Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	REL606	475,062	Δ4 bp	coding (241‑244/1677 nt)	ybaL ←	predicted transporter with NAD(P)‑binding Rossmann‑fold domain

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	REL606	475062	475065	4	39 [0]	[0] 39	ybaL	predicted transporter with NAD(P)‑binding Rossmann‑fold domain

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 475061	0 (0.000)	39 (1.040)	30/148	0.3	100%	coding (245/1677 nt)	ybaL	predicted transporter with NAD(P)‑binding Rossmann‑fold domain
	?	REL606	475066 =	0 (0.000)					coding (240/1677 nt)	ybaL	predicted transporter with NAD(P)‑binding Rossmann‑fold domain

GCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGGCGATGGCCTTTACCGCCATCAAATCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCG  >  REL606/474990‑475133                                                                        |                                                                         gcgcCATCCCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGGCGATGGCCTTTACCGCCAatcc                                                                      <  2:1017460‑M1/76‑5 (MQ=255)  cgcCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGGCGATGGCCTTTACCGCCAatcct                                                                     <  2:804948‑M1/76‑6 (MQ=255)  cgcCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGGCGATGGCCTTTACCANCAatcct                                                                     <  2:972458‑M1/76‑6 (MQ=255)   gcCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGGCGATGGCCTTTACCGCCAatcctt                                                                    <  2:302913‑M1/76‑7 (MQ=255)    ccATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGGCGATGGCCTTTACCGCCAatccttc                                                                   >  1:694302‑M1/1‑69 (MQ=255)    ccATACCAACCCGCGTCGCCCCGTCTATCTGGGCGATCGCACCGGGAATGGCGATGGCCTTTACCGCCAatccttc                                                                   <  2:744618‑M1/76‑8 (MQ=255)     cATCCCCCGCATCTTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGGCGATGGCCTTTACCGCCAatccttca                                                                  <  2:1010463‑M1/76‑9 (MQ=255)     cATCCCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGGCGATGGCCTTTACCGCCAatccttca                                                                  <  2:480358‑M1/76‑9 (MQ=255)     cATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGGCGATGGCCTTTACCGCCAatccttca                                                                  <  1:284837‑M1/76‑9 (MQ=255)         cccAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGGCGATGGCCTTTACTGCCAatccttcagcga                                                              >  1:681366‑M1/1‑64 (MQ=255)           cagcagCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGGCGATGGCCTTTACCGCCAatccttcagcgaaa                                                            >  2:597426‑M1/1‑62 (MQ=255)              cagcGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGGCGATGGCCTTTACCGCCAatccttcagcgaaaagg                                                         >  2:693968‑M1/1‑59 (MQ=255)              cagcGGCGCCACGGCTATCTGGGCGATCGCACGGGGAATGGCGACGGCNCTTTCCGCCAatccttcagcgaaaagt                                                         <  2:1057‑M1/76‑18 (MQ=255)               agcGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGGCGATGGCCTTTACCGCCAatccttcagcgaaaagtg                                                        >  1:660038‑M1/1‑58 (MQ=255)               agcGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGGCGATGGCCTTTACCGCCAatccttcagcgaaaagtg                                                        <  1:787012‑M1/76‑19 (MQ=255)               agcGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGGCGATGGCCTTTACCGCCAatccttcagcgaaaagtg                                                        <  2:234985‑M1/76‑19 (MQ=255)               agcGTCGCCACGGCTATCTGGGCGATCGCACCGGGAAAGGCGATGGCCTTTACCGCCAatccttcagcgaaaagtg                                                        >  1:1020707‑M1/1‑58 (MQ=255)                  gTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGGNGATGGCCTTTACCGCCAatccttcagcgaaaagtgcaa                                                     >  1:1175511‑M1/1‑55 (MQ=255)                  gTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGGCGATGGCCTTTACCGCCAatccttcagcgagaagtgcaa                                                     <  1:1124317‑M1/76‑22 (MQ=255)                       cACGGCTATCTAGGCGATCGCACCGGGAATGGCGATGGCCTTTACCGCCAatccttcagcgaaaagtgcaaaccga                                                >  1:66319‑M1/1‑50 (MQ=255)                         cgtctatCGGGGCGAGCGCACCGGGAATGGCGATGGCCTTTACCGCCAatccttcagcgaaaagtgcaaaccgacg                                              <  1:538163‑M1/73‑29 (MQ=255)                           gCTATCGGGTCGATCGCCCCGGGAATGGCGATGGCCTTTACCGCCAatccttcagcgaaaantgnaaaccgacgcc                                            >  2:190634‑M1/1‑46 (MQ=255)                            cTATCTGGGCGATCGCACCGGGAATGGCGATGGCCTTTACCGCCAatccttcagcgaaaagtgcaaaccgacgcca                                           >  1:843711‑M1/1‑45 (MQ=255)                                cTGGGCGATCGCACCGGGAATGGCGATGGCCTTTACCGCCAatccttcagcgaaaagtgcaaaccgacgccaaaca                                       >  1:363983‑M1/1‑41 (MQ=255)                                cTGGGCGATCGCACCGGGAATGGCGATGGCCTTTACCGCCAatccatcagcgaaaagtgcaaaccgacgccaaaca                                       >  2:806617‑M1/1‑41 (MQ=255)                                     cGATCGCACCGGGAATGGCGATGGCCTTTACCGCCAatgcttcagcgaaaagtgcaaaccgacgccaaacatcaac                                  <  1:1017049‑M1/76‑41 (MQ=255)                                     cGATCGCACCGGGAATGGCGATGGCCTTTACCGCCAatccttcagcgaaaagtgcaaaccgacgccaaacatcaac                                  >  2:1154922‑M1/1‑36 (MQ=255)                                          gCACCGGGAATGGCGATGGCCTTTACCGCCAatccttcagcgaaaagtgcaaaccgacgcccaacatcaaccgaat                             <  1:83325‑M1/76‑46 (MQ=255)                                          gCACCGGGAATGGCGATGGCCTTTACCGCCAatccttcagcgaaaagtgcaaaccgacgccaaacatcaacagaat                             >  2:537684‑M1/1‑31 (MQ=255)                                          gCACCGGGAATGGCGATGGCCTTTACCGCCAatccttcagcgaaaagtgcaaaccgacgccaaacatcaacagaat                             >  1:814934‑M1/1‑31 (MQ=255)                                             ccGGGAATGGCGATGGCCTTTACCGCCAatccttcagcgaaaagtgcaaaccgacgccaaacatcaacagaatgac                          <  1:287495‑M1/76‑49 (MQ=255)                                                  aaTGGCGATGGCCTTTACCGCCAatccttcagcgaaaagtgcaaaccgacgccaaacatcaacagaatgacgccca                     >  2:474871‑M1/1‑23 (MQ=255)                                                  aaTGGCGATGGCCTTTACCGCCAatccttcagcgaaaagtgcaaaccgacgccaaacatcaacagaatgacgccca                     >  2:607353‑M1/1‑23 (MQ=255)                                                   aTGGCGACGGTCTTTACCTCCAatcctgcagcgcaaagtnnaaaccgangccaaacatcaacagaatgacgcccag                    <  1:1192252‑M1/76‑55 (MQ=255)                                                        gATGGCCTTTACCGCCAatccttcagcgaaaagtgcaaaccgacgccaaacatcaacagaatgacgcccagttcag               >  2:259623‑M1/1‑17 (MQ=255)                                                                ttACCGCCAatccttcagcgaaaagtgcaaaccgacgccaaacatcaacagaatgacgcccagttcagccagttcc       >  2:316348‑M1/1‑9 (MQ=255)                                                                 tACCGCCAatccttcagcgaaaagtgcaaaccgacgccaaacatcaacagaatgacgcccagttcagccagttccg      <  2:351845‑M1/76‑69 (MQ=255)                                                                    cGCCAatccttcagcgaaaagggcaaancgacgccnnacatcaacagaatgacgcccagttcagcaagatccgggg   >  1:1188423‑M1/1‑5 (MQ=255)                                                                     cccaatccttcagcgaaaagtgcaaaccgacgccaaacatcaacagaatgacgcccagttcagccagttccggggc  <  2:376332‑M1/75‑73 (MQ=255)                                                                        |                                                                         GCGCCATACCCAGCAGCGTCGCCACGGCTATCTGGGCGATCGCACCGGGAATGGCGATGGCCTTTACCGCCATCAAATCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCG  >  REL606/474990‑475133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑