| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,163,133 | A→C | N760H (AAC→CAC) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,163,133 | 0 | A | C | 97.9% | 1005.6 / 4.6 | 233 | N760H (AAC→CAC) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base A (4/1); new base C (120/108); total (124/109) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
Only 100 of 236 total aligned reads displayed.
AGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGC > REL606/4163060‑4163206 | aGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCAc < 2:846561/76‑1 (MQ=255)aGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCAc > 2:3817007/1‑76 (MQ=255) gTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCAcc < 2:765796/76‑1 (MQ=255) ttAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCa > 1:2500829/1‑76 (MQ=255) ttAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCa > 1:546794/1‑76 (MQ=255) ttAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCa > 1:6781587/1‑76 (MQ=255) ttAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCa > 1:2069650/1‑76 (MQ=255) ttAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCa > 1:1291617/1‑76 (MQ=255) tAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAg > 1:163911/1‑76 (MQ=255) tAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAg < 1:3058280/76‑1 (MQ=255) tAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAg > 1:7122658/1‑76 (MQ=255) aaCGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGa > 2:2034417/1‑76 (MQ=255) aCGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGaa > 1:800569/1‑76 (MQ=255) cGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAac < 2:2442297/76‑1 (MQ=255) gAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAaca < 2:1619737/76‑1 (MQ=255) gAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAaca < 1:6867458/76‑1 (MQ=255) gAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAaca > 1:4456644/1‑76 (MQ=255) aaGACGCGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAacac < 2:6026984/76‑1 (MQ=255) aCGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTg < 1:7815189/76‑1 (MQ=255) aCGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTg > 2:1185261/1‑76 (MQ=255) aCGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTg > 1:7081074/1‑76 (MQ=255) cGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGt > 2:1662034/1‑76 (MQ=255) cGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGt < 2:7041310/76‑1 (MQ=255) agaTGTATGCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTAt < 2:6380826/76‑1 (MQ=255) agaTGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTAt < 1:1608994/76‑1 (MQ=255) gaTGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATc > 2:432894/1‑76 (MQ=255) gaTGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATc < 2:7740437/76‑1 (MQ=255) aTGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCa < 1:4609138/76‑1 (MQ=255) aTGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCa < 1:5177133/76‑1 (MQ=255) gTATCCGGGTGAAGCAGGTATCGACATCTCCAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAAc > 1:6646533/1‑76 (MQ=255) gTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAAc > 1:4020329/1‑76 (MQ=255) gTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAAc > 1:6747219/1‑76 (MQ=255) gTACCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAAc < 1:4931140/76‑1 (MQ=255) tATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAAcc > 1:6543574/1‑76 (MQ=255) aTCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCa < 1:2431667/76‑1 (MQ=255) aTCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCa < 1:4390161/76‑1 (MQ=255) tCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAg < 2:823940/76‑1 (MQ=255) ccGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGa < 1:3217141/76‑1 (MQ=255) ccGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGa < 1:5903386/76‑1 (MQ=255) cGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGAt > 2:684148/1‑76 (MQ=255) tgTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGc > 2:2857039/2‑76 (MQ=255) tGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCg < 1:1132418/76‑1 (MQ=255) gAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCgt < 2:3752440/76‑1 (MQ=255) aaGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCgtg < 2:1510034/76‑1 (MQ=255) cAGGTATCGACATCTACAACCTGACCAAATACTCCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCgtgtgt < 1:4740676/76‑1 (MQ=255) cAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCgtgtgt > 1:7953550/1‑76 (MQ=255) ggTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCgtgtgtgt < 2:6141784/76‑1 (MQ=255) ggTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCgtgtgtgt > 1:6095155/1‑76 (MQ=255) ggTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCgtgtgtgt > 1:5300951/1‑76 (MQ=255) tATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGtct < 2:5104767/76‑1 (MQ=255) tATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGtct > 1:4544830/1‑76 (MQ=255) tATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGtct < 2:6592290/76‑1 (MQ=255) aTCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGtctc > 1:1839237/1‑76 (MQ=255) aTCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGtctc > 2:3279998/1‑76 (MQ=255) tCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTctct > 1:7292852/1‑76 (MQ=255) tCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTctct > 2:5339621/1‑76 (MQ=255) cGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTg > 1:3512946/1‑76 (MQ=255) gACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTgg > 1:813202/1‑76 (MQ=255) cATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGt > 2:263378/1‑76 (MQ=255) aTCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTg > 1:4160290/1‑76 (MQ=255) aTCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTg > 2:5032134/1‑76 (MQ=255) tCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGa < 1:7220638/76‑1 (MQ=255) cTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGaa > 2:4521220/1‑76 (MQ=255) tACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAAc < 2:5260971/76‑1 (MQ=255) aCAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAAcc < 1:2809483/76‑1 (MQ=255) ccTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCGGATGCCGTGTGTGTCTCTGGGTGAACCGGtt < 1:3299544/76‑1 (MQ=255) ccTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGtt < 1:2161034/76‑1 (MQ=255) ccTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGtt > 1:3011195/1‑76 (MQ=255) cTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTg > 2:6726771/1‑76 (MQ=255) cTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTg < 2:2917643/76‑1 (MQ=255) cTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTCTGTGTCTCTGGGTGAACCGGTTg > 1:5970374/1‑76 (MQ=255) tGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGa > 2:3530902/1‑76 (MQ=255) gACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGaa > 1:6314234/1‑76 (MQ=255) gACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGaa < 2:4871750/76‑1 (MQ=255) gACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGCGAACCGGTTGaa > 2:6258502/1‑76 (MQ=255) aCCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAAc < 2:4412043/76‑1 (MQ=255) ccAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACg < 2:6747219/76‑1 (MQ=255) cAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGt > 1:6454560/1‑76 (MQ=255) cAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGt < 1:1505361/76‑1 (MQ=255) cAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGt > 1:2624902/1‑76 (MQ=255) cAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGt > 1:7392323/1‑76 (MQ=255) cAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGt > 1:1011705/1‑76 (MQ=255) aaaTACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTg > 1:5724443/1‑76 (MQ=255) aaaTACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTg > 1:3568647/1‑76 (MQ=255) aTACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGc < 2:1447300/76‑1 (MQ=255) aTACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGc > 2:2115051/1‑76 (MQ=255) tACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCg > 2:3478392/1‑76 (MQ=255) acacCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGa > 1:3412250/1‑76 (MQ=255) acacCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGa > 1:4825841/1‑76 (MQ=255) acacCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGa > 2:7074268/1‑76 (MQ=255) cccGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGt < 2:7426123/76‑1 (MQ=255) ccGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTg > 2:3165583/1‑76 (MQ=255) ccGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTg > 2:3465536/1‑76 (MQ=255) ttCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTGTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTg > 2:5600816/1‑76 (MQ=255) ttCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTg < 1:3765954/76‑1 (MQ=255) ttCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTg > 2:2087345/1‑76 (MQ=255) ttCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTg < 2:291541/76‑1 (MQ=255) tctcACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTgg > 1:4983733/1‑76 (MQ=255) tctcACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTgg > 2:3013237/1‑76 (MQ=255) cTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGc > 2:5867507/1‑76 (MQ=255) | AGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGC > REL606/4163060‑4163206 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |