| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,822,155 | (A)8→7 | intergenic (‑56/+72) | ynjI ← / ← topB | predicted inner membrane protein/DNA topoisomerase III |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,822,148 | 0 | A | . | 97.3% | 531.2 / 6.9 | 110 | intergenic (‑49/+79) | ynjI/topB | predicted inner membrane protein/DNA topoisomerase III |
| Reads supporting (aligned to +/- strand): ref base A (1/2); new base . (49/58); total (50/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 134 total aligned reads displayed.
ATGGTTTTGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAGATT > REL606/1822073‑1822219 | aTGGTTTTGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCa > 1:3587302/1‑76 (MQ=255)aTGGTTTTGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCa < 2:459084/76‑1 (MQ=255)aTGGTTTTGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCa > 2:1393106/1‑76 (MQ=255)aTGGTTTTGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCa < 1:73755/76‑1 (MQ=255) tGGTTTTGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCaa > 1:2895138/1‑76 (MQ=255) tGGTTTTGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCaa < 2:3891547/76‑1 (MQ=255) tGGTTTTGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCaa < 2:3624969/76‑1 (MQ=255) ggTTTTGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCaaa > 2:3593874/1‑76 (MQ=255) ggTTTTGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCaaa > 1:4193084/1‑76 (MQ=255) ggTTTTGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCaaa < 2:2894086/76‑1 (MQ=255) ttttGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCaaaaa > 2:2822787/1‑76 (MQ=255) ttttGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCaaaaa < 1:2638121/76‑1 (MQ=255) ttGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCaaaaaaa > 2:1162524/1‑76 (MQ=255) tGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCaaaaaaat < 2:1828349/76‑2 (MQ=255) gTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCaaaaaaata > 1:555071/1‑74 (MQ=255) gTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCaaaaaaata > 1:3890178/1‑74 (MQ=255) taataaAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCaaaaaaatat < 2:29907/76‑4 (MQ=255) taataaAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCaaaaaaatat > 2:4281330/1‑73 (MQ=255) taataaAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCaaaaaaatat > 1:1273308/1‑73 (MQ=255) ataaAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATcc > 1:5002373/1‑76 (MQ=255) ataaAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATcc < 2:1220422/76‑1 (MQ=255) taaAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCa < 1:4716779/76‑1 (MQ=255) taaAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCa < 2:3506896/76‑1 (MQ=255) taaAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCa < 1:959673/76‑1 (MQ=255) taaAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCa < 1:174993/76‑1 (MQ=255) taaAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCa < 1:1797722/76‑1 (MQ=255) aaaaCTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCa < 1:1148683/76‑1 (MQ=255) aaaaCTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCaa < 2:3291773/76‑1 (MQ=255) aaaaCTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCaa < 2:698449/76‑1 (MQ=255) aaCTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACg < 1:4011335/76‑1 (MQ=255) cTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGaa < 1:2513152/76‑1 (MQ=255) cTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGaa < 1:1730212/76‑1 (MQ=255) ttttttCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAAt > 2:344825/1‑76 (MQ=255) tttttCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAAtt > 2:2524442/1‑76 (MQ=255) ttttCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAAttt > 2:4947874/1‑76 (MQ=255) tttCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAAtttt > 1:2058976/1‑76 (MQ=255) tttCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAAtttt > 1:2510141/1‑76 (MQ=255) tcatcaCATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCt > 1:1490973/1‑76 (MQ=255) tcatcaCATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCt > 2:3603555/1‑76 (MQ=255) catcaCATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCtt > 2:4367612/1‑76 (MQ=255) catcaCATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCtt < 1:3660276/76‑1 (MQ=255) catcaCATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCtt > 1:3505969/1‑76 (MQ=255) tcaCATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGa > 1:1471516/1‑76 (MQ=255) acaTTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATc < 2:197408/76‑1 (MQ=255) aTTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCg > 2:3917702/1‑76 (MQ=255) ttCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGc > 2:778628/1‑76 (MQ=255) tCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCt < 2:4977125/76‑1 (MQ=255) tCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCt > 2:2395305/1‑76 (MQ=255) ccTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTgg < 2:3056320/76‑1 (MQ=255) ccTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTgg < 1:3593874/76‑1 (MQ=255) cTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGATCCGCTgg > 2:2666308/1‑76 (MQ=255) cTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGa > 1:260591/1‑76 (MQ=255) cTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGa > 2:462986/1‑76 (MQ=255) tGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGat < 2:648914/76‑1 (MQ=255) tGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGat > 1:3208500/1‑76 (MQ=255) gTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGata > 2:2931589/1‑76 (MQ=255) gTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGata < 2:525546/76‑1 (MQ=255) gTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGata < 2:2640567/76‑1 (MQ=255) tattaCATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATAtt < 2:3199318/76‑1 (MQ=255) tattaCATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATAtt < 2:2306608/76‑1 (MQ=255) tattaCATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATAtt > 1:4506293/1‑76 (MQ=255) attaCATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCTAACGAATTTTCTTGATCCGCTGGATAttt > 1:4718998/1‑76 (MQ=255) attaCATACTTTAGAGGCGATAATTTTAACCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATAttt < 2:1161190/76‑1 (MQ=255) ttaCATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTc > 1:4869568/1‑76 (MQ=255) ttaCATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTc < 2:1399155/76‑1 (MQ=255) aCATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAg > 2:42533/1‑76 (MQ=255) aCTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAata > 2:1534035/1‑76 (MQ=255) aCTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAata > 1:503571/1‑76 (MQ=255) ttAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAGTATGa < 2:4867197/76‑1 (MQ=255) ttAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGa < 1:3861721/76‑1 (MQ=255) agagGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACt > 1:2669957/1‑76 (MQ=255) agagGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACt > 1:3755556/1‑76 (MQ=255) gagGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTc < 1:1971772/76‑1 (MQ=255) agGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCg < 2:2263055/76‑1 (MQ=255) cGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCGAAt < 2:1471516/76‑1 (MQ=255) cGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCGAAt < 2:4751537/76‑1 (MQ=255) gATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCGAATa < 1:1546780/76‑1 (MQ=255) gATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCGAATa < 1:4082068/76‑1 (MQ=255) aTAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCGAATAg < 2:3083172/76‑1 (MQ=255) aTAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCGAATAg < 2:426702/76‑1 (MQ=255) aTTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCGAATAGCAc < 2:7762/76‑1 (MQ=255) ttttATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCGAATAGCACg < 1:2112982/76‑1 (MQ=255) ttttATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCGAATAGCACg < 2:1482516/76‑1 (MQ=255) ttttATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCCAATAGCaag > 1:4350711/1‑74 (MQ=255) ttttATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCCAATAGCACg > 2:4078764/1‑76 (MQ=255) tttATCCATGC‑AAACAAATATCCAACGAATTTTCTTGATCCGCTGGACATTTCAGAATATGACTCGAATAGCAcca > 2:3236483/1‑74 (MQ=255) tttATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCGCATAGCACGa > 2:1040857/1‑76 (MQ=255) tttATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCGAACAGCAAGa > 2:3374000/1‑76 (MQ=255) tttATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCCAATAGCACGa > 1:4482300/1‑76 (MQ=255) ttATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCGAATAGCACGaa < 1:2953902/76‑1 (MQ=255) ttATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCGAATAGCACGaa > 2:1951802/1‑76 (MQ=255) ttATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCGAATAGACCGaa > 2:4502972/1‑76 (MQ=255) tATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCGAATAGCACGaaa < 1:2986942/76‑1 (MQ=255) aTCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAg < 1:471149/76‑1 (MQ=255) aTCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAg < 1:4931337/76‑1 (MQ=255) aTCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAg < 1:916372/76‑1 (MQ=255) aTCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAg < 1:2235028/76‑1 (MQ=255) aTCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCGAATAGAACGCAAg > 2:4310340/1‑76 (MQ=255) ccATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAAAt > 1:3327883/1‑76 (MQ=255) cATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAGAtt > 1:1276547/1‑76 (MQ=255) | ATGGTTTTGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCGAATAGCACGAAAGATT > REL606/1822073‑1822219 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |