BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,580,357	G→A	intergenic (‑63/+1)	yneH ← / ← yneI	predicted glutaminase/predicted aldehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,580,357	0	G	A	100.0%	990.1 / NA	239	intergenic (‑63/+1)	yneH/yneI	predicted glutaminase/predicted aldehyde dehydrogenase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (136/103); total (136/103)

Only 100 of 246 total aligned reads displayed.

GCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACC  >  REL606/1580284‑1580429

 cGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCa                                                                       <  2:3035639/76‑1 (MQ=255)

 cGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCa                                                                       <  2:2644430/76‑1 (MQ=255)

 cGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCa                                                                       >  2:6027288/1‑76 (MQ=255)

 cGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCa                                                                       >  1:6230578/1‑76 (MQ=255)

  gACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAg                                                                      >  2:6744204/1‑76 (MQ=255)

   aCTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGa                                                                     >  1:1708113/1‑76 (MQ=255)

    ctcccACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGAt                                                                    <  2:854574/73‑1 (MQ=255)

    cTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGAt                                                                    >  1:7834007/1‑76 (MQ=255)

    cTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGAt                                                                    <  1:5207093/76‑1 (MQ=255)

     tGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATc                                                                   >  2:5952508/1‑76 (MQ=255)

     tGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATc                                                                   >  2:7163910/1‑76 (MQ=255)

     tGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATc                                                                   >  1:2969033/1‑76 (MQ=255)

       ccACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCg                                                                 >  2:5347971/1‑76 (MQ=255)

         aCTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGt                                                               >  2:4914986/1‑76 (MQ=255)

         aCTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGt                                                               >  2:4113452/1‑76 (MQ=255)

         aCTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGt                                                               >  2:4794494/1‑76 (MQ=255)

         aCTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGt                                                               >  2:4605995/1‑76 (MQ=255)

           tttCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCt                                                             >  2:1300013/1‑76 (MQ=255)

           tttCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCt                                                             <  2:6652875/76‑1 (MQ=255)

            ttCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCtt                                                            >  1:2944492/1‑76 (MQ=255)

            ttCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCtt                                                            <  2:7699347/76‑1 (MQ=255)

            ttCTACTCCTGGACCGCAGGGCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCtt                                                            >  1:2615429/1‑76 (MQ=255)

             tCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCttt                                                           >  1:1483168/1‑76 (MQ=255)

              cTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTc                                                          >  1:1001641/1‑76 (MQ=255)

              cTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTc                                                          <  2:4045633/76‑1 (MQ=255)

              cTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTc                                                          >  1:2424994/1‑76 (MQ=255)

              cTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTc                                                          >  2:5664981/1‑76 (MQ=255)

               tACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTcc                                                         >  2:1485460/1‑76 (MQ=255)

                aCTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCca                                                        >  2:553154/1‑76 (MQ=255)

                 cTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCcac                                                       <  2:7570330/76‑1 (MQ=255)

                  tCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCcaca                                                      <  2:6230578/76‑1 (MQ=255)

                  tCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCcaca                                                      <  2:331037/76‑1 (MQ=255)

                   ccTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATCTGTCGCATCAGATCCGGTCTTTCCacac                                                     >  2:3614876/1‑76 (MQ=255)

                   ccTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCacac                                                     >  2:7263945/1‑76 (MQ=255)

                    cTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACAcc                                                    >  1:4388829/1‑76 (MQ=255)

                     tGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCg                                                   >  2:5225666/1‑76 (MQ=255)

                      ggACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGt                                                  <  2:6383643/76‑1 (MQ=255)

                        aCCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCt                                                <  1:7692635/76‑1 (MQ=255)

                        aCCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCt                                                <  2:2065180/76‑1 (MQ=255)

                        aCCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCt                                                >  2:1096006/1‑76 (MQ=255)

                          cGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTgg                                              <  1:4738414/76‑1 (MQ=255)

                            cAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGat                                            >  2:7355647/1‑76 (MQ=255)

                             aGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGata                                           <  2:2226875/76‑1 (MQ=255)

                                tCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTa                                        >  2:3686058/1‑76 (MQ=255)

                                tCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTa                                        >  1:5644843/1‑76 (MQ=255)

                                 cTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTAc                                       >  1:3235990/1‑76 (MQ=255)

                                 cTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTAc                                       <  1:7769107/76‑1 (MQ=255)

                                 cTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTAc                                       <  2:5277120/76‑1 (MQ=255)

                                  tGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACa                                      >  1:7316868/1‑76 (MQ=255)

                                  tGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACa                                      >  1:3413969/1‑76 (MQ=255)

                                      aaGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAAt                                  <  1:6158923/76‑1 (MQ=255)

                                      aaGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAAt                                  >  2:1276657/1‑76 (MQ=255)

                                       aGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAAtt                                 <  2:6811785/76‑1 (MQ=255)

                                       aGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAAtt                                 <  2:828333/76‑1 (MQ=255)

                                       aGACCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAAtt                                 <  1:6780586/76‑1 (MQ=255)

                                         aCCTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCg                               >  1:3849489/1‑76 (MQ=255)

                                          ccTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCgt                              >  2:1777616/1‑76 (MQ=255)

                                          ccTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCgt                              <  2:7510644/76‑1 (MQ=255)

                                           cTGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCgtg                             >  2:4522638/1‑76 (MQ=255)

                                            tGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCgtgt                            >  2:2141679/1‑76 (MQ=255)

                                            tGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCgtgt                            >  1:2154246/1‑76 (MQ=255)

                                            tGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCgtgt                            >  2:3283868/1‑76 (MQ=255)

                                            tGCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCgtgt                            >  1:2019240/1‑76 (MQ=255)

                                             gCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCGACACCGTCTGGATATTACAGAATTCGTGTa                           <  1:6744382/76‑1 (MQ=255)

                                             gCGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTa                           >  1:828804/1‑76 (MQ=255)

                                              cGAGTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTaa                          >  1:3660816/1‑76 (MQ=255)

                                                 gTATATCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGcc                       <  2:1980626/76‑1 (MQ=255)

                                                  tataTCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCg                      >  2:244821/1‑76 (MQ=255)

                                                   atatCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTaa                          <  2:3878885/71‑1 (MQ=255)

                                                   atatCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTaa                          <  2:2498253/71‑1 (MQ=255)

                                                   atatCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTaa                          >  1:5478877/1‑71 (MQ=255)

                                                   atatCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGa                     >  2:6984385/1‑76 (MQ=255)

                                                     atCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGaaa                   >  1:6628349/1‑76 (MQ=255)

                                                      tCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAAt                  <  2:3488369/76‑1 (MQ=255)

                                                      tCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAAt                  <  1:2087852/76‑1 (MQ=255)

                                                      tCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAAt                  <  1:5679291/76‑1 (MQ=255)

                                                      tCAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAAt                  <  1:7430029/76‑1 (MQ=255)

                                                       cAGAGCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATg                 <  1:4794494/76‑1 (MQ=255)

                                                        agagCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATgg                <  2:5083954/76‑1 (MQ=255)

                                                        agagCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATgg                >  2:3764561/1‑76 (MQ=255)

                                                         gagCTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATggg               <  2:3349798/76‑1 (MQ=255)

                                                            cTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGaaa            >  1:6817112/1‑76 (MQ=255)

                                                            cTGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGaaa            <  1:4166860/76‑1 (MQ=255)

                                                             tGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAg           >  2:3092076/1‑76 (MQ=255)

                                                             tGAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAg           >  2:1592731/1‑76 (MQ=255)

                                                              gAATATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGc          <  1:6305721/76‑1 (MQ=255)

                                                               aaTATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCt         <  1:1422807/76‑1 (MQ=255)

                                                               aaTATGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCATGTAAGCCGAAATGGGAAAGCt         <  2:6047945/76‑1 (MQ=255)

                                                                atatGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTc        >  2:1456163/1‑76 (MQ=255)

                                                                 tatGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCa       >  2:4837722/1‑76 (MQ=255)

                                                                 tatGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCa       <  1:1218813/76‑1 (MQ=255)

                                                                  atGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCAc      <  2:3448961/76‑1 (MQ=255)

                                                                  atGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCAc      >  1:1188855/1‑76 (MQ=255)

                                                                  atGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCAc      <  1:2357079/76‑1 (MQ=255)

                                                                  atGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCAc      >  1:5934651/1‑76 (MQ=255)

                                                                   tGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACg     >  2:7118396/1‑76 (MQ=255)

                                                                   tGTCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACg     >  2:6551242/1‑76 (MQ=255)

                                                                     tCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGAc   <  2:5957968/76‑1 (MQ=255)

                                                                     tCGCATCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGAc   >  1:7067842/1‑76 (MQ=255)

                                                                      cgcaTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGAcc  >  2:7784484/1‑76 (MQ=255)

GCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACC  >  REL606/1580284‑1580429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`