| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,193,779 | G→T | L143I (CTT→ATT) | yeiB ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,193,779 | 0 | G | T | 99.4% | 619.6 / NA | 165 | L143I (CTT→ATT) | yeiB | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (56/108); minor base C (0/1); total (56/109) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 187 total aligned reads displayed.
CGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA > REL606/2193708‑2193851 | cGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATcccc < 2:6558652/76‑1 (MQ=255) gATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGGC‑GGGAATTAACCCCCAAAACACCCAAACACCCAAccccc > 2:3533994/1‑75 (MQ=255) aTGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccac < 2:2283813/76‑1 (MQ=255) aTGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccac < 1:4453649/76‑1 (MQ=255) gCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTC‑GGGAAATAGCCCCCATAACAACAAAACGCCCAACCccaccc > 1:4775752/1‑75 (MQ=255) cATCCGGCGTCCAGGCGCGGCTGGGCTGGGGGGT‑GGAAATCAACCCCAACAACCACCAAAAGCCCACTCccaccca > 2:4433161/1‑74 (MQ=255) cATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGGC‑GGGAAACAGCCCCAATAACAACCAAAACCCAAACCccacccc > 2:7382585/1‑74 (MQ=255) tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGAt < 1:7102693/76‑1 (MQ=255) tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAACCCCCACCAGAt < 1:1007856/76‑1 (MQ=255) ccGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATa < 2:2965361/76‑1 (MQ=255) ccGGCGTCCAGGCGCGGCTGGGCTGGCTGTC‑GGAAATCAGCCCCCATAACAGCAAAACGCCAATCCCCACCAGATa > 1:7649120/1‑76 (MQ=255) cGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaa < 1:6131967/76‑1 (MQ=255) cGGCGTCCAGGCGCGGCTGGTCTGGCTGCC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaa < 1:6662708/76‑1 (MQ=255) cGGCGTCCAGGCGCGGCTGGGCTGGCTGTCGGGAAATAACCCCCAA‑AACAGCAAAACGCCAAACCCCAACACATaa > 2:3249353/1‑76 (MQ=255) ggCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaaa < 2:5872143/76‑1 (MQ=255) ggCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaaa < 1:240260/76‑1 (MQ=255) ggCGTCCAGGCGCGGCTGGGCTGGCTGTC‑GGGAAATAGGCCCCATAAAAGCAAAAACCCAAACCCCCCCACGTaaa > 2:1534805/1‑76 (MQ=255) gCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAg < 1:5115046/76‑1 (MQ=255) gCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAg < 2:1567480/76‑1 (MQ=255) gCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAg < 2:2067184/76‑1 (MQ=255) gTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCa < 1:7042229/76‑1 (MQ=255) tCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCAt < 1:868992/76‑1 (MQ=255) ccAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATg < 1:5317753/76‑1 (MQ=255) ccAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATg < 1:6625209/76‑1 (MQ=255) gcgcGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGc > 1:6278159/1‑76 (MQ=255) cgGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTg < 2:4546840/76‑1 (MQ=255) cgGCTGGTCTGGCTGTC‑GGAAAACAGCCCCAATAACAGCAAAACGCCAATACCCCCCAGATAAAGCATGACGcccg > 2:2134902/1‑74 (MQ=255) cTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt < 1:770651/76‑1 (MQ=255) cTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt > 1:5353535/1‑76 (MQ=255) cTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt < 1:5708509/76‑1 (MQ=255) tGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt > 2:7946101/1‑76 (MQ=255) tGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt < 1:3226502/76‑1 (MQ=255) tGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt < 1:4561083/76‑1 (MQ=255) ggTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa < 1:4356203/76‑1 (MQ=255) ggcTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa < 2:4233848/74‑1 (MQ=255) gTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa < 2:6222096/76‑1 (MQ=255) tCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa < 2:5493360/76‑1 (MQ=255) tCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa > 2:3089852/1‑76 (MQ=255) cTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc < 1:759411/76‑1 (MQ=255) tGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa < 2:2198109/76‑1 (MQ=255) gCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAgg < 1:2980768/76‑1 (MQ=255) cTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGc > 2:6441758/1‑76 (MQ=255) cTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGc > 2:5989466/1‑76 (MQ=255) tGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt < 1:6458218/76‑1 (MQ=255) tGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt > 1:608637/1‑76 (MQ=255) gTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt < 2:7164357/76‑1 (MQ=255) gTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt > 1:3336107/1‑76 (MQ=255) tC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt < 1:1485509/76‑1 (MQ=255) tC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt < 1:4403573/76‑1 (MQ=255) tC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt < 2:2863753/76‑1 (MQ=255) c‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt < 1:6756744/76‑1 (MQ=255) c‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt > 1:2511878/1‑76 (MQ=255) c‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt < 2:5951326/76‑1 (MQ=255) ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt < 1:4047937/76‑1 (MQ=255) ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt < 2:2868770/76‑1 (MQ=255) gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa < 1:6345861/76‑1 (MQ=255) gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa < 2:5122481/76‑1 (MQ=255) gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa < 2:6146368/76‑1 (MQ=255) aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc > 2:406086/1‑76 (MQ=255) aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc > 2:2671938/1‑76 (MQ=255) aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc > 1:6795326/1‑76 (MQ=255) aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc < 2:5242860/76‑1 (MQ=255) aTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg > 2:5809760/1‑76 (MQ=255) gCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATgg > 2:1424090/1‑76 (MQ=255) ccccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc < 1:7354702/76‑1 (MQ=255) cccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcg < 1:276421/76‑1 (MQ=255) cAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATTgcgct > 2:7075391/1‑75 (MQ=255) cAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCa > 1:7625728/1‑76 (MQ=255) cAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCa < 1:951421/76‑1 (MQ=255) cAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCa > 1:4203837/1‑76 (MQ=255) cAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCa < 2:128820/76‑1 (MQ=255) aaTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCAt < 1:5650572/76‑1 (MQ=255) aaTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCAt < 1:2066713/76‑1 (MQ=255) aTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc > 1:1128619/1‑76 (MQ=255) aTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc < 2:3287862/76‑1 (MQ=255) aTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc > 1:47258/1‑76 (MQ=255) tAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg < 2:6715445/76‑1 (MQ=255) aaCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc > 2:7555078/1‑76 (MQ=255) aCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgcg < 2:6938885/76‑1 (MQ=255) cAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAGCAGGCTTTTTACCGATGGCGCATCgcgc < 1:2557390/76‑1 (MQ=255) aGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCa > 2:1206596/1‑76 (MQ=255) gCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAc > 1:168491/1‑76 (MQ=255) cAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAcc > 2:1430738/1‑76 (MQ=255) cAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAcc > 1:5666018/1‑76 (MQ=255) aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa > 1:1922627/1‑76 (MQ=255) aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa < 2:3796466/76‑1 (MQ=255) aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa > 1:6488921/1‑76 (MQ=255) aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa < 2:6040670/76‑1 (MQ=255) aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa < 2:1891881/76‑1 (MQ=255) aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa < 2:3224497/76‑1 (MQ=255) aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg < 2:2536277/76‑1 (MQ=255) aCGCCAATCCCCGCCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgcc < 2:4606810/76‑1 (MQ=255) aCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgcc > 1:6858746/1‑76 (MQ=255) aCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgcc > 2:6458584/1‑76 (MQ=255) aCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgcc < 1:786933/76‑1 (MQ=255) aCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgcc < 2:6671958/76‑1 (MQ=255) cGCCAACCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccg < 1:5862526/76‑1 (MQ=255) gCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCcgccgc > 2:931512/1‑76 (MQ=255) ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc > 1:7495029/1‑76 (MQ=255) cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca > 1:4546221/1‑76 (MQ=255) | CGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA > REL606/2193708‑2193851 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |