| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,118,209 | 0 | G | T | 35.5% | 64.3 / 49.4 | 62 | intergenic (‑444/‑311) | csgD/csgB | DNA‑binding transcriptional activator in two‑component regulatory system/curlin nucleator protein, minor subunit in curli complex |
| Reads supporting (aligned to +/- strand): ref base G (6/34); new base T (22/0); total (28/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.10e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.91e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 155 total aligned reads displayed.
TAAAACGTTCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTC‑T‑GGTGCTTTTTGATAGCGGAAAACG > REL606/1118134‑1118284 | tAAAACGTTCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTtg < 2:2746133/76‑1 (MQ=255) aaaCGTTCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTtgtg < 1:2365103/76‑1 (MQ=255) aaCGTTCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTgc < 1:3486802/76‑1 (MQ=255) gTTCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCa < 2:7583097/76‑1 (MQ=255) gTTCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCa < 1:2063253/76‑1 (MQ=255) ttCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCaa < 1:134812/76‑1 (MQ=255) tCTTGTTTTGTCTCCGCACCTCCGTGGAGAATTTTTTGCTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAg < 2:4932681/76‑1 (MQ=255) gTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACat < 2:3914039/76‑1 (MQ=255) gTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACat < 1:3124886/76‑1 (MQ=255) gTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACat < 1:1725886/76‑1 (MQ=255) ttttttCTCCACCCCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGTTTTGTCACGGCTTGTGCGCAAGACata < 2:323872/76‑1 (MQ=255) ttttttCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACata < 2:3613555/76‑1 (MQ=255) ttttttCCCCACCCCTCCGTGAACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACata < 1:7433341/76‑1 (MQ=255) ttttCTCCACCCCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGTTTGTGCGAAAGACATATc < 2:1958812/76‑1 (MQ=255) ttCTCCACCCCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCgc < 2:5236947/76‑1 (MQ=255) ttCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTGTGCCAGACATATCgc > 1:3665508/1‑76 (MQ=255) ttCTCAACACCTCCGTGAACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCgc < 2:1942321/76‑1 (MQ=255) ctcCACCCCTCCGTGGACAATTTTTTACTGCAAAAAGACGGGTTTTGTCACGCTTTGTGCGCAAGACATATCgcag < 2:5292838/76‑1 (MQ=255) ctcCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTTCGCAAAGCAAATCgcag > 1:3903469/1‑76 (MQ=255) ctcCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTGGGCAAGACATATTgccg > 1:144448/1‑74 (MQ=255) tcCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTTGGGCAGGCATATTgcagc > 1:2702509/1‑76 (MQ=255) tcCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTTCGCGAGACAGATCgcaac > 2:3745088/1‑74 (MQ=255) tcCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGAGCAAGACATATCgcagc < 1:4736758/76‑1 (MQ=255) ccACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTGTGCCAGACATATCgcagca > 2:2313655/1‑76 (MQ=255) ccACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTGTGCACGACATAAAgcagca > 2:3926196/1‑76 (MQ=255) acacCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTGCGCAAGACATATCgcagccaa > 2:6320194/1‑73 (MQ=255) ccTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAg < 1:246922/76‑1 (MQ=255) cTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTTGGCGAGGCATAACGCCGCAAACAGc > 1:2882015/1‑76 (MQ=255) tCCGTGGACATTTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGAAAGACATATCGCAGCAATCAGCg < 2:4305354/76‑1 (MQ=255) cggTGGACATTTTTTTACTAAAAAAAGACGAGGTTTGTCACGGCTTGTGCGAAAGACATATCGCAGCAATCAGCGa < 2:648023/74‑1 (MQ=255) cggTGAACATTTTTTTACTGAAAAAAGACGAGGTTTGCCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGa < 2:7763213/74‑1 (MQ=255) ccGTGGACATTTTTTTACTGCAAAAAGACGGGGTTTGCCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGa < 2:2007768/76‑1 (MQ=255) ccGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTTGGCAAGACATATCCCAGCCAACACCGa > 2:7515120/1‑76 (MQ=255) gTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTGTGCAAGACATATCGCAGCCATAAACGaag > 2:4989815/1‑74 (MQ=255) gACAATTTTTTACTGAAAAAAGACGAGGTTTGTCCCGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGc < 1:6594875/76‑1 (MQ=255) aCAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTGCGCAAGACATATCGCAGCAATCAGCGACGGGCa > 2:8118163/1‑76 (MQ=255) aCAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCa < 2:2239736/76‑1 (MQ=255) aCAATTTTTTACTGAAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCa < 1:6288611/76‑1 (MQ=255) aCAATTTTTTACTGAAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCa < 2:5987514/76‑1 (MQ=255) aCAATTTTTTACGGAAAAAAGACGAGGTTTGTCCGGGCTTGTGCGAAAGACATATCGCAGCAATCAGCGACGGGCa < 2:1246130/76‑1 (MQ=255) aaaTTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGAAAGACATATCGCAGCAATCAGCGACGGGCaa < 2:4871230/75‑1 (MQ=255) aaaTTTTTTACTGAAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCaa < 1:2077192/75‑1 (MQ=255) aaTTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTGCGCAAGAAATATCGCAGCAATCAGCGGAGGGCcag > 1:8160115/1‑76 (MQ=255) aaTTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGAAAGACATATCGCAGCAATCAGCGACGGGCaag < 2:1067245/76‑1 (MQ=255) aTTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTTCGCCAGACAAATCGCCACAATCAGCGACGGGCgaga > 2:1323822/1‑76 (MQ=255) aTTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCaaga < 2:6092122/76‑1 (MQ=255) ttttttACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGAAAGACATATCGCAGCAATCAGCGACGGGCaagaa < 1:7396907/76‑1 (MQ=255) ttttttACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGAAAGACATATCGCAGCAATCAGCGACGGGCaagaa < 2:2552309/76‑1 (MQ=255) ttttttACTGAAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCaagaa < 1:2982440/76‑1 (MQ=255) tttttACTGCAAAAAGACGAGGTTTGTCACGGCTTTTGGGCGAGACATATCGCCGCAAACAGCGGCGGGCgagaaa > 1:2853286/1‑75 (MQ=255) tttttACTGCAAAAAAAAGAGTTTTGTCACGGCTTGAGCGCAAGACATATCGCAGCAATCAGCGACGGGCaagaag < 2:7232468/76‑1 (MQ=255) tttttACTGAAAAAAGACGAGGTTTGTCACGGCTGGTGCAAAAGACATATCGCAGCAATCAGCGACGGGCaagaag < 2:4247734/76‑1 (MQ=255) tttttACTAAAAAAAGGCGGGGTTTGTCACGGTTTGTGCGAAAGACATATCGCAGCAATCAGCGACGGGCaagaag < 2:671468/76‑1 (MQ=255) ttttACTAAAAAAAGACGGGTTTTGTCACGGCTTGTGCGAAAGACATATCGCAGCAATCAGCGACGGGCAagaaga < 2:5506695/76‑1 (MQ=255) tttACTGCAAAAAGACGAGGTTTGTCACGGCTTTTTCGCCAGACATAACGCAGCAATCAGCGACGGGGCAgaagaa > 2:4822587/1‑76 (MQ=255) ttACTGCAAAAAGACGAGGTTTGTCACGGCTTTTTTGGCAGACATATCGCAGCCAACAGCGCCGGGCAAaaagaaa > 1:4401524/1‑75 (MQ=255) ttACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAAt < 2:2181228/76‑1 (MQ=255) ttACTGAAAAAAGACGGGGTTTGTCACGGTTGGGGCACAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAAt < 2:7390494/76‑1 (MQ=255) ttACTGAAAAAACGCGAGTTTTGCCGCGGCTTGTCCGAAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAAt < 1:2899817/76‑1 (MQ=255) tACTGCAAAAAGACGAGTTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATg < 2:6228585/76‑1 (MQ=255) tACTGAAAAAAGACGAGGTTTGCCCGGCCTTGCCCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATg < 1:89662/76‑1 (MQ=255) aCTGCAAAAAGACGAGGTTTGCCACGGCTTGTGCAAAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGa < 1:3645949/76‑1 (MQ=255) aCTGAAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGa < 2:7893250/76‑1 (MQ=255) cTGCAAAAAGACGAGGTTTGTCACGGCTTTTTCGCAAGACATATCGCAGCAAACAACGACGGGGAAGGAGAAAGaa > 1:4082796/1‑75 (MQ=255) cTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGAc < 2:7709578/76‑1 (MQ=255) tGCAAAAAGACGAGGTTTGTCACGGCTTTTTGGCCAGAAAAATCGGAGGCAACAGCGACGGGGAAGAAAAAAGaat > 2:4699666/1‑74 (MQ=255) cAAAAAGACGAGGTTTGTCACGGCTTTTGCGGAAGAAATATCCCAGCAATCAGCGACGGGGCAGGAGAAAGAATGt > 2:5446671/1‑76 (MQ=255) aaaaaGACGAGGTTTGTCACGGCTTTTGCGCGAGACATATCGCCGCAAACAGCGACGGGGAAAAAGAAAGAATGTc > 1:5124063/1‑76 (MQ=255) aaaaGACGAGGTTTGTCACGGTTTGTGGCAAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTC‑t < 2:4193338/76‑1 (MQ=255) aaaGACGAGGTTTGTCACGGCTTTTGCGCAAGACAAATACCAGCAATCAGCGACGGGGGAGGAGGAAGACTGTC‑T‑g > 2:1459648/1‑76 (MQ=255) aaGACGAGGTTTGTCACGGCTTTTGCGCCAAACAAATTTCAGCACACAGCGACGGGCCAGAAAAAAAAATGTC‑ttg > 2:2453399/1‑74 (MQ=255) aaGACGAGGTTTGTCACGGCTTGTGCGAAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTC‑T‑gg < 1:7745152/76‑1 (MQ=255) aCGAGGTTTGTCACGGCTTTTGCGCAAGACATATCGCAGCAATCAGCGACGGGCCAGAAGAATGAATGTC‑T‑ggggg > 2:666790/1‑73 (MQ=255) cGAGGTTTGGCACGGCTTGTGCGAAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTC‑T‑GGTGCt < 2:4402457/76‑1 (MQ=255) gggTTTGTCACGGCTGGTGCACAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTC‑T‑GGTGCttt < 1:6623634/75‑1 (MQ=255) tttGTCACGGCTTGTGCACAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTC‑T‑GGTGCTTTTTg < 2:5884525/76‑1 (MQ=255) ttGTCACGGCTTTTGGGCAAGAAATATCGCAGCAATAAGCGACGGGGGAGAAAAAAGACTGTC‑TGGGGGCtttttt > 2:917672/1‑75 (MQ=255) tGTCGCGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTC‑T‑GGTGCTTTTTGAt < 2:5714888/76‑1 (MQ=255) tGTCACGTCTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTC‑T‑GGTGCTTTTTGAt < 2:320262/76‑1 (MQ=255) tGTCACGGCTTTTGTGCCAGACATATCGCAGCAATCAGCGACGGGGCAGAAAAAAGAATGTCTT‑GGGGCttttttt > 2:1003904/1‑74 (MQ=255) gTCACGGTTTGTGCAAAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTC‑T‑GGTGCTTTTTGATa < 1:1136215/76‑1 (MQ=255) cACGGCGGGTGCGAAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTC‑T‑GGTGCTTTTTGATAGc < 1:7985451/76‑1 (MQ=255) aCGGCTGGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTC‑T‑GGTGCTTTTTGATAGCg < 2:1296058/76‑1 (MQ=255) cGGCTTGTGCGAAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTC‑T‑GGTGCTTTTTGATAGCgg < 1:6443954/76‑1 (MQ=255) ggCTTGTGCGAAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTC‑T‑GGTGCTTTTTGATAGCGGa < 1:1756194/76‑1 (MQ=255) ggCTGGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTC‑T‑GGTGCTTTTTGATAGCGGa < 2:2278795/76‑1 (MQ=255) gCTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTC‑T‑GGTGCTTTTTGATAGCGGaa < 2:8134474/76‑1 (MQ=255) gCTTGTGCGAAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTC‑T‑GGTGCTTTTTGATAGCGGaa < 2:6694186/76‑1 (MQ=255) cTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTC‑T‑GGTGCTTTTTGATAGCGGaaa > 1:7251499/1‑76 (MQ=255) cTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTC‑T‑GGTGCTTTTTGATAGCGGaaa > 1:4007696/1‑76 (MQ=255) cTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTC‑T‑GGTGCTTTTTGATAGCGGaaa > 1:6962349/1‑76 (MQ=255) cTTGTGCGAAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTC‑T‑GGTGCTTTTTGATAGCGGaaa < 1:1589691/76‑1 (MQ=255) ttGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTC‑T‑GGTGCTTTTTGATAGCGGaaaa < 2:6064758/76‑1 (MQ=255) ttGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTC‑T‑GGTGCTTTTTGATAGCGGaaaa > 2:591637/1‑76 (MQ=255) ttGTGCGAAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTC‑T‑GGTGCTTTTTGATAGCGGaaaa < 1:5196873/76‑1 (MQ=255) gtgGGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTC‑T‑GGTGCTTTTTGATAGCGGAAAACg > 2:6863941/1‑76 (MQ=255) gtgCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTC‑T‑GGTGCTTTTTGATAGCGGAAAACg > 1:7563886/1‑76 (MQ=255) gtgCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTC‑T‑GGTGCTTTTTGATAGCGGAAAACg > 2:5780429/1‑76 (MQ=255) gtgCGAAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTC‑T‑GGTGCTTTTTGATAGCGGAAAACg < 2:3566658/76‑1 (MQ=255) gtgCGAAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTC‑T‑GGTGCTTTTTGATAGCGGAAAACg < 2:647914/76‑1 (MQ=255) | TAAAACGTTCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTC‑T‑GGTGCTTTTTGATAGCGGAAAACG > REL606/1118134‑1118284 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |