| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,472,700 | 0 | C | G | 43.9% | 32.6 / 73.2 | 66 | A290G (GCG→GGG) | yhgA | predicted transposase |
| Reads supporting (aligned to +/- strand): ref base C (31/6); new base G (0/29); total (31/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.98e-13 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 134 total aligned reads displayed.
TAATGCTTGAATCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCA‑GTAAATTTCTGTCTCGCC‑ATTTCAAAAGTTGCCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAA > REL606/3472625‑3472775 | taatGCTTGAATCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTggggc > 2:6536363/1‑76 (MQ=255)taatGCTTGAATCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTgcggc > 1:3377304/1‑76 (MQ=255) aatGCTTGAATCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTgcggcg < 1:1846522/76‑1 (MQ=255) aatGCTTGAATCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTgcggcg < 1:6298135/76‑1 (MQ=255) tGCTTGAATCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTgcggcggg > 1:2281824/1‑74 (MQ=255) tGCTTGAATCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTgcggcggg > 1:5957292/1‑74 (MQ=255) gCTTGAATCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGc > 1:7685244/1‑76 (MQ=255) cTTGAATCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGcc > 2:5767081/1‑76 (MQ=255) cTTGAATCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGcc > 1:7380512/1‑76 (MQ=255) cTTGAATCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGcc < 2:2992384/76‑1 (MQ=255) ttGAATCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCa < 2:2118557/76‑1 (MQ=255) ttGAATCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCa > 2:6073568/1‑76 (MQ=255) ttGAATCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCa < 1:7347367/76‑1 (MQ=255) gAATCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGGCA‑Gt > 1:5482321/1‑76 (MQ=255) aaTCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGGCA‑gga > 1:5077642/1‑74 (MQ=255) aaTCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCA‑GTa > 1:3047730/1‑76 (MQ=255) aTCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGACGAAGAGTTGGCTGCGGCGAGGCG‑GTaa > 2:728110/1‑76 (MQ=255) tCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGGGGGGAGCCA‑GCaaa > 2:1589218/1‑76 (MQ=255) tCCGGACCCCCTCTTACAGACATCATGCGCTTTACGGGGGTGTAAGAAGAAGAGTGGGCGGGGGGGAGCCA‑GTaaa < 2:1336292/76‑1 (MQ=255) cGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCA‑GGAAAtt > 1:6252477/1‑76 (MQ=255) ggAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGGCA‑GGAAAatt > 1:6460254/1‑76 (MQ=255) aGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCA‑GGAAAttttt > 2:4089173/1‑74 (MQ=255) aGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCA‑GGAAATTTCt > 2:7178512/1‑76 (MQ=255) aGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAAAAGGGTTGGCTGCGGGGAGCCA‑GTAAATTTCt < 2:1993481/76‑1 (MQ=255) tCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGCGGCA‑GGAAAATTCTgc > 1:426960/1‑75 (MQ=255) cccTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGGCA‑GGAAATTTCTGtc > 1:5758441/1‑76 (MQ=255) ccTCTTACAGACATCAGGCGCTTTCCGGGGGTATGAGAAGAAGAGTGGGCGGGGGGGAGCCA‑GTAAATTTCTGtct < 2:4956333/76‑1 (MQ=255) ctctTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCC‑GTAAATTTCTGtctc > 1:766197/1‑76 (MQ=255) ctctTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCA‑GTGAATTTCTTtctc > 1:5628932/1‑76 (MQ=255) tctTACAGACATCATGCGCTTTCCGGGGGTGTCAGAAGAAGATGGGGCGGCGGGGAGCCA‑GTAAATTTCTGTCTCg < 2:1458909/76‑1 (MQ=255) tctTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCC‑GTAAAATTTTGTCTCg > 2:1984045/1‑76 (MQ=255) tctTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCA‑GTAAAATTCTGtctcc > 1:6793930/1‑75 (MQ=255) tctTACAGACACCAGGCGTTTTGCCGGGGTGTAAAAAGAAGAGTGGGCTGCGGGGAGCCA‑GTAAATTTCTGTCTCg < 1:1036094/76‑1 (MQ=255) tctTAAAGAGATGATGCGCTTCACGGGGGTGTAAAAAAAAGAGTTGGCGGCGGGGAGCCA‑GTAAATTTCTGTCTCg < 1:5468507/76‑1 (MQ=255) ctTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGTGCCA‑GTAAATTTCTGTCTCGc > 1:7786278/1‑76 (MQ=255) ctTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCA‑GTAAATTTCTGTCTCGc > 1:5279243/1‑76 (MQ=255) ctTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCA‑GTAAATTTCTGTCTCGc > 1:5675555/1‑76 (MQ=255) ctTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCA‑GCAAATTTTTGGTTCGc > 1:4952924/1‑76 (MQ=255) ttACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGGGGCGAGCCA‑GTAAATTTTTGTCTCGcc > 2:1690740/1‑76 (MQ=255) aCAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCA‑GTAAATTTCTGTCTCGccct > 2:4954165/1‑74 (MQ=255) cAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCA‑GTAAATTTCTGTCTCGCC‑Att > 1:3324125/1‑76 (MQ=255) aGACATCATGCGGTTTCCGGGGGTGTAAGAAGAAGGGTGGGCTGCGGGGAGCCA‑GTAAATTTCTGTCTCGCC‑Attt < 1:7560214/76‑1 (MQ=255) aGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAACCA‑GGAAATTTTTGGATCGCC‑Attt > 2:6196405/1‑76 (MQ=255) aGACACCAGGCGCTGTCCGGGGGTGGAAGAAAGAGGGTGGGTTGGGGCGAGCCA‑GTAAATTTCTGTCTCGCC‑Attt < 2:2645731/76‑1 (MQ=255) gACATCTTGCGCTTTACGGGGGTGTAAGAGGAGGGGTTGGCGGGGGGGAGCCA‑GTAAATTTCTGTCTCGCC‑ATTTc < 1:5655645/76‑1 (MQ=255) caccatGCGTTTTCCGGGGGTGTAAAAAGAAGAGTGGGCTGCGGGGAGCCA‑GTAAATTTCTGTCTCGCC‑ATTTCaa < 1:2385941/73‑1 (MQ=255) tcatGCGTTTTCCCGGGGTGAAAAAAGAGGGGGGGGCGGCGGGGAGCCA‑GTAAATTTCTGTCTCGCC‑ATTTCaaaa < 2:4095343/76‑1 (MQ=255) tcatGCGCTTTCCGGGGGTGTAAGAAGAAGGGTTGGCGGCGGGGAGCCA‑GTAAATTTCTGTCTCGCC‑ATTTCaaaa < 2:7750476/76‑1 (MQ=255) tcatGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCA‑GTCAATTTCTGTCTCGCC‑CTTTCaaaa > 2:7557026/1‑76 (MQ=255) tcatGCGCTTTACCGGGGTGTAAGAAGAAGAGTTGGCTGCGGCGAGCCA‑GTAAATTTCTGTCTCGCC‑CTTTCcaaa > 1:1826194/1‑76 (MQ=255) tcagGCGCTTTGCGGGGGTGTCAGAAGAGGGGTGGGCGGCGGGGAGCCA‑GTAAATTTCTGTCTCGCC‑ATTTCaaaa < 1:2323295/76‑1 (MQ=255) catGCTTTTTCCGGGGGTGTAAGAAGAAGGGTGGGCTGCGGGGAGCCA‑GTAAATTTCTGTCTCGCC‑ATTTCAAAAg < 1:107395/76‑1 (MQ=255) catGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGGGCCA‑GTCAATTTCTTTCTCGCC‑CTATCcaaac > 2:436777/1‑75 (MQ=255) catGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCA‑GTAAATTTCTGTCTCGCC‑ATTTCAAAAg > 1:1067024/1‑76 (MQ=255) catGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCA‑GGAAATTTCTGTCTCGCC‑CTTTCAAAAg > 1:2424475/1‑76 (MQ=255) catGCGCTGGAGCGGGGTGAGAGAAAAAGGGTGGGCGGCGGGGAGCCA‑GTAAATTTCTGTCTCGCC‑ATTTCAAAAg < 1:4960267/76‑1 (MQ=255) atGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGGGCCA‑GGAAAATTTTGTTTCGCC‑CTTTTaaaaat > 1:4355323/1‑74 (MQ=255) tGCGCTTTCCCGGGGTGTCAGAAGAAGAGTGGGCTGCGGGGAGCCA‑GTAAATTTCTGTCTCGCC‑ATTTCAAAAGtt < 2:5832221/76‑1 (MQ=255) tGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGGGGCGAGCCA‑GTAAATTTCTGTCTCGCC‑ATTTCAAAAGtt > 2:6965604/1‑76 (MQ=255) tGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGGCAGGAAAAATTTTGT‑TCGCCAATTTCAAAAGt > 1:2011533/1‑76 (MQ=255) cTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGCGCCC‑GGAAATTTCTGTCTCCCC‑CTTTTCAAAATTTCCt > 1:4426448/1‑76 (MQ=255) cTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCC‑GCAAAATTTTGTCTCCCC‑CATTCAAAAATTGctc > 2:3141648/1‑74 (MQ=255) cTTTACCGGGGTGTCAGAAGAAAAGTTGGCTGCGGCGAGCCA‑GTAAATTTCTGTCTCGCC‑ATTTCAAAAGTTGCCt > 2:1252278/1‑76 (MQ=255) tttACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCA‑GTAAATTTCTGTCTCGCC‑CTTTCAAAAGTTGCCTa > 1:5373881/1‑76 (MQ=255) tACGGGGGTGACAAAAGAGGAGTTGGCGGGGGGGAGCCA‑GTAAATTTCTGTCTCGCC‑ATTTCAAAAGTTGCCTaca < 2:1575714/76‑1 (MQ=255) gggggggTGTAAAAAGAGGGGTGGGCGGGGGGGAGCCA‑GTAAATTTCTGTCTCGCC‑ATTTCAAAAGTTGCCTacac < 2:7115921/73‑1 (MQ=255) aggggggTGAAAGAAAAAGAGGGGGCGGGGGGGAGCCA‑GTAAATTTCTGTCTCGCC‑ATTTCAAAAGTTGCCTacac < 1:5035130/73‑1 (MQ=255) acgggggTGAAAAAAGAAGGGGGGGCGGGGGGGAGCCA‑GTAAATTTCTGTCTCGCC‑ATTTCAAAAGTTGCCTacac < 1:240535/73‑1 (MQ=255) aCCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCA‑GTAAATTTTTGTCTCCCC‑CTTTCCAAAATTGCCTccac > 1:2903125/1‑76 (MQ=255) aCCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCA‑GTAAATTTCTGTCTCGCC‑ATTTCAAAAGTTGCCTacac > 2:3424943/1‑76 (MQ=255) cgggggTGTAAGAAGAGGAGTGGGCGGGGGGGAGCCA‑GTAAATTTCTGTCTCGCC‑ATTTCAAAAGTTGCCTACAcc < 2:5759967/74‑1 (MQ=255) ccGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCA‑GTAAATTTTTCCCTCGGC‑AATTCCAAAATTGGCTAAAcc > 1:5273240/1‑76 (MQ=255) ccGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCA‑GTAAAATTCTTTCTCCCC‑CCTTCAAAAATTTCCCACAcc > 1:6255563/1‑76 (MQ=255) cGGGGTGTCAGAAGAAGAGTTGGCTGCGGGGAGCCC‑GGAAATTTCTGTCTCGCC‑ATTTCAAAAGGTGCCTCCAccc > 1:1406283/1‑76 (MQ=255) ggggTGAAAGAAGAAGGTGTGGCTGCGGGGAGCCA‑GTAAATTTCTGTCTCGCC‑ATTTCAAAAGTTGCCTACACCct < 2:6843085/76‑1 (MQ=255) ggggGTCAGAAGAAGAGTGGGCGGCGGGGAGCCA‑GTAAATTTCTGTCTCGCC‑ATTTCAAAAGTTGCCTACACCctc < 1:3452586/76‑1 (MQ=255) ggTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCC‑GTAAATTTCTGTCTCGCC‑ATTTCAAAAGTTGCCTACACCctct > 2:6370348/1‑76 (MQ=255) ggTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCA‑GTAAAATTCTTTCTCGCC‑ATTTCAAAAAGTGCCTACACCctct > 1:4964652/1‑76 (MQ=255) ggTGTAAAAAGAAGTGTTGGCGGCGGGGAGCCA‑GTAAATTTCTGTCTCGCC‑ATTTCAAAAGTTGCCTACACCctct < 2:504464/76‑1 (MQ=255) tgtCAGAAGAAGAGTTGGCTGCGGCGAGGCC‑GGAAAATTCTGTCTCTCC‑CTTTCAAAAATTGCCCACAACCCCTGc > 1:4922400/1‑76 (MQ=255) tCAGAAGAAGAGTTGGCTGCGGCGAGCCA‑GTAAATTTCTGTCTCGCC‑CTTTTAAAAGGTGCCTACACCCTCTGCtt > 2:6285140/1‑76 (MQ=255) cAGAAGAAGAGTTGGCTGCGGCGAGCCA‑GTAAATTTCTGTCTCTCC‑ATTTTAAAAGATGCCTACCACCTCCTCTTc > 1:1252031/1‑76 (MQ=255) aagaagaGTTGGCTGCGGCGAGCCA‑GTAAATTTCTGTCTCGCC‑ATTTCAAAAGTTGCCTACACCCTCTGCTTCAAc > 1:5826848/1‑76 (MQ=255) aagaagaGTTGGCTGCGGCGAACCA‑GTAAATTTTTGTCTCGCC‑CTTTCAAAAGGTGGCTACAACCTCTGCTTCCAc > 2:239328/1‑76 (MQ=255) agaaggGTGGGCGGGGGGGAGCCA‑GTAAATTTCTGTCTCGCC‑ATTTCAAAAGTTGCCTACACCCTCTGCTTCAACg < 1:3071842/76‑1 (MQ=255) gaagaGTTGGCTGCGGCGAGCCA‑GTAAATTTCTGTCTCGCC‑ATTTCCAAAGTTGCCTACACCCTCTGCTTCAACGc > 1:5511470/1‑76 (MQ=255) aaggGTTGGCGGGGGGGAGCCA‑GTAAATTTCTGTCTCGCC‑ATTTCAAAAGTTGCCTACACCCTCTGCTTCAACGcc < 1:2496310/76‑1 (MQ=255) aagaGTTGGCTGCGGCGAGCCA‑GTAAATTTCTGTCTCGCC‑ATTTTAAAAGTTGCCTACACCCTCTGCTTTCACGcc > 2:6321421/1‑76 (MQ=255) aagaGTTGGCTGCCGCGAGTCA‑GAAAAATTCTATATCGCC‑CATTTTAAAGTTGCCTAAAACCCCTCCGTCTACGcc > 2:2562263/1‑76 (MQ=255) gagTTGGCTGCGGCGAGCCA‑GTAAATTTCTGTCTCGCC‑ATTTCAAAAGTTGCCTACCACCTCTGCTTCCACGcccc > 1:986753/1‑74 (MQ=255) tgtGGCCGCGGGGAGCCA‑GTAAATTTCTGTCTCGCC‑ATTTCAAAAGTTGCCTACACCCTCTGCTTCAACGccacca < 1:1375016/74‑1 (MQ=255) ggtGGCGGGGGGGAGCCA‑GTAAATTTCTGTCTCGCC‑ATTTCAAAAGTTGCCTACACCCTCTGCTTCAACGccacca < 1:6444992/74‑1 (MQ=255) gggggCTGGGGGGAGCCA‑GTAAATTTCTGTCTCGCC‑ATTTCAAAAGTTGCCTACACCCTCTGCTTCAACGccacca < 2:457931/73‑1 (MQ=255) gTTGGCTGGGGGGAGCCA‑GTAAATTTCTGTCTCGCC‑ATTTCAAAAGTTGCCTACACCCTCTGCTTCAACGccacca < 2:7052950/76‑1 (MQ=255) ggcggcGGGGAGCCA‑GTAAATTTCTGTCTCGCC‑ATTTCAAAAGTTGCCTACACCCTCTGCTTCAACGCCACcagca < 2:7362910/76‑1 (MQ=255) cTGCGGCGAGCCA‑GTAAATTTCTGTCTCGCC‑ATTTCAAAAGTTGCCTACACCCTCTGCTTCAACGCCACCAGCAgg > 2:1007210/1‑76 (MQ=255) cTGCGGCGAGCCA‑GTAAATTTCTGTCTCGCC‑ATTTCAAAAGTTGCCTACACCCTCTGCTTCAACGCCACCAGCAgg > 2:7237121/1‑76 (MQ=255) tGCGGCGAGCCA‑GTAAATTTCTGTCTCGCC‑ATTTCAAAAGTTGCCTAAACCCTCTGCTTAAACGCCACCAGCAggc > 2:4340616/1‑75 (MQ=255) cgAGCCA‑GTAAATTTCTGTCTCGCC‑ATTTCAAAAGTTGCCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACaa > 2:7380055/1‑76 (MQ=255) cgAGCCA‑GTAAATTTCTGTCTCGCC‑ATTTCAAAAGTTGCCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACaa > 2:286790/1‑76 (MQ=255) | TAATGCTTGAATCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCA‑GTAAATTTCTGTCTCGCC‑ATTTCAAAAGTTGCCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAA > REL606/3472625‑3472775 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |