| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,697,996 | T→G | D8A (GAT→GCT) | slyA ← | transcriptional regulator SlyA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,697,996 | 0 | T | G | 98.4% | 1064.2 / 3.1 | 247 | D8A (GAT→GCT) | slyA | transcriptional regulator SlyA |
| Reads supporting (aligned to +/- strand): ref base T (3/1); new base G (100/143); total (103/144) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01 | |||||||||||
Only 100 of 255 total aligned reads displayed.
TGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAAT > REL606/1697921‑1698068 | gggTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATc < 1:1136937/76‑1 (MQ=255) ggTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCa < 2:7914698/76‑1 (MQ=255) ggTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCa > 2:7425903/1‑76 (MQ=255) ggTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCa < 1:5864271/76‑1 (MQ=255) ggTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCa < 1:5650450/76‑1 (MQ=255) ggTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCa > 1:2156185/1‑76 (MQ=255) gTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAg > 1:1380506/1‑76 (MQ=255) gTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAg > 2:4168097/1‑76 (MQ=255) gTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAg < 1:4099659/76‑1 (MQ=255) gTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAg > 2:3310003/1‑76 (MQ=255) tttGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGa < 1:7845179/76‑1 (MQ=255) tttGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGa > 2:6947820/1‑76 (MQ=255) tttGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGa < 1:4976289/76‑1 (MQ=255) ttGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGaa < 2:5977559/76‑1 (MQ=255) ttGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGaa < 2:4475054/76‑1 (MQ=255) gtgtTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAAcc > 2:6511886/1‑76 (MQ=255) gtgtTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAAcc > 1:3846587/1‑76 (MQ=255) tgtTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCt > 1:688144/1‑76 (MQ=255) tgtTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCt < 1:23185/76‑1 (MQ=255) gtTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTa < 2:1430869/76‑1 (MQ=255) gtTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTa < 1:1617647/76‑1 (MQ=255) tAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGt > 2:704849/1‑76 (MQ=255) tAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGt > 2:4940170/1‑76 (MQ=255) aaCTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTg < 2:7265887/76‑1 (MQ=255) aCTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTgg < 1:3406487/76‑1 (MQ=255) cTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGc < 1:6333632/76‑1 (MQ=255) tCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCg < 1:5937238/76‑1 (MQ=255) tCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCg > 2:3098504/1‑76 (MQ=255) ccAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGa > 2:6851169/1‑76 (MQ=255) ccAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGa > 2:5438913/1‑76 (MQ=255) cggCGGTTTCAGGCGATGGTCTATAAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGAt < 2:4493563/74‑1 (MQ=255) cAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGAt < 2:5025427/76‑1 (MQ=255) aGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGAtt > 2:5367711/1‑76 (MQ=255) gCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTc < 2:5861954/76‑1 (MQ=255) cGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTcc < 1:3535933/76‑1 (MQ=255) gTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCaa < 1:4253725/76‑1 (MQ=255) tttCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAAt > 1:1198982/1‑76 (MQ=255) ttCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCacat > 2:4782393/1‑73 (MQ=255) ttCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAAtt > 2:2528794/1‑76 (MQ=255) ttCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAAtt > 1:6512849/1‑76 (MQ=255) ttCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAAtt > 2:7320719/1‑76 (MQ=255) ttCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAAtt > 1:5524890/1‑76 (MQ=255) tCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAAttt < 2:719239/76‑1 (MQ=255) ggCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCAt < 1:6241513/76‑1 (MQ=255) ggCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCAt < 2:2293056/76‑1 (MQ=255) cGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCAtct < 1:6434211/76‑1 (MQ=255) cGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCAtct > 1:5027046/1‑76 (MQ=255) gATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCAtctc < 1:1059855/76‑1 (MQ=255) tGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCt > 2:7670333/1‑76 (MQ=255) ggTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCtt > 2:2332783/1‑76 (MQ=255) ggTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCtt < 1:1426675/76‑1 (MQ=255) ggTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCtt < 2:3576414/76‑1 (MQ=255) ggTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCtt > 2:5753997/1‑76 (MQ=255) gTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTta > 1:4370633/1‑76 (MQ=255) tCTATCAGAGCACGCCATATGCGCACCAACCGTTCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTtat < 1:5057065/76‑1 (MQ=255) tCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTtat > 2:560759/1‑76 (MQ=255) tCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTtat > 2:2918962/1‑76 (MQ=255) tATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATaa < 2:169166/76‑1 (MQ=255) tATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATaa > 2:4657637/1‑76 (MQ=255) tATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATaa < 1:3213810/76‑1 (MQ=255) aTCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAAt > 2:6144410/1‑76 (MQ=255) aTCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAAt > 2:1065177/1‑76 (MQ=255) tCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAAtt < 1:772262/76‑1 (MQ=255) cAGAGCTCGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTa < 2:5188923/76‑1 (MQ=255) cAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTa > 2:6339803/1‑76 (MQ=255) cAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTa < 1:6339475/76‑1 (MQ=255) cAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTa < 2:1627671/76‑1 (MQ=255) agagCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAg > 1:817295/1‑76 (MQ=255) agagCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAg > 1:1241440/1‑76 (MQ=255) gagCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGc < 2:3376341/76‑1 (MQ=255) gagCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGc > 2:6232677/1‑76 (MQ=255) gCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCtt < 2:1001695/76‑1 (MQ=255) cACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCCATTTCATCTCCTTATAATTAGCTTg > 2:1953654/1‑76 (MQ=255) cGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCt < 2:6484330/76‑1 (MQ=255) cGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCt > 2:5713715/1‑76 (MQ=255) gCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTa > 1:188439/1‑76 (MQ=255) gCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTa < 1:5815414/76‑1 (MQ=255) gCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTa < 2:1987567/76‑1 (MQ=255) ccATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCGATTTCATCTCCTTATAATTAGCTTGCTaa > 2:3801291/1‑76 (MQ=255) ccATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTaa < 1:6403505/76‑1 (MQ=255) cATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAg < 2:4343552/76‑1 (MQ=255) atatGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGa < 2:3640815/76‑1 (MQ=255) tatGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGat < 1:7087518/76‑1 (MQ=255) atGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGata < 2:2629233/76‑1 (MQ=255) atGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGata > 1:3055485/1‑76 (MQ=255) atGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGata < 2:6986994/76‑1 (MQ=255) atGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGata < 1:4732029/76‑1 (MQ=255) gcgcACCAACCGTTCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGAtatt < 1:522268/76‑1 (MQ=255) gcgcACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGAtatt < 1:4279860/76‑1 (MQ=255) cgcTCCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGAtatta < 1:5848980/76‑1 (MQ=255) cACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATg < 1:5293918/76‑1 (MQ=255) ccAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCg < 1:7320719/76‑1 (MQ=255) aaCCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGc > 1:4557774/1‑76 (MQ=255) ccGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCtt < 1:83365/76‑1 (MQ=255) ccGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCtt > 1:4942261/1‑76 (MQ=255) gTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCtttt > 2:3188651/1‑76 (MQ=255) ttccAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTa < 2:4197818/74‑1 (MQ=255) tGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTa < 1:256580/76‑1 (MQ=255) gCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAg > 1:7365138/1‑76 (MQ=255) agagCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAAt > 2:3913447/1‑76 (MQ=255) | TGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAAT > REL606/1697921‑1698068 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |