| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,853,067 | 0 | A | C | 36.0% | 238.5 / 93.4 | 150 | T56P (ACC→CCC) | yeaN | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base A (91/5); new base C (2/52); total (93/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.19e-32 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 194 total aligned reads displayed.
ACCGGCGCAGCGCCGTTACTGGATACGATTCGTTCCG‑CTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATGGAACGTA > REL606/1852992‑1853142 | aCCGGCGCAGCGCCGTTACTGGATACGATTCGTTCCG‑CTTACTCGCTGACGACAGCGCAAACCGGCTTATTGacca < 1:2582035/76‑1 (MQ=255)aCCGGCGCAGCGCCGTTACTGGATACGATTCGTTCCG‑CTTACTCGCTGACGACAGCGCAAAACGGCCTATTGacca > 1:2098650/1‑76 (MQ=255) ccGGCGCAGCGCCGTTACTGGATACGATTCGTTCCG‑CTTACTCGCTGACGACAGCGCAAACCGGCTTATTGaccac < 2:7554600/76‑1 (MQ=255) cGGCGCAGCGCCGTTACTGGATACGATTCGTTCCG‑CTTACTCGCTGACGACAGCGCAAACCGGCTTATTGaccacc > 1:3934572/1‑76 (MQ=255) cGGCGCAGCGCCGTTACTGGATAAGATTCGTTCCG‑CTTACTCGCCGGCGACAACGCAAACAGGGTTATTTaccaac > 2:967407/1‑74 (MQ=255) gcgcAGCGCCGTTACTGGATACGATTCGTTCCG‑CTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCt < 2:4347901/76‑1 (MQ=255) cgcAGCGCCGTTACTGGATACGATTCGTTCCG‑CTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTg < 2:518993/76‑1 (MQ=255) cAGCGCCGTTACTGGATACGATTCGTTCCG‑CTTACTCGCTGACGACAGCGCAAACCGGCTTTTTGACCACCCTGcc > 1:2044365/1‑76 (MQ=255) cAGCCCCGTTACTGGATACGATTCGTTCCG‑CTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGcc < 2:2257754/76‑1 (MQ=255) aGCGCCGTTACTGGATACGATTCGTTCCG‑CTTACTCGCTGACCACAACGCAAACCCGCTTATTGAACCCCCTGCCa > 2:7707021/1‑76 (MQ=255) gcgcCGTTACTGGATAAGATTCGTTCCG‑CTTATTCGCTGACTACACCGCAAATCGGCTTATTGACAACCCTGCCat > 2:3626819/1‑76 (MQ=255) gcCGTTACTGGATACGATTTCTTTCG‑CTTACTCGCTGACGACAACGCAAACCGGCATATTGTCCACCCCGCCatta > 2:6978425/1‑76 (MQ=255) ccGTTACTGGATACGATTCGTTCCG‑CTTACTCGCTGACGACAGCGCAAACCGGCTTATTTACCACCCTGCCattat > 2:4745366/1‑76 (MQ=255) gTTACTGGATACGATTCGTTCCG‑CTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTg > 1:973969/1‑76 (MQ=255) gTTACGGGATACGCTCCGTCCCG‑CTTCCCCGCTGACGACAGCGCAAACCGGCTTATTGCCCCCCCTGCCATTATTg < 1:375013/76‑1 (MQ=255) ttACTGGATACGTTTCGTTCCG‑TTTCCTCGCGGACGACAGCGCAACCCGGCTTTTTGCCCCCCCTGCCATTATTgg < 2:7325488/76‑1 (MQ=255) tagtGGATACGTTTCGTCCCG‑TTTCCTCGCGGACGACAGCACAACCCGGTTTTTTGCCCCCCCTGCCATTATTGGc < 2:6488412/73‑1 (MQ=255) tACTGGATACGATTCGTTCCG‑CTTACTCGCTGGCGACAGAGCAAACCGGCTTATTGACCACCCTGCCCTTATTTGc > 2:5575869/1‑76 (MQ=255) tACTGGATACGATTCGTTCCG‑CTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCCTTATTGGc > 2:3696617/1‑76 (MQ=255) tACTGGATACGATTCGTTCCG‑CTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCAACCTGCCATTATTGGc > 1:3261642/1‑76 (MQ=255) aCTGGATACGATTCGTTCCG‑CTTACTCGCTGACGACAGCGCAAACCGGCTTCTTGACCCCCCTGCCATTATTTGcc > 2:507987/1‑76 (MQ=255) cTGGATACGATTCGTTCCG‑CTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCt > 1:7899869/1‑76 (MQ=255) tGGATACGATTCGTTCCG‑CTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTTGCCtt > 2:6642299/1‑76 (MQ=255) tGGATACGATTCGTTCCG‑CTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCtt > 2:6062203/1‑76 (MQ=255) gggATCCTATTCTTTCCG‑TTTACGCGCTGACGACAGCGCAACCCGGCTTATTGCCCCCCCTGCCATTATTGGCCtt < 2:6897022/75‑1 (MQ=255) ggATACGTTTCGTCCCG‑CTTCCTCGCTGACGACAGCGCAAACCGGTTTATTGCCCCCCCTGCCATTATTGGCCttt < 1:1841093/76‑1 (MQ=255) ggATACGATTCGTTCCG‑CTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCttt > 1:1079559/1‑76 (MQ=255) ggATACGATTCGTTCCG‑CTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCttt > 1:4391048/1‑76 (MQ=255) gATCCGATT‑GTTCCGCCTTCCTCGCTGACGACAGCACAACCCGGTTTTTTAACCCCCCTGCCATTATTGGCCTTTg < 1:853212/76‑1 (MQ=255) gATACGTTTCGTTCCG‑CTTCCTCGCTGACGACAGCGCAACCCGGCTTATTGCCCCCCCTGCCATTATTGGCCTTTg < 2:1225057/76‑1 (MQ=255) cGATTCGTTCCG‑CTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCt > 2:5290704/1‑76 (MQ=255) gATTCGTTCCG‑CTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTa > 2:4770327/1‑76 (MQ=255) gATTCGTTCCG‑CTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTa > 2:5429795/1‑76 (MQ=255) gATTCGTCCCG‑CTTACTCGCTGACGACAGCGCAACCCGGTTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTa < 2:204361/76‑1 (MQ=255) aTTCGTTCCG‑CTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTaa > 2:5438706/1‑76 (MQ=255) aTTCGTTCCG‑CTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTaa > 1:5701172/1‑76 (MQ=255) tccGTTCCG‑CTTCCTCGCTGACGACAGCGCAAACCGGCTTATTGACCCCCCTGCCATTATTGGCCTTTGCGCTAAt < 1:6060584/74‑1 (MQ=255) cGTTCCG‑CTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAAtct > 2:1506690/1‑76 (MQ=255) gTTCCG‑CTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAAtctc > 2:619872/1‑76 (MQ=255) gTTCCG‑CTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAAtctc > 2:2097559/1‑76 (MQ=255) ttCCG‑CTTACTCGCTGACGACAGCGCAAACCGGCTTATTGAACACCCTGCCATTATTGGCCTTTGCGCTAATCTCa > 2:7793264/1‑76 (MQ=255) tcccG‑TTTCCTCGCTGACGACAGCGCAAACCGGTTTATTGACCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCa < 2:4202893/74‑1 (MQ=255) tCCG‑CTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCAc > 1:5206064/1‑76 (MQ=255) tCCG‑CTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCT‑ATCTCAcc > 2:2227030/1‑76 (MQ=255) cccG‑TTTACTCGCGGACGACAGCCCAACCCGGCTTATTCCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCAc < 2:4404199/75‑1 (MQ=255) ctttACTCGCCGGCGACAGCGCAACCGGGTTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCtt < 2:2652334/74‑1 (MQ=255) cTTCCTCGCTGACGACAGCGCAACCCCGCTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCttt < 1:505497/76‑1 (MQ=255) cTTCCTCGCTGACGACAGCCCAACCCGGCTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCttt < 1:936981/76‑1 (MQ=255) cTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCttt > 2:3084121/1‑76 (MQ=255) cTTACCCGCTGACAACAGCGCAACCCGTCTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCttt < 2:1366958/76‑1 (MQ=255) ttACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTg > 2:6457614/1‑76 (MQ=255) ttACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTg > 2:6528806/1‑76 (MQ=255) tACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCtttcg > 2:300364/1‑74 (MQ=255) tACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTgg > 1:6519825/1‑76 (MQ=255) tACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTgg > 1:5140483/1‑76 (MQ=255) tACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTgg > 2:4847198/1‑76 (MQ=255) aCTCGCTGACGACAGGGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGc > 2:1112013/1‑76 (MQ=255) aCTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGc > 2:2380607/1‑76 (MQ=255) aCTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGc > 2:816107/1‑76 (MQ=255) cTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCt > 2:4895231/1‑76 (MQ=255) tCGCTGACGACAGCGCAAACCGGCTTATTGACCCCCCTGCCATTATTGGCCTTTGCGCAACTCTCACCTTTGGCTg > 2:5963358/1‑76 (MQ=255) ccGCTGACGACAGCGCAAACCGGCTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTg < 2:7104046/75‑1 (MQ=255) cTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGccc > 1:555028/1‑76 (MQ=255) tGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGcccc > 2:3263040/1‑76 (MQ=255) tGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGcccc > 2:1610514/1‑76 (MQ=255) gccgacAGCGCAACCCGCCTTATCCCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCg < 1:4590651/74‑1 (MQ=255) gacgacAGCGCAAACGGAGTAATTGCCCACCCTGCCATTATTGGCCTTTGCGCTAATCTCCTCTCTGGCTGCCTCg > 2:3817234/1‑76 (MQ=255) gacgacAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGacccc > 2:3017754/1‑75 (MQ=255) gacgacAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCg > 2:40461/1‑76 (MQ=255) gacgacAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCg > 1:5401322/1‑76 (MQ=255) gacgacAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCg > 2:5352415/1‑76 (MQ=255) cgacAGCACAACCCGGCTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGt < 2:7001028/76‑1 (MQ=255) gacAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTg > 2:1443848/1‑76 (MQ=255) cgccgcAACCCGGTTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGgc < 2:793704/73‑1 (MQ=255) cAGCGCAACCCGGTTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGgc < 1:1094163/76‑1 (MQ=255) aGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGgcg > 1:8022242/1‑76 (MQ=255) aGCGAAACCCGGTTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGgcg < 2:2758311/76‑1 (MQ=255) cgcAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGcgcg > 1:6633611/1‑76 (MQ=255) cgcAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGcgcg > 1:7686260/1‑76 (MQ=255) cAAGCCGTCTTATTACCCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGAc < 1:3652900/76‑1 (MQ=255) aaaCCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACg > 2:694522/1‑76 (MQ=255) gCTTATCGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTgg < 1:1360565/76‑1 (MQ=255) ttATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTa > 2:1918856/1‑76 (MQ=255) tATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTAt > 1:7573577/1‑76 (MQ=255) tATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTAt > 1:4890638/1‑76 (MQ=255) tATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTAt > 1:3056848/1‑76 (MQ=255) tATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTCTGGCTGCCCCGGTGGCGCGACGTTTTGGTAt > 1:7901634/1‑76 (MQ=255) tATTAACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTAt > 1:3586427/1‑76 (MQ=255) tttGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATg < 2:3473475/75‑1 (MQ=255) aTTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATg > 1:5839592/1‑76 (MQ=255) tGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCTCGACGTTTTGGTATGGa > 1:2831907/1‑76 (MQ=255) tGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATGGa > 2:4550309/1‑76 (MQ=255) tGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATGGa > 2:1744628/1‑76 (MQ=255) tGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATGGa > 2:7035739/1‑76 (MQ=255) tGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATGGa > 1:3957610/1‑76 (MQ=255) tGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATGGa > 1:7235444/1‑76 (MQ=255) gACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATGGaa > 2:1847858/1‑76 (MQ=255) accaccCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATGGAAc > 2:286114/1‑76 (MQ=255) accCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATGGAACGTa > 1:689149/1‑76 (MQ=255) accCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATGGAACGTa > 1:1640135/1‑76 (MQ=255) | ACCGGCGCAGCGCCGTTACTGGATACGATTCGTTCCG‑CTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATGGAACGTA > REL606/1852992‑1853142 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |