Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,193,779	G→T	L143I (CTT→ATT)	yeiB ←	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,193,779	0	G	T	100.0%	104.3 / NA	31	L143I (CTT→ATT)	yeiB	conserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/17);  total (14/17)

CCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCC  >  REL606/2193707‑2193850                                                                         |                                                                        ccGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAAAGCCAATccc                                                                      >  2:947658/1‑76 (MQ=255)  cGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATcccc                                                                     <  2:92800/76‑1 (MQ=255)   gATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAATTCAGCCCCAATAACAGCAAAACGCCAATCcaca                                                                    <  1:1109492/76‑1 (MQ=255)    aTGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccac                                                                   <  1:954268/76‑1 (MQ=255)     tGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccacc                                                                  <  1:448357/76‑1 (MQ=255)      gCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccacca                                                                 <  1:728690/76‑1 (MQ=255)       cATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAg                                                                >  2:350060/1‑76 (MQ=255)         tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGAt                                                              <  2:508083/76‑1 (MQ=255)           cGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaa                                                            <  1:524094/76‑1 (MQ=255)            ggCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaaa                                                           >  2:937584/1‑76 (MQ=255)               gTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCa                                                        >  1:908092/1‑76 (MQ=255)                tCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCAt                                                       >  2:149748/1‑76 (MQ=255)                 ccAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATg                                                      <  1:658255/76‑1 (MQ=255)                  cAGGCGCGGCTGGTCTGACTGTCAGCAATCAGCCCCAATNACAGCCAAACGCCAANCCCCACCAGATAAAGCATGa                                                     >  2:1198022/1‑76 (MQ=255)                           cTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt                                            >  1:635668/1‑76 (MQ=255)                           cTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt                                            <  1:830869/76‑1 (MQ=255)                            tGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                           <  1:595171/76‑1 (MQ=255)                             ggTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                          >  2:151449/1‑76 (MQ=255)                             ggTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                          <  2:298911/76‑1 (MQ=255)                               tCTGGCTGACGGAAATCAGTCCCAATAACAGCAAAGCGCCAATCCCCACCAGAAAAAGCACGACGCCTGTCTTaaa                                        >  2:16862/1‑76 (MQ=255)                                 tGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa                                      >  1:544335/1‑76 (MQ=255)                                  ggCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg                                     >  1:138606/1‑76 (MQ=255)                                    cTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGc                                   >  2:531207/1‑76 (MQ=255)                                      gTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                 <  2:844902/76‑1 (MQ=255)                                      gTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                 >  2:847782/1‑76 (MQ=255)                                       tCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt                                <  1:263132/76‑1 (MQ=255)                                         cgAAATCAGCCACAACACCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                              <  2:1065070/75‑1 (MQ=255)                                          gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                             <  2:585772/76‑1 (MQ=255)                                               cAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt                        >  2:786645/1‑76 (MQ=255)                                                             gCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAc          >  2:386982/1‑76 (MQ=255)                                                                  aCGCCAATCCCCACCAGATNANGCATGACNCCTGTATTAAACACGCTTTTTNCCNATGGCGCATCGCGCACCAgcc     <  2:170672/76‑1 (MQ=255)                                                                  aCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgcc     <  2:1005041/76‑1 (MQ=255)                                                                    gCCAATCCCCACCAGATAAAGCATGACGCCTGTATTGAACAGGCTTTTTACCGATCGCGCATCGCGCACCAgccgc   >  2:964503/1‑76 (MQ=255)                                                                     ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc  >  2:788934/1‑76 (MQ=255)                                                                         |                                                                        CCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCC  >  REL606/2193707‑2193850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑