| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,145,984 | A→T | I41F (ATC→TTC) | rpoD → | RNA polymerase sigma factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,145,984 | 0 | A | T | 96.3% | 95.4 / NA | 27 | I41F (ATC→TTC) | rpoD | RNA polymerase sigma factor |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (14/12); minor base G (0/1); total (14/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAAGGAGCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATC > REL606/3145911‑3146056 | tAAGGAGCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCagatcagttc < 1:1149492/76‑1 (MQ=255) aaGGAACAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGTTCg > 1:1139204/1‑76 (MQ=255) aGCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGTTCGAAGa < 1:981656/76‑1 (MQ=255) gCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGTTCGAAGAc < 1:660304/76‑1 (MQ=255) cAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGTTCGAAGAca < 1:1154238/76‑1 (MQ=255) cTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGTTCGAAGACatcatc > 2:897824/1‑76 (MQ=255) aTCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGTCCGAAGACATCATCCa < 1:110340/76‑1 (MQ=255) gACCTATGCCGAGGTCAATGACCTTATGCCGGAAGATATNGTCGAATCAGATCAGNTCGAAGTCATCATCCaaacg > 2:1197837/1‑74 (MQ=255) gACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGTTCGAGGACATCATCCAAATg > 1:7881/1‑76 (MQ=255) gACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGTTCGAAGACATCATCCAAATg > 1:589437/1‑76 (MQ=255) gCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGTTCGAAGACATCATCCAAATGATCAACg < 1:574708/76‑1 (MQ=255) gAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGTTCGAAGACATCATCCAAATGATCAACGACa > 1:613221/1‑76 (MQ=255) ggTCAATGACCATCTGCGGTAAGATATCGTCGATTCAGATCAGTTCGAAGATATCATCCAAATGATCAACGACATg < 1:55055/76‑1 (MQ=255) ttaCCATCTGCCGGAAGATATCGTCGATTCAGATCAGTTCGAAGACATCATCCAAATGATCAACGACATGGGCAtt < 2:1093761/74‑1 (MQ=255) tGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGTTCGAAGACATCATCCAAATGATCAACGACATGGGCAtt > 2:60980/1‑76 (MQ=255) gACCATCTGCCGGAAGATATCGTCGATTCAGATCAGTTCGAAGACATCATCCAAATGATCAACGACATGGGCATTc < 2:294036/76‑1 (MQ=255) aCCATCTGNCGGGAGATATCGTCGTTTGAGATCTGGTCGAAGACATCATCCAAATGATCAACGACATGGGCATTCa < 2:202583/76‑1 (MQ=255) tCTGCCGGAAGATATCGACGATGCAGATCAGTTCGAAGACATCATCCAAATGATCCACGACATGGGCATTCAGGTg < 1:55966/76‑1 (MQ=255) gCCGGAAGATATCGTCGATTCAGATCAGTTCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATg > 2:639356/1‑76 (MQ=255) ccGGAAGATATCGTCGATTCAGATCAGTTCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATgg > 1:782006/1‑76 (MQ=255) cGGAAGATATCGTCGATTCAGATCAGTTCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGga > 2:1003205/1‑76 (MQ=255) ggAAGATATCGTCGATTCAGATCAGTTCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGgaa > 2:1132719/1‑76 (MQ=255) aaGATATCGTCGATTCAGATCAGTTCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGgaaga < 2:756585/76‑1 (MQ=255) gATATCGTCGATTCAGATCAGTTCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGTTGATGGaagaag < 2:48148/76‑1 (MQ=255) atatCGTCGATTCAGATCAGTTCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATNGAAGAAGc < 1:1167822/76‑1 (MQ=255) tatCGTCGATTCAGATCAGTTCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCa > 1:976058/1‑76 (MQ=255) cgtcgATTCAGATCAGTTCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCg > 1:575462/1‑76 (MQ=255) cgtcgATTCAGAGCACTTCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGNTGGAAGAAGCACCg > 1:1038328/1‑76 (MQ=255) cgATTCAGATCAGTTCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGAt > 2:165716/1‑76 (MQ=255) ttCAGATCAGTTCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGcc > 2:379855/1‑76 (MQ=255) atcagttcGAAGACATCATCCAAATGATCAACGACATNGGCATTCAGGTGATGGAAGAAGCACCGGATGCCgatga > 1:1185766/1‑76 (MQ=255) atcagttcGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCgatga < 2:1112352/76‑1 (MQ=255) tcagttcGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACAGGATGCCgatgat > 2:555448/1‑76 (MQ=255) cagttcGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATc > 1:735573/1‑76 (MQ=255) | TAAGGAGCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATC > REL606/3145911‑3146056 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |