BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	REL606	3,894,997	Δ6,934 bp	IS150‑mediated	rbsD–[yieO]	rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	REL606	3893608–3894994	3901930	6937–8323	101 [99]	[1] 312	[insJ‑5]–[yieO]	[insJ‑5],insK‑5,rbsD,rbsA,rbsC,rbsB,rbsK,rbsR,[yieO]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 3652533	NA (NA)	291 (1.220)	113/146	0.1	100%	noncoding (1443/1443 nt)	IS150	repeat region
*	?	REL606	3901931 =	0 (0.000)	291 (1.220)	113/146	0.1	100%	coding (489/1428 nt)	yieO	predicted multidrug or homocysteine efflux system

Only 100 of 101 total aligned reads displayed.

AGGTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAA  >  REL606/3893532‑3893627

aGGTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCaa                      <  1:6885184/76‑1 (MQ=255)

aGGTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCaa                      <  1:2287221/76‑1 (MQ=255)

 ggTCGAAATCTAACGCCAGACTGTACTGCTCCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCaaa                     <  1:1779344/76‑1 (MQ=255)

 ggTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCaaa                     <  2:5665553/76‑1 (MQ=255)

 ggTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCaaa                     >  1:8110433/1‑76 (MQ=255)

 ggTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCaaa                     <  2:7141081/76‑1 (MQ=255)

 ggTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCaaa                     <  1:3193998/76‑1 (MQ=255)

 ggTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCaaa                     <  2:572331/76‑1 (MQ=255)

 ggTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCaaa                     <  1:1694616/76‑1 (MQ=255)

 ggTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCaaa                     <  1:1575810/76‑1 (MQ=255)

  gTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAg                    <  1:6022059/76‑1 (MQ=255)

  gTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAg                    <  2:1235924/76‑1 (MQ=255)

  gTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAg                    <  1:7392824/76‑1 (MQ=255)

  gTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAg                    <  2:4676955/76‑1 (MQ=255)

  gTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAg                    <  1:7710479/76‑1 (MQ=255)

   tCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGc                   <  2:7899579/76‑1 (MQ=255)

    cGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGcc                  >  2:2518145/1‑76 (MQ=255)

    cGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGcc                  <  1:3860671/76‑1 (MQ=255)

    cGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGcc                  <  1:3842088/76‑1 (MQ=255)

    cGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGcc                  <  2:710136/76‑1 (MQ=255)

    cGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGcc                  <  1:95607/76‑1 (MQ=255)

    cGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGcc                  <  1:917315/76‑1 (MQ=255)

     gAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCa                 <  1:6003335/76‑1 (MQ=255)

     gAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCa                 <  2:7603202/76‑1 (MQ=255)

     gAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCa                 >  2:2832183/1‑76 (MQ=255)

     gAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCa                 <  1:214155/76‑1 (MQ=255)

     gAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCa                 <  1:7036721/76‑1 (MQ=255)

     gAAATCAAACGCAAGTCTGTACTGCACCCATTTTGTTGGACAATGAAATGGAATAGCCCCTAATATGTCAAAGCCa                 <  1:6232514/76‑1 (MQ=14)

      aaaTCTACCGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCaa                <  2:4478477/76‑1 (MQ=255)

      aaaTCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCACAGCCaa                >  2:5866185/1‑76 (MQ=255)

      aaaTCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCaa                >  2:4692457/1‑76 (MQ=255)

      aaaTCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCaa                <  1:63827/76‑1 (MQ=255)

      aaaTCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCaa                <  1:5808431/76‑1 (MQ=255)

       aaTCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCaaa               <  2:3595368/76‑1 (MQ=255)

       aaTCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCaaa               <  2:3149619/76‑1 (MQ=255)

       aaTCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCaaa               <  2:5625707/76‑1 (MQ=255)

        aTCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCaaaa              <  1:6345570/76‑1 (MQ=255)

         tCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAAt             <  1:1222353/76‑1 (MQ=255)

         tCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAAt             <  2:312444/76‑1 (MQ=255)

         tCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAAt             >  1:5408109/1‑76 (MQ=255)

         tCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAAt             <  2:3049091/76‑1 (MQ=255)

         tCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAAt             >  1:5505403/1‑76 (MQ=255)

         tCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAAt             <  1:2971346/76‑1 (MQ=255)

          cTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATa            <  2:7756166/76‑1 (MQ=255)

          cTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATa            <  2:1054606/76‑1 (MQ=255)

          cTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATa            >  2:6161609/1‑76 (MQ=255)

          cTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATa            >  2:4793592/1‑76 (MQ=255)

           tAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATAc           <  1:2376948/76‑1 (MQ=255)

           tAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATAc           >  1:1627201/1‑76 (MQ=255)

           tAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATAc           <  1:6107063/76‑1 (MQ=255)

           tAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATAc           <  1:5172281/76‑1 (MQ=255)

            aaCGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATAcc          <  1:2814739/76‑1 (MQ=255)

            aaCGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATAcc          >  1:4811123/1‑76 (MQ=255)

            aaCGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATAcc          <  1:1841651/76‑1 (MQ=255)

            aaCGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATAcc          >  2:17382/1‑76 (MQ=255)

            aaCGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATAcc          <  1:5932694/76‑1 (MQ=255)

             aCGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATAccc         >  2:1876620/1‑76 (MQ=255)

               gCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCtt       <  1:2851362/76‑1 (MQ=39)

               gCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCtt       <  1:3453509/76‑1 (MQ=39)

                ccAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCttt      >  2:5711279/1‑76 (MQ=38)

                ccAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCttt      >  2:2814123/1‑76 (MQ=38)

                ccAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCttt      >  2:706557/1‑76 (MQ=38)

                ccAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCttt      >  1:21391/1‑76 (MQ=38)

                ccAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCttt      >  2:7741107/1‑76 (MQ=38)

                 cAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCtttt     <  2:5250600/76‑1 (MQ=35)

                  aGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTg    <  2:659889/76‑1 (MQ=34)

                  aGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTg    <  1:3857175/76‑1 (MQ=34)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCttttaa   >  1:6402953/1‑74 (MQ=17)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   >  1:7978085/1‑76 (MQ=32)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   >  2:7903562/1‑76 (MQ=32)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   >  2:7833482/1‑76 (MQ=32)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   >  2:7726998/1‑76 (MQ=32)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   >  2:7550518/1‑76 (MQ=32)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   >  2:7461811/1‑76 (MQ=32)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   <  2:7339563/76‑1 (MQ=32)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   <  2:7245175/76‑1 (MQ=32)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   >  2:6249801/1‑76 (MQ=32)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   >  2:7111814/1‑76 (MQ=32)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   >  2:7077170/1‑76 (MQ=32)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   >  1:4293015/1‑76 (MQ=32)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   >  1:1183870/1‑76 (MQ=32)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   >  1:1501847/1‑76 (MQ=32)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   >  1:1554350/1‑76 (MQ=32)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   >  1:1631624/1‑76 (MQ=32)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   >  1:2239407/1‑76 (MQ=32)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   >  1:2281625/1‑76 (MQ=32)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   >  1:2675105/1‑76 (MQ=32)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   >  1:2699949/1‑76 (MQ=32)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   >  1:3895658/1‑76 (MQ=32)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   <  2:4872094/76‑1 (MQ=32)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   >  1:4559977/1‑76 (MQ=32)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   >  1:5046677/1‑76 (MQ=32)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   >  1:7055938/1‑76 (MQ=32)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   <  1:1079459/76‑1 (MQ=32)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   >  1:8063482/1‑76 (MQ=32)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   >  2:2104310/1‑76 (MQ=32)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   >  2:3537958/1‑76 (MQ=32)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   >  2:3927400/1‑76 (MQ=32)

                   aaCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   >  2:3948925/2‑76 (MQ=14)

                    aCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGaa  <  1:274841/76‑1 (MQ=31)

AGGTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAA  >  REL606/3893532‑3893627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`