| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,328,651 | C→T | V110I (GTT→ATT) | mreB ← | cell wall structural complex MreBCD, actin‑like component MreB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,328,651 | 0 | C | T | 98.7% | 949.5 / 1.4 | 234 | V110I (GTT→ATT) | mreB | cell wall structural complex MreBCD, actin‑like component MreB |
| Reads supporting (aligned to +/- strand): ref base C (2/1); new base T (119/112); total (121/113) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.36e-01 | |||||||||||
Only 100 of 245 total aligned reads displayed.
CCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCAC > REL606/3328578‑3328723 | ccAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAAtgc > 2:5027332/1‑76 (MQ=255)ccAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAAtgc > 2:6852410/1‑76 (MQ=255)ccAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAAtgc > 2:3891762/1‑76 (MQ=255) aGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAAtgcgc > 1:4035717/1‑76 (MQ=255) aGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAAtgcgc > 2:2761188/1‑76 (MQ=255) gcgcCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAAtgcgcg < 2:7307086/76‑1 (MQ=255) cgcCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCgg < 1:1383937/76‑1 (MQ=255) gcCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCggg > 2:4401214/1‑76 (MQ=255) cccTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGc > 1:3335627/1‑76 (MQ=255) ccTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCt < 2:732880/76‑1 (MQ=255) ccTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCt > 1:5323373/1‑76 (MQ=255) gcgcGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTgg < 1:5752415/76‑1 (MQ=255) cgcgGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGa > 2:1422963/1‑76 (MQ=255) cgcgGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGa > 1:805012/1‑76 (MQ=255) cgcgGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGa < 1:3187723/76‑1 (MQ=255) cgGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACg > 2:7089978/1‑76 (MQ=255) cgGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACg > 2:7727160/1‑76 (MQ=255) cgGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACg < 2:802740/76‑1 (MQ=255) cgGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACg < 1:40801/76‑1 (MQ=255) ggATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGc > 1:879106/1‑76 (MQ=255) ggATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGc > 2:6901493/1‑76 (MQ=255) gATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCa > 1:7224071/1‑76 (MQ=255) gATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCa > 2:3823857/1‑76 (MQ=255) aTTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCAt < 1:7918176/76‑1 (MQ=255) aTTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCAt > 1:6578439/1‑76 (MQ=255) aTTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCAt > 1:376585/1‑76 (MQ=255) ttCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGAATa > 2:1638499/1‑76 (MQ=255) tCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATaa < 1:3937942/76‑1 (MQ=255) cACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATaaa > 1:1994531/1‑76 (MQ=255) cACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATaaa < 1:1973471/76‑1 (MQ=255) cACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATaaa < 1:2981416/76‑1 (MQ=255) cACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATaaa < 1:681139/76‑1 (MQ=255) cACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATaaa < 2:5340818/76‑1 (MQ=255) aCGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAg > 2:1374126/1‑76 (MQ=255) aaTTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTg > 1:5810986/1‑76 (MQ=255) aTTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGt > 2:3576127/1‑76 (MQ=255) tGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTg < 2:461746/76‑1 (MQ=255) cggcgTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCtgt > 1:6349145/1‑76 (MQ=255) cggcgTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCtgt > 2:3603767/1‑76 (MQ=255) ggcgTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCtgtg < 2:5883666/76‑1 (MQ=255) cgTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCa < 1:260344/76‑1 (MQ=255) gTTCAACCTGGTTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCAc < 1:1069840/76‑1 (MQ=255) gTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCAc > 1:4958174/1‑76 (MQ=255) ttCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACt > 1:7654324/1‑76 (MQ=255) ttCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACt > 1:4359714/1‑76 (MQ=255) aaCCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGt > 2:6331861/1‑76 (MQ=255) aaCCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGt > 2:2946576/1‑76 (MQ=255) aaCCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGt > 2:4540675/1‑76 (MQ=255) ccTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGttt < 1:646989/76‑1 (MQ=255) ccTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGttt < 2:219437/76‑1 (MQ=255) cTGGGTCACGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTtg < 1:4281955/76‑1 (MQ=255) tGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTtga > 1:2764882/1‑76 (MQ=255) tGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTtga < 1:1580486/76‑1 (MQ=255) tGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTtga > 2:1854335/1‑76 (MQ=255) tGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTtga < 1:757508/76‑1 (MQ=255) ggTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTtgatg > 2:4899956/1‑76 (MQ=255) ggTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTtgatg > 2:3501410/1‑76 (MQ=255) gTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTtgatga < 1:526311/76‑1 (MQ=255) gTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTtgatga > 2:6789687/1‑76 (MQ=255) cgcgCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAg < 1:4389987/76‑1 (MQ=255) cgcgCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAg < 2:307522/76‑1 (MQ=255) cgcgCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAg > 2:2315283/1‑76 (MQ=255) cgcCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTg > 2:7600227/1‑76 (MQ=255) cgcCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTg < 2:5763037/76‑1 (MQ=255) cgcCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTg < 2:7874525/76‑1 (MQ=255) ccAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCt > 1:128559/1‑76 (MQ=255) cAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTg < 2:5651880/76‑1 (MQ=255) cAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTg < 2:2071653/76‑1 (MQ=255) aaCCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTgg < 2:5117052/76‑1 (MQ=255) aCCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGa > 1:7431533/1‑76 (MQ=255) aCCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGa < 1:6275066/76‑1 (MQ=255) aCCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGa < 1:5897271/76‑1 (MQ=255) aCCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGa < 1:464522/76‑1 (MQ=255) aCCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGa < 1:5116589/76‑1 (MQ=255) aCCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGa < 2:313244/76‑1 (MQ=255) ccGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAg < 2:7466503/76‑1 (MQ=255) ccGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAg < 1:2875543/76‑1 (MQ=255) ccGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAg < 2:5503885/76‑1 (MQ=255) cGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGc > 2:3982333/1‑76 (MQ=255) cGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGc < 1:2851080/76‑1 (MQ=255) cGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGc > 2:5602843/1‑76 (MQ=255) ggCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCa < 1:7153396/76‑1 (MQ=255) gCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCAt < 2:1185419/76‑1 (MQ=255) gCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCAt > 2:2664886/1‑76 (MQ=255) cacaAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCAtttt > 2:4708550/1‑76 (MQ=255) acaAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCAttttt > 1:1089611/1‑76 (MQ=255) caAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCAtttttt < 1:4201426/76‑1 (MQ=255) aaaCCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTc > 2:6228160/1‑76 (MQ=255) aaaCCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTc > 2:2609260/1‑76 (MQ=255) aaaCCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTc < 1:6221182/76‑1 (MQ=255) aaCCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCa < 1:348322/76‑1 (MQ=255) aCCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAg > 2:6117101/1‑76 (MQ=255) aCCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAg < 2:7438202/76‑1 (MQ=255) ccAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGt > 1:609245/1‑76 (MQ=255) cAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTc < 1:7400827/76‑1 (MQ=255) aGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCa > 1:7794609/1‑76 (MQ=255) aGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCa > 1:5448330/1‑76 (MQ=255) aGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCa > 2:5624623/1‑76 (MQ=255) gAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCAc > 2:1174698/1‑76 (MQ=255) gAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCAc < 2:495301/76‑1 (MQ=255) | CCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCAC > REL606/3328578‑3328723 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |