| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,911,991 | (T)5→4 | intergenic (‑192/‑131) | yifB ← / → ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,911,987 | 0 | T | . | 92.1% | 590.6 / 49.6 | 141 | intergenic (‑188/‑135) | yifB/ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
| Reads supporting (aligned to +/- strand): ref base T (4/7); new base . (55/74); total (60/81) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 | |||||||||||
Only 100 of 159 total aligned reads displayed.
GCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTAC > REL606/3911913‑3912058 | ggACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTattttatttt < 2:1919241/76‑1 (MQ=255) ggACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTattttatttt < 2:2043838/76‑1 (MQ=255) aCAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTattttattttaa > 1:45372/1‑74 (MQ=255) aCAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTATTTTTa > 1:1554215/1‑76 (MQ=255) cAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTATTTTaaa < 1:785639/76‑1 (MQ=255) cAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTATTTTaaa < 2:489922/76‑1 (MQ=255) cAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTATTTTaaa > 2:4384669/1‑76 (MQ=255) aaaGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTATTTTaaaa < 1:4372996/76‑1 (MQ=255) aaaGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTATTTTaaaa < 2:1606090/76‑1 (MQ=255) aaGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTATTTTAaaaa < 1:778258/76‑1 (MQ=255) ggATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTATTTTaaaaaac > 2:832949/1‑75 (MQ=255) gATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAaaca > 1:893067/1‑76 (MQ=255) aTGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAaacaa > 1:3600658/1‑76 (MQ=255) aTGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAaacaa < 2:4415330/76‑1 (MQ=255) tGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAaacaac < 1:3720377/76‑1 (MQ=255) tGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAaacaac < 2:975898/76‑1 (MQ=255) tGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAaacaac < 2:1095947/76‑1 (MQ=255) tGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAaacaac < 1:4224367/76‑1 (MQ=255) gAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAacaaca < 2:2505458/76‑1 (MQ=255) gAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAacaaca < 1:4681748/76‑1 (MQ=255) gAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAacaaca < 1:2587245/76‑1 (MQ=255) aaCTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAAcaacaa < 2:2443296/76‑1 (MQ=255) aaCTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAAcaacaa > 2:4162846/1‑76 (MQ=255) aaCTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAAcaacaa < 2:1006895/76‑1 (MQ=255) aCTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAAt < 1:2536743/76‑1 (MQ=255) aCTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAAt < 2:3599427/76‑1 (MQ=255) aCTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAAt < 1:1009925/76‑1 (MQ=255) cTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAAtt > 1:4945285/1‑76 (MQ=255) cTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAAtt < 1:4582933/76‑1 (MQ=255) tACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAAttt < 2:1576295/76‑1 (MQ=255) gAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTtata < 1:3044186/76‑1 (MQ=255) gAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTtata < 2:2190721/76‑1 (MQ=255) gAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTtata > 2:252561/1‑76 (MQ=255) aGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTatat > 2:4822228/1‑76 (MQ=255) ggAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATAtt < 1:1587717/76‑1 (MQ=255) aaGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGa < 1:1033898/76‑1 (MQ=255) aGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGaa < 2:1990145/76‑1 (MQ=255) aGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGaa > 2:1834501/1‑76 (MQ=255) aacaacATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAatta > 2:4007473/1‑76 (MQ=255) aacaTTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAAttatta < 2:4472456/76‑1 (MQ=255) aacaTTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAAttatta > 1:3671680/1‑76 (MQ=255) aacaTTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAAttatta < 1:2937191/76‑1 (MQ=255) aacaTTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAAttatta > 1:561530/1‑76 (MQ=255) caTTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTaaa < 2:1775003/76‑1 (MQ=255) caTTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTaaa > 2:456896/1‑76 (MQ=255) caTTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTaaa < 2:2711779/76‑1 (MQ=255) caTTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTaaa < 1:4169787/76‑1 (MQ=255) aTTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAAc > 2:2809802/1‑76 (MQ=255) ttCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACg < 1:4484331/76‑1 (MQ=255) tCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGc < 1:61345/76‑1 (MQ=255) aTACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcat < 1:1475813/76‑1 (MQ=255) tACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcatc > 1:2060092/1‑76 (MQ=255) tACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcatc > 1:2498991/1‑76 (MQ=255) tACTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcatc < 1:2787457/76‑1 (MQ=255) aCTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcatca > 2:4278415/1‑76 (MQ=255) aCTGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcatca < 1:1691410/76‑1 (MQ=255) cTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcatc < 1:1508911/76‑1 (MQ=255) tGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATa < 2:309910/76‑1 (MQ=255) tGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATa > 1:2684083/1‑76 (MQ=255) tGAAATTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATa < 2:4732293/76‑1 (MQ=255) aaaTTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaa < 1:3111458/76‑1 (MQ=255) aaaTTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaa < 2:2835829/76‑1 (MQ=255) aaaTTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaa < 1:3302525/76‑1 (MQ=255) aaTTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaaa < 2:1697685/76‑1 (MQ=255) aaTTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaaa < 2:2603004/76‑1 (MQ=255) aTTGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaaaa > 1:4680755/1‑76 (MQ=255) aTTGAA‑TTTGTTTCGCTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaaaa < 1:1239307/76‑1 (MQ=255) ttGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAAt > 2:1205952/1‑76 (MQ=255) ttGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAAt < 1:4498666/76‑1 (MQ=255) ttGAA‑TTTTTTTCACTCACTATTTT‑GTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAAt > 1:5004415/1‑76 (MQ=255) tGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATc > 2:920909/1‑76 (MQ=255) tGAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATc > 1:891672/1‑76 (MQ=255) gAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCg > 1:3404391/1‑76 (MQ=255) gAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCg > 1:4898908/1‑76 (MQ=255) gAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCg > 1:1622107/1‑76 (MQ=255) gAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCg < 2:3378945/76‑1 (MQ=255) gAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCg > 2:4510438/1‑76 (MQ=255) gAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCg > 1:185942/1‑76 (MQ=255) gAA‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCg > 1:3223622/1‑76 (MQ=255) aa‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCgg > 2:3303005/1‑76 (MQ=255) aa‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCgg > 2:3886056/1‑76 (MQ=255) aa‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCgg < 2:605818/76‑1 (MQ=255) a‑TTTTTTTCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGc < 2:522990/76‑1 (MQ=255) ttttttCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCa < 2:2722452/76‑1 (MQ=255) ttttttCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCa > 1:3929752/1‑76 (MQ=255) ttttttCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCa > 1:1349111/1‑76 (MQ=255) ttttCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaaa < 1:961249/76‑1 (MQ=255) tttCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaaaa > 2:3535052/1‑76 (MQ=255) tttCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaaaa < 2:621883/76‑1 (MQ=255) ttCACTCACTATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaaaaa < 1:669997/76‑1 (MQ=255) ctcactATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAatat < 1:4105293/76‑1 (MQ=255) cactATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCt > 1:2559957/1‑76 (MQ=255) ctATTTTA‑TTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTg > 1:3376093/1‑76 (MQ=255) tatttta‑tttTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGt > 1:2238022/1‑76 (MQ=255) tatttta‑tttTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGt > 1:1152025/1‑76 (MQ=255) attttattttTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGt < 1:3247538/76‑1 (MQ=255) atttta‑ttttAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTa > 1:1254620/1‑76 (MQ=255) atttta‑ttttAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTa > 1:1072899/1‑76 (MQ=255) ttttattttTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTa < 2:1021382/76‑1 (MQ=255) tttta‑ttttaAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTAc > 1:1965544/1‑76 (MQ=255) | GCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTAC > REL606/3911913‑3912058 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |