BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	762,305	C→G	intergenic (+77/‑56)	lysZ → / → lysQ	tRNA‑Lys/tRNA‑Lys

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	762,305	0	C	G	98.3%	863.9 / 3.9	237	intergenic (+77/‑56)	lysZ/lysQ	tRNA‑Lys/tRNA‑Lys
Reads supporting (aligned to +/- strand): ref base C (2/2); new base G (132/101); total (134/103)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.38e-01

Only 100 of 257 total aligned reads displayed.

TTTAAAGGTGGTTACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCCAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTT  >  REL606/762230‑762377

  tAAAGGTGGTTACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAg                                                                         >  1:1131161/1‑76 (MQ=255)

  tAAAGGTGGTTACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAg                                                                         <  1:1778760/76‑1 (MQ=255)

  tAAAGGTGGTTACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAg                                                                         >  2:3372097/1‑76 (MQ=255)

   aaaGGTGGTTACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGt                                                                        >  2:5188124/1‑76 (MQ=255)

   aaaGGTGGTTACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGt                                                                        >  1:644496/1‑76 (MQ=255)

    aaGGTGGTTACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTc                                                                       <  2:3037223/76‑1 (MQ=255)

     aGGTGGTTACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCa                                                                      <  2:3894657/76‑1 (MQ=255)

      ggtggtTACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCAt                                                                     >  1:6124838/1‑76 (MQ=255)

         gtttACTGGTAGAGAACGTGAAGGATAACGTGGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCt                                                                  <  2:2557354/74‑1 (MQ=17)

         ggtTACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCt                                                                  >  2:1371024/1‑76 (MQ=255)

          gtTACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCCAGTCATCCTg                                                                 <  2:6027016/76‑1 (MQ=255)

           ttACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGc                                                                >  2:4094487/1‑76 (MQ=255)

           ttACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGc                                                                >  1:2246709/1‑76 (MQ=255)

           ttACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGc                                                                >  2:2725221/1‑76 (MQ=255)

            tACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGGGAGACGAAGTCGAGTCATCCTGCa                                                               >  1:2746665/1‑76 (MQ=255)

            tACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCa                                                               >  2:1120344/1‑76 (MQ=255)

            tACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCa                                                               >  1:1673623/1‑76 (MQ=255)

             acgggTAGCGAAGGTGAGGGATACCGTTGCGTTGCCACCGGCCCGAAGGCCGAGACGAAGTCGAGTCATCCTGCAc                                                              <  2:5120642/73‑1 (MQ=9)

              cTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACg                                                             <  1:2912984/76‑1 (MQ=255)

              cTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACg                                                             <  2:7287652/76‑1 (MQ=255)

              cTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACg                                                             >  1:5187120/1‑76 (MQ=255)

               tGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGa                                                            <  1:3152245/76‑1 (MQ=255)

               tGGAAGAGAACGTGAAGGATAACGTTGCGTTAGCACCGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGa                                                            <  2:4157978/76‑1 (MQ=14)

                ggTAGAGAACGTGAAGGATAACGTTGCGTTAGCACCGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGAc                                                           <  1:438429/76‑1 (MQ=255)

                ggTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGAc                                                           >  2:5904967/1‑76 (MQ=255)

                 gTAGAGAACTTGAAGGATAACGTGGCGTTAGCAACGCCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGAcc                                                          <  1:2624007/76‑1 (MQ=14)

                 gTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGAcc                                                          >  2:859966/1‑76 (MQ=255)

                 gTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGAcc                                                          >  2:300230/1‑76 (MQ=255)

                  tAGAGAATGTGAAGGATAACGTTGCGTTAGCACCGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGAccc                                                         <  1:6668703/76‑1 (MQ=14)

                  tAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGAccc                                                         >  2:6310011/1‑76 (MQ=255)

                   agagAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGAcccc                                                        >  2:1524677/1‑75 (MQ=255)

                   agagAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGAcccc                                                        >  2:2288810/1‑75 (MQ=255)

                   agagAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACcca                                                        >  2:5721112/1‑76 (MQ=255)

                   agagAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACcca                                                        <  2:5455029/76‑1 (MQ=255)

                   agagAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACcca                                                        >  2:7005767/1‑76 (MQ=255)

                   agagAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACcca                                                        >  1:3429424/1‑76 (MQ=255)

                   agagAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACcca                                                        >  1:4853872/1‑76 (MQ=255)

                    gagaACGTGAAGGATAACGTTGCGTTAGCAACGCCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACccac                                                       <  2:3134692/76‑1 (MQ=255)

                     agaACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACccacc                                                      >  1:8124117/1‑76 (MQ=255)

                      gaACGTGAAGAATAACGTTGCGTTAGCAACGCCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACccacc‑a                                                    <  2:5929597/76‑1 (MQ=11)

                       aaCGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGGCGAGTCATCCTGCACGACCCACC‑At                                                   >  1:6149201/1‑76 (MQ=255)

                         cGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATcc                                                 >  2:3429334/1‑76 (MQ=255)

                           tGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTg                                               >  2:2235127/1‑76 (MQ=255)

                           tGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTg                                               >  1:3381996/1‑76 (MQ=255)

                           tGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTg                                               <  1:4014663/76‑1 (MQ=255)

                           tGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTg                                               >  1:3020010/1‑76 (MQ=255)

                            gAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGa                                              >  1:671687/1‑76 (MQ=255)

                            gAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGa                                              >  2:7104278/1‑76 (MQ=255)

                            gAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGa                                              >  2:3102686/1‑76 (MQ=255)

                            gAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGa                                              >  1:6852930/1‑76 (MQ=255)

                            gAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGa                                              >  2:4971568/1‑76 (MQ=255)

                             aaGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGaa                                             >  2:3981198/1‑76 (MQ=255)

                                   aaCGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTa                                       >  2:8006260/1‑76 (MQ=255)

                                   aaCGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTa                                       >  2:5816708/1‑76 (MQ=255)

                                    aCGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTaa                                      >  1:2316561/1‑76 (MQ=255)

                                      tttGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAag                                    <  2:5371978/75‑1 (MQ=255)

                                        tGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAagca                                  >  1:7109861/1‑76 (MQ=255)

                                        tGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAagca                                  >  2:2484418/1‑76 (MQ=255)

                                           gTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAgcagca                               >  1:1699631/1‑76 (MQ=255)

                                            ttACCAACGCCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCAt                              <  1:1867060/76‑1 (MQ=18)

                                             tAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCATa                             >  1:1537085/1‑76 (MQ=255)

                                             tAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCATa                             <  1:3666695/76‑1 (MQ=255)

                                              aGCAACGCCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCATaa                            <  1:6892420/76‑1 (MQ=255)

                                                cAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCATAATc                          >  2:3550886/1‑76 (MQ=255)

                                                cAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCATAATc                          >  2:198477/1‑76 (MQ=255)

                                                cAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCATAATc                          >  1:7230949/1‑76 (MQ=255)

                                                cAACGCCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCATAATc                          <  1:7742135/76‑1 (MQ=255)

                                                cAACGCCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCATAATc                          <  1:5853534/76‑1 (MQ=255)

                                                 aaCGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCATAATcc                         >  2:6200600/1‑76 (MQ=255)

                                                  aCGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCTACC‑ATCCTGAATGATTAAAGCAGCATAATccc                        <  2:5568498/76‑1 (MQ=255)

                                                   cgccccGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCATAATCCCg                       <  1:779061/73‑1 (MQ=255)

                                                   cGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCATAATCCCg                       >  2:1903615/1‑76 (MQ=255)

                                                     gCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCATAATCCCGCa                     <  2:4819988/76‑1 (MQ=255)

                                                      cccGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCATAATCCCGCaa                    >  1:965457/1‑76 (MQ=255)

                                                      cccGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCATAATCCCGCaa                    >  1:3918518/1‑76 (MQ=255)

                                                      cccGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCATAATCCCGCaa                    >  1:5892233/1‑76 (MQ=255)

                                                       ccGAGGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCATAATCCCGCAAg                   <  1:5596747/76‑1 (MQ=255)

                                                        cGAGGGGGGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCATAATCCCGCAAgg                  <  2:6423231/76‑1 (MQ=25)

                                                          aggggCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCATAATCCCGCAAgggg                <  1:4493921/74‑1 (MQ=255)

                                                          aaGGGGGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCATAATCCCGCAAgggg                <  1:1301295/76‑1 (MQ=255)

                                                           ggggCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCATAATCCCGCAAGGGGt               <  1:6253306/75‑1 (MQ=255)

                                                            gggCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCATAATCCCGCAAGGGGTc              <  2:6797814/76‑1 (MQ=255)

                                                               ggagaCGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCATAATCCCGCAAGGGGTCGtt           <  2:4356259/75‑1 (MQ=255)

                                                               ggagaCGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCATAATCCCGCAAGGGGTCGtt           <  1:196919/75‑1 (MQ=255)

                                                               cGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCATAATCCCGCAAGGGGTCGtt           >  1:6024616/1‑76 (MQ=255)

                                                                gagaCGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCATAATCCCGCAAGGGGTCGTTa          >  1:5539196/1‑76 (MQ=255)

                                                                 agaCGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCATAATCCCGCAAGGGGTCGTTAg         <  2:7838187/76‑1 (MQ=255)

                                                                 agaCGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCATAATCCCGCAAGGGGTCGTTAg         <  1:85789/76‑1 (MQ=255)

                                                                  gaCGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCATAATCCCGCAAGGGGTCGTTAGc        <  2:453777/76‑1 (MQ=255)

                                                                  gaCGAAGTCCAGCCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCATAATCCCGCAAGGGGTCGTTAGc        <  1:7920541/76‑1 (MQ=255)

                                                                    cGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCATAATCCCGCAAGGGGTCGTTAGCTc      <  1:6322017/76‑1 (MQ=255)

                                                                    cGAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCATAATCCCGCAAGGGGTCGTTAGCTc      <  1:2986325/76‑1 (MQ=255)

                                                                     gAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCATAATCCCGCAAGGGGTCGTTAGCTCa     <  2:2810756/76‑1 (MQ=255)

                                                                     gAAGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCATAATCCCGCAAGGGGTCGTTAGCTCa     >  2:1836638/1‑76 (MQ=255)

                                                                      aaGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAg    <  1:1026747/76‑1 (MQ=255)

                                                                      aaGTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAg    >  2:2856498/1‑76 (MQ=255)

                                                                      aaGTCGAGTCATCCTG‑ACCGCCCACCGATCCTGAATGCTTAAAGCAGCATAATCACGCAAGGGTGCGTTAGCTcct    >  2:2404342/1‑74 (MQ=255)

                                                                        gTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCTTAATCCCGCAAGGGGTCGTTAGCTCAGtt  <  2:2682825/76‑1 (MQ=255)

                                                                        gTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGtt  <  1:507467/76‑1 (MQ=255)

                                                                        gTCGAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGtt  <  1:7452294/76‑1 (MQ=255)

TTTAAAGGTGGTTACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCCAGTCATCCTGCACGACCCACC‑ATCCTGAATGATTAAAGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTT  >  REL606/762230‑762377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`