Predicted mutation
evidence seq id position mutation annotation gene description
RA REL606 3,251,818 C→T A315T (GCG→ACG)  nusA ← transcription elongation factor NusA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6063,251,8180CT100.0% 823.0 / NA 203A315T (GCG→ACG) nusAtranscription elongation factor NusA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (65/138);  total (65/138)

Only 100 of 207 total aligned reads displayed.

ATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTG  >  REL606/3251745‑3251889
                                                                         |                                                                       
 tcccGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAg                                                                      <  1:7449867/73‑1 (MQ=255)
 tcccGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAg                                                                      <  1:1397848/73‑1 (MQ=255)
  caggTTGAGTTCCAAACCGCTAAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAgg                                                                     <  2:1187911/73‑1 (MQ=255)
  caCGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAgg                                                                     >  1:2382719/1‑76 (MQ=255)
  caCGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAgg                                                                     <  2:5885560/76‑1 (MQ=255)
   aCGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGt                                                                    <  1:7141657/76‑1 (MQ=255)
    ggTTGAGTTCCCAACCGCTCAGCGGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGtt                                                                   <  1:6236046/75‑1 (MQ=255)
    cGTTGAGTTCCCAACCGCTCAGCTGCGAATCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGtt                                                                   <  2:5601438/76‑1 (MQ=255)
    cGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGtt                                                                   >  2:5284608/1‑76 (MQ=255)
    cGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGtt                                                                   >  2:2764931/1‑76 (MQ=255)
     tttGATTTCCCAACCGCTCAGCTGCAAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTa                                                                  <  2:6880220/75‑1 (MQ=255)
     tttGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTa                                                                  <  1:4223136/75‑1 (MQ=255)
     gTTGAGTTCCCACCCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTa                                                                  <  1:4556361/76‑1 (MQ=255)
     gTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTa                                                                  <  1:5579500/76‑1 (MQ=255)
     gTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTa                                                                  >  1:3041575/1‑76 (MQ=255)
        gAGTTCCCACCCGCTCGGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCg                                                               <  1:208604/76‑1 (MQ=255)
        gAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCg                                                               >  2:1607664/1‑76 (MQ=255)
         aGTTCCCACCCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCgg                                                              <  2:4675135/76‑1 (MQ=255)
          gTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGc                                                             >  2:646426/1‑76 (MQ=255)
            tCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCtt                                                           <  1:5688830/76‑1 (MQ=255)
             cccAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGGTACCGGGttt                                                          >  1:2635520/1‑75 (MQ=255)
              ccAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCa                                                         <  1:4293926/76‑1 (MQ=255)
               cacccGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCaa                                                        <  2:5069687/73‑1 (MQ=255)
               cacccGCTCAGCTGCAAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCaa                                                        <  1:6116119/73‑1 (MQ=255)
               cacccGCTCACCGGCGAAACCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCaa                                                        <  1:6172050/73‑1 (MQ=255)
               cAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCaa                                                        <  1:5808526/76‑1 (MQ=255)
               cAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCaa                                                        >  1:2593092/1‑76 (MQ=255)
                acccGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAAc                                                       <  1:2164761/74‑1 (MQ=255)
                aaCCGCTCGGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAAc                                                       <  2:624203/76‑1 (MQ=255)
                aaCCGCTAAGCTGCAAGGCCAGACGCCCTTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAAc                                                       <  2:7343493/76‑1 (MQ=255)
                    gcccAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCg                                                   <  1:589273/73‑1 (MQ=255)
                    gCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCCACGGCg                                                   >  1:7736473/1‑76 (MQ=255)
                    gCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCg                                                   <  1:4801051/76‑1 (MQ=255)
                    gCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCg                                                   <  2:2637802/76‑1 (MQ=255)
                    ccTCAGCGGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCg                                                   <  2:6842464/75‑1 (MQ=255)
                     cTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTTACCCGCGa                                                  >  2:6526299/1‑76 (MQ=255)
                      tCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTTCGTCAGGTTACCGGCTTCAACGGCGat                                                 <  1:4141599/76‑1 (MQ=255)
                      tCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGat                                                 <  2:7731229/76‑1 (MQ=255)
                       cAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGata                                                <  2:1542936/76‑1 (MQ=255)
                       cAGCTCCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGata                                                <  2:3524837/76‑1 (MQ=255)
                        aGCTCCGAAGCCAGACGCACGTTCTGCCCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGatat                                               <  1:3404219/76‑1 (MQ=255)
                         gCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATc                                              <  1:3648115/76‑1 (MQ=255)
                          cTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATcc                                             <  1:1471412/76‑1 (MQ=255)
                           tGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCa                                            <  1:2455790/76‑1 (MQ=255)
                           tGCGAACCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCa                                            <  2:41387/76‑1 (MQ=255)
                             cGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATg                                          <  2:2491950/76‑1 (MQ=255)
                              gAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTTCACGGCGGTATTCCTgg                                         >  1:4911281/1‑76 (MQ=255)
                               aacccAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGgt                                        <  2:6204668/73‑1 (MQ=255)
                               aaGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGgt                                        <  1:858750/76‑1 (MQ=255)
                               aaGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGACATCCATGgt                                        >  2:1796578/1‑76 (MQ=255)
                                aGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTTCACGGCGGTATCCATgggg                                       >  1:1292086/1‑74 (MQ=255)
                                  ccAGCCGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGgtgtg                                     <  2:2714770/76‑1 (MQ=255)
                                  ccAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTTCACGGCGGAATCCCTGgggtg                                     >  2:4785276/1‑76 (MQ=255)
                                   cAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTTCACGGCGATAATCATGgggtgt                                    >  2:2830255/1‑76 (MQ=255)
                                    aGACGCACGTTCTGGCCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGtt                                   <  2:3940261/76‑1 (MQ=255)
                                     gACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGttt                                  <  2:18826/76‑1 (MQ=255)
                                       cGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTAt                                <  2:930365/76‑1 (MQ=255)
                                       cGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTAt                                <  2:7222029/76‑1 (MQ=255)
                                         cACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCt                              <  1:1166192/76‑1 (MQ=255)
                                          aCGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTTAACGGCGATATCCCTGGTGTGTTTTTCtt                             >  2:3781775/1‑76 (MQ=255)
                                          aCGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCtt                             <  1:6552209/76‑1 (MQ=255)
                                             ttCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTTAAAGGCGATATTCATGGTGGGTTTttcttcct                          >  1:3148857/1‑74 (MQ=255)
                                               cTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTTAACGGCGGTATCCCTGGGGGGTTTTTCTTCAttc                        >  1:2519163/1‑74 (MQ=255)
                                               cTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCAGCTTCAACTGCGATATCCCTGGTGGGTTTATCTTCATcc                        >  1:2717228/1‑76 (MQ=255)
                                                tgtccGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATcca                       <  1:3511665/73‑1 (MQ=255)
                                                tGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATcca                       <  2:3701364/76‑1 (MQ=255)
                                                tGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATcca                       <  1:5284608/76‑1 (MQ=255)
                                                tGACCGATACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATcca                       <  2:2950508/76‑1 (MQ=255)
                                                 gACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTTCAAGGGGGTATCCATTGGGGGTTTTTTTTTATtcac                      >  2:4077617/1‑76 (MQ=255)
                                                 gACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGGTATCCATGGTGTGTTTATCTTCATccac                      >  1:5997769/1‑76 (MQ=255)
                                                 gACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATccac                      >  1:5470778/1‑76 (MQ=255)
                                                 gACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATccac                      <  1:3902694/76‑1 (MQ=255)
                                                   ccGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATccacca                    <  2:6566028/76‑1 (MQ=255)
                                                   ccGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATccacca                    >  2:7866787/1‑76 (MQ=255)
                                                   ccGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATccacca                    <  2:2120577/76‑1 (MQ=255)
                                                    ctttACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCcaccac                   <  2:2497840/74‑1 (MQ=255)
                                                    cGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCcaccac                   <  2:2070713/76‑1 (MQ=255)
                                                     gTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACg                  <  1:1978492/76‑1 (MQ=255)
                                                     gTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACg                  <  1:1215977/76‑1 (MQ=255)
                                                     gTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGGGTGTTTATCTTCATCCGCCACg                  >  1:7586717/1‑76 (MQ=255)
                                                        aCGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATa               <  2:1907761/76‑1 (MQ=255)
                                                          ggCCAATCGCCTGCGTCAGGTTACCGGCTTTAAAGGCGGTATCCATGGTGTGTTTATTTTTATCCACCACGGTAGa             >  2:7136974/1‑76 (MQ=255)
                                                          ggCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGa             <  2:3045268/76‑1 (MQ=255)
                                                           gCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTTATCCACCACGATAGaa            >  1:3989399/1‑76 (MQ=255)
                                                           gCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGaa            <  2:7673089/76‑1 (MQ=255)
                                                           gCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGaa            <  2:2906652/76‑1 (MQ=255)
                                                            ccAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACCATTGAAg           >  1:7225357/1‑76 (MQ=255)
                                                            ccAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGGTTATCTTTATTCAACACTATAGAAg           >  1:3543892/1‑76 (MQ=255)
                                                             cAATCGCCTGCGTCAGGTTACCGGCTTTAACGGCGGTATACCTGGGGTGTTTTTCTTCATCCACCCCGATAGAAga          >  2:2677627/1‑75 (MQ=255)
                                                              aaTCGCCTGCGTCAGGTTACCGGCTTTAACAGCGAAAACCATGGTGTGTTTTTTTTCATTCACCCCGATAGAAgga         >  2:6383956/1‑74 (MQ=255)
                                                              aaTCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCa         <  2:4947224/76‑1 (MQ=255)
                                                               aTCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCaa        <  1:87853/76‑1 (MQ=255)
                                                                  gCCTGCGTCAGGTTACCGGCTTCAACGGCGGTATACCTGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGt     >  2:7442756/1‑76 (MQ=255)
                                                                  gCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGt     >  1:7838571/1‑76 (MQ=255)
                                                                  gCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGt     <  1:4795597/76‑1 (MQ=255)
                                                                   ccTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTc    >  1:2444363/1‑76 (MQ=255)
                                                                    cTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCt   <  1:2899741/76‑1 (MQ=255)
                                                                    cTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCt   <  1:3788278/76‑1 (MQ=255)
                                                                    cTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCt   <  2:4351145/76‑1 (MQ=255)
                                                                     tGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTg  >  1:5094704/1‑76 (MQ=255)
                                                                         |                                                                       
ATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTG  >  REL606/3251745‑3251889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑