tGCAGAACTTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGTGGCCACCGTCct‑ctct < 2:4768081/76‑1 (MQ=255) tGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGTGGCCACCGTCct‑ctct < 1:5533190/76‑1 (MQ=255) tGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGTGGCCACCGTCct‑ctct < 2:2616985/76‑1 (MQ=255) tGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGTGGCCACCGTCct‑ctct < 2:6843465/76‑1 (MQ=255) tGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGTGGCCACCGTCct‑ctct < 1:7482796/76‑1 (MQ=255) tGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGGGGCCCCCGGCct‑ttcc > 1:1278068/1‑75 (MQ=255) gCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGTGGCCACCGTCCT‑CTCTg < 1:4716806/76‑1 (MQ=255) gCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGTGGCCACCGTCCT‑CTCTg < 2:6048005/76‑1 (MQ=255) cAGACCGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGTGGCCACCGTCCT‑CTCTGc < 1:4393001/76‑1 (MQ=255) cAGAACTTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGTGGCCACCGTCCT‑CTCTGc < 2:3923855/76‑1 (MQ=255) gAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGGGGCCCCCCGCCC‑CTCTcccc > 1:2063614/1‑76 (MQ=255) aaCGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGTGGCCACCGTCCT‑CTCTGcccc < 2:390426/76‑1 (MQ=255) aaCGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGGGGGCCCCGGCCT‑CCCTTcccc > 1:1554335/1‑76 (MQ=255) aCGTTTTCTGCGGTTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGTGGCCACCGTCCT‑CTCTGccccc < 1:4562617/76‑1 (MQ=255) aCGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGTGGCCACCGTCCT‑CTCTGccccc < 2:680628/76‑1 (MQ=255) cGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGTGGCCACCGTCCT‑CTCTGCCCCCg < 1:2727370/76‑1 (MQ=255) cGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGTGGCCACCGTCCT‑CTCTGCCCCCg < 2:5824107/76‑1 (MQ=255) ttttCTGCGGGTTGCCGATATTCTGGAAAGCAATGACACGCA‑GGGGCAGGGGGGCCCCCTCCG‑CTTCGGCCCCGcc > 2:452569/1‑76 (MQ=255) tttGTGGGGTTTGCCGATATTCTGAAAAGCAATGCCAGGAG‑GGGGCAGGTGGCCACCGTCCT‑CTCTGCCCCCGCCa < 2:5003001/76‑1 (MQ=255) tttCGGGGGTTTGCCAATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGTGGCCCCCGTCCT‑CTCTGCCCCCGCCa < 1:6116841/76‑1 (MQ=255) ttCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGGGGGCACCGTCCT‑CTCTCccccccccca > 1:1134522/1‑74 (MQ=255) cTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGTGGGCACCGGCCT‑CTCTCCCCCCCCCcaaa > 1:1541143/1‑76 (MQ=255) cTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGGGGGCCCCGTCCC‑CTCTGCCCCCCCCcaaa > 2:6321331/1‑76 (MQ=255) cTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGGGGGCACCGGCCC‑CTCTGCCCCCCCCcaaa > 2:491609/1‑76 (MQ=255) tGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGGGGGCACCCTCCTCCTCTCCCCCCCCCcaaa > 2:4058219/1‑76 (MQ=255) cGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGGGGGACGCGGCCC‑CTCTGCCCCCGCCCAAAACa > 2:4310081/1‑76 (MQ=255) ttGCCGATATTCTGGAAAGCAATGCCGGGCG‑GGGGCAGGTGGCCACCGTCCT‑CTCTGCCCCCGCCAAAATCaccaa < 2:2748613/76‑1 (MQ=255) tGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGGGGGCACCGTCCT‑CCCTTCCCCCCCCCAAAACacccaa > 1:6919614/1‑73 (MQ=255) tGCCGATATTCGGAAAAGCAATGCCGGGCA‑GGGGCAGGTGGCCCCCGTCCT‑CTCTGCCCCCGCCAAAATCaccaac < 1:2643103/76‑1 (MQ=255) tGCCAATTTTCTGAAAAGCAATGCCGGGCG‑GGGGCAGGTGGCCCCCGTCCT‑CTCTGCCCCCGCCAAAATCaccaac < 1:4423902/76‑1 (MQ=255) gCCGATTTTTGGAAAAGAAATGCCGGGCG‑GGGGCGGGTGGCCACCGTCCT‑CTCGGCCCCCGCCAAAATCaccaacc < 2:4421700/76‑1 (MQ=255) ccGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGGGGCCACCCCCCC‑CCCTGCCCCCCCCAAAAACaccaaccc > 1:885214/1‑75 (MQ=255) atatTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGGGGCCACCGTCCT‑CTCTTCCCCCGCCCAAAACACCAacccccc > 1:3830875/1‑75 (MQ=255) tatTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGTGGCCCCCGTCCT‑CTCTGCCCCCGCCAAAATCACCAACCACCtg < 2:1940664/76‑1 (MQ=255) tatTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGTGGAAACCGGGCT‑CTCTCCCCCCCCCCCAAACAACAagcaccaa > 1:5423493/1‑74 (MQ=255) tatTCGGAAAACCAAGCCCGGGCG‑GGGGCAGGTGGCCCCCGTCCT‑CTCTGCCCCCGCCAAAATCACCAACCACCtg < 1:7242525/76‑1 (MQ=255) atTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGTGGGCACCCTCCC‑CCCTTCCCCCCCCAAAAGCACCAAACACCcgg > 2:3452136/1‑76 (MQ=255) atTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGGGGGCACCGCCCC‑CTCCTCCCCCGCCCACATAACCCAACACCtcg > 2:6491298/1‑74 (MQ=255) atTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGGGGCCCCCGTGCT‑CTCCTCCCCCGCCCAAACCAACACACACCtgg > 1:1058078/1‑76 (MQ=255) ttCTGAAAAGAAATGCCGGGCA‑GGGGCGGGTGCCCCCCGTCCT‑CTCTGCCCCCGCCAAAATCACCAACCACCtggt < 2:2730420/76‑1 (MQ=255) tgttGGAAAACAATGCAAGACG‑GGGGCGGGTGGCCCCCGTCCT‑CTCGGCCCCCGCCAAAATCACCAACCACCtggt < 2:7436820/73‑1 (MQ=255) tccTGGAGAGCAA‑GCCGGGGAGGGGGCAGGTGCCCCCCGTCCC‑CCCTCCCCCCGCCAAAATCACCAACCACCtggt < 1:5293525/74‑1 (MQ=255) tGGAAAGCAATGCCAGGCA‑GGGGCAGGGGGGCACCCCCCT‑CTCTGTCCCCGCCAAAATCACCCACCACCTGGGGGc > 2:5151775/1‑76 (MQ=255) gggAAAGAAAGCCCAGGCA‑GGGGCAGGTGACCACCGTCCT‑CTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGc < 1:1978685/75‑1 (MQ=255) ggAAAGCAATGCCAGGCA‑GGGGCAGGGGGCCACCGGCCT‑CTCTGCCCCCCCCAAAATCACCAACCACCCGGGGGCg > 1:7659150/1‑76 (MQ=255) gAAAGCAATGCCAGGCA‑GGGGCAGGGGGGGCCCCGCCT‑CTCTGCCCCCCCCCAAAACACCAACCCCCCGGGGGCgg > 2:732307/1‑75 (MQ=255) aaggCAATGCCAGGCA‑GGGGCGGGTGGCCCCCGTCCT‑CTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCgat < 1:4195860/73‑1 (MQ=255) aagcaagcCCAGGCA‑GGGGCGGGTGCCCCCCGTCCT‑CTCTCCCCCCGCCAAAATCACCAACCACCTGGTGGCgatg < 2:1621186/76‑1 (MQ=255) aaGCAATGCCAGGCG‑GGGGGGGGTGCCCCCCGCCCT‑CTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCgatg < 2:2078988/76‑1 (MQ=255) aaGCAATGCCAGGCA‑GGGGCAGGGGGCCACCGTCCT‑CTCTTCCCCCGCCCAAATCACCAACCACCCGGTGGCgatg > 1:4164253/1‑76 (MQ=255) cAATGCCAGGCA‑GGGGCAGGGGGGCACCGTCCT‑CTCTGCCCCCGCCCAAAACACCAACCAACTGGGGGCGGTG‑A‑tt > 2:6274510/1‑76 (MQ=255) cAATGCCAGGCA‑GGGGCAGGGGGGCACCGTCCC‑CTCTGCCCCCCCCCAAAACACCAAACAACCGGTGGCgatgcat > 2:966712/1‑73 (MQ=255) cAATGCCAGGCA‑GGGGCAGGGGGGCACCCTCCT‑CTCTGCCCCCCCCCAAAACACCAACCACCTGGTGGCGAGG‑A‑tt > 2:5549778/1‑76 (MQ=255) cAATGCCAGGCA‑GGGGCAGGGGGCCACCGCCCC‑CTCTCCCCCCGCCCAAAACACCAACACCCCGGTGGCGAGG‑A‑tt > 1:7259446/1‑76 (MQ=255) cAATGCCAGGCA‑GGGGCAGGGGGACCCCCTCCT‑CTCTGCCCCCGCCCAAATAACCAACCAACTGGCGGCGCTG‑G‑tt > 2:1168117/1‑76 (MQ=255) agtGCGAGGCA‑GGGGCAGGTGGCCACCTTCCT‑CTCCGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATG‑A‑TTg < 2:2315741/74‑1 (MQ=255) aaTGCCAGGCA‑GGGGCAGGGGGGCCCCCGCCC‑CCCTTCCCCCCCCCAAAACACCAACCACCTGGTGGCGGTG‑A‑ttt > 2:2900929/1‑75 (MQ=255) aaTGCCAGGCA‑GGGGCAGGGGGCCACCGTCCC‑CTCTTCCCCCCCCAAAATCACCAACCACCTGGTGGGCATG‑A‑TTg > 2:5302191/1‑76 (MQ=255) acgCCAGGCG‑GGGGCGGGGGCCCCCCGCCCT‑CTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATG‑A‑TTGa < 1:605177/74‑1 (MQ=255) tGCCGGGCG‑GGGGGAGTGGCCCCCCGTCCT‑CTCGGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATG‑A‑TTGaa < 1:926760/76‑1 (MQ=255) gCCAGGCG‑GGGGCGGGTGCCCCCCGTCCT‑CTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATG‑A‑TTGaaa < 1:4668679/76‑1 (MQ=255) caggca‑ggGGCGGGTGCCCACCGTCCT‑CTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATG‑A‑TTGaaaaa < 1:1844768/76‑1 (MQ=255) caggca‑ggGGCAGGGGGGCACCGTCCT‑CTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATG‑A‑TTGaaaaa > 2:3746326/1‑76 (MQ=255) caggca‑ggGGCAGGGGGCCACCGCCCC‑CTCTGCCCCCCCCCAAATTACCCACTACCTCGGGGCGATG‑ACTTGaaaa > 2:573100/1‑76 (MQ=255) aggca‑ggGGCAGGGGGGCACCGTCCT‑CTCTTCCCCCGCCAAAATAACCAAACACCTGGTGGCGATG‑A‑TTGaaaaaa > 2:7656176/1‑76 (MQ=255) aggca‑ggGGCAGGGGGGCACCGTCCT‑CTCTGCCCCCCCCCAAATCACCCACCACCTGGTGGCGGTGAA‑TTGaaaaa > 2:2548936/1‑76 (MQ=255) aggca‑ggGGCAGGGGGGCACCGACCT‑CTCTGCCCCCGCCAAAATAACAAACCACCTGGTGGCGGCG‑A‑GTGaaaaaa > 2:3479159/1‑76 (MQ=255) aggca‑ggGGCAGGGGGCCCCCGCCCC‑CTCTTCCCCCCCCCAAAACACCAAACACCGGGGGGCGATG‑A‑TTTgaaaaa > 2:881157/1‑76 (MQ=255) gggggggcgggGGCCCCCCTTCCT‑CTCTCCCCCCGCCAAAATCACCAACCACCTGGTGGCGATG‑A‑TTGAAAAAAcc < 2:6139138/73‑1 (MQ=255) gcgggggcgggTGCCCCCCGTCCT‑CTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATG‑A‑TTGAAAAAAcc < 1:5395470/73‑1 (MQ=255) gcgggggcgggTGCCCCCCGCCCT‑CTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATG‑A‑TTGAAAAAAcc < 2:4393859/73‑1 (MQ=255) gagggggcgggGGGCCCCCGTCCT‑CTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATG‑A‑TTGAAAAAAcc < 1:2214806/73‑1 (MQ=255) a‑ggggcaggGGGGCCCCGTCCT‑CTCTGCCCCCCCCCAAAACACCAACCACCTCGGGGCGGTG‑A‑TTGGAAAAAACaa > 2:5368672/1‑75 (MQ=255) ggggcggtTGCCCCCCGTCCT‑CTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATG‑A‑TTGAAAAAACCAtt < 1:1268003/76‑1 (MQ=255) gggcaggTGGCCACCGTCCT‑CTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATG‑A‑TTGAAAAAACCATTa > 2:4021406/1‑76 (MQ=255) gccaggTGTCCGCCGTCCT‑CTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATG‑A‑TTGAAAAAACCATTAg < 2:5648436/74‑1 (MQ=255) aggTGGCCACCGTCCT‑CTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATG‑A‑TTGAAAAAACCATTAGCgg > 2:3972177/1‑76 (MQ=255) aggTGGCCACCGTCCT‑CTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATG‑A‑TTGAAAAAACCATTAGCgg > 1:2530060/1‑76 (MQ=255) aggTGGCCACCGTCCT‑CTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATG‑A‑TTGAAAAAACCATTAGCgg > 2:4070764/1‑76 (MQ=255) gTGGCCCCCGCCCT‑CTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATG‑A‑TTGAAAAAACCATTAGCGGcc < 2:1840783/76‑1 (MQ=255) gTGGCCACCGTCCT‑CTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATG‑A‑TTGAAAAAACCATTAGCGGcc > 1:2864431/1‑76 (MQ=255) gTGCCCCCCGTCCT‑CTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATG‑A‑TTGAAAAAACCATTAGCGGcc < 1:897947/76‑1 (MQ=255) | GTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCA‑GGGGCAGGTGGCCACCGTCCT‑CTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATG‑A‑TTGAAAAAACCATTAGCGGCCA > REL606/358‑508 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |