| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,762,428 | C→T | intergenic (‑16/+47) | rpoS ← / ← nlpD | RNA polymerase sigma factor/predicted outer membrane lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,762,428 | 0 | C | T | 99.3% | 1241.3 / ‑2.6 | 295 | intergenic (‑16/+47) | rpoS/nlpD | RNA polymerase sigma factor/predicted outer membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base T (155/138); total (155/140) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01 | |||||||||||
Only 100 of 301 total aligned reads displayed.
TCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCG > REL606/2762355‑2762500 | tctcATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGt > 2:4507313/1‑76 (MQ=255) ctcATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCtgtg > 2:3008847/1‑76 (MQ=255) ctcATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCtgtg > 2:6657127/1‑76 (MQ=255) catcaAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGAt > 1:2067022/1‑76 (MQ=255) tcaAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATcc > 1:1332019/1‑76 (MQ=255) caAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATccc < 1:3603987/76‑1 (MQ=255) caAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATccc < 1:4561796/76‑1 (MQ=255) caAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATccc > 2:6747170/1‑76 (MQ=255) caAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATccc < 1:2469573/76‑1 (MQ=255) aaaTTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCt > 2:6952629/1‑76 (MQ=255) aaTTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCtt < 2:6036880/76‑1 (MQ=255) aTTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTg < 1:5870146/76‑1 (MQ=255) ttCCGCATCTTCATTTAAATCATGACCTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGa < 1:5125660/76‑1 (MQ=255) tCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGAc < 2:7241937/76‑1 (MQ=255) cGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACgg > 1:1176565/1‑76 (MQ=255) cGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACgg < 2:5651892/76‑1 (MQ=255) gCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGa > 2:7021288/1‑76 (MQ=255) cATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGaa > 1:7277117/1‑76 (MQ=255) cATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGaa > 2:7659723/1‑76 (MQ=255) aTCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAAc > 1:1010754/1‑76 (MQ=255) aTCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAAc > 2:3598612/1‑76 (MQ=255) aTCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAAc > 2:6571428/1‑76 (MQ=255) aTCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAAc > 2:2171523/1‑76 (MQ=255) tCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACa < 2:1084809/76‑1 (MQ=255) cTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACAt < 1:6445701/76‑1 (MQ=255) cTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACAt < 1:1924013/76‑1 (MQ=255) ttCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACAtt < 2:3269712/76‑1 (MQ=255) ttCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACAtt > 2:4483226/1‑76 (MQ=255) ttCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACAtt > 2:2870056/1‑76 (MQ=255) tCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTc > 2:1332444/1‑76 (MQ=255) cATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCa > 1:3765420/1‑76 (MQ=255) cATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCa < 2:434061/76‑1 (MQ=255) cATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCa < 1:5049654/76‑1 (MQ=255) aTTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCaa < 1:2645657/76‑1 (MQ=255) ttAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGc > 1:413204/1‑76 (MQ=255) ttAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGc > 2:6927975/1‑76 (MQ=255) tAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCa > 1:6746132/1‑76 (MQ=255) aaTCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCa < 1:6224400/70‑1 (MQ=255) aaTCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCa < 1:1372302/70‑1 (MQ=255) aaTCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCa > 2:1372302/1‑70 (MQ=255) aaTCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCaaa > 2:7116112/1‑76 (MQ=255) aaTCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCaaa < 2:2288422/76‑1 (MQ=255) aTCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCaaaa < 1:615007/76‑1 (MQ=255) aTCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCaaaa < 1:950363/76‑1 (MQ=255) aTCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCaaaa > 1:2316460/1‑76 (MQ=255) cATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGAAACATTCAAGCAAAAGc > 2:5909562/1‑76 (MQ=255) aTGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGcc < 2:7802253/76‑1 (MQ=255) gAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTg < 2:6794043/76‑1 (MQ=255) gAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTg < 1:3709480/76‑1 (MQ=255) gAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTg < 2:2024118/76‑1 (MQ=255) aCTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGt < 1:4202131/76‑1 (MQ=255) aCTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGt < 1:3844764/76‑1 (MQ=255) tttCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTc > 2:4069046/1‑76 (MQ=255) ttCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTcc > 2:6298290/1‑76 (MQ=255) ttCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTcc < 2:4948965/76‑1 (MQ=255) ttCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTcc > 1:1976550/1‑76 (MQ=255) tCAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTccg < 1:3944566/76‑1 (MQ=255) cAGCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATCCAAGCAAAAGCCTGGTTccgc < 1:5600489/76‑1 (MQ=255) gCGTATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTccgccg < 1:1103278/76‑1 (MQ=255) tATTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGAtt > 1:2846336/1‑76 (MQ=255) aTTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGAttt < 2:4236478/76‑1 (MQ=255) aTTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGAttt > 2:5352175/1‑76 (MQ=255) aTTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGAttt < 1:3080731/76‑1 (MQ=255) aTTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGAttt > 1:7388579/1‑76 (MQ=255) aTTCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGAttt > 1:6686389/1‑76 (MQ=255) ttCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTa < 1:487272/76‑1 (MQ=255) tCTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTAt > 1:5390207/1‑76 (MQ=255) cTGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATc > 1:7048591/1‑76 (MQ=255) tGACTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCTTGGTTCCGCCGATTTATCg > 1:7866369/1‑76 (MQ=255) cTCATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTg > 2:3701069/1‑76 (MQ=255) cATAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATACAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCg > 1:474023/1‑76 (MQ=255) tAAGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGc > 2:462460/1‑76 (MQ=255) aGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCaa > 1:6302475/1‑76 (MQ=255) aGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCaa < 1:7183196/76‑1 (MQ=255) aGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCaa < 1:5300275/76‑1 (MQ=255) aGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCaa < 1:7599559/76‑1 (MQ=255) aGGTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCaa > 1:3983557/1‑76 (MQ=255) ggTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCaaa < 2:539036/76‑1 (MQ=255) gTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAAt < 2:6183013/76‑1 (MQ=255) gTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAAt > 1:7520206/1‑76 (MQ=255) gTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAAt < 1:4319732/76‑1 (MQ=255) gTGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAACCTGGTTCCGCCGATTTATCGCTGCGGCaaaa > 2:1439617/1‑75 (MQ=255) tGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATa < 2:387112/76‑1 (MQ=255) tGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATa < 1:4507313/76‑1 (MQ=255) tGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATa < 1:4634988/76‑1 (MQ=255) tGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATa < 1:5950278/76‑1 (MQ=255) tGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATa < 1:6491678/76‑1 (MQ=255) tGGCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATa < 1:7814713/76‑1 (MQ=255) gCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTccgc > 2:124619/1‑52 (MQ=255) gCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTccgc < 1:124619/52‑1 (MQ=255) gCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAAc < 2:5840077/76‑1 (MQ=255) gCTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAAc < 1:1563446/76‑1 (MQ=255) cTCCTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACg < 1:5721999/76‑1 (MQ=255) ccTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCa > 1:7722204/1‑76 (MQ=255) ccTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCa > 1:847623/1‑76 (MQ=255) ccTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCa < 2:4810209/76‑1 (MQ=255) cTACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAg < 1:3343616/76‑1 (MQ=255) tACCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGc < 2:5973274/76‑1 (MQ=255) aCCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCg > 1:6573985/1‑76 (MQ=255) aCCTGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCg > 2:7976682/1‑76 (MQ=255) | TCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCG > REL606/2762355‑2762500 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |