Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,295,476	A→C	*85C (TGA→TGC)	yfaE →	predicted 2Fe‑2S cluster‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,295,476	0	A	C	100.0%	266.2 / NA	63	*85C (TGA→TGC)	yfaE	predicted 2Fe‑2S cluster‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (32/31);  total (32/31)

AGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATAC  >  REL606/2295404‑2295548                                                                         |                                                                         aGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGCAtt                                                                       <  1:573863/76‑1 (MQ=255) aGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGCAtt                                                                       <  1:701955/76‑1 (MQ=255) aGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGCAtt                                                                       >  2:709040/1‑76 (MQ=255)    cTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGCATTGGt                                                                    >  1:504039/1‑76 (MQ=255)     tttATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGCATTGGtt                                                                   <  2:896547/76‑1 (MQ=255)     tttATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGCATTGGtt                                                                   <  1:812251/76‑1 (MQ=255)      ttATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGCATTGGTTg                                                                  >  2:137872/1‑76 (MQ=255)       tATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGCATTGGTTGt                                                                 <  2:722972/76‑1 (MQ=255)       tATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGCATTGGTTGt                                                                 >  1:1010258/1‑76 (MQ=255)       tATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGCATTGGTTGt                                                                 >  1:312600/1‑76 (MQ=255)       tATTCAGCCGGGGGAAATTTTGCCCTGTNTTTGCCGGGCAAAAGGCGACANTGCAATCGAGATGTGCATTGGTTGt                                                                 >  1:171343/1‑76 (MQ=255)        aTTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGCATTGGTTGTa                                                                <  2:624292/76‑1 (MQ=255)         ttCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGCATTGGTTGTAg                                                               <  1:700018/76‑1 (MQ=255)          tCAGCCGGGGGAAATTTNGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATCGGTTGTAGt                                                              >  1:152816/1‑76 (MQ=255)               cGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGCATTGGTTGTAGTGCCAg                                                         <  2:889930/76‑1 (MQ=255)                 ggggAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGCATTGGTTGTAGTGCCAGAt                                                       <  1:416891/76‑1 (MQ=255)                  gggAAATATTGCCCTGTTGTTGCCGGGCAAGAGGCGATATTGAAATCGAGATGTGCATTGGTTGTAGTGCCAGATa                                                      <  1:24577/76‑1 (MQ=255)                    gAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGCATTGGTTGTAGTGCCAGATACa                                                    <  1:804614/76‑1 (MQ=255)                     aaaTTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGCATTGGTTGTAGTGCCAGATACaa                                                   >  1:284592/1‑76 (MQ=255)                      aaTTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGCATTGGTTGTAGTGCCAGATACAAc                                                  >  2:778954/1‑76 (MQ=255)                         tttGCCCTGTTGTTGCCTTGCAAAAGGCGATATTGAAATCGAGATGTGCATTGGTTGTAGTNCCNGATACAACGCt                                               >  2:192752/1‑76 (MQ=255)                         tttGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGCATTGGTTGTAGTGCCAGATACAACGCt                                               <  2:318870/76‑1 (MQ=255)                           tGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGCATTGGTTGTAGTGCCAGATACAACGCTTa                                             <  2:429987/76‑1 (MQ=255)                            gCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGCATTGGTTGTAGTGCCAGATACAACGCTTAt                                            <  2:229520/76‑1 (MQ=255)                             cccTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGCATTGGTTGTAGTGCCAGATACAACGCTTATg                                           <  1:346521/76‑1 (MQ=255)                              ccTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGCATTGGTTGTAGTGCCAGATACAACGCTTATGc                                          <  1:524441/76‑1 (MQ=255)                                tgttgtTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGCATTGGTTGTAGTGCCAGATACAAAGCTTATGCGt                                        >  1:663890/1‑76 (MQ=255)                                 gttgttGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGCATTGGTTGTAGTGCCAGATACAACGCTTATGCGTc                                       >  1:629317/1‑76 (MQ=255)                                    gttgCCGGGCAAACGGCGATNTTGAAATCGTGATGTNCATTGGTTGTAGTACGAGATATAACGCTTATGCGTCTTa                                    <  2:1204640/76‑1 (MQ=255)                                    gttgCCGGGCAAAAGGCGATATTGAAATCGAGATGTGCATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTa                                    <  1:916031/76‑1 (MQ=255)                                    gttgCCGGGCAAAAGGCGATATTGAAATCGAGATGTGCATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTa                                    <  2:957811/76‑1 (MQ=255)                                       gCCGGGCAAAAGGCGATATTGAAATCGAGATGTGCATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCt                                 <  1:430726/76‑1 (MQ=255)                                       gCCGGGCAAAAGGCGATATTGAAATCGAGATGTGCATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCt                                 <  1:302804/76‑1 (MQ=255)                                       gCCGGGCAAAAGGCGATATTGAAATCGAGATGTGCATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCt                                 <  1:44581/76‑1 (MQ=255)                                       gCCGGGCAAAAGGCGATATTGAAATCGAGATGTGCATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCt                                 <  1:469056/76‑1 (MQ=255)                                           ggCAAAAGGCGATATTGAAATCGAGATGTGCATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGcc                             <  2:451820/76‑1 (MQ=255)                                             cAAAAGGCGATATTGAAATCGAGATGTGCATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTa                           <  2:1020786/76‑1 (MQ=255)                                             cAAAAGGCGATATTGAAATCGAGATGTGCATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTa                           >  2:844174/1‑76 (MQ=255)                                             cAAAAGGCGATATTGAAATCGAGATGTGCATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTa                           <  1:709040/76‑1 (MQ=255)                                             cAAAAGGCGATATTGAAATCGAGATGTGCATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTa                           >  2:844153/1‑76 (MQ=255)                                             cAAAAGGCGATATTGAAATCGAGATGTGCATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTa                           >  2:536602/1‑76 (MQ=255)                                              aaaaGGCGATATTGAAATCGAGATGTGCATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTAc                          >  2:791869/1‑76 (MQ=255)                                              aaaaGGCGATATTGAAATCGAGATGTGCATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTAc                          >  2:351256/1‑76 (MQ=255)                                               aaaGGCGNAATTGAAATCGAGATGTGCATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACa                         >  2:981149/1‑76 (MQ=255)                                                aaGGCGATATTGAAATCGAGATGTGCATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACaa                        >  2:554270/1‑76 (MQ=255)                                                aaGGCGATATTGAAATCGAGATGTGCATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACaa                        >  2:910614/1‑76 (MQ=255)                                                aaGGCGATATTGAAATCGAGATGTGCATTGGTTGTAGTGCCAGATACAACGCCTATCCGTCTTATCTGGGCTCCaa                        >  1:707237/1‑76 (MQ=255)                                                  ggCGATATTGAAATCGAGATGTGCATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACg                      <  2:630996/76‑1 (MQ=255)                                                      atatTGAAATCGAGATGTGCATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTa                  <  2:1077176/76‑1 (MQ=255)                                                      atatTGAAATCGAGATGTGCATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTa                  <  2:709923/76‑1 (MQ=255)                                                        atTGAAAGCGAGATGTGCATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACa                <  2:591456/76‑1 (MQ=255)                                                         ttGAAATCGAGATGTGCATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACAt               >  2:85488/1‑76 (MQ=255)                                                          tGAAATCGAGATGTGCATTGGTTATAGTGCCAGATACAACTCATATGCTTCATATCTGGCCTAGAACNATTACATg              >  2:196728/1‑76 (MQ=255)                                                           gAAATCGAGATGTGCATTTGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATgg             >  1:1143040/1‑76 (MQ=255)                                                           gAAATCGAGATGTGCATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATNTGGCCTACAANGATTACATgg             <  2:1191711/76‑1 (MQ=255)                                                            aaaTCGAGATGTGCATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGc            >  2:633471/1‑76 (MQ=255)                                                                cGAGATGTGCATTGGTTGTCGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCgtag        >  1:1030070/1‑76 (MQ=255)                                                                cGAGATGTGCATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTTTCTCGCCCACAACGATTACATGGCggag        >  1:680340/1‑76 (MQ=255)                                                                cGAGATGTGCATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCgtag        >  2:857700/1‑76 (MQ=255)                                                                cGAGATGTGCATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCgtag        >  1:1123661/1‑76 (MQ=255)                                                                 gagaTGTGCATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCgtagt       >  2:634395/1‑76 (MQ=255)                                                                 gagaTGTGCATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCgtagt       >  2:340379/1‑76 (MQ=255)                                                                  agaTGTGCATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCgtagta      >  2:1057353/1‑76 (MQ=255)                                                                   gaTGTGCATTGGTTGTAGTGCCAAATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTaa     >  1:388365/1‑76 (MQ=255)                                                                      gtgCATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATAc  <  2:790841/76‑1 (MQ=255)                                                                         |                                                                         AGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATAC  >  REL606/2295404‑2295548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑