BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,193,779	G→T	L143I (CTT→ATT)	yeiB ←	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,193,779	0	G	T	100.0%	682.9 / NA	188	L143I (CTT→ATT)	yeiB	conserved inner membrane protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (68/120); total (68/120)

Only 100 of 201 total aligned reads displayed.

GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCT‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193706‑2193851

 ccGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCT‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATccc                                                                       <  1:6011236/76‑1 (MQ=255)

 ccGATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGGCCGGAATTAAGCCCCAA‑AAAAACAAaaacccaaaccc                                                                       >  2:3885620/1‑76 (MQ=255)

  cGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCT‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATcccc                                                                      <  2:4981396/76‑1 (MQ=255)

    attcATCCGGCGTCCAGGCGCGGCTGGTCTGGCT‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccac                                                                    <  2:5767940/73‑1 (MQ=255)

    aTGCATCCGGCGTCCAGGCGCGGCTGGTCTGGTT‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccac                                                                    <  2:4610189/76‑1 (MQ=255)

     tGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCT‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccacc                                                                   <  1:4805016/76‑1 (MQ=255)

      gCATCCGGCGTCCAGGCGCGGCTGGTCTGGCT‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccacca                                                                  <  2:2567557/76‑1 (MQ=255)

      gCATCCGGCGTCCAGGCGCGGCTGGTCTGGCT‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccacca                                                                  <  1:365618/76‑1 (MQ=255)

      gCATCCGGCGTCCAGGCGCGGCTGGGCTGGCT‑GTCGGGAAACAGCCCCCATAACAGCCAAAAGCCCAACCccccca                                                                  >  2:775702/1‑76 (MQ=255)

          ccGGCGTCCAGGCGCGGCTGGTCTGGCT‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATa                                                              <  1:7803475/76‑1 (MQ=255)

          ccGGCGTCCAGGCGCGGCTGGTCTGGCT‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATa                                                              <  1:4316440/76‑1 (MQ=255)

           cGGCGTCCAGGCGCGGCTGGTCTGGCT‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaa                                                             <  1:1485694/76‑1 (MQ=255)

           cGGCGTCCAGGCGCGGCTGGTCTGGCT‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaa                                                             <  1:5371713/76‑1 (MQ=255)

           cGGCGTCCAGGCGCGGCTGGTCTGGCT‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaa                                                             <  1:514582/76‑1 (MQ=255)

           cGGCGTCCAGGCGCGGCTGGTCTGGCT‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaa                                                             <  1:2189456/76‑1 (MQ=255)

            ggCGTCCAGGCGCGGCTGGTCTGGCT‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaaa                                                            <  2:262124/76‑1 (MQ=255)

            ggCGTCCAGGCGCGGCTGGTCTGGCT‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaaa                                                            <  1:6530552/76‑1 (MQ=255)

            ggCGTCCAGGCGCGGCTGGGCTGGCT‑GGCGGAAAACACCCCCAATAACAACAAAACGCCAAACCCCACCAGATaaa                                                            >  1:3138236/1‑76 (MQ=255)

              cGTCCAGGCGCGGCTGGGCTGGCT‑GTCGGAAAACAGCCCCCATAAAAACAAAAAGACAACTCCCACCCGGTAAAga                                                          >  2:5504301/1‑75 (MQ=255)

                   aGGCGCGGCTGGTCTGGCT‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGAc                                                     <  1:2303119/76‑1 (MQ=255)

                      cgcgGCTGGGCTGGCT‑GTCGGGAAACAGCCCCAATAAAAGCAAAACGCCCATCCCCACCAGATAAAGCATGACGcc                                                  >  2:3320732/1‑76 (MQ=255)

                         ggCTGGTCTGGCT‑GTCGGAAAACAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGt                                               >  1:929958/1‑76 (MQ=255)

                          gCTGGTCTGGCT‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTa                                              <  1:7409523/76‑1 (MQ=255)

                           cTGGTCTGGCT‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt                                             >  1:1496596/1‑76 (MQ=255)

                           cTGGTCTGGCT‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt                                             >  2:6835497/1‑76 (MQ=255)

                            tGGTCTGGCT‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            >  2:4664163/1‑76 (MQ=255)

                            tGGTCTGGCT‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            <  1:5311798/76‑1 (MQ=255)

                            tGGTCTGGCT‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            >  2:4064559/1‑76 (MQ=255)

                            tGGTCTGGCT‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            <  1:68339/76‑1 (MQ=255)

                            tGGTCTGGCT‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGACTGTAtt                                            >  1:4256745/1‑76 (MQ=255)

                             ggTCTGGCT‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                           >  2:6470719/1‑76 (MQ=255)

                              gTCTGGCT‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa                                          <  2:6973134/76‑1 (MQ=255)

                               tCTGGCT‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa                                         <  1:4968494/76‑1 (MQ=255)

                                cTGGCT‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                        <  2:1872411/76‑1 (MQ=255)

                                cTGGCT‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                        <  2:5739052/76‑1 (MQ=255)

                                 tGGCT‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa                                       >  1:4033354/1‑76 (MQ=255)

                                 tGGCT‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa                                       >  1:7619133/1‑76 (MQ=255)

                                   gCT‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAgg                                     <  2:4873792/76‑1 (MQ=255)

                                   gCT‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAgg                                     <  2:1463941/76‑1 (MQ=255)

                                    cT‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGc                                    >  2:3599066/1‑76 (MQ=255)

                                    cT‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGc                                    >  2:7707541/1‑76 (MQ=255)

                                    cT‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGc                                    >  2:8137682/1‑76 (MQ=255)

                                    cT‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGc                                    >  1:3802932/1‑76 (MQ=255)

                                     t‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt                                   <  2:7503316/76‑1 (MQ=255)

                                     t‑GTCAGAAATCAGCCCCAATAACAGCAAAACGCGAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt                                   >  2:3572920/1‑76 (MQ=255)

                                       gTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  <  2:3518499/76‑1 (MQ=255)

                                       gTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  <  2:2527443/76‑1 (MQ=255)

                                       gTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  <  2:4838698/76‑1 (MQ=255)

                                       gTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  <  2:2094077/76‑1 (MQ=255)

                                        tCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt                                 <  1:40662/76‑1 (MQ=255)

                                         cGGAAATCGGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                                <  2:4084682/76‑1 (MQ=255)

                                         cGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                                <  2:1289889/76‑1 (MQ=255)

                                         cGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                                >  2:3682814/1‑76 (MQ=255)

                                          ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                               <  2:2260026/76‑1 (MQ=255)

                                          ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                               <  1:1096705/76‑1 (MQ=255)

                                          ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                               >  1:5476344/1‑76 (MQ=255)

                                          ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                               <  1:1877659/76‑1 (MQ=255)

                                          ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                               <  1:6206162/76‑1 (MQ=255)

                                          ggAAAGCAGCACCAATAACAGCAAAACGTCAATCCCCCCCAGATAAAGCATGACCCCTGGAGTAAACAGGCttttt                               <  2:6414333/76‑1 (MQ=255)

                                           gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                              <  2:2341505/76‑1 (MQ=255)

                                           gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                              <  2:5571204/76‑1 (MQ=255)

                                           gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                              <  1:3753771/76‑1 (MQ=255)

                                            aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                             <  2:5992258/76‑1 (MQ=255)

                                            aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                             >  2:3189778/1‑76 (MQ=255)

                                            aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                             >  2:3069137/1‑76 (MQ=255)

                                             aaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAcc                            >  1:2669446/1‑76 (MQ=255)

                                              aTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg                           <  2:6176678/76‑1 (MQ=255)

                                              aTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg                           <  1:3496287/76‑1 (MQ=255)

                                               tCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGa                          <  2:7320628/76‑1 (MQ=255)

                                                cAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt                         <  2:4450342/76‑1 (MQ=255)

                                                 cgCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATg                        <  2:6591085/75‑1 (MQ=255)

                                                 aGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATg                        <  2:5125531/76‑1 (MQ=255)

                                                 aGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATg                        >  1:837380/1‑76 (MQ=255)

                                                  gCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATgg                       <  2:4580021/76‑1 (MQ=255)

                                                   ccccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc                      <  1:7320630/76‑1 (MQ=255)

                                                   ccccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc                      <  2:7090109/76‑1 (MQ=255)

                                                   ccccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc                      <  2:1557143/76‑1 (MQ=255)

                                                    cccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcg                     >  1:7862184/1‑76 (MQ=255)

                                                     ccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcgc                    >  1:7875810/1‑76 (MQ=255)

                                                      cAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCa                   >  1:7217250/1‑76 (MQ=255)

                                                       aaTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCAt                  <  1:1619679/76‑1 (MQ=255)

                                                       aaTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCAt                  >  1:2256878/1‑76 (MQ=255)

                                                        aTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc                 >  2:7860298/1‑76 (MQ=255)

                                                        aTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc                 >  2:1804662/1‑76 (MQ=255)

                                                        aTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc                 >  1:7724524/1‑76 (MQ=255)

                                                         tAACAGCAAACCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg                >  1:1787875/1‑76 (MQ=255)

                                                         tAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg                >  2:1626971/1‑76 (MQ=255)

                                                         tAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg                <  1:2951268/76‑1 (MQ=255)

                                                          aaCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc               >  1:3427490/1‑76 (MQ=255)

                                                          aaCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc               >  1:5646548/1‑76 (MQ=255)

                                                          aaCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc               >  1:7064662/1‑76 (MQ=255)

                                                            cAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCgcgc             <  2:2755446/76‑1 (MQ=255)

                                                              gCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAc           >  2:338744/1‑76 (MQ=255)

                                                              gCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAc           <  2:3987867/76‑1 (MQ=255)

                                                                aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         <  2:2170003/76‑1 (MQ=255)

                                                                aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         <  1:4654708/76‑1 (MQ=255)

                                                                aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         <  2:4100717/76‑1 (MQ=255)

                                                                  aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc       >  2:6356733/1‑76 (MQ=255)

                                                                     gCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgc    >  2:2868380/1‑76 (MQ=255)

                                                                      ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc   >  2:7685581/1‑76 (MQ=255)

GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCT‑GTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193706‑2193851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`