| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 649,731 | C→A | W357C (TGG→TGT) | mrdA ← | transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 649,731 | 0 | C | A | 98.8% | 691.6 / ‑4.0 | 171 | W357C (TGG→TGT) | mrdA | transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2) |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base A (87/82); total (87/84) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 183 total aligned reads displayed.
TTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGT > REL606/649658‑649803 | ttGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGccaaca < 1:4082076/76‑1 (MQ=255)ttGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGccaaca > 2:7110256/1‑76 (MQ=255) tgtgACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAg < 1:5590563/76‑1 (MQ=255) tgtgACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAg < 1:1806122/76‑1 (MQ=255) gtgACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGc < 1:6132551/76‑1 (MQ=255) tgACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGcc > 1:5845692/1‑76 (MQ=255) tgACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGcc < 1:2122850/76‑1 (MQ=255) tgACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGcc < 2:486122/76‑1 (MQ=255) aCATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTg < 2:6224486/76‑1 (MQ=255) cATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTgg < 2:801707/76‑1 (MQ=255) aTTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTggg < 2:6251772/76‑1 (MQ=255) ttCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTggat > 1:2458099/1‑74 (MQ=255) ttCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGt < 1:4478952/76‑1 (MQ=255) ttCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGt < 2:531971/76‑1 (MQ=255) tCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTc < 2:1296356/76‑1 (MQ=255) tCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTc < 1:2104767/76‑1 (MQ=255) gACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCaaa < 2:2901668/76‑1 (MQ=255) aCGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAAc < 1:6213969/76‑1 (MQ=255) gCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAg > 2:4571150/1‑76 (MQ=255) gCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAg < 2:903324/76‑1 (MQ=255) cccGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGc > 1:5294841/1‑76 (MQ=255) cccGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGc < 1:1092467/76‑1 (MQ=255) ccGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGcg < 2:1958210/76‑1 (MQ=255) ccGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGcg > 2:1483359/1‑76 (MQ=255) ccGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGcg < 1:55389/76‑1 (MQ=255) ccGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGcg < 2:7680640/76‑1 (MQ=255) gTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGcgtc < 2:363012/76‑1 (MQ=255) ggCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGcgtcgt > 1:7238811/1‑76 (MQ=255) cccATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTAtt < 1:4360988/76‑1 (MQ=255) cccATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTAtt < 1:4014129/76‑1 (MQ=255) ccATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTg > 1:3976976/1‑76 (MQ=255) ccATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTg < 2:4366473/76‑1 (MQ=255) cATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTgc < 2:977711/76‑1 (MQ=255) aTTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTgcg < 1:4236192/76‑1 (MQ=255) aTTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTgcg > 2:1583680/1‑76 (MQ=255) tttttCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGcgcg < 1:11629/76‑1 (MQ=255) ttttCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGt > 1:3968325/1‑76 (MQ=255) ttttCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGt > 1:3014946/1‑76 (MQ=255) ttttCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGt > 1:7891184/1‑76 (MQ=255) tttCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTg > 1:7408021/1‑76 (MQ=255) ttCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGa > 2:6887519/1‑76 (MQ=255) ttCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGa > 2:7479153/1‑76 (MQ=255) tCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGAt > 1:2836425/1‑76 (MQ=255) tCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGAt > 1:3364668/1‑76 (MQ=255) ccAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATc > 1:664232/1‑76 (MQ=255) ccAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATc > 1:3836082/1‑76 (MQ=255) ccAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATc > 1:995802/1‑76 (MQ=255) cAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCa < 2:8144483/76‑1 (MQ=255) tCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCAccc > 2:7196491/1‑76 (MQ=255) tCACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACACCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCAccc < 1:6307402/76‑1 (MQ=255) cACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCAcccc < 2:1372877/76‑1 (MQ=255) cACGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGACTGGGCCAAACAGCGTCGTATTGCGCGTGATCAcccc > 1:6121588/1‑76 (MQ=255) aCGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCg < 1:812758/76‑1 (MQ=255) cGATAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTAATCACCCCgg > 2:8012484/1‑76 (MQ=255) tAACGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGgcgc > 1:3177874/1‑76 (MQ=255) aaCGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCt > 1:371003/1‑76 (MQ=255) aaCGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCt > 1:3618363/1‑76 (MQ=255) aaCGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCt > 1:4706571/1‑76 (MQ=255) aaCGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCt > 1:7501460/1‑76 (MQ=255) aaCGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCt > 1:4493836/1‑76 (MQ=255) aCGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTc < 1:3551079/76‑1 (MQ=255) cGTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCa < 2:3307265/76‑1 (MQ=255) gTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCaa > 1:7062253/1‑76 (MQ=255) gTTTTTCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCaa > 2:6775709/1‑76 (MQ=255) tttttCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAAt < 2:7741221/76‑1 (MQ=255) tttttCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCGCAAt > 2:2327868/1‑76 (MQ=255) ttttCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATg < 2:5763732/76‑1 (MQ=255) tttCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGc < 1:483544/76‑1 (MQ=255) tttCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGc > 2:3245063/1‑76 (MQ=255) ttCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGcc > 1:7958301/1‑76 (MQ=255) ttCCGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGcc > 1:7060625/1‑76 (MQ=255) ccGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGa > 2:6350661/1‑76 (MQ=255) ccGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGa < 1:6918376/76‑1 (MQ=255) ccGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGa > 1:1706591/1‑76 (MQ=255) cGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGaa > 2:6361484/1‑76 (MQ=255) cGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGaa < 2:3762837/76‑1 (MQ=255) cGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGaa < 2:7566304/76‑1 (MQ=255) cGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGaa < 2:7914132/76‑1 (MQ=255) cGAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGaa < 2:5210619/76‑1 (MQ=255) gAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGaaa > 2:5884713/1‑76 (MQ=255) gAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGaaa > 1:7209054/1‑76 (MQ=255) gAACCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGaaa > 2:4163023/1‑76 (MQ=255) aaCCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAAc > 1:2182088/1‑76 (MQ=255) aCCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAAcc < 1:5979820/76‑1 (MQ=255) aCCTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAAcc < 2:1619183/76‑1 (MQ=255) ccTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCg > 1:7632775/1‑76 (MQ=255) ccTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCg < 2:7836461/76‑1 (MQ=255) cTGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGc > 1:6621592/1‑76 (MQ=255) cTGGCAGTTGCCAACAGCCTGGGGCAAACAGCGTCGTATTGCGCGTGATGACCCCGGCGCTCAATGCCGAAACCGc > 2:5717870/1‑76 (MQ=255) tGGCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGcc > 2:8068741/1‑76 (MQ=255) ggCAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCca > 2:5679570/1‑76 (MQ=255) cAGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCcaca > 1:464233/1‑76 (MQ=255) aGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACAt > 2:3588568/1‑76 (MQ=255) aGTTGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACAt > 2:439327/1‑76 (MQ=255) aGTGGCCAACAGCCGGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACAt < 1:7028906/76‑1 (MQ=255) ttGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAg > 1:890263/1‑76 (MQ=255) tGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACTGCCACATAgg > 2:2069983/1‑76 (MQ=255) tGCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAgg > 1:7673162/1‑76 (MQ=255) gCCAACAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAggg > 1:173291/1‑76 (MQ=255) ccaacaGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGt < 1:941715/76‑1 (MQ=255) | TTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGT > REL606/649658‑649803 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |