BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,163,752	T→G	I966S (ATC→AGC)	rpoB →	DNA‑directed RNA polymerase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,163,752	0	T	G	100.0%	512.3 / NA	117	I966S (ATC→AGC)	rpoB	DNA‑directed RNA polymerase subunit beta
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (89/28); total (89/28)

Only 100 of 141 total aligned reads displayed.

ACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGA  >  REL606/4163680‑4163824

aCAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCt                                                                       >  2:5400043/1‑76 (MQ=255)

aCAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCt                                                                       >  1:1719396/1‑76 (MQ=255)

 cAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTc                                                                      >  1:2531721/1‑76 (MQ=255)

 cAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTc                                                                      <  2:6681600/76‑1 (MQ=255)

  aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCg                                                                     >  2:3322383/1‑76 (MQ=255)

  aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCg                                                                     <  1:6932053/76‑1 (MQ=255)

  aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCg                                                                     <  1:1496641/76‑1 (MQ=255)

    aCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGaa                                                                   <  2:888476/76‑1 (MQ=255)

    aCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGaa                                                                   <  2:366629/76‑1 (MQ=255)

    aCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGaa                                                                   <  1:3270997/76‑1 (MQ=255)

    aCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGaa                                                                   >  2:7665654/1‑76 (MQ=255)

     cGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAg                                                                  <  1:7976156/76‑1 (MQ=255)

     cGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAg                                                                  <  2:5760303/76‑1 (MQ=255)

       tGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCg                                                                >  2:7766416/1‑76 (MQ=255)

        gcgcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCgg                                                               <  2:149319/76‑1 (MQ=255)

        gcgcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCgg                                                               >  1:4885131/1‑76 (MQ=255)

         cgcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCggg                                                              >  1:237940/1‑76 (MQ=255)

         cgcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCggg                                                              >  2:850705/1‑76 (MQ=255)

          gcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGt                                                             <  1:6350401/76‑1 (MQ=255)

          gcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCCGGt                                                             >  2:1251021/1‑76 (MQ=255)

           cTGGAAATCGAAGAAATGCAGGTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTc                                                            >  1:1999142/1‑76 (MQ=255)

           cTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTc                                                            >  1:4387586/1‑76 (MQ=255)

           cTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTc                                                            <  2:3119238/76‑1 (MQ=255)

            tGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCt                                                           >  2:664517/1‑76 (MQ=255)

            tGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCt                                                           >  2:5391031/1‑76 (MQ=255)

             ggAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTg                                                          >  1:2785212/1‑76 (MQ=255)

             ggAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTg                                                          >  2:2951120/1‑76 (MQ=255)

             ggAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTg                                                          <  2:2072736/76‑1 (MQ=255)

              gAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGt                                                         >  1:1120582/1‑76 (MQ=255)

               aaaTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGtt                                                        <  1:6524077/76‑1 (MQ=255)

                 aTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCa                                                      <  2:7136944/76‑1 (MQ=255)

                 aTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCa                                                      <  1:6697803/76‑1 (MQ=255)

                 aTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCa                                                      <  2:5778215/76‑1 (MQ=255)

                  tcaaagaaATGCAGCACAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAg                                                     <  1:2413805/73‑1 (MQ=255)

                   cGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGc                                                    >  1:4622590/1‑76 (MQ=255)

                   cGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGc                                                    <  2:7616176/76‑1 (MQ=255)

                   cGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGc                                                    >  1:5686002/1‑76 (MQ=255)

                    gaagaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGcc                                                   >  2:5646539/1‑76 (MQ=255)

                    gaagaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGcc                                                   >  2:1880017/1‑76 (MQ=255)

                     aagaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCg                                                  >  1:4631953/1‑76 (MQ=255)

                     aagaaAGGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACGGCAGAGCCTCGAAGCGGGTCTGTTCAGCCg                                                  <  2:3334560/76‑1 (MQ=255)

                      agaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGt                                                 >  2:1899199/1‑76 (MQ=255)

                       aaaaTGCAGCTCAAACAGGCAAAGAAAGCCCTGTCTGAAGACCGGCAGACCCTCGAAGCGGGTCTGTTCAGCCGTa                                                <  2:5034134/75‑1 (MQ=255)

                         aaTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATc                                              <  1:1239926/76‑1 (MQ=255)

                           tGCAGCAAAAACAGGCGAAGAAAGCCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTTTTCAGCCGTATCCg                                            <  2:398990/76‑1 (MQ=255)

                             cAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtg                                          >  1:2091276/1‑76 (MQ=255)

                              aGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgc                                         >  1:3281323/1‑76 (MQ=255)

                                cTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCAgtgctg                                       >  2:2375576/1‑76 (MQ=255)

                                 tCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgt                                      >  2:3618846/1‑76 (MQ=255)

                                  cAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtg                                     >  1:7233225/1‑76 (MQ=255)

                                  cAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtg                                     >  1:2046688/1‑76 (MQ=255)

                                  cAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtg                                     <  1:5361712/76‑1 (MQ=255)

                                   aaaCAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtgc                                    >  1:3734023/1‑76 (MQ=255)

                                   aaaCAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtgc                                    >  1:4486922/1‑76 (MQ=255)

                                    aaCAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtgct                                   >  1:14788/1‑76 (MQ=255)

                                      cAGGCGAAAAAAGGCCTGTCTGAAGACCTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTgg                                 <  1:1373280/76‑1 (MQ=255)

                                      cAGGCGAAAAAAGACCTTTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTgg                                 <  1:911642/76‑1 (MQ=255)

                                       gggCGAATAAAACCCTGTCTGAAGCACTGCACAGCTTCGAGGCGGGTCTTTCCAGCCGTATCCGTGCTGTGCTGGt                                <  2:3581774/75‑1 (MQ=255)

                                       aGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGt                                >  1:1331414/1‑76 (MQ=255)

                                       aGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGt                                >  1:1484136/1‑76 (MQ=255)

                                       aGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGt                                >  2:6549557/1‑76 (MQ=255)

                                       aGGCGAAAAAAGCCTTGTCTGAAGACCGGCAGAGCCCGGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGt                                <  2:4938911/76‑1 (MQ=255)

                                         gcaaagaaggaCCTGTGTGAAGAGCGGGAGAGCCGCGAGGCGGGTTGGTTCAGCCGTATCCGTGCTGTGCTGGTAg                              <  2:2285131/73‑1 (MQ=255)

                                         gCGAAGAAAGCCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAg                              <  1:4569/76‑1 (MQ=255)

                                         gCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAg                              >  2:3787507/1‑76 (MQ=255)

                                            aagaaagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCg                           >  2:3347365/1‑76 (MQ=255)

                                            aagaaagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCg                           >  2:5625094/1‑76 (MQ=255)

                                            aaaaaagcCCTGTCTGAAGACCGGCAGAGCCTCGAGGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCg                           <  1:4707857/73‑1 (MQ=255)

                                             agaaagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCgg                          >  1:6131197/1‑76 (MQ=255)

                                              gaaagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCAGt                         >  2:4404371/1‑76 (MQ=255)

                                              gaaagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGGCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGt                         >  1:1185013/1‑76 (MQ=255)

                                               aaagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTg                        >  2:784358/1‑76 (MQ=255)

                                               aaagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTg                        >  2:5051703/1‑76 (MQ=255)

                                               aaagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTg                        >  2:587528/1‑76 (MQ=255)

                                               aaagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTg                        >  1:2131696/1‑76 (MQ=255)

                                                aagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTgg                       >  1:7234307/1‑76 (MQ=255)

                                                 agcccTGTTGGAAAAACGGCAGAGCCGCGAAGGGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGc                      <  1:4502240/73‑1 (MQ=255)

                                                  gaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCg                     >  2:6498749/1‑76 (MQ=255)

                                                    ccTGTTGGAAGAACTGCAGACCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGtt                   <  1:7002635/76‑1 (MQ=255)

                                                     cTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTg                  >  1:5419704/1‑76 (MQ=255)

                                                       gTCTGAAGACCGGCAGACCCTCAAGGCGGGTCTTTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGaa                <  1:2606672/76‑1 (MQ=255)

                                                       gTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTgca                >  1:5369376/1‑74 (MQ=255)

                                                       gTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGaa                >  1:4699798/1‑76 (MQ=255)

                                                        tCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAg               >  2:6999279/1‑76 (MQ=255)

                                                         cTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGc              >  2:3886313/1‑76 (MQ=255)

                                                         cTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGc              >  1:7486158/1‑76 (MQ=255)

                                                           gaagaaCTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTg            >  1:750788/1‑76 (MQ=255)

                                                            aagaaCTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTga           >  2:1435775/1‑76 (MQ=255)

                                                             aaaaCTCCAGGGCCCCGAGGGGGGTCTTTCCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTgag          <  1:6198801/74‑1 (MQ=255)

                                                              gaaCTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTgaga         >  1:4137355/1‑76 (MQ=255)

                                                              gaaCTGCAGAGCCTCGAAGCGGGTCTGTCCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTgaga         <  1:6159753/76‑1 (MQ=255)

                                                               aaCGGCAGAGCCGCGAAGGGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGaa        <  1:1880017/76‑1 (MQ=255)

                                                                aCTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGGTGTGCTGGTAGCCGGTGGCGTTGAAGGTGAGaat       >  2:7108891/1‑75 (MQ=255)

                                                                 cTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGc      >  1:2065310/1‑76 (MQ=255)

                                                                 cTGCAGACCCGGGAAGCGGTTCTGTCCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGc      <  1:717608/76‑1 (MQ=255)

                                                                  tGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCt     >  1:2924439/1‑76 (MQ=255)

                                                                  tGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCt     >  1:5414828/1‑76 (MQ=255)

                                                                   gCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGGAGCTGAGAAGCTc    >  1:301229/1‑76 (MQ=255)

                                                                    cAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCg   >  2:7599690/1‑76 (MQ=255)

                                                                     agagCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGa  >  1:6793319/1‑76 (MQ=255)

ACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGA  >  REL606/4163680‑4163824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`