| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,338,855 | G→A | G229R (GGA→AGA) | yhdG → | tRNA‑dihydrouridine synthase B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,338,855 | 0 | G | A | 98.4% | 955.2 / 3.9 | 251 | G229R (GGA→AGA) | yhdG | tRNA‑dihydrouridine synthase B |
| Reads supporting (aligned to +/- strand): ref base G (3/1); new base A (129/117); total (133/118) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.04e-01 | |||||||||||
Only 100 of 276 total aligned reads displayed.
TGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGC > REL606/3338782‑3338928 | tGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAgaga < 1:3126784/76‑1 (MQ=255) gACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAACTCAGAGaa > 2:6694745/1‑76 (MQ=255) aCCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAg < 1:3776757/76‑1 (MQ=255) ccGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGAc < 2:8087837/76‑1 (MQ=255) gCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGAccc > 2:4769971/1‑76 (MQ=255) cTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCt > 2:42663/1‑76 (MQ=255) cTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCt > 1:4663788/1‑76 (MQ=255) ttAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTg < 2:435295/76‑1 (MQ=255) ttAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTg > 1:2721138/1‑76 (MQ=255) tAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTgg > 2:539410/1‑76 (MQ=255) aaaGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGa > 2:3028160/1‑76 (MQ=255) aaGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGAt < 2:7191742/76‑1 (MQ=255) cAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCttt < 1:2464169/76‑1 (MQ=255) cAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCttt < 1:904252/76‑1 (MQ=255) cAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGACGCGCTGCTCAGAGAAGACCCTGGATCttt > 2:3684304/1‑76 (MQ=255) agagCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTc < 1:2627006/76‑1 (MQ=255) agagCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTc > 2:450158/1‑76 (MQ=255) gagCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCg < 2:7435517/76‑1 (MQ=255) agCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCgg < 1:1290743/76‑1 (MQ=255) gCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCggg < 2:4285003/76‑1 (MQ=255) tgtgCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGaa < 2:3615267/76‑1 (MQ=255) tgtgCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGaa < 2:2439903/76‑1 (MQ=255) tgtgCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGaa > 2:1341426/1‑76 (MQ=255) gtgCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGGTCTTTCGGGaaa > 2:4668250/1‑76 (MQ=255) gtgCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGaaa > 1:122353/1‑76 (MQ=255) gtgCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGaaa > 2:2657149/1‑76 (MQ=255) gtgCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTAGGGaaa > 1:3836039/1‑76 (MQ=255) tgCTCGACTATACAGGGGCGGAGGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAAt > 2:1168588/1‑76 (MQ=255) gCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATc > 1:1450315/1‑76 (MQ=255) cTCGACTATACAGTGGCGGATGCCCTGATGATAGGCCGCGCATCTCAGAGAAGACCCTGGATCTTGCGGGAAATcc < 2:3759816/76‑1 (MQ=255) cTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATcc < 2:3954710/76‑1 (MQ=255) cTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATcc < 2:4999612/76‑1 (MQ=255) cGACTATACAGGGGCGGATTCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAg > 1:8064215/1‑76 (MQ=255) cGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAg > 1:4348329/1‑76 (MQ=255) gACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGc > 2:6719567/1‑76 (MQ=255) gACGATACAGGGGGGGGTGCCCTGCTGATAGGACGAGCGGCTGTCAGCACACCCAGCCGCTTTCGGGAAATccgca > 2:6746034/1‑73 (MQ=255) aCTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCa > 1:5729815/1‑76 (MQ=255) cTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCAt < 2:2569979/76‑1 (MQ=255) tataCAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCAtt > 1:2016928/1‑76 (MQ=255) ataCAGGGGCGGATGCCCTGATGATAGGCCGCGCAGGTCAGAGAAGAACCTGGATCTTTCGGGAAATACAGCATTa > 2:6579267/1‑76 (MQ=255) ataCAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCCGCATTa > 2:1617322/1‑76 (MQ=255) ataCAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTa < 1:438152/76‑1 (MQ=255) ataCAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTa > 1:7893144/1‑76 (MQ=255) aCAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATc < 1:5466129/76‑1 (MQ=255) aCAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATc < 1:4546695/76‑1 (MQ=255) aCAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATc > 1:4962822/1‑76 (MQ=255) cAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCt > 2:4833339/1‑76 (MQ=255) aGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTg > 1:7055372/1‑76 (MQ=255) gggCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGa < 1:5885565/76‑1 (MQ=255) ggCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGac < 2:6984638/76‑1 (MQ=255) ggCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGac < 2:4153746/76‑1 (MQ=255) gCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGaca < 1:6531409/76‑1 (MQ=255) gCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGaca < 2:7500994/76‑1 (MQ=255) ggATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACt > 1:6963304/1‑76 (MQ=255) gATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTg < 2:3252043/76‑1 (MQ=255) aTGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTgg > 1:7996917/1‑76 (MQ=255) aTGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTgg > 2:6200828/1‑76 (MQ=255) aTGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTgg < 2:3865206/76‑1 (MQ=255) tGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTggg > 1:2530551/1‑76 (MQ=255) tGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTggg < 1:7518339/76‑1 (MQ=255) gCCCTGATGATAGGCCGCGCAGCTGAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTgggg < 2:2292187/76‑1 (MQ=255) gCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACGCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTgggg > 1:1351787/1‑76 (MQ=255) gCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTgggg < 1:7805/76‑1 (MQ=255) ccTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAg < 1:3277476/76‑1 (MQ=255) ccTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAg < 1:1501235/76‑1 (MQ=255) ccTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAg < 1:4783738/76‑1 (MQ=255) ccTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAg > 1:8054489/1‑76 (MQ=255) cTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGt > 2:5089150/1‑76 (MQ=255) tgatgaTAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGtt > 1:968486/1‑76 (MQ=255) tgatgaTAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGtt > 2:581325/1‑76 (MQ=255) tgatgaTAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGtt > 1:1094224/1‑76 (MQ=255) tgatgaTAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGtt < 1:3644895/76‑1 (MQ=255) gatgatAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTtg > 1:4082241/1‑76 (MQ=255) gatgatAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTtg < 1:4824286/76‑1 (MQ=255) gatgatAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTtg < 1:5263974/76‑1 (MQ=255) gatgatAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTtg < 2:527175/76‑1 (MQ=255) atgatAGGCCGCGCAGCTCAGAGAAGACGCTGGATCTTTCGGGAAATCCCGCATTATCTGGACACTGGGGAGTtgc > 2:360/1‑76 (MQ=255) atgatAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTtgc < 1:724895/76‑1 (MQ=255) tgatAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTtgct < 2:2759629/76‑1 (MQ=255) gatAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTtgctg > 1:1874334/1‑76 (MQ=255) tAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGcc > 1:6190330/1‑76 (MQ=255) tAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGcc > 1:1751032/1‑76 (MQ=255) tAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGcc > 1:6828189/1‑76 (MQ=255) ggCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGcccc > 1:2300140/1‑76 (MQ=255) gCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGccccc < 1:5967152/76‑1 (MQ=255) ccGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCg < 1:7370775/76‑1 (MQ=255) cgcgCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGc > 2:6342045/1‑76 (MQ=255) cgcgCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCACCGc > 2:1658660/1‑76 (MQ=255) gcgcAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCt < 1:3076865/76‑1 (MQ=255) gcAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGc > 2:1383833/1‑76 (MQ=255) aGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCt > 2:2909585/1‑76 (MQ=255) cTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCttt > 1:6696296/1‑76 (MQ=255) cTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCttt > 1:1000474/1‑76 (MQ=255) cTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCttt > 2:4618429/1‑76 (MQ=255) cTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCttt > 1:3159684/1‑76 (MQ=255) tCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTg > 2:4407076/1‑76 (MQ=255) tCAGAGAAAACCCTGGATCTTTCGTGAAATTCAGCATAATCTGGACACTGGGGAGATGCTACCCCCGCAACCttgg > 2:3375719/1‑74 (MQ=255) cAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTgg > 2:197597/1‑76 (MQ=255) cAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTgg > 2:6050047/1‑76 (MQ=255) cAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTgg > 2:7707235/1‑76 (MQ=255) | TGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGC > REL606/3338782‑3338928 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |