| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,596,722 | C→T | A71A (GCG→GCA) | rsmC ← | 16S ribosomal RNA m2G1207 methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,596,722 | 0 | C | T | 99.6% | 944.6 / NA | 235 | A71A (GCG→GCA) | rsmC | 16S ribosomal RNA m2G1207 methyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (144/90); minor base G (0/1); total (144/91) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 268 total aligned reads displayed.
CATCAACTGGAACTGGGCTTCCGGTTTGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCCGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTGGCGGCTTAATACCTGCCAGTGGTGGAATTGC > REL606/4596647‑4596794 | tcaACTGGAACTGGGCTTCCGGTTTGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATctgc > 2:7323961/1‑76 (MQ=255) tcaACTGGAACTGGGCTTCCGGTTTGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATctgc > 2:4487567/1‑76 (MQ=255) tcaACTGGAACTGGGCTTCCGGTTTGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATctgc > 1:2950437/1‑76 (MQ=255) caACTGGAACTGGGCTTCCGGTTTGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATctgcc > 1:385214/1‑76 (MQ=255) caACTGGAACTGGGCTTCCGGTTTGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATctgcc > 2:3333530/1‑76 (MQ=255) caACTGGAACTGGGCTTCCGGTTTGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATctgcc > 1:89335/1‑76 (MQ=255) caACTGGAACTGGGCTTCCGGTTTGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATctgcc > 2:5572603/1‑76 (MQ=255) actggaactggGCTTCCGGTTTGTTCTTCGGCCAGTAGTAAATCAGTGTATCGCAATCTGCGACGTCATCTGCCGt < 2:3302455/76‑1 (MQ=255) actggaactggGCTTCCGGTTTGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGt < 1:617490/76‑1 (MQ=255) ctggaactggGCTTCCGGTTTGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTg > 2:1381500/1‑76 (MQ=255) tggacctggCGTTCCGGTTTTTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTgg < 1:6025761/76‑1 (MQ=255) tggaactggGCTTCCGGTTTGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTgg > 2:1941496/1‑76 (MQ=255) tggaactggGCTTCCGGTTTGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTgg < 2:4201396/76‑1 (MQ=255) ggaactggGCTTCCGGTTTGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGc > 2:4963133/1‑76 (MQ=255) ggaactggGCTTCCGGTTTGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGc > 1:4464724/1‑76 (MQ=255) gaactggGCTTCCGGTTTGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCg > 2:6248739/1‑76 (MQ=255) gaactggGCTTCCGGTTTGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCg < 2:6269138/76‑1 (MQ=255) actggGCTTCCGGTTTGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGAc > 1:304131/1‑76 (MQ=255) tggGCTTCCGGTTTGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCa > 2:2651078/1‑76 (MQ=255) tggGCTTCCGGTTTGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCa > 2:4560191/1‑76 (MQ=255) tggGCTTCCGGTTTGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCa < 1:4090614/76‑1 (MQ=255) tggGCTTCCGGTTTGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCa > 1:7050431/1‑76 (MQ=255) gggCTTCCGGTTTGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAg < 1:7514496/76‑1 (MQ=255) gCTTCCGGTTTGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGAc > 2:4266421/1‑76 (MQ=255) ttCCGGTTTGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTg > 1:7436614/1‑76 (MQ=255) ttCCGGTTTGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTg < 1:1015653/76‑1 (MQ=255) ttCCGGTTTGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTg < 1:5987364/76‑1 (MQ=255) ttCCGGTTTGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTg < 1:2767056/76‑1 (MQ=255) ttCCGGTTTGTGCTGCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTg < 2:3836590/76‑1 (MQ=255) ccGGTTTGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGaa > 1:4883959/1‑76 (MQ=255) ccGGTTTGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGaa < 1:7689432/76‑1 (MQ=255) ccGGTTTGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGaa > 1:5873825/1‑76 (MQ=255) cGGTTTGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGaaa < 1:6625079/76‑1 (MQ=255) cGGTTTGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGaaa > 1:255094/1‑76 (MQ=255) cGGTTTGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGaaa < 2:5339170/76‑1 (MQ=255) cGGTTTGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGaaa < 1:3553199/76‑1 (MQ=255) ggTTTGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAAc > 1:4686090/1‑76 (MQ=255) ggTTTGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAAc > 1:1899003/1‑76 (MQ=255) gTTTGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACg > 2:6836514/1‑76 (MQ=255) tttGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACgg > 1:5785782/1‑76 (MQ=255) tttGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACgg > 2:2416407/1‑76 (MQ=255) gTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCg > 1:4813639/1‑76 (MQ=255) gTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCg < 1:6437746/76‑1 (MQ=255) gTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCg < 1:4035920/76‑1 (MQ=255) ttcttcGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGt > 1:3101350/1‑76 (MQ=255) cttcGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTa > 2:6659346/1‑76 (MQ=255) tcGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATc > 2:2049084/1‑76 (MQ=255) gCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATcccc > 2:3997253/1‑76 (MQ=255) ccAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATccccc < 2:4369757/76‑1 (MQ=255) cAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCa > 1:6873088/1‑76 (MQ=255) agtagtAAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCAt > 1:240871/1‑76 (MQ=255) aggagtaAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCAt < 2:5952118/73‑1 (MQ=255) gtagtaAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATc > 1:3366229/1‑76 (MQ=255) tagtaAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCt < 1:1215230/76‑1 (MQ=255) tagtaAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCt < 1:7156128/76‑1 (MQ=255) agtaAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTg < 2:3752045/76‑1 (MQ=255) taAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGTGACCAGACTGAAACGGGCGTTATCCCCCATCTggc < 1:4278905/76‑1 (MQ=255) taAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATccccc > 1:2516940/1‑69 (MQ=255) taAATCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTggc > 1:4949685/1‑76 (MQ=255) aaaTCAGCGTATCGCAATCTGCGACGTCATCTGCCGTTGCGACCAGACTGAAACGGGCGTTATCCCCCATCTggcg > 2:5866293/1‑76 (MQ=255) aaaTCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTggcg > 1:2066984/1‑76 (MQ=255) aaTCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTggcgg > 1:5256575/1‑76 (MQ=255) aaTCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTggcgg > 1:2737324/1‑76 (MQ=255) aaTCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTggcgg > 2:314624/1‑76 (MQ=255) aaTCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTggcgg < 2:7748240/76‑1 (MQ=255) tCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTGGCGGCt < 2:1917625/76‑1 (MQ=255) tCAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTGGCGGCt > 2:5692111/1‑76 (MQ=255) cAGCGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTTGCGGCtt > 2:2536556/1‑76 (MQ=255) gCGTATCGAAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTGGCGGCTTaa < 1:1499203/76‑1 (MQ=255) cGTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTGGCGGCTTAAt > 2:7069423/1‑76 (MQ=255) gTATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTGGCGGCTTAATa > 1:823441/1‑76 (MQ=255) tATCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTGGCGGCTTAATAc > 1:5129287/1‑76 (MQ=255) aTCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTGGCGGCTTAATAcc > 2:672017/1‑76 (MQ=255) aTCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTGGCGGCTTAATAcc > 1:1611104/1‑76 (MQ=255) aTCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTGGCGGCTTAATAcc > 2:1722124/1‑76 (MQ=255) aTCGCAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTGGCGGCTTAATAcc > 1:3781332/1‑76 (MQ=255) tcccAACCTGCGCCTTCATCTGCCTGTTCGACCAGTCTGAAACGGGCGTTATCCCCCATCTGGCGGCTTAATACCt < 2:1527109/73‑1 (MQ=255) tCGCAATCGGCAACGTCATCTGGCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTGGCGGCTTAATAccc < 1:1289473/76‑2 (MQ=255) tCGCAAGCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTGGCGGCTTAATACCt < 1:7895391/76‑1 (MQ=255) cAATCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTGGCGGCTTAATACCTGcc > 1:1086457/1‑76 (MQ=255) aaTCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTGGCGGCTTAATACCTGCCa > 1:1268863/1‑76 (MQ=255) aaTCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTGGCGGCTTAATACCTGCCa < 1:5626052/76‑1 (MQ=255) aTCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTGGCGGCTTAATACCTGCCAg < 2:6372679/76‑1 (MQ=255) tCTGCGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTGGCGGCTTAATACCTGCCAgt > 2:6964677/1‑76 (MQ=255) cggcgcCGTCATCTGCCGTGGCGACCAGACTGAAACGGGCTTTATCCCCCATCGGGCGGCTTAACCCCTGCCAgtg < 2:828680/74‑1 (MQ=255) cTGCGCCGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTGGCGGCTTAATACCTGCCAgtg < 1:5579445/76‑1 (MQ=255) cGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTGGCGGCTTAATACCTGCCAgtggtg > 2:2375901/1‑76 (MQ=255) cGACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTGGCGGCTTAATACCTGCCAgtggtg > 1:4424113/1‑76 (MQ=255) gaggTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTGGCGGCTTAATACCTGCCAGtggtgg < 1:3684413/73‑1 (MQ=255) gACGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTGGCGGCTTAATACCTGCCAGtggtgg < 2:5660074/76‑1 (MQ=255) cGTCTTCGGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTGGCGGCTTAATACCTGCCAGTGGTGGaa < 1:5432034/76‑1 (MQ=255) cGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTGGCGGCTTAATACCTGCCAGTGGTGGaa < 1:6302793/76‑1 (MQ=255) cGTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTGGCGGCTTAATACCTGCCAGTGGTGGaa < 1:7864109/76‑1 (MQ=255) gTCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTGGCGGCTTAATACCTGCCAGTGGTGGAAt > 2:1103413/1‑76 (MQ=255) tCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTGGCGGCTTAATACCTGCCAGTGGTGGAAtt > 2:2952901/1‑76 (MQ=255) tCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTGGCGGCTTAATACCTGCCAGTGGTGGAAtt > 1:6366980/1‑76 (MQ=255) tCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTGGCGGCTTAATACCTGCCAGTGGTGGAAtt > 1:6500392/1‑76 (MQ=255) tCATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTGGCGGCTTAATACCTGCCAGTGGTGGAAtt < 1:6817269/76‑1 (MQ=255) cATCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTGGCGGCTTAATACCTGCCAGTGGTGGAATTg > 1:947317/1‑76 (MQ=255) aTCTGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTGGCGGCTTAATACCTGCCAGTGGTGGAATTGc > 2:2665583/1‑76 (MQ=255) | CATCAACTGGAACTGGGCTTCCGGTTTGTTCTTCGGCCAGTAGTAAATCAGCGTATCGCAATCTGCGACGTCATCCGCCGTGGCGACCAGACTGAAACGGGCGTTATCCCCCATCTGGCGGCTTAATACCTGCCAGTGGTGGAATTGC > REL606/4596647‑4596794 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |