| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,356,014 | 0 | T | G | 35.2% | 329.2 / 49.5 | 177 | T58P (ACC→CCC) | yfbU | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (18/96); new base G (62/0); total (81/96) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.44e-31 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 237 total aligned reads displayed.
GCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTG > REL606/2355939‑2356089 | gCTGGGTCTGCAAATTAGAACAGGAAACATGCGACGCACGATACAGCTCCATAATGTCGATGATAGTCGGGCCGGt > 2:5152978/1‑76 (MQ=255) ggTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTttc < 2:6894522/76‑1 (MQ=255) gTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTttct < 1:6379398/76‑1 (MQ=255) tCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTttctt < 2:7902280/76‑1 (MQ=255) tGCAAATTAGACCAGGAAACATGCAACGCGTGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTcttctt < 2:8019841/76‑1 (MQ=255) tGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGGtttttttt > 2:5084609/1‑76 (MQ=255) tGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGGTTattctt > 1:1715633/1‑76 (MQ=255) gCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGGTTCtttttt > 1:5068837/1‑76 (MQ=255) cAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTc < 2:2196340/76‑1 (MQ=255) cAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTc < 2:5941717/76‑1 (MQ=255) cAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTc < 2:4455767/76‑1 (MQ=255) cAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGGTTTTTCtttt > 1:2268954/1‑75 (MQ=255) aaaTTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGGTTTTTCTTTCa > 2:3193969/1‑76 (MQ=255) aaTTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTTAg > 1:6107892/1‑76 (MQ=255) aaTTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAg < 2:752752/76‑1 (MQ=255) ttAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGGTTCTTTTTTTAGCt > 1:5371383/1‑76 (MQ=255) tAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGGCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTc < 1:5844197/76‑1 (MQ=255) aGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTTAGATCg > 2:4495588/1‑76 (MQ=255) aGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCg > 2:3889922/1‑76 (MQ=255) aGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCg < 2:495140/76‑1 (MQ=255) aGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGGTTTTTTTTTTAGCTcc > 1:6904312/1‑75 (MQ=255) gACCAGGAAACACGCGACGCGTGATACATCTCCATAATGTCGCTGCTGGCGCGTCAGGTATTTTTGTTCCGCTCGc > 2:1482723/1‑76 (MQ=255) ccAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTTAACTCGCCa > 1:7999109/1‑76 (MQ=255) ccAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCa < 2:2537933/76‑1 (MQ=255) ccAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGGTTTTTCTTTTAGCTCGCCa > 2:7044548/1‑76 (MQ=255) cAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCaa > 2:4135978/1‑76 (MQ=255) aGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCaaa < 1:1411695/76‑1 (MQ=255) aGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGGTTGTTCTTTCCACTCGCCcaa > 1:1577226/1‑76 (MQ=255) ggAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGGTTCTTTTTTTAGCTCGCCAAAc > 2:4679092/1‑76 (MQ=255) gAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACt < 1:105037/76‑1 (MQ=255) aaaCATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGGTTCTTCTTTTAGCTCGCCAAACTc > 2:1306818/1‑76 (MQ=255) cATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTcgcg < 1:7109709/76‑1 (MQ=255) cATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGGTTCTTTTTTCAGCTCGCCCAAATcgcc > 1:7910721/1‑75 (MQ=255) aTGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGa < 2:7242011/76‑1 (MQ=255) aTGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGGTTCTTCTTTCCACTCGCCACACCcgcgc > 2:7292366/1‑75 (MQ=255) tGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTTTTCTTTCAGCTCGCCAACATcgcggt > 2:7704366/1‑74 (MQ=255) tGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGAt < 1:2555726/76‑1 (MQ=255) cAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATcc < 2:6958609/76‑1 (MQ=255) aaCGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGGTTCTTTTTTTAGCTCGCCCCACTCGCGATCCa > 1:6506742/1‑76 (MQ=255) aCGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATccca > 2:1363587/1‑74 (MQ=255) aCGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCaa < 2:7918684/76‑1 (MQ=255) cGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAAt < 2:213814/76‑1 (MQ=255) gCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGGTTTTTCTTTCAGCTCCCCAAAACCGCGATCCAAtt > 1:7173620/1‑76 (MQ=255) gaTGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTc < 2:7974801/75‑1 (MQ=255) cATGATACATCTCCATAATGTCGATGATAGTGCGGCATGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTc < 1:2052920/76‑1 (MQ=255) cATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTc < 1:4958512/76‑1 (MQ=255) cATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGGTTCTTCTTTCAGCTCGCCCAACTCCCGATCCAATTc > 2:3560578/1‑76 (MQ=255) aTGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTTTTCTTTTCGCTCGCCAAACACCCGATCCACTTCa > 2:3017859/1‑76 (MQ=255) aTGATACATCTCCATAATGTCGATGATAGTGCGGCAGGGTTCTTTTTTCAGCTCGCCAAACTCGCGATCCAAATCa > 1:4825273/1‑76 (MQ=255) tGATACATCTCCATAATGTCGATGATAGTGCGGCAGGGTTCTTCTTTCAGCTCGCCAAACTCGCGCTCCAATTTAc > 2:186876/1‑76 (MQ=255) gATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACg < 2:2632961/76‑1 (MQ=255) gATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACg > 2:2030583/1‑76 (MQ=255) gATACATCTCCATAATGTCGATGATAGTGCGGCAGGGTTCTTCTTTCAGCTCCCCAAACTCGCGCTCCAAATCAcc > 1:824591/1‑75 (MQ=255) aCATCTCCATAATGTCGATGATAGTGCGGCAGGGTTCTTCTTTCAGCTCGCCAAACTCGCGATCCCATTCACgact > 2:1816928/1‑73 (MQ=255) aTCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCt < 1:5183672/76‑1 (MQ=255) tctcCATAATGTCGATGATAGTGCGGCAGGTTTTTTCTTTCAACTCGCCAAAATCGCGATCCCATTCCCGCCTCTg > 1:4750573/1‑76 (MQ=255) ctcCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGt < 1:3678190/76‑1 (MQ=255) ctcCATAATGTCGATGATAGTGCGGCAGGGTTCTTCTTTCAGCTCGCCCAACTCGCGATCCCAATCACCCACCTGt > 2:5407099/1‑76 (MQ=255) ctcCATAATGTCGATGATAGTGCGGCAGGGTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCCTCTGt > 1:5786814/1‑76 (MQ=255) tcCATAATGTCGATGATAGTGCGGCAGGGTTTTTCTTTTAGCCCGCCCAAACCGAGAGCCAAATCACGCGTCTTTa > 1:3194046/1‑76 (MQ=255) tcCATAATGTCGATGATAGTGCGGCAGGGTTCTTCTTTCCGCTCGCCAAACTCGCGAATCAATTCACGCATCTGTa > 1:6928582/1‑76 (MQ=255) ccATAATGTCGATGATAGTGCGGCAGGTTTTTTCTTTCAGCTCCCCAAACTCGCGATCCAATTCACGCATCTGTaa > 1:842282/1‑76 (MQ=255) ccATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTaa < 1:5615431/76‑1 (MQ=255) ccATAATGTCGATGATAGTGCGGCAGGGTTTTTCTTTCCGCTCGGCCAACTCGCGATCCAATTTACGCCTCTGTaa > 2:1633482/1‑76 (MQ=255) ccATAATGTCGATGATAGTGCGGCAGGGTTTTTCTTTCCGCTCGCCCAAATCGCGATCCAATTCACGCATCTGTaa > 1:4272668/1‑76 (MQ=255) cATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAAt < 2:3973971/76‑1 (MQ=255) cATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAAt < 2:1515963/76‑1 (MQ=255) cATAATGTCGATGATAGTGCGGCAGGGTTTTTTTTTCAGCTCGCCAAACTCGCGCTCCAATTCACGCATCTGTAAt > 1:7489385/1‑76 (MQ=255) tAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATcc < 1:5298274/76‑1 (MQ=255) tAATGTCGATGATAGTGCGGCAGGGTTCTTCTTTCAGCTCGCCCAAATCGCGATCCAATTCACGCATCTGTAATcc > 1:4006008/1‑76 (MQ=255) aaTGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATcca < 1:4496746/76‑2 (MQ=255) aaTGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCg < 1:4526499/76‑1 (MQ=255) aaTGTCGATGATAGTGCGGCAGGGTTTTTCTTTTAGCTCGCCCAACCCCCGGTTCAATTCACCCCTCTGTAATccc > 1:761865/1‑75 (MQ=255) aaTGTCGATGATAGTGCGGCAGGGTTCTTTTTTTAGCTCGCCAAAATCGCGATCCAATTCACGCATCTGTAATCCg > 2:6336221/1‑76 (MQ=255) aaTGTCGATGATAGTGCGGCAGGGTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTTACCCCTCCTTAATCCg > 1:4937294/1‑76 (MQ=255) aTGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGt < 2:192265/76‑1 (MQ=255) aTGTCGATGATAGGGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGt < 2:2757160/76‑1 (MQ=255) tGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTa < 1:3013396/76‑1 (MQ=255) tGTCGATGATAGTGCGGCAGGGTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTTACGCATCTGTAATCCCTa > 2:3926080/1‑76 (MQ=255) gTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTaa < 2:2333067/76‑1 (MQ=255) gTCGATGATAGTGCGGCAGGGTTTTTCTTTTAGCTCGCCAAACTCGCGGTCCAAATCACGCATCTGTAATCCGTaa > 2:4233397/1‑76 (MQ=255) tCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAAc < 2:2913467/76‑1 (MQ=255) cGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAAcc > 1:6061701/1‑76 (MQ=255) cGATGATAGTGCGGCAGGGTTTTTCTTTCAGCTCGCCCAACTCGCGATCCAATTCACGCATCTGTAATCCGTAAcc > 2:1601240/1‑76 (MQ=255) cGATGATAGTGCGGCAGGGTTCTTCTTTTAGCTCGCCCAACTCGCGATCCAATTCACGCATCTGTAATCCGTAAcc > 2:6505569/1‑76 (MQ=255) cGATGATAGTGCGGCAGGGTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTTACGCATCTGTAATCCGTAAcc > 2:6254909/1‑76 (MQ=255) gatgatAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCa < 2:4108335/76‑1 (MQ=255) gatgatAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCa < 1:6691227/76‑1 (MQ=255) ttatAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACg < 2:1031642/74‑1 (MQ=255) tgatATTGCTGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACg < 2:7833777/76‑1 (MQ=255) tgatAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACg < 2:5546625/76‑1 (MQ=255) gatAGTGCGGCAGGTTTTTTCTTTCAGCTCGCCAAACTCCCGATCCAATTTACGCATCTGTAATCCGTAACCACGc > 2:7182539/1‑76 (MQ=255) atAGTGCGGCAGGGTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCCTAACCCCGCt > 2:5486088/1‑76 (MQ=255) gCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCaata < 2:8120796/76‑1 (MQ=255) gCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAAtt < 2:2078131/76‑1 (MQ=255) gCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAAtt < 1:3865686/76‑1 (MQ=255) gCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAAtt < 1:1841377/76‑1 (MQ=255) cAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTg < 1:1130635/76‑1 (MQ=255) tttGTTCTTTCAGGTCGTCAAACGCGCGGTCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTg < 1:8104674/76‑1 (MQ=255) tttCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTg < 2:5665138/76‑1 (MQ=255) | GCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTG > REL606/2355939‑2356089 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |