| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,169,225 | 0 | T | G | 36.6% | 408.0 / 21.8 | 205 | intergenic (+41/‑172) | rpoC/htrC | DNA‑directed RNA polymerase subunit beta'/heat shock protein |
| Reads supporting (aligned to +/- strand): ref base T (28/102); new base G (75/0); total (103/102) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.83e-33 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 230 total aligned reads displayed.
TGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATG > REL606/4169150‑4169300 | aCGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGtttt < 1:6981462/76‑1 (MQ=255) gCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGtttttt < 2:4818473/76‑1 (MQ=255) cAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGttttttt < 2:3865411/76‑1 (MQ=255) cAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGttttttt < 1:5912743/76‑1 (MQ=255) cAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGttttttt > 2:239671/1‑76 (MQ=255) cAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGgtttttt > 1:3405746/1‑76 (MQ=255) ggTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGgtttttttt > 2:7805586/1‑74 (MQ=255) ggTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTAt < 1:4903502/76‑1 (MQ=255) gTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATg < 2:39060/76‑1 (MQ=255) tCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATgg < 1:3312178/76‑1 (MQ=255) cTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGGTTTTTTTTggg > 1:7752715/1‑76 (MQ=255) tGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATgggg > 1:5966932/1‑76 (MQ=255) tGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATgggg < 2:4165083/76‑1 (MQ=255) tGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGGTTTTTTTTgggg > 1:5762723/1‑76 (MQ=255) gggCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGGTTTTTTTTggggg > 2:7183021/1‑76 (MQ=255) gggCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGGTTTTTTATtgggg > 2:2101394/1‑76 (MQ=255) ggCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATgggggg < 2:6390155/76‑1 (MQ=255) gCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATggggggg > 2:7988066/1‑75 (MQ=255) gCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGa < 1:1046405/76‑1 (MQ=255) gCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGa < 2:2973874/76‑1 (MQ=255) gCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGa < 1:7307093/76‑1 (MQ=255) gCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGGTTTTTTTTggggggg > 2:2672340/1‑75 (MQ=255) cGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGGTTTTTTTTgggggggg > 2:1178607/1‑74 (MQ=38) ggTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGt < 1:718211/76‑1 (MQ=255) ggTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGGTTTTTTTTggggggggg > 1:7722054/1‑73 (MQ=38) ttCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGttt < 2:5384329/76‑1 (MQ=255) ttCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGGTTTTTTATGGGGGGAGgtt > 1:1748954/1‑76 (MQ=255) cTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAg < 2:1935152/76‑1 (MQ=255) tGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGGGGTTAgg > 2:3785718/1‑76 (MQ=255) tGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAgg < 2:761037/76‑1 (MQ=255) gATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAggg < 1:6576470/76‑1 (MQ=255) aTAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGa < 2:4667826/76‑1 (MQ=255) aTAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGa < 1:5558642/76‑1 (MQ=255) tAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGGTTTTTTTTGGGGGGGGTTTTGGGaa > 1:3948300/1‑76 (MQ=38) aCGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAag < 2:3663249/76‑1 (MQ=255) aCGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAag < 2:1547020/76‑1 (MQ=255) aCGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAag < 1:7453397/76‑1 (MQ=255) cgcgTAATCGTTAATCCCTAAATAACGTTCAGGCCCGCGTCGGCTGGTTTGTTTATAAGGAGAGTATAGGGAAaga < 2:4986788/73‑1 (MQ=255) cGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAaga < 1:8002165/76‑1 (MQ=255) gAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGGTTTTTTTTGGGGGGGGGTTAGGGGAagag > 1:1387955/1‑76 (MQ=37) aGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGGTTTTTTTTGGGGGGAGGTTAGGGGAAGAGc > 2:2957530/1‑76 (MQ=38) gTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGGTTTTTTATGGGGGGGGGTTAGGGGAaaagac > 2:617636/1‑74 (MQ=37) tAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGGGTTTAGGGAAAGAGCAt > 2:5962331/1‑76 (MQ=255) tAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGGTTTTTTATGGGGGGAGGTTAGGGAAAGAGCAt > 2:7052438/1‑76 (MQ=255) aTCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCAttt < 2:6208817/76‑1 (MQ=255) aTCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCAttt < 1:628602/76‑1 (MQ=255) aTCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGGTTTTTT‑TGGGGGGGGTTTAGGGAAAAAGCATTTg > 2:183746/1‑76 (MQ=37) cGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGt < 2:4868019/76‑1 (MQ=255) cGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGGTTTTTTTTGGGGGGGGGTTTGGGGAAGAGGATTTGt > 1:2685105/1‑76 (MQ=25) ttAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCa < 2:3763901/76‑1 (MQ=255) ttAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCa > 1:1665641/1‑76 (MQ=255) ttAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGGTTTTTTTTGGGGGGGGGTTTGGGAAAGAGCATTTGTCa > 1:5078123/1‑76 (MQ=37) tAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAg < 1:8146807/76‑1 (MQ=255) tAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGGTTTTTTTTGGGGGGGGGTTAGGGAAAGAGCATTTTTCAg > 2:3395858/1‑76 (MQ=37) aaTCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGa < 2:5464406/76‑1 (MQ=255) aaTCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGa < 1:5418549/76‑1 (MQ=255) aTCCGCAAATAACGTAAAAACCCGCTTCGGCGGGGTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGaa > 2:5743099/1‑76 (MQ=255) tCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAat < 2:2495080/76‑1 (MQ=255) tCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAat < 1:1419480/76‑1 (MQ=255) ccGCAAATAACGTAAAAACCCGCTTCGGCGGGGTTTTTTTTGGGGGGGGGTTTGGGGAAGAGCATTTGTCAGAata > 1:927784/1‑76 (MQ=25) cGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAatat < 1:5463675/76‑1 (MQ=255) gCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATAtt < 1:7856084/76‑1 (MQ=255) gCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATAtt < 2:500389/76‑1 (MQ=255) aaaTAACGTAAAAACCCGCTTCGGCGGGGTTTTTTATGGGGGGAGGTTTGGGAAAGAGCATTTGTCAGAATATTTa > 2:6741089/1‑76 (MQ=38) aaTAACGTAAAAACCCGCTTCGGCGGGGTTTTTTTTGGGGGGGGGTTTGGGAAAGAGCATTTGTCAGAATAtttta > 1:946680/1‑74 (MQ=37) aTAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAg < 2:1569998/76‑1 (MQ=255) aTAACGTAAAAACCCGCTTCGGCGGGGTTTTTTTTGGGGGGGGGTTTGGGGAAGAGCATTTGTCAAAATATTTAAg > 1:2774657/1‑76 (MQ=25) tAACGTAAAAACCCGCTTCGGCGGGGTTTTTTTTGGGGGGGGGTTAGGGAAAGAGCATTTGTCAGAATATTTaaag > 2:6698021/1‑74 (MQ=37) aaCGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGa < 1:2437560/76‑1 (MQ=255) aaCGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGa < 1:4620880/76‑1 (MQ=255) aaCGTAAAAACCCGCTTCGGCGGGGTTTTTTTTGGGGGGGGGTTTGGGAAAGAGGATTTGTCATAATAATTTAGGa > 2:6431832/1‑76 (MQ=21) aaCGTAAAAACCCGCTTCGGCGGGGTTTTTTTTGGGGGGAGTTTAGGGAAAAAGCATTTGTCAGAATATTTAAgaa > 2:4562329/1‑74 (MQ=38) aaCGTAAAAACCCGCTTCGGCGGGGTTTTTTTTGGGGGGAGGTTAGGGAAAAAGCATTTGTCAGAATATTTAAAGa > 2:5026045/1‑76 (MQ=37) aCGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGGGTTTAGGGAAAGAGCCTTTGTCAGAATATTTAAGGaa > 2:7676992/1‑76 (MQ=255) cGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAAt < 1:5359095/76‑1 (MQ=255) gTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAAtt < 1:5604744/76‑1 (MQ=255) gTAAAAACCCGCTTCGGCGGGGTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAAtt > 2:967245/1‑76 (MQ=255) tAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAAttt > 2:1742665/1‑76 (MQ=255) tAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGGTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAAttt > 2:3145909/1‑76 (MQ=255) tAAAAACCCGCTTCGGCGGGGTTTTTTATGGGGGGGGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAAGAAttt > 2:5343681/1‑76 (MQ=38) tAAAAACCCGCTTCGGCGGGGTTTTTTATGGGGGGGGGTTAGGGGAAGAGCATTTGTCAGAATATTTAAGGAAttt > 2:487589/1‑76 (MQ=38) aaaaCCCGCTTCGGCGGGGTTTTTTTTGGGGGGGGTTTAGGGAAAGAGAAATTGTCAGAATATTTAAGGAATTTCt > 1:6054888/1‑76 (MQ=38) aaaCCCGCTTCGGCGGGGTTTTTTATGGGGGGGGTTTAGGGAAAGAGCATTTGTCAGAAAATTTAAGGAATTTCTg > 1:2017707/1‑76 (MQ=38) aaCCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGa > 2:8096048/1‑76 (MQ=255) aaCCCGCTTCGGCGGGGTTTTTTTTGGGGGGGGTTTAGGGAAAGAGCATTTGTCAGAATATTTTAGGAAATTCTGa > 2:1312549/1‑76 (MQ=38) aaCCCGCTTCGGCGGGGTTTTTTATGGGGGGGGGTTTGGGGAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGa > 2:7497266/1‑76 (MQ=38) cccGCTTCGGCGGGGTTTTTTATGGGGGGGGTTTTGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAAt > 2:7136900/1‑76 (MQ=39) cccGCTTCGGCGGGGTTTTTTATGGGGGGGGGTTAGGGAAAGAGCCTTTGTCAGAATATTTAAGGAATTTCTGAAt > 2:3600850/1‑76 (MQ=38) ccGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATa < 1:4025279/76‑1 (MQ=255) gCTTCGGCGGGGTTTTTTTTTGGGGGGGGTTAGGGGAAGAGCATTTGTCAGAATATTTAAGGAATTTCTTAATACt > 1:4945209/1‑76 (MQ=37) ttCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCa < 1:3581162/76‑1 (MQ=255) tCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCAt < 1:6336647/76‑1 (MQ=255) tCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCAt < 1:6436789/76‑1 (MQ=255) tCGGCGGGGTTTTTTTTGGGGGGGGTTTAGGGGAAGAGAATTTGTTAGAATATTTAAGGAATTTCTGAATACTCAt > 2:564418/1‑76 (MQ=255) ggcggGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATaa < 1:7475999/76‑1 (MQ=255) ggcggGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATaa > 2:5807187/1‑76 (MQ=255) gcggGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAAt < 1:720452/76‑1 (MQ=255) cggGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATc < 1:3761196/76‑1 (MQ=255) tttttttATGGGGGGAGTTTAGGGAAAGAGCATTTGTCTGAATATTTAAGGAATTTCTGAATACTCATAATCAATg < 1:4189641/76‑1 (MQ=255) tttttttATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATg < 1:1524257/76‑1 (MQ=255) | TGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATG > REL606/4169150‑4169300 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |