BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	REL606	3,228,601	Δ557 bp		[yraM]	[yraM]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	REL606	3228601	3229157	557	274 [2]	[3] 276	[yraM]	[yraM]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 3228600	2 (0.010)	236 (1.080)	99/134	0.1	99.0%	intergenic (‑31/‑34)	yraL/yraM	predicted methyltransferase/hypothetical protein
*	?	REL606	3229158 =	3 (0.010)	236 (1.080)	99/134	0.1	99.0%	coding (525/2037 nt)	yraM	hypothetical protein

Only 100 of 276 total aligned reads displayed.

TTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTCGGCGCGAAAGAAAAGCAGCAGAATATTGACGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGATCAACGCCGACGAAAATATT  >  REL606/3229083‑3229233

taaagctggccctgagaattatccgccgattggtgttgtttcattgtgtcgtccgtattgccgatttaatattgac                                                                             <  2:5722750‑M2/1‑1 (MQ=255)

  aagctggccctgagaattatccgccgattggtgttgtttcattgtgtcgtccgtattgccgatttaatattgaCGc                                                                           <  1:5079488‑M2/3‑1 (MQ=255)

  aagctggccctgagaattatccgccgattggtgttgtttcattgtgtcgtccgtattgccgatttaatattgaCGc                                                                           <  1:388464‑M2/3‑1 (MQ=255)

   agctggccgtgagaattatccgccgattggtgttgtttcattgtgtcgtccgtattgccgatttaatattgaCGcc                                                                          <  2:1521406‑M2/4‑1 (MQ=255)

   agctggccctgagaattatccgccgattggtgttgtttcattgtgtcgtccgtattgccgatttaatattgaCGcc                                                                          <  1:5212852‑M2/4‑1 (MQ=255)

    gctggccctgagaattatccgccgattggtgttgtttcattgtgtcgtccgtattgccgatttaatattgaCGCCa                                                                         <  1:2962346‑M2/5‑1 (MQ=255)

     ctggccctgagaattatccgccgattggtgttgtttcattgtgtcgtccgtattgccgatttaatattgaCGCCAc                                                                        >  2:4835492‑M2/71‑76 (MQ=255)

      tggccctgagaattatccgccgattggtgttgtttcattgtgtcgtccgtattgccgatttaatattgaCGCCAcc                                                                       <  1:4881701‑M2/7‑1 (MQ=255)

       ggccctgagaattatccgccgattggtgttgtttcattgtgtcgtccgtattgccgatttaatattgaCGCCACCt                                                                      <  1:4654730‑M2/8‑1 (MQ=255)

        gccctgagaattatccgccgattggtgttgtttcattgtgtcgtccgtattgccgatttaatattgaCGCCACCTg                                                                     <  1:900642‑M2/9‑1 (MQ=255)

         ccctgagaattatccgccgattggtgttgtttcattgtgtcgtccgtattgccgatttaatattgaCGCCACCTgg                                                                    <  1:7625500‑M2/10‑1 (MQ=255)

             gagaattatccgccgattggtgttgtttcattgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAgg                                                                <  2:3326795‑M2/14‑1 (MQ=255)

             gagaattatccgccgattggtgttgtttcattgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAgg                                                                <  2:5648905‑M2/14‑1 (MQ=255)

              agaattatccgccgattggtgttgtttcattgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGgc                                                               <  2:6663548‑M2/15‑1 (MQ=255)

              agaattatccgccgattggtgttgtttcattgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGgc                                                               >  1:7969009‑M2/62‑76 (MQ=255)

                 attatccgccgattggggttgtttcattgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGct                                                            >  1:1600415‑M2/59‑76 (MQ=255)

                  ttatccgccgattggtgttgtttcattgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGctc                                                           >  1:993076‑M2/58‑76 (MQ=255)

                  ttatccgccgattggtgttgtttcattgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGctc                                                           <  1:6966345‑M2/19‑1 (MQ=255)

                    atccgccgattggtgttgtttcattgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCtctc                                                         <  1:5999187‑M2/21‑1 (MQ=255)

                         ccgattggtgttgtttcattgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCa                                                    >  1:7218723‑M2/51‑76 (MQ=255)

                          cgattggtgttgtttcattgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCAt                                                   <  1:6673635‑M2/27‑1 (MQ=255)

                          cgattggtgttgtttcattgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCAt                                                   <  1:1226975‑M2/27‑1 (MQ=255)

                          cgattggtgttgtttcattgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCAt                                                   <  1:8078703‑M2/27‑1 (MQ=255)

                           tGCTCAGGAACCGCTGCTCGGCGCGAAAGAAAAGCAGCAGAATATTGACGCCACCTGGCAGGCGCTCTCCTCCATg                                                  >  2:7108334/1‑76 (MQ=255)

                           gattggtgttgtttcattgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATg                                                  <  2:7811930‑M2/28‑1 (MQ=255)

                           gattggtgttgtttcattgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATg                                                  <  1:1053445‑M2/28‑1 (MQ=255)

                              tggtgttgtttcattgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACt                                               >  1:840272‑M2/46‑76 (MQ=255)

                              tggtgttgtttcattgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACt                                               >  1:5885790‑M2/46‑76 (MQ=255)

                              tggtgttgtttcattgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACt                                               >  2:6483760‑M2/46‑76 (MQ=255)

                              tggtgttgtttcattgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACt                                               >  1:5014733‑M2/46‑76 (MQ=255)

                                 tgttgtttcattgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAg                                            <  2:369820‑M2/34‑1 (MQ=255)

                                 tgttgtttcattgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAg                                            <  2:524743‑M2/34‑1 (MQ=255)

                                 tgttgtttcattgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAg                                            >  1:6103143‑M2/43‑76 (MQ=255)

                                  gttgtttcattgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAgg                                           >  2:4356162‑M2/42‑76 (MQ=255)

                                  gttgtttcattgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAgg                                           >  1:6820747‑M2/42‑76 (MQ=255)

                                   ttgtttcattgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGa                                          <  1:6485940‑M2/36‑1 (MQ=255)

                                    tgtttcattgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGaa                                         <  2:2146675‑M2/37‑1 (MQ=255)

                                    tgtttcattgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGaa                                         >  2:3563486‑M2/40‑76 (MQ=255)

                                    tgtttcattgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGaa                                         >  1:5629940‑M2/40‑76 (MQ=255)

                                     gtttcattgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCCTGACTCAGGAAc                                        >  1:3839398‑M2/39‑76 (MQ=255)

                                     gtttcattgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAAc                                        <  1:5719727‑M2/38‑1 (MQ=255)

                                     gtttcattgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAAc                                        <  2:4545906‑M2/38‑1 (MQ=255)

                                      tttcattgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACa                                       >  2:8005998‑M2/38‑76 (MQ=255)

                                       ttcattgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGaaccg                                      >  1:36263‑M2/37‑74 (MQ=255)

                                       ttcattgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAg                                      >  2:5089111‑M2/37‑76 (MQ=255)

                                         cattgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGc                                    <  2:117476‑M2/42‑1 (MQ=255)

                                         cattgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGc                                    >  2:5490795‑M2/35‑76 (MQ=255)

                                         cattgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGc                                    >  1:103008‑M2/35‑76 (MQ=255)

                                         cattgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGc                                    >  1:3375096‑M2/35‑76 (MQ=255)

                                          attgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCg                                   <  1:245959‑M2/43‑1 (MQ=255)

                                          attgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCg                                   <  2:3506898‑M2/43‑1 (MQ=255)

                                          attgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCg                                   >  1:7062297‑M2/34‑76 (MQ=255)

                                          attgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCg                                   >  1:2016238‑M2/34‑76 (MQ=255)

                                          attgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCg                                   <  1:1517604‑M2/43‑1 (MQ=255)

                                          attgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCg                                   <  2:8139313‑M2/43‑1 (MQ=255)

                                          attgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAAAAGGCg                                   >  2:743002‑M2/34‑76 (MQ=255)

                                           ttgtgtcgtccgtattgccgatttaatattgaCGCAACCTGGCAGGAGCACTCCTCCATGACTCAGGACCAGGCGa                                  >  2:6121745‑M2/33‑76 (MQ=255)

                                            tgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCgac                                 >  2:2487525‑M2/32‑75 (MQ=255)

                                            tgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGaa                                 >  1:4245398‑M2/32‑76 (MQ=255)

                                            tgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGaa                                 >  2:2740601‑M2/32‑76 (MQ=255)

                                            tgtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGaa                                 >  1:5833206‑M2/32‑76 (MQ=255)

                                             ttgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAAt                                <  2:7218723‑M2/46‑1 (MQ=255)

                                             gtgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAAt                                <  1:2867425‑M2/46‑1 (MQ=255)

                                              tgtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATa                               <  1:5632294‑M2/47‑1 (MQ=255)

                                               gtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCCTGACTCAGGAACAGGCGAAAAc                              >  2:5586563‑M2/29‑76 (MQ=255)

                                               gtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGaatcc                              >  1:6313069‑M2/29‑74 (MQ=255)

                                               gtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATAc                              >  1:8025833‑M2/29‑76 (MQ=255)

                                               gtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATAc                              <  2:4238540‑M2/48‑1 (MQ=255)

                                               gtcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATAc                              <  1:3032506‑M2/48‑1 (MQ=255)

                                                tcgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACg                             <  1:3739623‑M2/49‑1 (MQ=255)

                                                 cgtccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGc                            <  2:7650374‑M2/50‑1 (MQ=255)

                                                    ccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTgg                         >  2:3019741‑M2/24‑76 (MQ=255)

                                                    ccgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTgg                         >  2:6820164‑M2/24‑76 (MQ=255)

                                                     cgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGt                        >  1:3491647‑M2/23‑76 (MQ=255)

                                                     cgtattgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGt                        <  1:4035763‑M2/54‑1 (MQ=255)

                                                        attgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGAt                     <  2:4910800‑M2/57‑1 (MQ=255)

                                                          tgccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGATCa                   <  2:4035132‑M2/59‑1 (MQ=255)

                                                           tccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGATCaa                  <  2:1388276‑M2/60‑1 (MQ=255)

                                                            ccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGATCAAc                 <  1:7402955‑M2/61‑1 (MQ=255)

                                                            ccgatttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGATCAAc                 >  2:4776589‑M2/16‑76 (MQ=255)

                                                               atttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGATCAACGcc              >  2:4443468‑M2/13‑76 (MQ=255)

                                                               atttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGATCAAAGcc              >  1:2626673‑M2/13‑76 (MQ=255)

                                                                 ttaatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGCGATCAAAGccgc            >  1:7399828‑M2/11‑75 (MQ=255)

                                                                  taatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGGGATCAACGCcgac           >  1:1273627‑M2/10‑76 (MQ=255)

                                                                   aatattgaCGCCAGCTGGCAGGCGCTCTCCTCCATGACTCAGGAGCAGGCGAATACGCTGGTGAACAACGCcgacg          <  2:1889619‑M2/68‑1 (MQ=255)

                                                                   aatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGATCAACGCcgacg          >  2:1824216‑M2/9‑76 (MQ=255)

                                                                   aatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGATCAACGCcgacg          <  2:5891522‑M2/68‑1 (MQ=255)

                                                                   aatattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGATCAACGCcgacg          >  2:75318‑M2/9‑76 (MQ=255)

                                                                    atattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGATCAACGCcgacga         <  1:2736176‑M2/69‑1 (MQ=255)

                                                                     tattgaCGCCCCCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGATCAACGCCGACGaa        <  2:4311027‑M2/70‑1 (MQ=255)

                                                                     tattgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGATCAACGCCGACGaa        <  1:7241471‑M2/70‑1 (MQ=255)

                                                                      attgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGATCAACGCCGACGaaa       <  2:166442‑M2/71‑1 (MQ=255)

                                                                      attgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGATCAACGCCGACGaaa       <  1:7860896‑M2/71‑1 (MQ=255)

                                                                        tgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGATCAACGCCGACGAAAat     <  1:3248972‑M2/73‑1 (MQ=255)

                                                                        tgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGATCAACGCCGACGAAAat     <  1:6513070‑M2/73‑1 (MQ=255)

                                                                        tgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGATCAACGCCGACGAAAat     >  1:2346947‑M2/4‑76 (MQ=255)

                                                                        tgaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGATCAACGCCGACGAAAat     <  1:2050084‑M2/73‑1 (MQ=255)

                                                                         gaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGATCAACGCCGACGAAAata    >  2:640031‑M2/3‑76 (MQ=255)

                                                                         gaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGATCAACGCCGACGAAAata    >  2:7678583‑M2/3‑76 (MQ=255)

                                                                         gaCGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGATCAACGCCGACGAAAata    >  1:7451395‑M2/3‑76 (MQ=255)

TTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTCGGCGCGAAAGAAAAGCAGCAGAATATTGACGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGATCAACGCCGACGAAAATATT  >  REL606/3229083‑3229233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`