BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,015,124	G→A	G182D (GGC→GAC)	ECB_02816 →	KpsD protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,015,124	0	G	A	99.0%	1200.5 / 1.2	290	G182D (GGC→GAC)	ECB_02816	KpsD protein
Reads supporting (aligned to +/- strand): ref base G (1/2); new base A (131/156); total (132/158)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.66e-01

Only 100 of 291 total aligned reads displayed.

TACGCCTCCTTATTACAGGCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGGCGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCGGAAGTTA  >  REL606/3015051‑3015196

tACGCCTCCTTATTACAGGCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACg                                                                        <  1:2806644/76‑1 (MQ=255)

tACGCCTCCTTATTACAGGCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACg                                                                        >  1:4306794/1‑76 (MQ=255)

 aCGCCTCCTTATTACAGGCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACgg                                                                       >  1:1036537/1‑76 (MQ=255)

   gCCTCCTTATTACAGGCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGgtg                                                                     >  2:3723898/1‑76 (MQ=255)

    cctcctTATTCCGGGCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGgtgt                                                                    <  1:3411755/76‑1 (MQ=255)

    cctcctTATTACAGGCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGgtgt                                                                    >  1:6729964/1‑76 (MQ=255)

     ctcctTATTACAGGCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGtgtg                                                                   <  1:3554069/76‑1 (MQ=255)

      tcctTATTACAGGCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGa                                                                  <  1:7771800/76‑1 (MQ=255)

       cctTATTACAGGCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGAc                                                                 >  2:7672115/1‑76 (MQ=255)

         ttattaCAGGCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGt                                                               >  2:2181138/1‑76 (MQ=255)

          tattaCAGGCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTc                                                              >  2:6529247/1‑76 (MQ=255)

          tattaCAGGCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTc                                                              >  2:5015754/1‑76 (MQ=255)

          tattaCAGGCCCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTc                                                              <  1:7628796/76‑1 (MQ=255)

           attaCAGGCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCt                                                             >  2:7584081/1‑76 (MQ=255)

           attaCAGGCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCt                                                             >  2:201158/1‑76 (MQ=255)

            ttaCGGGCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTg                                                            <  2:6355120/76‑1 (MQ=255)

            ttaCATGCGCAGCCAGGAAAAGTGTACGAGACCGGATTTGTGCGGCATCCTGGTCTGTATGACGGAGTGACGTCTg                                                            >  2:3217483/1‑76 (MQ=255)

            ttaCAGGCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTg                                                            <  2:4749286/76‑1 (MQ=255)

               cAGCCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGAtt                                                         <  1:5320342/76‑1 (MQ=255)

                aGGCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTc                                                        <  1:2008224/76‑1 (MQ=255)

                    gcAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTa                                                    >  1:1629546/1‑76 (MQ=255)

                    ccAGCCAGTAAAAGTGTACGTGTCCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTa                                                    <  1:3123917/75‑1 (MQ=255)

                     cAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTAc                                                   >  1:4470158/1‑76 (MQ=255)

                       gCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTc                                                 >  1:5946338/1‑76 (MQ=255)

                        ccAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCa                                                >  1:6863611/1‑76 (MQ=255)

                        ccAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCa                                                <  1:7558209/76‑1 (MQ=255)

                        ccAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCa                                                <  1:1141918/76‑1 (MQ=255)

                         cAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCaa                                               <  1:1564822/76‑1 (MQ=255)

                          aGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAAt                                              >  2:6239482/1‑76 (MQ=255)

                           gTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAAtt                                             <  2:5215605/76‑1 (MQ=255)

                            tAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTa                                            >  2:3462080/1‑76 (MQ=255)

                             aaaaGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTAt                                           >  1:6342260/1‑76 (MQ=255)

                             aaaaGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTAt                                           >  1:4584962/1‑76 (MQ=255)

                             aaaaGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTAt                                           >  2:2774627/1‑76 (MQ=255)

                             aaaaGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTAt                                           <  2:4954913/76‑1 (MQ=255)

                                  tgtACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGAt                                      <  2:2631896/76‑1 (MQ=255)

                                  tgtACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGAt                                      <  2:4037615/76‑1 (MQ=255)

                                  tgtACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGAt                                      >  2:956802/1‑76 (MQ=255)

                                   gtACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATc                                     >  2:2439346/1‑76 (MQ=255)

                                   gtACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATc                                     >  1:2594442/1‑76 (MQ=255)

                                   gtACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATc                                     >  2:7465476/1‑76 (MQ=255)

                                   gtACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATc                                     <  2:7008335/76‑1 (MQ=255)

                                   gtACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATc                                     >  2:6888150/1‑76 (MQ=255)

                                    tACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCa                                    <  1:6358134/76‑1 (MQ=255)

                                    tACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCa                                    >  1:2216842/1‑76 (MQ=255)

                                    tACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCa                                    <  1:574883/76‑1 (MQ=255)

                                     aCGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCATTACTCAATTATCTGATCaa                                   <  1:5011216/76‑1 (MQ=255)

                                      cGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAg                                  <  1:7181447/76‑1 (MQ=255)

                                      cGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAg                                  <  2:835667/76‑1 (MQ=255)

                                        tGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGc                                >  1:2972611/1‑76 (MQ=255)

                                        tGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGc                                >  2:3844604/1‑76 (MQ=255)

                                        tGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGc                                >  2:3126139/1‑76 (MQ=255)

                                        tGACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGc                                <  1:4055618/76‑1 (MQ=255)

                                         gACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCt                               >  1:5672150/1‑76 (MQ=255)

                                         gACCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCt                               <  2:1284331/76‑1 (MQ=255)

                                          aCCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTg                              <  2:7879716/76‑1 (MQ=255)

                                          aCCGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTg                              >  2:4366367/1‑76 (MQ=255)

                                           ccGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTgg                             >  1:1420573/1‑76 (MQ=255)

                                            cGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTggg                            >  2:795077/1‑75 (MQ=255)

                                            cGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTggg                            >  2:4624546/1‑75 (MQ=255)

                                            cGGATTTGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTggc                            <  1:232742/76‑1 (MQ=255)

                                                tttGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTggcgggg                        >  1:5788071/1‑74 (MQ=255)

                                                tttGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGgcggcg                        >  1:196322/1‑76 (MQ=255)

                                                 ttGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTgggggggg                       >  2:5497927/1‑73 (MQ=255)

                                                 ttGTGCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGt                       >  2:7502137/1‑76 (MQ=255)

                                                   gtgCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTg                     <  2:1385590/76‑1 (MQ=255)

                                                    tgCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGa                    <  2:6041029/76‑1 (MQ=255)

                                                     gCGTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGAt                   >  1:5592201/1‑76 (MQ=255)

                                                       gTAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATcc                 >  1:3944908/1‑76 (MQ=255)

                                                        tAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGGGGGGTTGATTCa                >  1:6627696/1‑76 (MQ=255)

                                                        tAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCa                <  1:5189043/76‑1 (MQ=255)

                                                        tAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCa                <  1:370983/76‑1 (MQ=255)

                                                        tAATCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGGTGATTCa                >  2:3062773/1‑76 (MQ=255)

                                                         aaTCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATcccg               >  1:6153058/1‑74 (MQ=255)

                                                         aaTCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCag               >  1:4834256/1‑76 (MQ=255)

                                                         aaTCCTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCag               <  2:1162735/76‑1 (MQ=255)

                                                             cTGGTCTGTATGACGTTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGGGGTGGTCCagaggg           >  1:2079765/1‑74 (MQ=255)

                                                             cTGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGGGGGGGTGATCTAGAgcg           >  1:3254152/1‑76 (MQ=255)

                                                              tGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGGGGCGTTGATCCCGAgggc          >  2:6625924/1‑76 (MQ=255)

                                                              tGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTTATCCCGAgcgg          >  1:2780235/1‑75 (MQ=255)

                                                              tGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAgcgc          <  2:39206/76‑1 (MQ=255)

                                                              tGGTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAgcgc          <  1:3818527/76‑1 (MQ=255)

                                                                gTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCgg        <  2:3685807/76‑1 (MQ=255)

                                                                gTCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCgg        <  2:3608814/76‑1 (MQ=255)

                                                                 tCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCGGa       <  2:5058733/76‑1 (MQ=255)

                                                                 tCTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCGGa       >  2:7708095/1‑76 (MQ=255)

                                                                 tCTGTATGACGGTGTGAAGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCCGa       >  2:2910800/1‑76 (MQ=255)

                                                                  cTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGGTCCAGAGCGCggca      >  2:5514590/1‑74 (MQ=255)

                                                                  cTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGGGCggga      >  1:894746/1‑74 (MQ=255)

                                                                  cTGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCGGaa      <  2:2496762/76‑1 (MQ=255)

                                                                   tGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCGGAAg     <  1:3786498/76‑1 (MQ=255)

                                                                   tGTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCGGAAg     <  2:3386396/76‑1 (MQ=255)

                                                                    gTATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCGGAAGt    <  2:7241898/76‑1 (MQ=255)

                                                                     tATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGGTCCCGAGCGCGGAAGtt   >  1:6229159/1‑76 (MQ=255)

                                                                     tATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCGGAAGtt   <  1:7269395/76‑1 (MQ=255)

                                                                     tATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCGGAAGtt   <  1:3014762/76‑1 (MQ=255)

                                                                     tATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCGGAAGtt   <  2:2354553/76‑1 (MQ=255)

                                                                     tATGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCGGAAGtt   <  1:6418956/76‑1 (MQ=255)

                                                                      aTGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCGGAAGTTa  <  2:5918981/76‑1 (MQ=255)

                                                                      aTGACGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCGGAAGTTa  <  2:2843655/76‑1 (MQ=255)

TACGCCTCCTTATTACAGGCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGGCGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCGGAAGTTA  >  REL606/3015051‑3015196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`