Predicted mutation
evidence seq id position mutation annotation gene description
MC JC REL606 3,894,997 Δ6,934 bp IS150‑mediated rbsD–[yieO] rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ REL606 3893606–3894994 3901930 6937–8325 55 [50] [0] 184 [insJ‑5]–[yieO] [insJ‑5],insK‑5,rbsD,rbsA,rbsC,rbsB,rbsK,rbsR,[yieO]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 = 3652533NA (NA)175 (1.200) 88/146 0.1 100% noncoding (1443/1443 nt) IS150 repeat region
?REL606 3901931 = 0 (0.000)coding (489/1428 nt) yieO predicted multidrug or homocysteine efflux system

TCAGGTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAA  >  REL606/3893530‑3893627
                                                                           |                      
tCAGGTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTc                        >  1:4825925/1‑76 (MQ=255)
tCAGGTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTc                        >  2:3114721/1‑76 (MQ=255)
tCAGGTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTc                        <  2:830122/76‑1 (MQ=255)
tCAGGTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTc                        <  1:4013418/76‑1 (MQ=255)
tCAGGTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTc                        >  1:3920569/1‑76 (MQ=255)
 cAGGTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCa                       >  1:1198154/1‑76 (MQ=255)
 cAGGTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCa                       >  2:158820/1‑76 (MQ=255)
   ggTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCaaa                     <  2:961359/76‑1 (MQ=255)
   ggTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCaaa                     <  1:646654/76‑1 (MQ=255)
   ggTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCaaa                     <  2:4334938/76‑1 (MQ=255)
    gTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAg                    <  1:537238/76‑1 (MQ=255)
    gTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAg                    <  1:2548854/76‑1 (MQ=255)
    gTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAg                    >  2:3837640/1‑76 (MQ=255)
    gTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAg                    <  1:1165218/76‑1 (MQ=255)
      cGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGcc                  >  1:907837/1‑76 (MQ=255)
      cGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGcc                  >  2:2829898/1‑76 (MQ=255)
      cGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGcc                  >  1:4457988/1‑76 (MQ=255)
       gAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCa                 <  1:394777/76‑1 (MQ=255)
       gAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCa                 >  1:4572713/1‑76 (MQ=255)
        aaaTCTAACGCCAGACTGTACTGCACCCATTTTTTTGGACGATGAAATGGCATAGACACTCATATGTCAAAGCCaa                >  2:2992545/1‑76 (MQ=17)
        aaaTCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCaa                >  2:4423482/1‑76 (MQ=255)
        aaaTCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCaa                <  1:1654064/76‑1 (MQ=255)
        aaaTCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCaa                >  2:1864735/1‑76 (MQ=255)
        aaaTCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCaa                >  2:2992544/1‑76 (MQ=255)
        aaaTCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCaa                <  1:2232459/76‑1 (MQ=255)
           tCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAAt             >  1:20067/1‑76 (MQ=255)
           tCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAAt             <  2:4931948/76‑1 (MQ=255)
           tCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAAt             <  1:311/76‑1 (MQ=255)
           tCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAAt             <  1:624847/76‑1 (MQ=255)
           tCTAACGACAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAAt             <  1:4687407/76‑1 (MQ=255)
            cTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATa            >  1:5003618/1‑76 (MQ=255)
            cTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATa            <  1:3838521/76‑1 (MQ=255)
            cTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATa            >  1:4075582/1‑76 (MQ=255)
            cTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATa            <  1:4327183/76‑1 (MQ=255)
             tAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATAc           >  2:3335692/1‑76 (MQ=255)
              aaCGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATAcc          <  2:1247376/76‑1 (MQ=255)
              aaCGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATAcc          >  2:3995011/1‑76 (MQ=255)
              aaCGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATAcc          >  2:2252956/1‑76 (MQ=255)
              aaCGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATAcc          >  2:2624873/1‑76 (MQ=255)
                cGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCt        <  1:664942/76‑1 (MQ=40)
                cGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCt        <  1:2452210/76‑1 (MQ=40)
                 gCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCtt       >  2:2827047/1‑76 (MQ=39)
                 gCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCtt       <  2:3789204/76‑1 (MQ=39)
                  ccAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCttt      >  2:1482476/1‑76 (MQ=38)
                   cAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCtttt     >  1:38965/1‑76 (MQ=35)
                    aGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTg    <  2:501442/76‑1 (MQ=34)
                    aGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTg    >  1:1049957/1‑76 (MQ=34)
                     gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   >  1:430102/1‑76 (MQ=32)
                     gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   >  1:4526456/1‑76 (MQ=32)
                      aCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGaa  <  1:2231995/76‑1 (MQ=31)
                      aCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGaa  >  1:1081775/1‑76 (MQ=31)
                      aCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGaa  <  1:3498013/76‑1 (MQ=31)
                      aCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGaa  <  1:4365084/76‑1 (MQ=31)
                      aCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGaa  <  1:4505973/76‑1 (MQ=31)
                      aCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGaa  <  1:806253/76‑1 (MQ=31)
                                                                           |                      
TCAGGTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAA  >  REL606/3893530‑3893627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑