| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,911,997 | (A)6→5 | intergenic (‑198/‑125) | yifB ← / → ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,911,992 | 0 | A | . | 97.1% | 156.5 / ‑1.2 | 34 | intergenic (‑193/‑130) | yifB/ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base . (18/15); minor base T (1/0); total (19/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01 | |||||||||||
ACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTA > REL606/3911917‑3912064 | aCAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTa < 1:814099/76‑1 (MQ=255) aaaGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTaaa > 2:870352/1‑76 (MQ=255) aGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTaaaaa < 2:106626/76‑1 (MQ=255) aTGAACTACGAGGAAGGGAGCAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTaaaaacaa > 1:1030326/1‑73 (MQ=255) gAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAacaaca < 1:889122/76‑1 (MQ=255) aaCTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAcaacaa < 2:1161790/76‑1 (MQ=255) aCTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAACAACAAt < 1:44872/76‑1 (MQ=255) cTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAACAACAAtt < 1:385533/76‑1 (MQ=255) tACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAACAACAAttt < 1:919698/76‑1 (MQ=255) aCGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAACAACAATTta > 1:1208125/1‑76 (MQ=255) gAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAACAACAATTtata > 1:1027666/1‑76 (MQ=255) gAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAACAACAATTtata < 2:255134/76‑1 (MQ=255) aGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTAATTTT‑AAAAACAACTNTTTatat < 2:983498/76‑1 (MQ=255) gAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAACAACAATTTATATTGAAatt > 1:415814/1‑76 (MQ=255) aacaacATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAACAACAATTTATATTGAAatta > 2:598509/1‑76 (MQ=255) aTTCAACCTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAACAACAATTTATATTGAAATTATTAAAc > 1:1114566/1‑76 (MQ=255) ttCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAACAACAATTTATATTGAAATTATTAAACg < 2:642606/76‑1 (MQ=255) aTACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAACAACAATTTATATTGAAATTATTAAACGcat < 1:358327/76‑1 (MQ=255) tACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAACAACAATTTATATTGAAATTATTAAACGcatc > 2:867145/1‑76 (MQ=255) aCTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAACAACAATTTATATTGAAATTATTAAACGcatca < 2:631062/76‑1 (MQ=255) cTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAACAACAATTTATATTGAAATTATTAAACGcatcat < 1:470054/76‑1 (MQ=255) tGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAACAACAATTTATATTGAAATTATTAAACGCATCATa > 1:1026090/1‑76 (MQ=255) gAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAACAACAATTTATATTGAAATTATTAAACGCATCATaa > 1:631204/1‑76 (MQ=255) gAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAACAACAATTTATATTGAAATTATTAAACGCATCATaa < 1:698860/76‑1 (MQ=255) gAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAACAACAATTTATATTGAAATTATTAAACGCATCATaa < 1:829244/76‑1 (MQ=255) aaTTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaaa < 2:747887/76‑1 (MQ=255) tGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATc > 1:424374/1‑76 (MQ=255) ttttttCACTCACTATTTTATTTTT‑AAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCa > 1:1097531/1‑76 (MQ=255) tttttCACTCACTATTTTATTTTT‑AAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaa > 2:1041988/1‑76 (MQ=255) ttttCACTCACTATTTTATTTTT‑AAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaaa > 1:422968/1‑76 (MQ=255) tcactcacTATTTTATTTTT‑AAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaaaaaa > 2:70035/1‑76 (MQ=255) cactcactATTTTATTTTT‑AAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAat > 2:1018552/1‑76 (MQ=255) ttttattttT‑AAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTAc > 1:950226/1‑76 (MQ=255) ttttattttT‑AAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTAc < 1:748884/76‑1 (MQ=255) tttattttT‑AAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACt > 2:593181/1‑76 (MQ=255) tttattttT‑AAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACt > 1:581346/1‑76 (MQ=255) ttattttT‑AAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTa > 1:664014/1‑76 (MQ=255) tattttTAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTAt > 2:1070789/3‑76 (MQ=255) ttttAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTa < 1:711332/76‑1 (MQ=255) ttttAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTa < 1:554869/76‑1 (MQ=255) ttttAAAAACAACAATTTATATAGAAANTANTGNACGCATCNTACAAACCGGCCAAAAAATATCTTGTACTATTTa < 1:1052711/76‑1 (MQ=255) | ACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTA > REL606/3911917‑3912064 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |