| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,131,466 | (A)9→8 | intergenic (‑125/+175) | gatY ← / ← fbaB | D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,131,458 | 0 | A | . | 97.1% | 323.3 / 1.1 | 68 | intergenic (‑117/+183) | gatY/fbaB | D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base . (54/12); total (55/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 127 total aligned reads displayed.
AAATGATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACATA > REL606/2131383‑2131531 | aaatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCa > 2:1627661/1‑76 (MQ=255)aaatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCa > 2:1809963/1‑76 (MQ=255)aaatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCa < 2:2443575/76‑1 (MQ=255)aaatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCa > 2:3582257/1‑76 (MQ=255)aaatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCa < 2:1265098/76‑1 (MQ=255) aatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaa < 1:2914009/76‑1 (MQ=255) aatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaa < 1:4147555/76‑1 (MQ=255) aatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaa > 1:3953927/1‑76 (MQ=255) aatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaa > 2:2334256/1‑76 (MQ=255) aatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaa < 1:4713476/76‑1 (MQ=255) aatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaa < 1:1027066/76‑1 (MQ=255) atgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaa > 1:8888/1‑76 (MQ=255) tgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaa < 1:3388452/76‑1 (MQ=255) gaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaa > 1:2191201/1‑76 (MQ=255) aTTTGTTTTTAAATGTCGAGATAACGATCACAACAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaa > 1:4863977/1‑76 (MQ=255) aTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaa > 2:4265386/1‑76 (MQ=255) aTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaa > 2:490355/1‑76 (MQ=255) tttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTTATAGAGGAATGAAAGGCaaaaaaa > 2:372972/1‑76 (MQ=255) tttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaa < 1:1205616/76‑1 (MQ=255) tttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaa < 2:623802/76‑1 (MQ=255) tttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaa > 1:3321190/1‑76 (MQ=255) ttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaa > 1:1496173/1‑76 (MQ=255) tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaac > 2:531527/1‑75 (MQ=255) tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaac > 1:887820/1‑75 (MQ=255) tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaac < 2:458178/76‑2 (MQ=255) gTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaacg < 1:2175816/76‑3 (MQ=255) tttttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaacgg > 1:3899085/1‑73 (MQ=255) tttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGcc > 2:4883057/1‑76 (MQ=255) tttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGcc > 2:1467670/1‑76 (MQ=255) tttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGcc > 1:249554/1‑76 (MQ=255) aaaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTcc > 1:1328659/1‑76 (MQ=255) aaaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTcc > 1:828588/1‑76 (MQ=255) aaaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTcc > 1:4017385/1‑76 (MQ=255) aaaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAAGC‑AAAAAAAACGGCCTcc < 2:3284951/76‑1 (MQ=255) aTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCg > 2:1591635/1‑76 (MQ=255) aTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCg < 2:3940249/76‑1 (MQ=255) tGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCgg > 2:1800447/1‑76 (MQ=255) tGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCgg > 2:4131386/1‑76 (MQ=255) gTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGa < 2:1237466/76‑1 (MQ=255) cGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAgg < 2:1445799/76‑1 (MQ=255) cGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAgg > 1:1473820/1‑76 (MQ=255) gagaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAggg < 1:3290980/76‑1 (MQ=255) agaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGa > 2:1323605/1‑76 (MQ=255) agaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGa > 2:3712411/1‑76 (MQ=255) agaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGa < 2:65226/76‑1 (MQ=255) agaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGa > 1:1517576/1‑76 (MQ=255) agaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGa > 1:4097306/1‑76 (MQ=255) gaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGaa < 1:3712609/76‑1 (MQ=255) aTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAg < 2:2202510/76‑1 (MQ=255) aTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAg < 2:1615367/76‑1 (MQ=255) tAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGc > 2:2525368/1‑76 (MQ=255) tAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGc > 1:3661061/1‑76 (MQ=255) tAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGc > 1:2688584/1‑76 (MQ=255) aaCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGcc < 2:1884616/76‑1 (MQ=255) aaCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGcc > 2:4100899/1‑76 (MQ=255) cGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGc > 2:4264962/1‑76 (MQ=255) aTCAAAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGG‑AAAAAAAAACGGCCCCCCGGAGGGAAGCCGcaa < 2:3517488/76‑1 (MQ=255) tCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGcaac > 2:3944440/1‑76 (MQ=255) acaAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGcaacaa > 1:1241216/1‑76 (MQ=255) acaAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGcaacaa > 1:2041374/1‑76 (MQ=255) caAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACaaa > 1:4366483/1‑76 (MQ=255) aaaCGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGt > 2:2628966/1‑76 (MQ=255) aaaCAAAAATATAAAAATTAAAAGAGAAAAAAAAGG‑AAAAAAAACCGCCCCCCGGGGGGAAAGCCGCAACAAAGTg < 2:2673377/76‑1 (MQ=255) aaaCAAAAATATAAAAATAAAAAGGGGAATAAAAGG‑AAAAAAAACCGCCCCCCCGGGGGGAAGCCGCAACAAAGTg < 2:440406/76‑1 (MQ=255) aCGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTgcg > 2:3372458/1‑76 (MQ=255) cGACAATATGAAAATTAATAGAGGATTGACTGGCAAAAAAACACGGCCTCCCGGAGGGCAGCCGCAAAAACGTgcg > 2:4810168/1‑76 (MQ=255) aCAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGt > 2:176351/1‑76 (MQ=255) aCAATATGAAAATTAATAGAGGAATGAAAGG‑AAAAAAAAACGGCCCCCCGGAGGGAAGCCGCAACAAAGTGCGCGt < 1:4891443/76‑1 (MQ=255) aaTATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTgg > 2:1348982/1‑76 (MQ=255) aaTATAAAAATTAATAGAGGAAT‑AAAAGCAAAAAAAACCGGCCCCCCGGGGGAAAGCCGCAACAAAGTGCGCGTgg < 2:3395446/76‑1 (MQ=255) aaTATAAAAATAAATAGAGAAATAAAAGG‑AAAAAAAACCGGCCCCCCGGAGGGAAGCCGCAACAAAGTGCGCGTgg < 1:4076377/76‑1 (MQ=255) atatGAAAATAAATAGAGAAATGAAATAAAAAAAAAACCGCCCTCCCGGAGGAAAGCCGCAACAAAGTGCGCGTgg < 1:516569/76‑1 (MQ=255) tatGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGtt > 1:747598/1‑76 (MQ=255) ataaaaaTTAATAGAGAAAT‑AAAAGAAAAAAAAACCGCCCCCCCGGAGGAAAGCCGCAACAAAGTGCGCGTGGttt < 2:3044559/73‑1 (MQ=255) aaaaaaaTAAAGAGA‑GAAAGAAAAGAAAAAAAAACCGCCCCCCGGGAGGAAAGCCGCAACAAAGTGCGCGTGGttt < 2:1371705/73‑1 (MQ=255) tGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGtttt > 2:2227232/1‑76 (MQ=255) aaaaaaTTAAAGGAGAAAT‑AAAAGAAAAAAAAACCGGCCCCCGGGAGGAAAGCCGCAACAAAGTGCGCGTGGtttt < 2:4772010/74‑1 (MQ=255) gAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTa > 1:3833763/1‑76 (MQ=255) gAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTa > 1:4452969/1‑76 (MQ=255) gAAAATAAATAGAGGAATAAAAGGC‑AAAAAAACCGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTa < 1:3281980/76‑1 (MQ=255) aaaaTAAATAG‑GGAAGTAAAGGAAAAAAAAACCGCCCCCCCGGAGGAAAGCCGCAACAAAGTGCGCGTGGTTTTAt < 2:2160868/76‑1 (MQ=255) aaTTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGc > 2:2032575/1‑76 (MQ=255) aaTTAAAAGAGGAATGAAA‑GAAAAAAAAACCGGCCCCCGGGAGGAAAGCCGCAACAAAGTGCGCGTGGTTTTATGc < 2:2094843/76‑1 (MQ=255) ataaaTAGAGAAATGAAAGG‑AAAAAAACCCGGCCTCCCGGAGGAAAGCCGCAACAAAGTGCGCGTGGTTTTATGCa < 1:2518215/73‑1 (MQ=255) aTTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCa > 1:3099766/1‑76 (MQ=255) aTTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCa > 1:2840996/1‑76 (MQ=255) tAATAGAGGAATGAAAGGC‑AAAAAAACCGCCCCCCCGGAGGAAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAgg < 1:4265386/76‑1 (MQ=255) tAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAgg > 2:2874196/1‑76 (MQ=255) tAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAgg > 2:1570763/1‑76 (MQ=255) tAAAAGAGGAATAAAAGG‑AAAAAAAACCGCCCCCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAgg < 1:2791616/76‑1 (MQ=255) aaTAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGa > 2:1105382/1‑76 (MQ=255) agagGAATGAAA‑GAAAAAAAAACCGCCCCCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgc < 1:3283949/76‑1 (MQ=255) gAATGAAA‑GAAAAAAAAACCGGCCCCCGGGAGGAAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcggcg < 2:145391/76‑1 (MQ=37) aTGAAAGAC‑AAAAAAACCGGCCTCCCGGGGGAAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTa < 2:1058235/76‑1 (MQ=37) tGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTaa > 1:1160307/1‑76 (MQ=255) aaaGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAAc > 2:4095960/1‑76 (MQ=255) aaaGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAAc > 1:3308218/1‑76 (MQ=255) aaaGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAAc > 2:2030404/1‑76 (MQ=255) aaaGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGGGCGCGTGGTTTTATGCAGGATGCGGCGTAAAc > 2:1036054/1‑76 (MQ=38) ggaaaaaaaaaCGCCCCCCGGGAGGAAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACata < 1:2239134/74‑1 (MQ=37) | AAATGATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACATA > REL606/2131383‑2131531 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |