| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,911,972 | (T)7→8 | intergenic (‑173/‑150) | yifB ← / → ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,911,965 | 1 | . | T | 97.0% | 670.2 / 9.6 | 167 | intergenic (‑166/‑157) | yifB/ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
| Reads supporting (aligned to +/- strand): ref base . (1/4); new base T (46/116); total (47/120) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 190 total aligned reads displayed.
GAATGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAA > REL606/3911891‑3912035 | gAATGATGCTCGCAAAAATGCAGCGGCCAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑t < 2:6508141/76‑1 (MQ=255)gAATGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑t < 1:6132185/76‑1 (MQ=255)gAATGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑t < 1:3562760/76‑1 (MQ=255) attatgCTCGCAACAAGTCAGCGGACAAAGGTTTAACTACGAGGAAGGGAACAACATTCATACTGAaatttaa‑ttt < 2:4676495/73‑1 (MQ=255) atgatgCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑ttt < 1:7831440/76‑1 (MQ=255) atgatgCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑ttt < 2:7114965/76‑1 (MQ=255) tgatgCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑tttt < 2:7239841/76‑1 (MQ=255) atgCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑tttt‑tt < 2:35415/76‑1 (MQ=255) atgCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑tttt‑tt > 2:3736576/1‑76 (MQ=255) tgCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑tttt‑ttt < 2:5191962/76‑1 (MQ=255) gCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑tttt‑tttt > 1:5036643/1‑75 (MQ=255) gCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑tttt‑tttt > 1:4007966/1‑75 (MQ=255) gCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑tttt‑tttt > 2:5639675/1‑75 (MQ=255) cTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑tttt‑ttttc < 1:6563562/76‑3 (MQ=255) tCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑tttt‑ttttca < 2:6291156/76‑4 (MQ=255) cAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTtcactc < 1:2835533/76‑1 (MQ=255) cAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTtcactc > 2:3311660/1‑76 (MQ=255) cAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTtcactc < 2:2956290/76‑1 (MQ=255) aaaaaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTtcactca < 2:5911496/76‑1 (MQ=255) aaaaaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTtcactca < 1:7153287/76‑1 (MQ=255) aaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACta < 2:2999251/76‑1 (MQ=255) aaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACta < 2:1194214/76‑1 (MQ=255) aaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACta < 1:5457192/76‑1 (MQ=255) aTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACtat < 2:5727791/76‑1 (MQ=255) aTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACtat < 1:8003093/76‑1 (MQ=255) tGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACtatt < 1:5053518/76‑1 (MQ=255) tGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACtatt > 2:1791357/1‑76 (MQ=255) gCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACtattt < 1:1640529/76‑1 (MQ=255) gCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACtattt < 1:4042694/76‑1 (MQ=255) cAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACtatttt < 2:5017044/76‑1 (MQ=255) cAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACtatttt < 1:6793724/76‑1 (MQ=255) cAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACtatttt < 1:115315/76‑1 (MQ=255) gCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACtattttat < 1:5954097/76‑1 (MQ=255) gCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACtattttat < 1:1314155/76‑1 (MQ=255) cGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACtattttatt < 2:2829605/76‑1 (MQ=255) gACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACTattttatttt < 2:158691/76‑1 (MQ=255) gACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACTattttatttt < 1:981820/76‑1 (MQ=255) aaaaaGATGAACTACGAAGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTa < 1:3092175/75‑1 (MQ=255) aaGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACtattttatt > 2:2608532/1‑70 (MQ=255) aaGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTaaa > 2:2240421/1‑76 (MQ=255) aaGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTaaa < 1:6899516/76‑1 (MQ=255) aaGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTaaa < 2:7366003/76‑1 (MQ=255) aGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTaaaa > 2:6677235/1‑76 (MQ=255) aTGAACTACGAGGAAGGTAACAACATTCATACGGTAATTGCA‑TTTTGTTTCTCACACTATTTTATTTTTAAAAaac < 2:4505872/76‑1 (MQ=255) aTGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAaac < 2:5543736/76‑1 (MQ=255) aTGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAaac < 1:6219127/76‑1 (MQ=255) aTGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAaac < 1:6748400/76‑1 (MQ=255) tGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAaaca > 1:4287826/1‑76 (MQ=255) gAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAaacaa < 2:1936724/76‑1 (MQ=255) aaCTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAaacaac < 2:4703996/76‑1 (MQ=255) tACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACTGTTTTATTTTTAAAAAACAACAAt < 2:7828717/76‑1 (MQ=255) tACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAAt > 2:6762065/1‑76 (MQ=255) aCGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAAtt > 2:6794037/1‑76 (MQ=255) cGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAAttt < 1:3366197/76‑1 (MQ=255) cGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAAttt < 1:5932194/76‑1 (MQ=255) gAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTta < 2:2814478/76‑1 (MQ=255) gAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTta < 2:2709791/76‑1 (MQ=255) gAGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTta < 2:8086655/76‑1 (MQ=255) aGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTtat < 1:3694034/76‑1 (MQ=255) aGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTtat < 1:2967974/76‑1 (MQ=255) aGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTtat > 2:5765491/1‑76 (MQ=255) aGGAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTtat < 2:6160680/76‑1 (MQ=255) gAAGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTTatat < 2:6997746/76‑1 (MQ=255) aaGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATAtt < 1:5119281/76‑1 (MQ=255) aaGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATAtt < 1:1772615/76‑1 (MQ=255) aGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTg > 2:6912784/1‑76 (MQ=255) aGGGAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTg > 1:3901148/1‑76 (MQ=255) gggAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGa > 2:3444763/1‑76 (MQ=255) gggAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGa < 2:4468656/76‑1 (MQ=255) ggAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGaa < 1:1932519/76‑1 (MQ=255) ggAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGaa < 2:6551037/76‑1 (MQ=255) gAACAACATTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAGCAATTTATATTGaaa < 1:765783/76‑1 (MQ=255) aacaacATTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAat < 1:7804680/76‑1 (MQ=255) acaacaTTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAatt < 1:6121020/76‑1 (MQ=255) acaacaTTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAata < 2:5383138/76‑2 (MQ=255) caacaTTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAatta > 1:5872263/1‑76 (MQ=255) caacaTTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAatta > 1:6656638/1‑76 (MQ=255) aacaTTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAattat < 1:2899370/76‑1 (MQ=255) aacaTTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAattat < 2:6123785/76‑1 (MQ=255) aacaTTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAattat > 1:2995684/1‑76 (MQ=255) acaTTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAattatt < 1:6321212/76‑1 (MQ=255) acaTTCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAattatt > 1:6406447/1‑76 (MQ=255) ttCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTaaa < 1:5538480/76‑1 (MQ=255) ttCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTaaa < 2:1466279/76‑1 (MQ=255) tCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAAc > 2:3674683/1‑76 (MQ=255) tCATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAAc > 1:1097775/1‑76 (MQ=255) cATACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACg < 1:5614404/76‑1 (MQ=255) aTACTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGc < 1:233618/76‑1 (MQ=255) cTGAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcatc > 1:115852/1‑76 (MQ=255) gAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcatcat > 1:2590722/1‑76 (MQ=255) gAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcatcat < 1:4653544/76‑1 (MQ=255) gAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcatcat < 2:4753609/76‑1 (MQ=255) gAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcatcat < 1:8025017/76‑1 (MQ=255) gAAATTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcatcat < 1:7759491/76‑1 (MQ=255) aaaTTGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATa < 2:4228362/76‑1 (MQ=255) aTTGAA‑TTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaaa > 2:107664/1‑76 (MQ=255) ttGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaaa > 2:3024407/1‑76 (MQ=255) ttGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaaa < 1:4440558/76‑1 (MQ=255) ttGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaaa < 2:390123/76‑1 (MQ=255) ttGAATTTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaaa < 1:6006931/76‑1 (MQ=255) | GAATGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTT‑TTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAA > REL606/3911891‑3912035 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |